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Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology)
Published by Universitas Diponegoro
ISSN : 25276751     EISSN : 25410083     DOI : -
Core Subject : Health,
Arjuna Subject : -
Articles 2 Documents
Search results for , issue "Volume 11, Nomor 1, Tahun 2026" : 2 Documents clear
Perbandingan Kekuatan Antibakteri Minyak Atsiri dari Tiga Varietas Jahe (Zingiber officinale Rosc) Terhadap Baccillus subtilis dan Pseudomonas aeruginosa Kristiani, Elizabeth Betty Elok; Kaya, Emma Sharon Admatha; Kasmiyati, Sri
Buletin Anatomi dan Fisiologi Volume 11, Nomor 1, Tahun 2026
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.1.1.2026.9-15

Abstract

Jahe (Zingiber officinale) merupakan tanaman rempah yang banyak tumbuh di Indonesia dan diketahui memiliki potensi sebagai agen antibakteri. Penelitian ini bertujuan untuk membandingkan aktivitas antibakteri minyak atsiri dari tiga varietas jahe yaitu jahe gajah (Z. officinale var. Roscoe), jahe merah (Z. officinale var Rubrum), dan jahe emprit (Z. officinale var Amarum)  terhadap bakteri Bacillus subtilis dan Pseudomonas aeruginosa. Ekstraksi minyak atsiri menggunakan teknik sohkletasi dengan pelarut etanol. Uji aktivitas antibakteri menggunakan metode difusi cakram. Rendemen minyak atsiri tertinggi Z. officinale var Rubrum 23,45 ± 3,32 %, diikuti Z. officinale var Amarum 19,90 ± 2,17 % dan terendah Z. officinale var. Roscoe 15,70 ± 2,42 %. Pada semua konsentrasi uji, kemampuan minyak atsiri jahe dalam menghambat pertumbuhan Baccillus subtilis lebih kecil secara signifikan dibandingkan tetrasiklin dalam tetapi lebih besar pada P. aeruginosa. Hasil uji antibakteri minyak atsiri semua tiga varietas jahe pada konsentrasi 62,6 – 1000 µg/mL terbukti menghambat pertumbuhan kedua bakteru uji dalam kategori kuat yaitu diamter daya hambat > 20 mm. Kekuatan aktivitas antibakteri minyak atsiti terhadap B. Subtilis lebih rendah dari antibiotika standar tetrasiklin, sedangkan terhadap P. aeruginosa lebih kuat. Minyak atsiri ketiga variets berpotensi untuk dijadikan sebagai agen antibakteri terhadap strain gram negatif dan gram positif.  Ginger (Zingiber officinale) is a spice plant that grows widely in Indonesia and is known to have potential as an antibacterial agent. This study aims to compare the antibacterial activity of essential oils from three varieties of ginger, namely elephant ginger (Z. officinale var. Roscoe), red ginger (Z. officinale var Rubrum), and emprit ginger (Z. officinale var Amarum) against Bacillus subtilis and Pseudomonas aeruginosa bacteria. Essential oil extraction was performed using the Soxhlet extraction technique with ethanol as the solvent. Antibacterial activity was tested using the disk diffusion method. The highest essential oil yield was obtained from Z. officinale var. Rubrum at 23.45 ± 3.32%, followed by Z. officinale var. Amarum at 19.90 ± 2.17% and the lowest from Z. officinale var. Roscoe at 15.70 ± 2.42%. At all test concentrations, the ability of ginger essential oil to inhibit the growth of Bacillus subtilis was significantly lower than that of tetracycline, but higher than that of P. aeruginosa. The antibacterial test results of essential oils from all three ginger varieties at concentrations of 62.6–1000 µg/mL proved to inhibit the growth of both test bacteria in the strong category, namely with an inhibition diameter > 20 mm. The strength of the antibacterial activity of essential oils against B. subtilis was lower than the standard antibiotic tetracycline, while against P. aeruginosa it was stronger. The essential oils of the three varieties have the potential to be used as antibacterial agents against gram-negative and gram-positive strains.
Pengaruh Air Kelapa Pada Media MS Terhadap Pertumbuhan Tunas Dari Eksplan Cormus Saffron (Crocus Sativus L.) Secara In vitro Utama, Rafii Satria; Setiari, Nintya; Nurchayati, Yulita; Haryanti, Sri
Buletin Anatomi dan Fisiologi Volume 11, Nomor 1, Tahun 2026
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.1.1.2026.1-8

Abstract

Saffron (Crocus sativus L.) merupakan tumbuhan steril dengan kromosom triploid, sehingga perbanyakan secara generatif tidak dapat dilakukan. Metode kultur jaringan menjadi salah satu alternatif untuk membudidayakan saffron. Tujuan penelitian adalah mengkaji pengaruh air kelapa pada media MS terhadap pertumbuhan tunas eksplan cormus Saffron  dan mengetahui konsentrasi air kelapa yang optimum sehingga meningkatan pertumbuhan tunas Saffron. Metode yaitu penanaman eksplan cormus Saffron  ke dalam media MS yang ditambah air kelapa pada konsentrasi 0%, 5%, 10%, 15%, 20%. Penelitian menggunakan rancangan acak lengkap dengan faktor tunggal (konsentrasi air kelapa) dengan 4 ulangan. Data dianalisis dengan ANOVA dilanjutkan dengan uji DMRT. Pertumbuhan eksplan diamati selama 8 minggu. Parameter yang diamati yaitu waktu muncul tunas, akar, dan daun; jumlah tunas, akar, dan daun. Hasil penelitian menunjukkan bahwa penambahan air kelapa tidak menginisiasi pertumbuhan akar, tunas, dan daun cormus saffron. Air kelapa dengan konsentrasi 0% - 20% tidak menstimulasi pertumbuhan akar, tunas, dan daun cormus saffron. Air kelapa belum mampu memecahkan dormansi cormus saffron.Saffron (Crocus sativus L.) is a sterile plant with triploid chromosomes, making generative propagation unfeasible. Tissue culture offers an alternative method for saffron cultivation. This research aimed to investigate the impact of coconut water in Murashige and Skoog (MS) medium on the growth of saffron corm explant shoots and to identify the optimal coconut water concentration for enhancing saffron shoot growth. The experiment involved planting saffron corm explants in MS medium supplemented with coconut water at concentrations of 0%, 5%, 10%, 15%, and 20%. A completely randomized design with a single factor (coconut water concentration) and four replications was employed. Data were analyzed using ANOVA followed by the Duncan's Multiple Range Test (DMRT). Explant growth was monitored over an 8-week period, with observations focusing on the timing of shoot, root, and leaf emergence, as well as the number of shoots, roots, and leaves. The results indicated that the addition of coconut water did not initiate the growth of saffron corm roots, shoots, or leaves. Coconut water at concentrations ranging from 0% to 20% did not stimulate the growth of saffron corm roots, shoots, or leaves, and it was unable to break the dormancy of saffron corms.

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