cover
Contact Name
Ilham
Contact Email
Ilham.fishaholic@gmail.com
Phone
+6221-64700928
Journal Mail Official
jra.puslitbangkan@gmail.com
Editorial Address
Gedung Balibang KP II, Lantai 2 Jl. Pasir Putih II, Ancol Timur, Jakarta Utara 14430
Location
Kab. jembrana,
Bali
INDONESIA
Jurnal Riset Akuakultur
ISSN : 19076754     EISSN : 25026534     DOI : http://doi.org/10.15578/JRA
Core Subject : Agriculture, Social,
Jurnal Riset Akuakultur as source of information in the form of the results of research and scientific review (review) in the field of various aquaculture disciplines include genetics and reproduction, biotechnology, nutrition and feed, fish health and the environment, and land resources in aquaculture
Arjuna Subject : Umum - Umum
Articles 7 Documents
Search results for , issue "Vol 15, No 3 (2020): (September, 2020)" : 7 Documents clear
DETEKSI PENYAKIT SCALE DROP PADA IKAN KAKAP PUTIH Lates calcarifer BLOCH Isti Koesharyani; Agus Sunarto; Ketut Sugama
Jurnal Riset Akuakultur Vol 15, No 3 (2020): (September, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.738 KB) | DOI: 10.15578/jra.15.3.2020.195-204

Abstract

Ikan kakap putih Lates calcarifer BLOCH sudah banyak dibudidayakan baik dalam bak di daratan ataupun dalam karamba jaring apung (KJA) di laut. Pada tahun 2010, terjadi kematian massal pada ikan kakap yang dipelihara dalam karamba jaring apung (KJA) di Batam dari ukuran 0,3 hingga 2 kg. Patogen penyebab kematiannya belum terkonfirmasi. Oleh karena itu, tujuan penelitian ini adalah untuk mendeteksi dan menentukan jenis patogen penyebab kematian ikan kakap putih yang dibudidayakan dalam KJA. Analisis deteksi dan penentuan jenis patogen dilakukan dengan metode polymerase chain reaction (PCR). Penyiapan sampel untuk analisis PCR diambil dari organ internal ikan kakap yang sakit, berupa limfa, ginjal, mata, dan otak yang diawetkan dalam larutan etanol 90%. Analisis PCR dilakukan menggunakan dua jenis primer spesifik megalocytivirus yaitu red sea bream iridovirus (RSIV) and scale drop disease (SDD). Hasil analisis menunjukkan adanya band spesifik pada 643 base pair (bp), yang berarti terkonfirmasi positif terinfeksi oleh SDD yang disebabkan oleh novel megalocytivirus dan ini merupakan kasus pertama terjadi di Indonesia, namun negatif terhadap infeksi RSIV. Analisis separasi susunan nukleotida melalui sekuensing, menunjukkan bahwa SDD dari sampel ikan kakap dari KJA Batam mirip 100% dengan SDDV yang berasal dari Singapura yang tersimpan di GenBank dengan nomor aksesi KRI.139659. Dari hasil penelitian ini terkonfirmasi bahwa virus scale drop disease adalah penyebab kematian massal ikan kakap dalam KJA di Batam. Untuk menghindari penyebaran virus SDD ke daerah lain di Indonesia disarankan untuk tidak menggunakan induk dan benih ikan kakap yang berasal dari Batam dan Singapura.Seabass, Lates calcarifer Bloch has been widely cultured in various land-based and marine farming systems. In 2010, widespread mass mortality of cultured seabass had occurred in floating fish cages in Batam coastal waters, particularly affecting the cultured fish sized between 0.3 and 2.0 kg. The pathogen suspected to cause the mortality has not yet been confirmed. The aim of this study was to determine and detect the pathogen that causes the fish mortality. Polymerase chain reaction (PCR) method was used in this research. The initial research stage involved the collection of the tissues of internal organs such as spleen, kidney, eye, and brain from the moribund fish and preserved in 90% ethanol solution. The PCR analysis was performed using two pair specific primers of megalocytivirus, red sea bream iridovirus (RSIV), and scale drop disease (SDD). The result of the analysis showed that the specific band appeared at 643 base pair (bp), confirming the positive infection of SDD belonged to the novel megalocytivirus. This research finding is the first report of the occurrence of novel megalocytivirus in Indonesia despite the negative detection of RSIV infection. The subsequent analysis of nucleotide structures by sequencing revealed that the Batam SDD had a 100% similarity to that of Singapore SDDV reported at GenBank with the accession number of KRI.139659. The present results confirmed that the pathogen infecting the cage culture of seabass in Batam is the SDD virus and caused high mortality rate. This research recommends prohibiting the use of broodstock and seed of seabass from either Batam or Singapore in order to avoid the spread of the SDD virus to other seabass mariculture areas in Indonesian.
POTENSI PREBIOTIK MADU KLENGKENG, RANDU, DAN ORGANIK TERHADAP KINERJA PERTUMBUHAN IKAN NILA (Oreochromis niloticus) Yani Aryati; Widanarni Widanarni; Dinamella Wahjuningrum; Iman Rusmana; Angela Mariana Lusiastuti
Jurnal Riset Akuakultur Vol 15, No 3 (2020): (September, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.738 KB) | DOI: 10.15578/jra.15.3.2020.185-193

Abstract

Salah satu upaya untuk meningkatkan performa pertumbuhan dan komposisi mikroflora dalam usus, menghambat pertumbuhan patogen, dan meningkatkan imunitas ikan adalah dengan pemberian prebiotik. Madu memiliki kandungan utama berupa oligosakarida, salah satu material yang dapat dimanfaatkan sebagai prebiotik. Penelitian ini bertujuan untuk mengevaluasi potensi madu klengkeng, randu, dan organik sebagai prebiotik dan menguji pengaruhnya terhadap kinerja pertumbuhan ikan nila (Oreochromis niloticus). Kriteria prebiotik yang diuji meliputi kandungan oligosakarida dari madu dan ekstraknya menggunakan kromatografi lapis tipis (KLT), hidrolisis asam lambung dan a-amilase dari ketiga ekstrak madu, serta stimulasi pertumbuhan bakteri probiotik dari ekstrak ketiga jenis madu. Pengujian pada ikan nila dilakukan selama 30 hari dengan perlakuan A (kontrol), B (1% madu klengkeng pada pakan), C (1% madu randu pada pakan), dan D (1% madu organik pada pakan), masing-masing diulang empat kali. Parameter kinerja pertumbuhan yang diukur meliputi bobot biomassa awal, bobot biomassa akhir, pertambahan bobot, konsumsi pakan, laju pertumbuhan spesifik, rasio konversi pakan, dan sintasan. Hasil pengujian dengan KLT menunjukkan ekstrak madu klengkeng dan randu merupakan oligosakarida. Hidrolisis asam lambung dan asam á-amilase selama tiga jam pengamatan mengalami peningkatan, baik dari ekstrak madu klengkeng, madu randu, dan madu organik. Ekstrak madu randu memiliki kinerja paling baik dalam menstimulasi pertumbuhan bakteri probiotik. Madu klengkeng, randu, dan organik memenuhi kriteria prebiotik. Penambahan madu randu pada pakan ikan nila mampu meningkatkan bobot biomassa akhir tertinggi (28,42 ± 0,79); laju pertumbuhan spesifik tertinggi (11,15 ± 0,09), dan memberikan rasio konversi pakan terendah (1,77 ± 0,06) dibandingkan dengan perlakuan madu yang lain. Penambahan madu klengkeng, randu, dan organik sebanyak 1% melalui pakan mampu meningkatkan kinerja pertumbuhan ikan nila dengan hasil terbaik adalah penambahan madu randu.One of the efforts to improvethe growth performance and composition of microflora in the intestine, inhibit the growth of pathogens, and increase the immunity of fish is through the application of prebiotics. Honey is known to contain oligosaccharides, which can be used as prebiotics. This study aimed to evaluate the potential of longan, kapok, and organic honey as prebiotics and examine their effects on the growth performance of tilapia (Oreochromis niloticus). The prebiotic criteria tested included oligosaccharide content from the honey and their extracts using thin layer chromatography (TLC), gastric acid hydrolysis, and a-amylase as well as stimulation of growth of probiotic bacteria from the extract of the honey. Tests on tilapia were carried out for 30 days with treatments A (control), B (1% longan honey added in the feed), C (1% kapok honey added in the feed), and D (1% organic honey added in the feed) and each treatment repeated four times. The measured growth performance parameters included initial biomass weight, final biomass weight, weight gain, feed consumption, specific growth rate, feed conversion ratio, and survival. Test results with TLC showed that the extract of longan honey and kapok were oligosaccharides. Gastric acid hydrolysis and á-amylase acid during three hours of observation showed an increase, both from the extract of longan, kapok, and organic honey. The extract of kapok honey has the best performance in stimulating the growth of probiotic bacteria. Longan, kapok, and organic honey meet the criteria required for prebiotic. The addition of kapok honey intilapia feed attained the highest final biomass weight (28.42 ± 0.79), the highest specific growth rate (11.15 ± 0.09), and produced the lowest feed conversion ratio (1.77 ± 0.06) compared to the other honey treatments. The addition of 1% longan, kapok, and organic honey in feed can improve the growth performance of tilapia, of which kapok honey gives the best result. 
EMBRIOGENESIS DAN PERKEMBANGAN LARVA SIPUT GONGGONG, Laevistrombus turturella PADA SUHU INKUBASI BERBEDA Muzahar Muzahar; Muhammad Zairin Jr.; Fredinan Yulianda; Muhammad Agus Suprayudi; Alimuddin Alimuddin; Irzal Effendi
Jurnal Riset Akuakultur Vol 15, No 3 (2020): (September, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.738 KB) | DOI: 10.15578/jra.15.3.2020.159-164

Abstract

Fase larva pada siput gonggong sebagaimana pada biota akuatik lain adalah fase yang peka dan rawan kematian. Penyerapan kuning telur untuk pembentukan organ dalam terjadi pada fase ini. Suhu air memengaruhi perkembangan embrionik dan metabolisme dalam tubuh biota. Informasi tentang pengaruh suhu inkubasi terhadap embriogenesis dan perkembangan larva siput gonggong masih terbatas. Penelitian ini bertujuan untuk menentukan pengaruh suhu berbeda terhadap embriogenesis dan perkembangan larva siput gonggong. Percobaan menggunakan rancangan acak lengkap dengan tiga perlakuan dan dua ulangan. Tiga perlakuan perbedaan suhu yang diberikan yaitu 27°C, 29°C, dan 31°C. Sampel telur yang digunakan berasal dari hasil pemijahan semibuatan. Hasil penelitian menunjukkan bahwa inkubasi pada suhu air 31°C memberikan stimulasi tercepat pada embriogenesis dan perkembangan larva siput gonggong daripada perlakuan lainnya. Larva siput gonggong menetas dan berenang bebas pada jam ke-94 pasca-inkubasi. Penelitian dengan perlakuan yang sama perlu dilanjutkan untuk dapat menghasilkan benih siput gonggong.Similar to most of the other aquatic biota, the larval phase of gonggong conch is considered a sensitive and death-prone life stage. The absorption of egg yolk to form the internal organs occurs in this phase. Certain external factors, particularly water temperature, play a significant influence on the embryonic development and metabolic processes of gonggong conch larvae. However, the extent of the effects of incubation temperature on the embryogenesis and larval development of gonggong conch has not been determined or thoroughly studied. This study aimed to determine the effects of different temperatures on the embryogenesis and larval development of gonggong conch. The experiment used a completely randomized design with three treatments and two replicates. The temperature treatments were 27°C, 29°C, and 31°C. The egg samples used were collected from the semi-artificial spawnings of gonggong conch. The results showed that the egg incubation using the water temperature of 31°C provided the fastest stimulation in the embryogenesis and development of gonggong conch larvae than the other treatments. Gonggong conch larvae hatched and swam freely in the 94th hours post-incubation. Research with the same treatment needs to be continued to be able to produce gonggong conch seeds.
RESPONS FISIOLOGIS DAN KINERJA PERTUMBUHAN IKAN NILA PADA MEDIA RENDAH AMONIA DAN DIBERI SUPLEMEN ASAM GLUTAMAT Titin Kurniasih; Dedi Jusadi; Muhammad Agus Suprayudi; Sri Nuryati; Muhammad Zairin Jr.; Eddy Supriyono
Jurnal Riset Akuakultur Vol 15, No 3 (2020): (September, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.117 KB) | DOI: 10.15578/jra.15.3.2020.175-183

Abstract

Ketika dipapar media tinggi amonia, ikan nila mengalami perubahan metabolisme asam amino yang cukup signifikan, dan suplementasi asam glutamat berguna untuk memperbaiki perubahan yang merugikan akibat paparan amonia. Akan tetapi informasi mengenai aspek metabolisme asam amino pada ikan nila yang dipapar amonia rendah masih sangat terbatas. Penelitian ini dilakukan untuk mengevaluasi pengaruh suplementasi asam glutamat pada ikan nila merah yang dipelihara pada media budidaya rendah amonia terhadap respons fisiologis dan kinerja pertumbuhan. Ikan nila dengan bobot rata-rata 9,97 ± 0,38 g ditebar sebanyak 20 ekor pada setiap akuarium (padat tebar 1,0 g L-1). Empat jenis pakan isoprotein (kadar protein 28%) dan isoenergi (4245 ± 22,48 kkal kg-1) disuplementasi asam glutamat masing-masing sebanyak 0% (Glu 0), 0,75% (Glu 0,75), 1,5% (Glu 1,5) dan 2,25% (Glu 2,25). Setiap perlakuan diberi empat ulangan. Penelitian ini dilakukan selama 60 hari. Hasil penelitian menunjukkan bahwa pemberian pakan yang ditambah glutamat memberi efek pada respon fisiologis ikan. Aktivitas enzim aspartate aminotransferase (AST) pada Glu 2,25 lebih rendah dibandingkan dengan perlakuan lainnya, yang menjadi indikasi penurunan beban kerja hati. Ada kecenderungan peningkatan kadar aspartat, alanin, leusin, isoleusin dan valin pada jaringan hati seiring dengan meningkatnya kadar suplementasi asam glutamat. Di dalam penelitian ini, kinerja pertumbuhan ikan tidak berbeda nyata antar perlakuan. Kesimpulannya adalah bahwa konsumsi pakan yang ditambah asam glutamat 2,25% mampu memperbaiki respons fisiologis ikan akibat menurunnya beban kerja hati yang dicirikan dengan penurunan nilai AST, serta meningkatnya kandungan beberapa asam amino hati, walau belum mampu memperbaiki kinerja pertumbuhan dan pemanfaatan pakan oleh ikan nila. Informasi ini berguna untuk pengembangan riset terkait aspek metabolisme asam amino pada ikan nila yang terpapar media tinggi amonia.When exposed to high ammonia aquatic environment, nile tilapia experienced a significant change in hepatic amino acid metabolism and glutamic acid supplementation can reduce the effects of the adverse change. However, there are no sufficient information on the amino acid metabolisme of tilapia exposed to low environmental ammonia. This research was performed to evaluate the effects of oral supplementation of glutamic acid on the aminotransferase enzymes activity and growth performance of red tilapia reared in low environmental ammonia (LEA) with NH4 concentration of 0.10 mg L-1. Fish with an average weight of 9.97 ± 0.38 g were stocked with an initial rearing density of 1.0 g L-1(20 fish in each aquarium). Four isonitrogenous (crude protein 28%) and isocaloric (4246 ± 22.48kcal kg-1) experimental diets were prepared with supplementation of different ratios of glutamic acid at 0% (Glu0), 0.75% (Glu0.75), 1.5%(Glu1.5) and 2.25 % (Glu2.25) to feed, respectively. All treatment groups were arranged quadruplicate. Fish were fed with the diets for 60 days. The results showed that the supplementation of glutamic acid in the diet affected the physiological response of the fish. The aspartate aminotransferase (AST) activity of Glu2.25 was significantly lower compared to that of the other treatments, which indicated a decrease in liver workload. There is a tendency of increased levels of hepatic free aspartate, alanine, leucine, isoleucine, and valine following the increase of glutamic acid supplementation level. The fish growth performance was insignificantly different between the treatments. It is concluded that a diet supplemented with 2.25% of glutamic acid could improve the physiological response of red tilapia, although no significant growth improvement should be expected. These research finding could serve as an important basic information for future research on amino acid and endogenous ammonia metabolism in nile tilapia exposed to high ammonia aquatic environment.
PENGARUH PERLAKUAN SUHU MEDIA BERBEDA TERHADAP VIABILITAS DAN PENETASAN TELUR IKAN TUNA SIRIP KUNING Thunnus albacares (Bonnaterre, 1788) Ananto Setiadi; Jhon Harianto Hutapea; Gunawan Gunawan
Jurnal Riset Akuakultur Vol 15, No 3 (2020): (September, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.738 KB) | DOI: 10.15578/jra.15.3.2020.151-158

Abstract

Sejak tahun 2015 penelitian pembesaran larva ikan tuna sirip kuning (Thunnus albacares) hasil pemijahan di keramba jaring apung (KJA) berhasil memproduksi juvenil 500-1.000 ekor benih per percobaan. Permasalahan utama adalah daya tetas telur yang berfluktuasi karena suhu air media inkubasi tidak stabil. Penelitian bertujuan untuk menentukan suhu air yang optimum untuk mendapatkan daya tetas telur yang tinggi. Penelitian ini menggunakan sembilan bak fiberglass transparan berbentuk kerucut dengan volume 200 L diisi dengan 50.000 butir telur/bak pada stadia morula. Penelitian ini menggunakan rancangan acak lengkap dengan perlakuan suhu media awal ditambah kisaran yang berbeda yaitu (A) 28,7 ± 0,5°C; (B) 28,7 ± 1,0°C; dan (C) 28,7 ± 2,0°C; masing masing perlakuan dengan tiga kali ulangan. Hasil penelitian menunjukkan bahwa daya tetas telur tertinggi (42.500 ekor atau 85,3 ± 9,2%) pada perlakuan A dan berbeda nyata (P<0,05) dengan perlakuan B (22.500 ekor atau 44,6 ± 16,3%) dan C (12.000 ekor atau 24,0 ± 2,6%). Ukuran larva yang baru menetas pada perlakuan A, panjang badannya lebih panjang dan berbeda nyata (P<0,05) dibandingkan dengan perlakuan C. Peningkatan suhu menyebabkan peningkatan pemanfaatan kuning telur. Laju pemanfaatan kuning telur pada perlakuan (C) 28,7 ± 2,0°C mencapai 0,02 mm3 jam-1 dua kali lebih tinggi dibandingkan pada perlakuan (A) suhu 28,7 ± 0,5°C yang hanya 0,01 mm3 jam-1. Semakin tinggi peningkatan suhu inkubasi dari suhu awal, mortalitas embrio semakin tinggi (P<0,05) sehingga daya tetas telur ikan tuna berbeda nyata antar perlakuan. Direkomendasikan bahwa peningkatan optimum suhu untuk menstimulasi daya tetas telur ikan tuna sirip kuning yang lebih tinggi adalah 0,5°C dari suhu sekitar.Since 2015, researchers from the Institute for Mariculture Research and Fisheries Extension, Gondol have successfully carried out larval rearing of yellowfin tuna (Thunnus albacares) produced from broodstocks spawning in floating net cages. The number of produced yellowfin tuna juveniles ranged between 500-1,000 juveniles per batch. One of the main problems in yellowfin tuna larval rearing is related to the fluctuation of eggs hatching rate due to unstable water incubation temperatures. The aim of the research was to determine the optimum temperature to obtain a maximum egg hatching rate. In this research, nine transparent cone-shaped fiberglass containers with a volume of 200 L were used and stocked with 50,000 eggs morula stage/tank. The experiment was arranged in a completely randomized design with the treatments described in the following. The ambient hatchery water temperature was 28.7°C and was increased at specific increment temperature settings as the treatments as follows: (A) 28.7 ± 0.5°C; (B) 28.7 ± 1.0°C; and (C) 28.7 ± 2.0°C. Each temperature treatment had three replicates. The results showed that the highest hatching rate of eggs (42,500 larvae or 85.3 ± 9.2%) was observed in treatment A. The result from treatment A was significantly different (P<0.05) than that of treatment B (22,500 larvae or 44.6 ± 16.3% and C (12,000 larvae or 24.0 ± 2.6%). The hatched larvae in treatments A has longer body length and was significantly different (P<0.05) compared to treatment C. The increase of temperature in each treatment has led to a rise in the yolk utilization rate. The yolk utilization rate in treatment (C) of 28.7 ± 2.0°C was 0.02 mm3 h-1, two times higher compared to treatment (A) 28.7 ± 0,5°C, which was only 0.01 mm3 h-1. The higher the incubation temperature, the higher the embryo mortality (P<0.05). Therefore, the hatching rate of tuna eggs was significantly different among the treatments. This research recommends that the optimum stimulating temperature increase to improve the hatching rate of yellowfin tuna eggs is 0.5°C from the ambient temperature.
KERAGAMAN GENETIK DAN BIOREPRODUKSI EMPAT POPULASI IKAN MATA MERAH (Puntius orphoides Valenciennes, 1842) Wahyulia Cahyanti; Fera Permata Putri; Sri Sundari; Anang Hari Kristanto
Jurnal Riset Akuakultur Vol 15, No 3 (2020): (September, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.738 KB) | DOI: 10.15578/jra.15.3.2020.141-149

Abstract

Ikan mata merah merupakan ikan konsumsi yang berkerabat dekat dengan ikan tawes yang terdistribusi luas di kawasan tropis. Analisis keragaman genetik dan performa reproduksi ikan mata merah, baik jantan maupun betina belum tersedia secara lengkap dan valid. Tujuan penelitian ini adalah untuk menentukan keragaman genetik ikan mata merah dari berbagai lokasi, dan mengkaji bioreproduksinya. Ikan sampel yang diperoleh dari nelayan dan pengumpul di empat lokasi berbeda yaitu: Jawa Barat (Tasikmalaya dan Cianjur), Jawa Tengah (Purwokerto), dan Jawa Timur (Umbulan, Pasuruan), ditampung di Balai Benih Ikan lokal, kemudian ditransportasikan ke Instalasi Plasma Nutfah, Cijeruk, Bogor. Sampel ikan dari berbagai ukuran digunakan untuk analisis keragaman genetik melalui analisis morfometrik dan RAPD, sedangkan untuk kajian bioreproduksi ikan yang digunakan adalah calon dan induk ikan mata merah jantan dan betina. Hasil analisis menunjukkan bahwa keragaman genetik populasi Purwokerto dan Umbulan memiliki perbedaan dengan nilai sharing component intraspesies tertinggi, yaitu 100,0%. Polimorfisme empat populasi ikan mata merah berkisar antara 1,92%-17,30% dengan kisaran tingkat heterozigositas 0,0088-0,0678. Populasi Purwokerto memiliki jarak genetik terjauh (0,0678) dari populasi lainnya. Pada pengamatan kinerja reproduksi hanya dua populasi yang matang gonad dan bisa dipijahkan, yaitu populasi Tasikmalaya dari 10 ekor matang gonad, tiga ekor berhasil memijah, dan Cianjur dari sembilan ekor matang gonad, tiga ekor berhasil memijah dengan nilai derajat pembuahan kedua populasi sebesar 100% dan derajat penetasan yang masih cukup tinggi Tasikmalaya 84,32 ± 7,38a% dan Cianjur 73,15 ± 3,78a%. Volume sperma ikan jantan diperoleh sebanyak 0,1 mL; dengan jumlah spermatozoa sebanyak 75 x 108 sel.Red-eye fish is a fish species closely related to tawes/silver barb/Java barb and widely distributed in tropical regions. As of now, information on genetic diversity and reproductive performance of red-eye fish, both male and female, have not yet been completed. Thus, this study was conducted to determine the genetic diversity of red-eye fish populations from different locations and study their bioreproduction. Live fish were obtained from fishermen and collectors in four different areas namely West Java (Tasikmalaya and Cianjur), Central Java (Purwokerto), and East Java (Umbulan, Pasuruan). The collected fish were temporarily stored in each local fish seed center before transported to the Germplasm Installation, Cijeruk, Bogor. Fish samples of various sizes were used for genetic diversity analysis through morphometric analysis and RAPD. In bioreproduction observation, the fish used were prospectively matured and matured male and female of red-eye fish. The results of the genetic study showed that the Purwokerto and Umbulan populations have genetic diversity differences with the highest intraspecies sharing component value of 100.0%. The polymorphism of four red-eye fish populations ranged from 1.92%-17.30% with a range of heterozygosity levels of 0.0088-0.0678. Purwokerto population has the farthest genetic distance (0.0678) from the other populations. The observation of reproductive performance found only two populations having mature gonad fish and could subsequently be artificially spawned. The populations were Tasikmalaya population with ten mature gonad fish, of which three fish successfully spawned and Cianjur population with nine mature gonad fish, of which three successfully spawned. The fertilization rate of the two spawned populations was 100%. The hatching rateof eggs from Tasikmalaya and Cianjur populations were relatively high of 84.32 ± 7.38a% and 73.15 ± 3.78a%, respectively. The maximum volume of sperm produced by each male fish from both populations was 0.1 mL, with the total number of spermatozoa of 75-108 cells.
PEMANFAATAN PERIFITON PADA JUMLAH SUBSTRAT BERBEDA TERHADAP KUALITAS AIR DAN KINERJA PERTUMBUHAN BENIH IKAN GURAME (Osphronemus gouramy) Atma Jaya Salman Muin; Kukuh Nirmala; Mia Setiawati; Yuni Puji Hastuti
Jurnal Riset Akuakultur Vol 15, No 3 (2020): (September, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.738 KB) | DOI: 10.15578/jra.15.3.2020.165-173

Abstract

Tujuan penelitian ini adalah untuk menganalisis pemanfaatan jumlah substrat tali rafia yang berisi perifiton dalam meningkatkan kualitas air media pemeliharaan untuk pertumbuhan benih ikan gurame (Osphronemus gouramy). Perlakuan terdiri dari penambahan A (0 substrat), B (1 substrat), C (2 substrat), dan (3 substrat) tali rafia berisi perifiton (bobot 5 g/substrat). Benih ikan gurame 32 ekor (panjang total 4,8±0,30 cm dan bobot 1,9±0,38 g/ekor) dipelihara pada akuarium ukuran 27 cm x 30 cm x 60 cm. Selama pemeliharaan benih ikan gurame diberi pakan komersil secara at satiation tiga kali sehari. Parameter yang diamati adalah kualitas air, laju pertumbuhan spesifk, tingkat kelangsungan hidup, dan rasio konversi pakan. Hasil analisis kualitas air diperoleh nilai kisaran optimum untuk pertumbuhan benih ikan gurami pada perlakuan C (2 substrat) dan perlakuan D (3 substrat), Hasil penelitian menunjukkan bahwa terdapat pengaruh nyata (P<0,05) pada penambahan substrat yang berbeda terhadap kinerja pertumbuhan benih ikan gurame. Pada perlakuan C (2 substrat) memiliki performa pertumbuhan terbaik yaitu laju pertumbuhan spesifik panjang (cm) 1,33±0,03, laju pertumbuhan spesifik bobot (g) 4,03±0,12, tingkat kelangsungan hidup (%) 86,46±1,04, dan rasio konversi pakan 0,81±0,01. Substrat tali rafia dapat dimanfaatkan sebagai media menempelnya perifiton, pemanfaatan 2 substrat tali rafia (10 gr) dapat diperoleh beberapa kelas perifiton yang dapat dijadikan sumber pakan alami untuk meningkatkan performa pertumbuhan benih ikan gurame dan dapat mempertahankan kualitas air media pemeliharaan. Perlu dilakukan kajian lebih lanjut mengenai pemanfaatan jenis substrat lainnya untuk meningkatkan produksi perifiton agar mengurangi penggunaan pakan komersil.The purpose of this study was to determine the optimum number of filled-periphyton raffia substrates serving as natural food to improve the growth performance of gouramy seeds (Osphronemus gouramy) and maintain the water quality rearing media. The treatments consisted of the addition of A (0 substrate), B (1 substrate), C (2 substrates), and D (3 substrates) raffia rope substrates filled with periphyton (5 g periphyton/substrate). Gouramy seeds of 32 individuals (total length 4.8±0.30 cm dan weight 1.9±0.38 g/ind.) were reared in an aquarium measuring 37 cm x 30 cm x 60 cm in size. During the rearing period, the gouramy seeds were fed with a commercial diet at satiation three times a day. The parameters observed were water quality parameters, specific growth rate, survival rate, and food conversion ratio. The results showed that treatment C (2 substrates) and D (3 substrates) had the optimum ranges of water quality to support the growth of gourami seeds. The statistical analysis also confirmed a significant (P<0.05) effect of the addition of different substrates to the growth performance of gouramy seeds. Gouramy seeds in treatment C (2 substrates) had the best growth performance in terms of specific length growth rate (cm) 1.33±0.03, specific weights growth rate (g) 4.03±0.12, survival rate (%) 86.46±1.04, and food conversion ratio 0.81±0.01. The raffia rope substrate can be used as a medium for attaching periphyton. The use of 2 raffia rope substrates (10 g) can be obtained from several classes of periphyton which can be used as a natural food source to improve the growth performance of gourami seeds and maintain the water quality of the maintenance media. It is necessary to carry out further studies regarding the use of other types of substrates to increase periphyton production in order to reduce the use of commercial feeds.

Page 1 of 1 | Total Record : 7


Filter by Year

2020 2020


Filter By Issues
All Issue Vol 20, No 4 (2025): Desember (2025) Vol 20, No 3 (2025): September (2025) Vol 20, No 2 (2025): Juni (2025) Vol 20, No 1 (2025): Maret (2025) Vol 19, No 4 (2024): Desember (2024) Vol 19, No 3 (2024): September (2024) Vol 19, No 2 (2024): Juni (2024) Vol 19, No 1 (2024): (Maret 2024) Vol 18, No 4 (2023): (Desember, 2023) Vol 18, No 3 (2023): (September, 2023) Vol 18, No 2 (2023): (Juni, 2023) Vol 18, No 1 (2023): (Maret 2023) Vol 17, No 4 (2022): (Desember 2022) Vol 17, No 3 (2022): (September) 2022 Vol 17, No 2 (2022): (Juni) 2022 Vol 17, No 1 (2022): (Maret, 2022) Vol 16, No 4 (2021): (Desember, 2021) Vol 16, No 3 (2021): (September, 2021) Vol 16, No 2 (2021): (Juni, 2021) Vol 16, No 1 (2021): (Maret, 2021) Vol 15, No 4 (2020): (Desember, 2020) Vol 15, No 3 (2020): (September, 2020) Vol 15, No 2 (2020): (Juni, 2020) Vol 15, No 1 (2020): (Maret, 2020) Vol 14, No 4 (2019): (Desember, 2019) Vol 14, No 3 (2019): (September, 2019) Vol 14, No 2 (2019): (Juni, 2019) Vol 14, No 1 (2019): (Maret, 2019) Vol 13, No 4 (2018): (Desember 2018) Vol 13, No 3 (2018): (September 2018) Vol 13, No 2 (2018): (Juni, 2018) Vol 13, No 1 (2018): (Maret 2018) Vol 12, No 3 (2017): (September 2017) Vol 12, No 4 (2017): (Desember 2017) Vol 12, No 2 (2017): (Juni 2017) Vol 12, No 1 (2017): (Maret 2017) Vol 11, No 3 (2016): (September 2016) Vol 11, No 4 (2016): (Desember 2016) Vol 11, No 2 (2016): (Juni 2016) Vol 11, No 1 (2016): (Maret 2016) Vol 8, No 3 (2013): (Desember 2013) Vol 5, No 3 (2010): (Desember 2010) Vol 5, No 2 (2010): (Agustus 2010) Vol 5, No 1 (2010): (April 2010) Vol 2, No 2 (2007): (Agustus 2007) Vol 2, No 1 (2007): (April 2007) Vol 1, No 1 (2006): (April 2006) Vol 10, No 4 (2015): (Desember 2015) Vol 10, No 3 (2015): (September 2015) Vol 10, No 2 (2015): (Juni 2015) Vol 10, No 1 (2015): (Maret 2015) Vol 9, No 3 (2014): (Desember 2014) Vol 9, No 2 (2014): (Agustus 2014) Vol 9, No 1 (2014): (April 2014) Vol 8, No 2 (2013): (Agustus 2013) Vol 8, No 1 (2013): (April 2013) Vol 7, No 3 (2012): (Desember 2012) Vol 7, No 2 (2012): (Agustus 2012) Vol 7, No 1 (2012): (April 2012) Vol 6, No 3 (2011): (Desember 2011) Vol 6, No 2 (2011): (Agustus 2011) Vol 6, No 1 (2011): (April 2011) Vol 4, No 3 (2009): (Desember 2009) Vol 4, No 2 (2009): (Agustus 2009) Vol 4, No 1 (2009): (April 2009) Vol 3, No 3 (2008): (Desember 2008) Vol 3, No 2 (2008): (Agustus 2008) Vol 3, No 1 (2008): (April 2008) Vol 2, No 3 (2007): (Desember 2007) Vol 1, No 3 (2006): (Desember 2006) Vol 1, No 2 (2006): (Agustus 2006) More Issue