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INDONESIA
Majalah Kedokteran Andalas
Published by Universitas Andalas
ISSN : 01262092     EISSN : 24425230     DOI : https://doi.org/10.25077
Core Subject : Health,
Health Professions Medicine & Pharmacology Public Health
Majalah Kedokteran Andalas (MKA) (p-ISSN: 0126-2092, e-ISSN: 2442-5230) is a peer-reviewed, open-access national journal published by Faculty of Medicine, Universitas Andalas and is dedicated to publish and disseminate research articles, literature reviews, and case reports, in the field of medicine and health, and other related disciplines
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 15 Documents
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Search results for , issue "Vol 37 (2014): Supplement 2 | Published in December 2014" : 15 Documents clear
HUBUNGAN KADAR BETA.SITE APP.CLEAVING ENZYME 1, BETA-AMYLOID DAN 4 HYDROXINONENAL PLASMA DENGAN GANGGUAN FUNGSI KOGNITIF PADA PENDERITA PASCASTROKE ISKEMIK Yuliarni Syafrita; Yanwirasti Yanwirasti; M Hasan Machfoed; Darwin Amir
Majalah Kedokteran Andalas Vol 37 (2014): Supplement 2 | Published in December 2014
Publisher : Faculty of Medicine, Universitas Andalas

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Abstract

Background: The levet of beta-amyloid (Ap) in the cerebrospinal fluid (CSS) can beused asa marker to detect cognitive impairment, but fhe CSS retrieval technique is invasive. so if r'snecessa4z fo find biomarkers that are relatively easy, cheap and reliable. Therefore, biomarkersthat can be measured in blood is needed.Aims: To determine fhe association of the blood levels of BACE-I, AB and 4HNE with ischemiccognitive functisn after stroke event.Methads: This study was an observational study with cross sectional design using cases andcontrols. A number af 84 patients with ischemic stroke and 42 normal subjecfs as controls wereenrolled. Cognitive function u/as assessed 3 months aftdr stroke event using MoCA-lna testand measurement of blood levels of BACE1-1, 4p40, AP42 and 4HNE was conducted within72 hours of onset of stroke. Regression statistical analysis was used to determine the mostdaminant factars related to the occurrence of impaired cognitive function after stroke event.Resuffs; ln bivariate analysis, we found a significant assocrafion between cognitive impairmentafter stroke with high blood levels of BACE1 (p = 0.004, OR = 1.714), low levels of AB40 (p =A.0001, AR = 14.80) and low /eve/s of AB42 @ = A.U7, OR = 3.44). There was no significantassociation between the blood levels of 4HNE with impaired cognitive function after strokeevent. ln multivariate analysis, we found low low plasma level of 4840, high level of BACE-1,and low level of AB42 were variables strongly related with cognitive impairment after ischemicstroke subsequently based on the strength of correlation.Canclusians: Low levels of 4840, ttigh levels of BACE-I and low ievels of Ap42 are associatedwith the incidence of impaired cagnitive functian after ischemic stroke.M}(A, Volume 37, Nomor.Supl. 2, November 2014MKA, Volume 37, Nomor.Supl. 2, November 2014 http:/imka.fk. u nand.ac. id/ABSTRAKLatar Belakang : Kecacatan pascastroke diperberat oleh terganggunya fungsi kognitif. Metodediagnostik yang tersedia sekarang, tidak cukup sensitif bila digunakan sebagai upaya untukmenemukan gangguan kognitif pascastroke secara dini. Kadar beta-amyloid (AP) di cairanserebrospinal (CSS) dapat dijadikan sebagai penanda telah terjadinya gangguan kognitif,namun teknik pengambilan CSS adalah invasive, sehingga perlu dicari biomarker yang relatifmudah, murah dan dapat dipercaya. Untuk itu perlu dicarai biomarker pada plasma.Tujuan : Untuk mengetahui hubungan kadar BACE-1, AB dan 4HNE plasma dengan fungsikognitif pascastroke iskemik.Metode : Penelitian ini bersifat observasional dengan disain cross sectional, menggunakankasus dan kontrol. Mengikutkan 84 penderita pascastroke iskemik dan 42 subjek normalsebagai kontrol. Fungsi kognitif diperiksa 3 bulan pascastroke dengan test MoCA-lna danpemeriksaan kadar BACEI-1, AF40, AP42 dan 4HNE plasma dalam 72 jam onset stroke.. Analisa regresi statitistik digunakan untuk menetapkan faktor mana yang paling dominanhubungannya dengan terjadinya gangguan fungsi kognitif pascastroke iskemik.Hasil : Pada analisis bivariat, ditemukan hubungan yang bermakna antara gangguan kognitifpascastroke dengan tingginya kadar BACE1 (p=0,004, OR= 4,714), rendahnya kadar AP40(p=0,0001, OR=14,80) dan rendahnya kadar AP42 (p=0,0t7, OR=3,44). Tidak ditemukanhubungan yang bermakna antara kadar 4HNE dengan gangguan fungsi kognitif pascastroke.Pada analisis multivariat, didapatkan rendahnya kadar AB40 dan tingginya kadar BACE-1 plasma sebagai variabel yang kuat hubungannya dengan kejadian gangguan kognitifpascastroke iskemik.Kesimpulan : Rendahnya kadar AB40 dan tingginya kadar BACE-1 plasma berhubungandengan kejadian gangguan fungsi kognitif pascastroke iskemik.62
Migration assay on primary culture isolated from patient's primary breast cancer tissue ED Yuliana; A Bustami A Bustami; HP Tantry
Majalah Kedokteran Andalas Vol 37 (2014): Supplement 2 | Published in December 2014
Publisher : Faculty of Medicine, Universitas Andalas

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Abstract

Background: Migration is an essential component of breast cancer metastasis, which studyhas been concentrated on culture of established breast cancer cell lines that do not accuratelyrepresent the sophistication and heterogeneity of patient's breast cancer. An attempt toperform migration assay using Boyden Chamber Assay (BCA) on primary culture originatingfrom patient's breast cancer tissue was developed to accommodate upcoming study of breastcancer migration in lndonesian patients.Methods: Pathologically proven primary breast cancer tissue samples were obtained fromCiptomangunkusumo Hospital during core (n=4) and incisional (n=3) biopsies of stage llAup to stage lllA breast cancer patients. Following biopsy, the breast cancer tissue samplesunderwent processings to isolate the cancer cells. These cancer cells were -then resuspendedwithin Dulbecco's modified Eagle's medium (DMEM) ahd cultured in 12-well plate. The growthof primary culture were observed and compared between the core biopsy and the incisionalbiopsy specimens. Optimization of BCA method was later performed to investigate themigration of the breast cancer primary culture towards different experirnental conditions, whichwere control, Fetal Bovine Serum (FBS), and Stromal Derived Factor-l (SDF-1). Two differentnumber of breast cancer cells were tested for the optimization of the BCA, which were 1 x 105and3x105cells.Results: None of the culture performed on core biopsy specimens grew, while one out ofthree incisional biopsy specimens grew until confluence. The one primary culture that grewwas later assesed using BCA to assess its migration index towards different experimentalconditions. Using 1 x 10s breast cancer cells in the BCA , the result of the absorbance level ofmigrated cells showed that the migration towards SDF-1 (0.529) nearly doubled the migrationtowards controlmedium (0.239) and FBS (0.209). Meanwhile, the absorbance levelwas simiiarbetween the control medium (1.050), FBS (1 .103), and SDF-1 (1 .104) when the same test wasrun with 3 x 105 breast cancer cells.Discussion: Breast cancer tissue collected using incisional biopsies hao better chance togrow in primary culture than those collected using core biopsies, possibly due to the differencein the amount of tissue extracted. Consistent with previous research, patient's breast cancercells was shown to migrate more towards the chemokine ligand SDF-1, as compared to controlmedium and FBS. The number of breast cancer cells run in the BCA also affected the result ofthe migration assay, where 1 x 10s cells showed a superiority as compared to 3 x 105 cells inshowing the migration difference between the different experimental conditions.MKA, Volume 37, Nomor.Supl. 2, November 2014ED Yulian dkk"Migration assay on primary culture isolated from patient's primary breast cancer tissueConclusions: This study encourages and facilitates future research to study migration the intrinsic characteristic of individualized patient's breast cancer as well as to assess the change in migration characteristic of breast cancer cells after an in vivo treatment in human.
Metilasi DNA dan Peranannya Pada Kanker Payudara Sporadik Wirsma Arif Harahap
Majalah Kedokteran Andalas Vol 37 (2014): Supplement 2 | Published in December 2014
Publisher : Faculty of Medicine, Universitas Andalas

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Abstract

Metilasi DNA dan Peranannya Pada Kanker Payudara Sporadik
Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasa Desmawati Desmawati
Majalah Kedokteran Andalas Vol 37 (2014): Supplement 2 | Published in December 2014
Publisher : Faculty of Medicine, Universitas Andalas

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Abstract

Prevalensiobesitas makin lama makin meningkat. Obesitas berhubungan dengan peningkatankadar angiotensinogen (AGT) plasma dan tekanan darah. Salah satu pengukuran statusgizi dengan antropometri. Penelitian ini bertujuan untuk mengetahui korelasi pengukuranantropometri dengan tekanan darah dan kadar AGT plasma pada orang dewasa etnikMinangkabau.Sebanyak 75 subyek berusia 35 - 54 tahun di Padang diambil secara random. Seluruh subyekdiwawacara untuk mengetahui karakteristik dan tingkat aktivitas fisik. Pengukuran persentaselemak tubuh dan antropometri ( indeks massa tubuh /lMT dan lingkar pinggang / LP), tekanandarah dan pemriksaan kadar AGT plasma juga dilakukan. Data dianalisis menggunakan ujikorelasi.Rata - rata IMT subyek adalah 26.297 t 4.03 kglm2. Sebanyak 92.1% subyek mempunyai LPlebih besar dari normal. Rata-rata tekanan darah sistolik adalah 145.47 r 19.40 dan tekanandarah diastolik 87.33 t 8.75 mmHg. Rerata kadarAGTplasma adalah 79.4d0 xfi.Ag ng/mL.tidak terdapat hubungan yang bermakna antara IMT dan tekanan darah dan terdapat korelasiyang lemah dengan kadarAGT plasma (r=0.291, p=0.011). LP mempunyai korelasi lemahdengan tekanan darah sistolik (r=0.298, p=0.009), dengan TDD (r=0.298, p=6.999), dan jugadengan kadar AGT plasm a (r=0.347 , p=0.002).These result show a moderate correlation between BMI with plasma AGT levels in adultMinangkabau ethnic group. There is also a weak correlation between WC with BP and plasmaAGT levels.AbstrackPrevalence of obesity increased and related with increased of pasma angiotensinogen (AGT)levels and blood pressure. This study aimed to determine the correlation of antropometric withblood pressure (BP) and plasma AGT levels in adult Minangkabau ethnic.Seventy five adult, 35-54 years old, in Padang were enrolled randomely. All subjects wereinterviewed to determine the characteristics and physicai activity. Body fat percentagemeasurement, anthropometric (body mass inde/BMl and wrist circumference/ WC), bloodpressure and plasmaAGT levels examination were done. Data were analyzed using correlationtest.MKA, Volume 37, Nomor.Supl. 2, November 2014Desmawati:Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasaThe mean BMI is 26.297 t 4.03 kglm2. A total of 92.1% of subjects have WC larger than normalvalue. The mean of systolic blood pressure is 145.47 x 19.40 and diastolic blood pressure87.33 t 8.75 mmHg. The mean plasma levels of AGT 79.460 t 17.08 ng/ml. There was nosiEnificant relationship between BMI with blood pressure (BP), and it has weak correlation withplasmaAGT level {r=0.291, p=0.011) .WC has weak correlation with SBP (r=0.2g8, p=0.009),with DBP (r=0.298, p=0.009), and so with plasma AGT levels (r=0.347, p=0.002).These result show a moderate correlation between BMI with plasma AGT levels in adultMinangkabau ethnic group. There is also a weak correlation between WC with BP and plasmaAGT levels.
POLIMORFISTIE GEN TNF-q G-238A DAN IL-4 T-34C SERTA HUBUNGAN DENGAN KADAR IL-4 DAN TNF-q PASIEN PENYAKIT GRAVES Raveinal Raveinal; Siti Nurhajjah; Dwitya Elvira; Suci Maulida
Majalah Kedokteran Andalas Vol 37 (2014): Supplement 2 | Published in December 2014
Publisher : Faculty of Medicine, Universitas Andalas

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Abstract

Pendahuluan: Penyakit Graves adalah hipertiraidisme ditandai pembesaran tiroid difus dengan penyebab imunologi. Kondisi autoimun dengan adanya antibodi lmunoglobulin G (lgG) yang mengikat dan mengaktifkan reseptor tirotropin. Pemberian PTU dapat menurunkan hormon tiroid mencapai normal akan tetapi kadar lL-4 tidak pernah mencapai nilai normal. Penyakit Graves di Taiwan didapatkan polimorfisme gen promotor sitokin TNF-q G-238A, sedangkan gen IL-4 tidak berbeda dibanding kontrol sehat. Tujuan penelitian ini adalah mengetahui danmenganalis polimorfrsme TNF-o G-238A dan gen ll-i T-34C serta hubungannya dengan antibcCr tiroid, kadar lL-4 dan TNF-q pasien penyakit Graves. Metode: Penelitian ini merupakan penelitian deskriptif analitik, dengan jumlah sampel 35 orang pasien penyakit Graves di RSUP Dr. M. Djamil Padang. Darah vena diambil sebanyak 10 cc untuk isolasi DNA, kadar TNF-q dan lL-4. Pemeriksaan polimorfisme gen gen TNF-o G-238Adan IL-4 T-34C dengan teknik PCR-RFLP menggunakan enzym retriksi /'lzlsp/ dan Mnll serta dielektroporesis pada agarose 2%. Pemeriksaan TNF-q dan kadar IL-4 rnengunakan teknik ELISA. Analisis statistik untuk polimorfisme gen dengan uji Chi-Square dan untuk melihat hubungan antar variabel menggunakan uji ANOVA. Hasil: Didapatkan polimorfisme TNF-q G-238A dengan distribusi alel GG (91,4%), GA (8,6%) dan AA (0%). Frekuensi alel G adalah 95,99% dan alel A 4,3%.Rerata kadar TNF-q adalah 62,8+16,6 pg/ml. Polimorfisme gen lL-4 T-34C dengan distribusi alel TT t28,6 % ),TC ( 28,6 oA ), CC (42,9 %). Frekuensi alel T adalah 42,9 % dan alel C adalah 57,1 %. Rerata kadar lL-4 adalah sebesar 22,8 t9,4 pg/ml. Kesimpulan: penelitian ini dapat disimpulkan bahwa polimorfisme gen lL-4 T-34C lebih tinggi didapatkan pada penderita penyakit Graves dan terdapat kaiian antara polimorfisme gen inidengan peningkatan kadar IL-4. Kemungkinan adanya kaitan antara polimorfisme gen lL-4 T-34C dengan kejadian penyakit Graves.

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