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Published by Universitas Negeri Padang

ISSN : 2614669X EISSN : 2579308X DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.

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Articles 13 Documents

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Search results for , issue "Vol 4, No 1 (2020): Biology" : 13 Documents clear

Inventory of Ferns (Pteridophyta) at Cerme Cave Bantul District Zuchrotus Salamah; Hadi Sasongko; Aulida Zulaikha Hidayati
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Cerme Cave situated at Srunggo, Selopamioro village, Imogiri, Bantul District. Bantul has a supporting environment condition for ferns to grow and thrived. This research is conducted to produce an inventory of ferns grow within the Cerme Cave area. This research used explorative-method which include exploration, inventory, and fern identification activities. Descriptive analysis is used for data analysis. Result of this research showing that there are 17 species found within the Cerme Cave area, which are Drynaria quercifolia (Linnaeus) J. Smith, Pyrrosia longifolia (Burm. f.) C.V. Morton, Athyrium sp., Adiantum induratum Chirt, J. Bot. (Morot), Adiantum capillus-veneris Linn., Selaginella sp., Selaginella indica (Milde) R. M. Tryon, Adiantum philippense Linn., Pyrrosia petiolosa (Christ) Ching, Nephrolepis cordifolia (Linnaeus) C. Presl, Pteris vittata Linn., Pneumatopteris sp., Adiantum malesianum J. Ghatak, Hymenasplenium sp., Pneumatopteris sp., Pyrrosia nummularifolia (Sw.) Ching, Pteris sp.. Kata kunci: Cerme Cave, Pteridophyta

Effect of Hydrogen Peroxide (H2O2) on Different Levels of Water Availability on Vegetative Growth of Soybean Plants [Glycine max (L.) Merr.] Argomulyo Varieties Ika Shintya; Sri Widodo Agung Suedy; Endah Dwi Hastuti
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The soybean [Glycine max (L.) Merr.] is one of the important sources of vegetable protein and agricultural commodities in Indonesia, but its productivity is uncertain due to various factors, one of which is the availability of water. Low water availability in the growing environment can cause soybean plants to suffer from drought stress. Giving hydrogen peroxide to plants experiencing drought stress in optimum concentration can increase the plant's oxidative defense system. This study aims to determine the effect of treatment on the vegetative growth of Argomulyo variety soybean plants. The study used a factorial completely randomized design (CRD) design with 2x4 treatment and 5 replications. The parameters observed were plant height, number of leaves, leaf area, and fresh dry weight of the root canopy at the level of water availability 100% and 35% field capacity by spraying hydrogen peroxide concentrations of 0 mM, 0.5 mM, 1 mM, and 2 mM. The results were analyzed by ANOVA and DMRT test with a significance level of 0.05. The level of water availability influences plant height, number of leaves, leaf area, and fresh dry weight of the root canopy. The results show a 100% water availability level has a higher yield. Keywords: soybean, hydrogen peroxide, stress, growth

Changes of bird behavior in response to magnetic fields anomaly before the earthquake: a review Muhyiatul Fadilah; Riandi Riandi; Anna Permanasari; Enok Maryani
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Animal behavior is a response to external environmental conditions through detection by certain senses. Changes in physical factors in the environment before the earthquake (magnetic field anomaly) also influence the expression of specific behavioral changes in birds, namely the flight activity. However, scientific explanation of changes in orientation or direction of flight before the earthquake was not yet available. This article aims to describe the mechanism of bird's biological response to changes in the magnetic field before the earthquake. This study is a literature review. The results of theoretical synthesis explain that birds use magnetic receptors and light receptors that recognize changes in the magnetic field before the earthquake followed by an increase in the amount of intracellular calcium ions. Abnormal amounts of calcium stimulate the formation of more ATP which activates the function of actin and myosin, therefore muscle cells experience a bathmotrope effect expressed in the form of changes in flight direction.

Morphology Character and Andrographolide Quantifications on Sambiloto ( Andrographis paniculata (Burm.F.) Nees) Retno Prihatini; Auzar Syarif; Amri Bakhtiar
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202041107669-0-00

ABSTRACTThe Morphology character and andrographolide quantification on Sambiloto (A. Paniculata) that growth in Sawah Dangka Jorong Gaduik, Tilatang Kamang, Agam (S 00o16159.311 E100o23110.311) have been studied. The Morphology character of A. Paniculata on generative fase have been done in the field and Laboratorium of Biology Department, Andalas University. Quantification of andrographolide constituen were analysed by using High Performance Liquid Chromatography (HPLC) in Chemical of Nature Resources Laboratorium, Pharmacy Faculty, Andalas University. The result showed that Morphology character of Sambiloto ( A. Paniculata ) include parameters ie. range of height plant ( 27-45 cm) ; range of nodus numerous ( 10-14 ); range of leaf-7 size (length; width) ( 3.8-4.1 ; 0.8-1.1) cm; range of branch numerous ( 8-10), leaf dry weight rate (2.482 g ), stem dry weight rate ( 5.882 g ), and day old flowering rate ( 110 day ). The Result of quantification secondary metabolite with high economic value, namely andrographolide showed respectively with level 2.208% (vegetative) and 2.780 % (generative). Key Words : Andrographis paniculata, morpology chracter, andrographolide quantification ABSTRAKTelah dilakukan studi tentang Karakter morfologi dan kuantifikasi senyawa andrografolide pada tanaman Sambiloto (A. Paniculata) yang hidup di Sawah Dangka Jorong Gaduik, Tilatang Kamang, Agam (S 00o16159.311 E100o23110.311). Karakter morfologi A. Paniculata pada fase generative telah dilakukan di lapangan dan di Laboratorium di Jurusan Biologi Universitas Andalas. Kuantifikasi senyawa andrografolid dianalisa dengan menggunakan High Performance Liquid Chromatography (HPLC) di Laboratorium Kimia Bahan Alam, Fakultas Farmasi Universitas Andalas. Hasil menunjukkan bahwa karakter morfologi Sambiloto ( A. Paniculata ) meliputi parameter yaitu kisaran tinggi tanaman ( 27-45 cm) ; kisaran jumlah nodus ( 10-14 ); kisaran ukuran daunke-7 (panjang; lebar) ( 3,8-4,1 ; 0,8-1,1) cm; kisaran jumlah cabang ( 8-10), rata-rata berat kering daun (2,482 g ), rata-rata berat kering batang ( 5,882 g ), and rata-rata umur berbunga ( 110 day ). Hasil kuantifikasi metabolit sekunder dengan nilai ekonomi tinggi, yang dikenal sebagai senyawa andrografolid menunjukkan level 2,208% (vegetatif) dan 2,780 % (generatif). Kata kunci: Andrographis paniculata, karakter morfologi, kuantifikasi andrografolid.

Abundance Study of Koompassia excelsa in Maintaining Conservation of Ecosystems in Tangkahan Langkat Nature Reserve, North Sumatra Pandu Prabowo Warsodirejo; Asri Safitri Hasibuan; Arifin Ritonga; Lia Listiana
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Writing this paper aims to introduce the king's wooden tree (Koompassia excelsa) to the general public, as the background of this writing because North Sumatra especially in the Tangkahan Langkat forest has one tree with a height of almost 88 meters with very large roots and very hard wood, but not much people know it, this can happen because of lack of exposure and lack of publicity both directly and with the mass media. The method used in this study is the Exploration Method and directly record the sample during the trip. The area observed in the data collection process is 40 hectares divided into 2 regions, namely Region A (15 Hectares) and Region B (25 Hectares). The purpose of this study is to explain that the Koompasia excelsa species is an original species called Timber The king and saw its abundance in the Gunung Leuser Tangkahan Langkat Forest which is adjacent to the Natural Protection Forest of Bukit Barisan National Park in North Sumatra. Based on the results of research in the Gunung Leuser National Park Forest area, North Sumatra it can be concluded that the tualang tree can be found in lowland habitats with altitudes reaching 350 m above sea level and slope rates varying from 40-60. The dominance of species and associations is one of the factors that influence the adventure habitat, but that does not mean that other species do not influence the adventure habitat, such as altitude, soil type, and species of animals that have not been observed and analyzed further. In addition, it is necessary o carry out an inventory of adventurers to estimate the population in North Sumtatra, so that if there is a decline in population, conservation status can be improved.

Identification and Characterization of PrTK 2 Bacterial Isolate Producing Extracelular Protease Enzym From Tempeh Rubber Seeds Cico Jhon Karunia Simamora; Sukmawati Sukmawati
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Protease is an enzyme that can hydrolyze proteins into simpler compounds such as small peptides and amino acids. The enzymes produced by microorganisms can be isolated by separating cells by centrifugation. This study aims to determine the biological characteristics of the PrTK 02 bacterial isolate and the characteristics of the crude extract of the protease enzyme produced by the PrTK 02 bacterial isolate. The study was conducted by inoculating 1 ose PrTK 02 bacteria on the Nutrient Broth (NB) media. Pre-culture was carried out at 37 °C for 48 hours with an agitation speed of 120 rpm. Protease enzyme extraction was carried out by centrifugation of bacterial growth media at a speed of 3000 rpm for 15 minutes at 4 °C. Supernatant as a sample was tested for protease activity and protein content. The results of this study were obtained from the protease enzyme from bacterial isolate PrTK 02 marked by a clear zone in the medium of high school and the highest activity at the time of production 24 hours with the specific activity of the enzyme 1.2154 unit / mg protein. Biochemical and microscopic identification shows that the isolate of PrTk 02 bacteria is a class of Bacillus sp. PrTk 01 which is a Gram-positive bacteria that is found in tempeh.

Community Structure of the Mangrove Forest in the Tourism Area of Pariaman City, West Sumatra Meylia Alvareza; Irma Leilani
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The present study aims to determine mangrove community structure in the mangrove tourism area of Pariaman City. This study has been conducted in October to December 2019 using the purposive sampling method and belt transect in three stations. A total of 21 mangrove species, 20 genera and 19 families was identified in this area. The tree level vegetation of mangroves was dominated by Sonneratia caseolaris (INP = 140,23%). The sapling level vegetation of mangroves was dominated by Dolichandrone spathacea (INP = 174,39%). The seedling and understorey level vegetation of mangroves was dominated by Acanthus ilicifolius (INP = 58%). The diversity of mangrove species in this study area is relatively low to middle (H’ = 1,574 – 2,212).

Carbon Stock Of Seagrass In Karang Tirta’s Coastal Area, Padang Firda Az zahra; Chairul Chairul; Indra Junaidi Zakaria
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The seagrass beds are the spawning, nursery, and the feeding ground for some oceanic biota. It’s also can trapped the sediment and stabilized the substrate so the waters looks clear. Beside those abilities, seagrasses are also can store the carbon. The research was conducted in karang tirta’s coastal area, padang city to analyze the carbon stock of seagrass that stored within their biomass. The biomass was analyzed by harvesting the seagrass at transect 25x25cm, while the carbon stock was analyzed by using walkley & black method. Seagrass beds in karang tirta’s coastal has 14,1 ha (142.546,36 m2) distribution area and it’s stored 18,05 tons of carbon. based on these result the seagrass beds in karang tirta’s coastal area has stored 1,2 tons C/ha. the highest carbon stock has found in the below ground, especially in their rhizomes.

Analysis Of ZDS and LCYb Enzyme Coding Gene Related To Beta Carotene Bioshynthesis in Cassava (Manihot esculenta Crantz) using Reverse transcription PCR (RT-PCR) Jihan Rezi Okanti; Dwi Hilda Putri; Ahmad Fathoni
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

This study aims to determine variations in the expression of ZDS and LCYb enzymes encoding several types of yellow tubers. Cassava used, namely Carvita 25, Nangka and Mentega 2 (yellow bulb) and Menti (white bulb) were used as controls. The target enzyme coding genes (ZDS and LCYb) in cassava were identified in an online database, Phytozome using the BLAST method. Design and primary analysis for the target genes was carried out using online software, OligoAnalyzer and PCR. The expression of the target enzyme coding gene was analyzed using the Reverse Transcription PCR (RT-PCR) method. The BLAST results in the cassava genome in the Phytozome database showed that the enzymes ZDS and LCYb in cassava were encoded by two genes. The primary optimization results showed the primary annealing temperature for the LCYb enzyme coding gene was 55 ° C. The ZDS primers were not amplified after several replications, so that only the LCYb enzyme coding gene was selected for qualitative gene expression analysis using RT-PCR. A positive control in the analysis of gene expression is the housekeeping gene, PP2A. Electrophoresis results of PCR products (RT-PCR) showed negative results (no DNA bands were detected) in all samples from both yellow and white tuber samples and its housekeeping gene. This is possible from the low quality of RNA used in cDNA synthesis.

Enumertion of Enterobacteriaceae in Ice-Based School Children Food in Koto Tangah District, Padang West Sumatera Bob Refianto; Irdawati Irdawati
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Elementary students have high playing activities while in school. Hot weather and feeling tired after playing, will cause thirst and to get rid of thirst Elementary school students will buy ice-based snacks. They do not realize, the danger of unhygienic snacks will cause diarrheal diseases, because ice cubes are made by home industries still traditionally without considering hygiene requirements. In some countries, the food industry has moved towards testing Enterobacteriaceae which shows enteric bacterial contamination as a condition hygiene. This study aimed to determine the microbiological hygiene quality of the Enterobacteriaceae numbers in ice-based school children snacks in Koto Tangah Subdistrict, Padang City. This study was a descriptive study. The study was conducted from April to May 2019 at the Laboratory of Microbiology at the Center for Drug and Food Control in Padang. Total samples taken were 20 samples. The results showed that the sample that fulfilled the microbiological quality requirements for sadness was 60% (12 samples) and 40% (8 samples) that did not meet the requirements, the highest results were obtained at SDN 44 Sungai Lareh with a value of 9.82 x 105 colonies/mL.

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