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Bioscience
Published by Universitas Negeri Padang
ISSN : 2614669X     EISSN : 2579308X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.
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Articles 8 Documents
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Search results for , issue "Vol 8, No 2 (2024): Biology" : 8 Documents clear
PENGARUH PEMBERIAN PUPUK ORGANIK CAIR KULIT PISANG TANDUK (Musa paradisiaca) DAN DAUN Moringa oleifera TERHADAP PERTUMBUHAN TANAMAN KANGKUNG DARAT (Ipomoea reptans POIR.) DENGAN MENGGUNAKAN MEDIA KULTUR AIR Hayati, Annida Putri; Maharani, Dhea Tinde Fitriana; Rahmawati, Fitriana; Fauziah, Karyna; Helmi, Haidar; Zanah, Nurul Fatihal; Khairunnisa, Zahra Syafira; Rahman, Taufik; Suwandi, Tri
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371121503-0-00

Abstract

Kangkung darat (Ipomoea reptans Poir) adalah salah satu jenis kangkung yang mudah dibudidayakan meskipun begitu, di kota besar sulit menanam kangkung karena keterbatasan lahan. Digunakanlah metode kultur air dalam budidayanya. Penelitian ini bertujuan untuk mengetahui pengaruh pupuk organik cair (POC) berbahan dasar kulit pisang (Musa paradisiaca) dan daun kelor (Moringa oleifera) terhadap pertumbuhan kangkung darat dengan menggunakan metode kultur air.  Penelitian dilakukan selama 14 hari, di laboratorium Fisiologi FPMIPA Universitas Pendidikan Indonesia. Penelitian ini menggunakan metode Rancang Acak Lengkap (RAL) dengan 3 kali pengulangan dari setiap perlakuan. Parameter penelitian ini yaitu jumlah daun, tinggi cabang, panjang akar, jumlah cabang, berat basah batang dan berat basah akar. Data dianalisis dengan menggunakan uji Anova pada taraf 95%. Dari hasil penelitian diketahui bahwa pupuk POC tidak memiliki pengaruh nyata terhadap jumlah daun, tinggi cabang, panjang akar, jumlah cabang, berat basah batang dan berat basah akar. Tetapi pengaruh dapat dilihat apabila dibandingkan dengan kontrol.Kata kunci: Kangkung darat, Kultur air, Musa paradisiaca,  Moringa oleifera
Effecacy of Black Sugarcane Leaves (Saccharum officinarum L.) as Anti-Dysmenorrhea In Silico Based On Molecular Docking Fadilaturahmah, Fadilaturahmah; Dinata, Marta; Sari, Martala; Joni, Rahma; Kurniati, Rahmi; Ade, Filza Yulina
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i2.131039

Abstract

Dysmenorrhea is menstrual pain in women that interferes with daily activities. Dysmenorrhea causes decreased productivity, performance, sleep disturbances, and quality of life. The use of antidysmenorrhoea drugs such as non-steroidal anti-inflammatory drugs, oral contraceptive pills have many side effects in the form of nerve disorders, blood vessels, and even heart failure. Therefore, the exploration of natural medicines is essential. One of the traditional medicinal ingredients that is thought to have efficacy in relieving menstrual pain is black sugarcane leaves (Saccharum officinarum L.). Testing the efficacy of this medicinal ingredient can be done in silico using computational biology technology. This in silico study is effective in drug discovery efforts because it is cost and time-efficient. This study aims to analyze the ability of black sugarcane leaf phytochemical compounds to inhibit the dysmenorrhea pathway's activation based on molecular docking. This observational study is based on bioinformatics using the molecular docking method. Research data were obtained from analysis using software computing programs such as the National Center for Biotechnology Information, prediction of activity spectra of substances, Lipinski rule of five tests, pyrx, and discovery studio. The research data were analyzed based on the binding affinity value and visualization of the molecular docking results. Black sugarcane leaves contain 27 phytochemical compounds based on GCMS analysis with various bioactivities related to anti-dysmenorrhea (menstrual pain). 16 compounds have activity probability values (Pa) > 0.7, which indicates that their bioactivity is analogous to that of commercial drugs (natural effective drug candidates). The hexadecanoic acid compound is the best anti-dysmenorrhea drug candidate compared to other phytochemical compounds in black sugarcane leaves because it has the lowest binding affinity value of -6 kcal/mol.Dismenorea merupakan nyeri menstruasi pada wanita yang mengganggu aktivitas sehari-hari. Dismenorea menyebabkan penurunan produktivitas, penurunan prestasi, gangguan tidur, dan penurunan kualitas hidup. Penggunaan obat antidismenorea seperti antiinflamasi non steroid, pil kontrasepsi oral memiliki banyak efek samping berupa gangguan saraf, pembuluh darah, bahkan gagal jantung. Oleh karena itu, eksplorasi obat berbahan alami sangat diperlukan. Salah satu bahan obat tradisional yang diduga memiliki khasiat dalam meredakan nyeri menstruasi yaitu daun tebu ireng (Saccharum officinarum L.). Pengujian khasiat bahan obat ini dapat dilakukan secara in silico menggunakan teknologi biologi komputasi. Kajian secara in silico ini efektif digunakan dalam upaya penemuan obat karena efisien biaya dan waktu. Penelitian ini bertujuan untuk menganalisis kemampuan senyawa fitokimia daun tebu ireng dalam menghambat aktivasi jalur dismenorea berdasarkan molecular docking. Penelitian ini merupakan penelitian observasi berbasis bioinformatika dengan menggunakan metode molecular docking. Data penelitian diperoleh dari analisis menggunakan program komputasi software seperti National Center for Biotechnology Information, prediction of activity spectra of substances, lipinski rule of five test, pyrx, dan discovery studio. Data hasil penelitian dianalisis berdasarkan nilai binding affinity dan visualisasi hasil molecular docking. Daun tebu ireng mengandung 27 senyawa fitokimia berdasarkan analisis GCMS yang memiliki berbagai bioaktivitas terkait dengan anti-dismenorea (nyeri menstruasi). 16 senyawa memiliki nilai probabilitas activity (Pa) > 0,7 yang mengindikasikan bahwa bioaktivitasnya analog dengan obat komersil (kandidat obat efektif alami). Senyawa hexadecanoic acid merupakan kandidat obat anti-dismenorea terbaik dibandingkan senyawa fitokimia lainnya pada daun tebu ireng karena memiliki nilai binding affinity terendah yaitu -6 kkal/mol. 
PRODUCTION POTENTIAL OF VEGETATIVE SEED THE MEXICAN SWORD PLANT (Aquarius palifolius (Nees & Mart.) Christenh. & Byng. Family; Alismataceae Rizki, Rizki; Rasdanelwati, Rasdanelwati; Alfina, Rina; Darlis, Olivia
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i2.130418

Abstract

Vegetative seed production is an alternative for propagating the Mexican Sword Plant, apart from generative propagation using seeds. The vegetative seeds of this plant are unique because they are produced from the development of compound flower stalks (inflorescence). The use of vegetative shoots that appear on the flower stalks of the Mexican Sword Plant is an attractive and efficient alternative in producing seeds for this plant. Apart from that, research on vegetative production is urgent in increasing the production and availability of Mexican Sword Plant seeds. This research was carried out for three months. The study aimed to determine the potential for vegetative seed production of the Mexican Sword Plant-sourced from the shoots of compound flower stalks or inflorescences, which includes the number of main inflorescence axes formed, the number of inflorescence stalks that produce branches, the number of nodes formed on the primary axis inflorescence, the number of buds formed both on the central axis and on the branches of the inflorescence. This research is a descriptive study with observations carried out in the experimental greenhouse of the Politeknik Pertanian Negeri Payakumbuh, Lima Puluh Kota Regency. The parental data was obtained from the results of previous vegetative seed propagation, which was approximately two years old and had been repotted with 30 plants. The media used is a mixture of rice field mud and cow manure using a pot with a diameter of 30 cm. Based on the research that has been carried out, it is known that the central axis of inflorescences formed are 81 inflorescences, 30% each with two inflorescences and 70% with three inflorescences. Five hundred seventy-two nodes (nodes) formed on the central axis, and 488 nodes on the inflorescence branches. The total number of shoots formed was 741, and the average shoots per planting were 25.
Observation Test of Durian (Durio zibethinus Murr.) from Several Regions in West Sumatera Indriyani, Ni Luh Putu; Ihsan, Farihul; Hadiati, Sri; Budiyanti, Tri; Riska, Riska; Jumjunidang, Jumjunidang; Mansyah, Ellina; Muas, Irwan
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i2.129070

Abstract

The open pollinating of durian produces diversity that allows for indigenous selection. The aim of the research is to raise the potential of the genetic resources of West Sumatran durian to become a new superior variety. Observation tests were carried out in Sijunjung, Tanah Datar, Padang Pariaman, Sawahlunto, and Pesisir Selatan The variables observed were qualitative and quantitative characters, using the descriptor for durian and guidelines for preparing horticultural variety descriptions. Observations of qualitative and quantitative characters were carried out on plants, stems, leaves, flowers and fruit. From the results of the observation test, 4 new superior varieties have been registered, namely Kalumpang Sijunjung with Minister of Agriculture Decree No: 039/Kpts/SR.120/ D.2.7/6/2014, Tambago Sungai Tarab with Minister of Agriculture Decree No:015/Kpts/SR.120/D.2.7/2/2016, Kunik Tandikek with Minister of Agriculture Decree No: 258/Kpts/SR.130/D/III/2021, Sawah Kubang with Minister of Agriculture Decree No:225/Kpts/PV.240/D /VI/2023 and one prospective of durian variety of Pesisir Selatan which which is currently being processed in registering local varieties. In order for new varieties to develop, there needs to be a development program from local governments to stakeholders.  Apart from that, activities to increase the potential of durian genetic resources in West Sumatera need to continue.
King of Bitter (Andrographis paniculata Nees) Somatic Embryogeni Callus Culture on MS Medium With Added by 2,4-D and BAP Prihatini, Retno; K.Huda, Nadyatul; Idris, Muhamad
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i2.130950

Abstract

In this research we have studied about somatic embryogenesis derived on Sambiloto (Andrographis paniculata) cv. VGT callus culture by addition BAP (benzylamino purine) and 2,4-D (2,4 diclorophenoxyacetic acid)   on MS (Murashige and Skoog) Medium. King of Bitter seeds were germinated on MS medium for 3 weeks, then  the second leaf  that full opened used as explants. The explant planting  on medium with several concentration growth regulator (BAP combinated with 2,4-D) to callus induce.  MS medium  with added by 0.5 ppm BAP+ 0.5 ppm 2,4-D  ( A treatment), 0.5. ppm BAP + 1 ppm 2,4-D (B treatment ), 0.5 ppm BAP + 1.5 ppm 2,4-D (C treatment ), 0.5 ppm BAP + 2 ppm 2,4-D (D treatment), each of treatment with 5 replications. The observation used descriptive method for callus texture, somatic embryo derived with it development fase  after subcultured on MS medium with the same concrentration BAP  and 2,4-D . The result showed that callus  have formed on  all of treatment (A, B,C and D), whereas callus with somatic embryogenesis only on C treatment, with the range of callus fresh weight (FW) were 0.6-1.0 g.  After 3 weeks at second subcultured on MS medium with added 0.5 ppm BAP+ 1,5 ppm 2,4-D ,  somatic embryo  development showed  globular fase  and heart shape fase .
Litter Decomposition on the Old Parak Ecosystem in Maninjau West Sumatra Indonesia Kardiman, Reki; Simangunsong, David
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i2.125864

Abstract

This study aimed to analyze the rate of litter decomposition on the old parak ecosystem in Maninjau, Tanjung Raya District, Agam Regency, West Sumatra Indonesia. Data were collected at four different vaillages, each was located at one compas point surrounding the Maninjau Lake. The sites were purposively selected according to the zonation of vegetation, where the parak ecosystem appeared between paddy field and forest. Three of 20x20 m plots were established at each location, in which one of 1x1 meter subplot was established in the centre, given 12 subplots in total. All litters within the subplots were collected and measued the wet weight, about 800 grams of the litters were collected, 200 grams of it were dried, while other 600 grams were placed into six litter bags (100 grams each) and placed again at each subplot. Two litter bags were collected each month, dried and analyzed the rate and percentage of decomposition. The results showed that the average litter decomposition rate at parak ecosystem in Maninjau was 0.6 gr/day, and 50% litters were decompossed in 90 days. The rate of decomposition of litter varied between loactions, where the lowest decomposition rate was found at the western part of the Maninjau Lake, especially for leaves litters. This study revealed that a park ecosystem in Maninjau requires six month decompotition to completely change litters into soil, and with about 6.4 tons of litters per hectar, resulting 5 tons of biomass, this provides 5 tons of organic matters for each hectare of parak ecosystem.
Literature Review: Benefits and applications of alginate liase enzyme) Sirwati, Fadila; Putri, Dwi Hilda; Rachmayati, Rike
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i2.131077

Abstract

Alginate is a polymer found in large amounts in the cell walls of brown algae. This polymer consists of α-L guluronic acid (G) and mannuronic acid (M). Alginate can be degraded by an enzyme, known as alginate liase by removing the glycosidic bond and producing an unsaturated oligosaccharide with a double bond at the non-reducing end. To provide an overview of the utilization of alginate lyase, information is needed on the biological activity of alginate lyase as an antibiofilm agent, the production of alginate oligosaccharides, and its antioxidant properties. This study aims to provide scientific insight into the important role of the benefits and applications of alginate lyase. The research design used was a literature review. Articles were collected based on sources from PubMed, Science Direct, and Google Scholar, which included more than thirty national and international journals. The articles collected showed that alginate lyase exhibits many biological activities, including antibiofilm, antioxidant, and oligosaccharide production. These properties have the potential to be used in a wide range of applications, which include the food, pharmaceutical, and biotechnology industries.
Conflict between Trees and Infrastructures in the Main Campus ofState University ofPadang Rahman, Natasya Aulia; Kardiman, Reki; Roza, Sri Yenica
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i2.125870

Abstract

Infrastructure is major supporting factor development a state and trees are planted throughoutthe infrastructure for the green aspect, which then promotes a conflict. Conflicts defined as tree growth atadjacent infrastructure,growth of roots causing a damages as in the main campus of State University ofPadang. This study to investigate the conflict between trees and infrastructure in the main campus of StateUniversityofPadang.Treespecies,diameter,size,canopy,distancetreetoinfrastructure,numberinfrastructureandtypeinfrastructureweresampledfrom195treesconflictandnotconflict.Alltreesbelongingto 12 species diameterfrom 26,75 cmto 114,01 cm(58,8392 cm onaverage), and canopyfrom300 m² to 825 m² (555,7692 m² on average). Trees planted to concrete by 71%, 33% conflicted, paving blockby 21%, 12% conflicted, and 8% to asphalt with 55% conflicted. Conflicted trees varied species but mostlyplanted <1 m from infrastructures, tended to larger diameter and narrow canopy cover. This study showedthere is 50% risk infrastucture damage planted close and tree species with lower potential conflict prioritizedforshade treesacrossinfrastructure. 

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