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Gilang Nugraha
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ijmlst@unusa.ac.id
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INDONESIA
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Published by Universitas Nahdlatul Ulama Surabaya
ISSN : 26846748     EISSN : 26569825     DOI : https://doi.org/10.33086/ijmlst
Core Subject : Health, Social,
Health Professions Social Sciences
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
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Articles 2 Documents
 1 
Search results for , issue "vol 8 no 2 (2026): in progress" : 2 Documents clear
Efficiency and challenges of exosome and microRNA extraction from clinical biofluids in Indonesia: A commercial kit-based approach for qPCR applications Aprilia Indra Kartika; Muchamad Dafip; Nastiti Wijayanti; Didik Setyo Heriyanto; Sofia Mubarika Haryana; Kartika Widayati Taroeno-Hariadi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 2 (2026): In Progress
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i2.7350

Abstract

Exosome miRNA has significant potential as biomarker for minimal or non-invasive diagnosis and monitoring of disease progression. However, extraction of miRNA exosomes from biofluids is challenging, and there is a lack of comparative evaluation of commercially available extraction kits for qPCR applications. Therefore, this study aims to obtain information about miRNA exosome extraction using commercial kits and analyze its expression with the qRT-PCR method. The exosome extraction was carried out using 3 types of commercial kits, while the RNA extraction was performed with a spin column. The purity and concentration of exosomes were measured using NTA, while total RNA was measured with nanodrops. Subsequently, miRNA expression was measured using qRT-PCR. The results showed that the concentration of exosomes obtained using kit 1 for plasma isolation was 3.5 x 1011 with particle sizes ranging from 30-240 nm. Kit 2 yielded a value of 2.9 x 1010 with particle sizes ranging from 30-150 nm, while kit 3 had no measurable value. The average concentration of RNA extracted using the RNA Basic kit from HansaBioMed was 42.53 ng/μL, while the exoRNeasy Midi kit from Qiagen yielded 45.28 ng/μL. Template RNA of 100 ng was converted into cDNA, while the cDNA was diluted at a ratio of 1:20 using RNase free water during the quantification process of miRNA exosome expression with the qRT-PCR method. Based on the results, exosome miRNA quantification process requires the use of the correct method at each stage to obtain the right expression for clinical studies.
Saffron and folic acid mitigate hepatic structural damage in IUGR rats via modulation of oxidative stress markers: A histological and molecular study Nur Aini Hasan; Viskasari Pintoko Kalanjati; Iswandi Erwin; Lucky Prasetiowati; Joni Susanto; Muhammad Miftahussurur
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 2 (2026): In Progress
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i2.7919

Abstract

One of the major causes of Intrauterine Growth Restriction (IUGR) in Indonesia is maternal malnutrition during pregnancy. Folic Acid has long been the primary supplement administered to women from preconception through pregnancy. Additionally, several studies have demonstrated that Saffron possesses anti-inflammatory and hepatoprotective properties. However, the effects of Saffron on liver tissue in IUGR rat models remain underexplored. This study aimed to investigate the effects of Saffron administration compared to Folic Acid on birth weight, hepatic tumor necrosis factor-alpha (TNF-α) expression, and liver histopathology in IUGR rats. Forty female and eight male rats were mated. Upon confirmation of pregnancy, the dams were randomly assigned to four groups: normal control (K1), receiving a standard diet throughout pregnancy (19-21 days) and lactation (21 days); IUGR control (K2), subjected to 50% dietary restriction (DR); IUGR + Saffron group (K3), receiving DR plus Saffron extract at 15.68 mg/kg body weight; and IUGR + Folic Acid group (K4), receiving DR plus Folic Acid at 1.5 mg/kg body weight. After 21 days of lactation, the offspring were euthanized, and liver tissues were collected for histological and immunohistochemical analyses. Pups' birth weights, hepatic TNF-α expression (immunohistochemistry), and histological damage (NAS score) were assessed. Folic acid showed superior effects in increasing birth weight, reducing TNF-α, and improving liver histology compared to saffron. These findings suggest folic acid as a potent hepatoprotective agent in IUGR conditions.

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