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INDONESIA
Jurnal Pengendalian Hayati
Published by Universitas Jember
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal Pengendalian Hayati merupakan jurnal pengetahuan yang menyajikan artikel mengenai hasil penelitian serta perkembangan mutakhir pengendalian organisme pengganggu tumbuhan dengan memanfaatkan agensia pengendali hayati baik terhadap hama, penyebab penyakit dan gulma.
Arjuna Subject : -
Articles 10 Documents
 1 
Search results for , issue "Vol 1 No 1 (2008)" : 10 Documents clear
Pemanfaatan Mikroorganisme Antagonis untuk Mengendalikan Penyakit Hawar Bakteri (Xanthomonas campestris pv. malvacearum) pada Kapas Titiek YULIANTI; Nurul HIDAYAH
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

Bacterial blight of cotton caused by Xanthomonas campestris pv. malvacearum is oneof the important cotton diseases. The pathogen infects all of part of the plant from seedling to fullydevelop plant. In South Sulawesi, bacterial blight caused severe damage to the plant resulting yieldlost up to 50 %. Biological control is now a popular control method for plant diseases.This studyaimed to explore bacterial antagonists of bacterial blight. Exploration was conducted in Asembagus,Singosari and Karangploso. Bacteria were isolated from soil and cotton plant parts (root, sleaf, andstem). Six of 140 isolates were able to inhibit the growth of X. campestris pv. malvacearum in vitro.However, only 2 isolates (Asb-D-3 and Asb-D-5) have capability of reducing the disease severity on48,48% and 45,64% respectively.
Pengendalian Penyakit Layu pada Pisang dengan Bakteri Antagonis Pseudomonas fluorescens dan Bacillus subtilis Paniman Ashna MIHARDJO; Abdul MAJID
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

Fusarium wilt on bananas caused by the fungus Fusarium oxysporum f.sp cubense wereimportant and were distributed most of the centre of bananas farm in Indonesia. Both Culturetechnicaly and chemically were unable to control this pathogen because they ability to formClamydospore and to infect alternative hosts. The use of antagonistic bacteria such as Pseudomonasfluorescens and Bacillus subtilis were able to control plant pathogens both in rhizosphere or inphyllosphere because their ability to produce secondary metabolites such as siderophore, antibiotics,volatile substances, Cyanide acid, extracellular enzymes and phytohormones. This research wasconducted in Laboratory of Plant Pathology and greenhouse of Pest and Plant Diseases Department.The steps of research included exploration of antagonistic bacteria using Schaad methods,identification using Schaad, Palleron, Lelliot and Stead Methods. Last steps were done by in vitro andin vivo tests.The result showed that combination of two bacterias were able to inhibit the pathogen invitro (70,2% - 88,1%), while in vivo test with three to four applications in frequency were able todecrease disease intensity up to 81, 6%.
Biologi Parasitoid Koinobiont Opius sp. (Hymenoptera: Braconidae) Pada larva Lalat Pengorok Daun Liriomyza huidobrensis (Diptera: Agromyzidae) Hari PURNOMO; Sigit PRASTOWO; Moh. Wildan DJADMIKO; Shohab MABDUH
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

Opius sp. is koinobiont endoparasitoid of L. huidobrensis larvae. Opius sp. has shortlife cycle, high fecundity and able to parasite at different larvae stages. The research objective was todetermine effect of larvae stages of LMF on parasitsm, fitness, development time and longevity ofOpius sp. The research was conducted at Laboratory of Biological Control and Glass house of Pestand Plant Diseases Departement, Agriculture Faculty, Jember University from from April until July2007. The insect were collected from Ngadisari, subdistrict of Sukapura, Probolinggo. The experimentwas done on two methods, choice and non choice test. Percentage of parasitization, the sting time,development time, longevity, sex ratio, body lenght, hind and hind tibia of adult female and male ofparasitoid Opius sp were examined. The result showed that the sting time of Opius sp. was influencedby larvae. The lates stages of LMF has the higher sting time compare early stages, 44,28 s on firstinstar, 54,9 s on second instar, and 80,68 third instar respectly. Parasitization of parasitoid Opius sp.was not influenced by larvae stage of L. huidobrensis. The percentage of parasitization on choice testwas 28,49% on first instar, 17,43% on second instar and 14,95% third instar. On the other hand, onnon chice test was 48,21% on first instar, 54,52% on second instar and 54,00% on third instar. Sexratio, adult fitness, body length, hind and hind tibia length, development time, longevity wereinfluenced by larvae stages.
Pemanfatan Beberapa Sumber Bahan Organik Untuk Meningkatkan Kemampuan Trichoderma harzianum DC105 dalam Mengendalikan Penyakit Rebah Kecambah pada Kapas Titiek YULIANTI; SUPRIYONO .
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

Recently, antagonistics microbial have been using to control plant diseases because of theirsafety to environments. Mikroba antagonis saat ini banyak digunakan untuk mengendalikan penyakittanaman karena dianggap ramah terhadap lingkungan. Namun, penggunaan mikroba antagonisdalam skala lapang seringkali kurang berhasil karena tidak mampu beradaptasi dengan lingkunganyang baru. Penelitian ini bertujuan untuk meningkatkan kemampuan adaptasi isolat Trichodermaharzianum DC105 koleksi Balittas yang diketahui memiliki kemampuan mengendalikan Sclerotiumrolfsii dan Rhizoctonia solani penyebab penyakit rebah kecambah pada kapas dengan menambahkanbahan organik. Bahan organik yang digunakan berasal dari pupuk kandang ayam, blothong, ampastebu baru dan ampas tebu lama. Bahan organik tersebut dicampur dengan konidia T. harzianum untukdibuat pelet kemudian disimpan dalam suhu kamar atau suhu 5 oC sebelum diaplikasikan. Isolatdigunakan adalah isolat mutan T. harzianum yang resisten terhadap benomyl. Hasil pengujianmenunjukkan bahwa suhu penyimpanan tidak mempengaruhi viabilitas T. harzianum DC105 meskidisimpan sampai 6 bulan. Kemampuan menekan S. rolfsii dan R. solani terbaik diperoleh ketika isolatT. harzianum DC105 dicampur dengan pupuk kandang ayam, yaitu masing-masing 67% dan 71%.Meskipun viabilitas tidak berubah ketika disimpan sampai 6 bulan, namun kemampuan menekan S.rolfsii dan R. solani cenderung turun ketika pelet disimpan 6 bulan.
Viabilitas Nematoda Entomopatogen Steinernema spp. dalam Media Kombinasi Senyawa Humik - WAGIYANA; Didik SULISTYANTO; Sugeng WINARSO
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

Its well known, That Entomopathogenic Nematods (EPNs) used as biological agentswhich effective to control pest of Spodoptera litura (Noctuidae: Lepidoptera).The aims of thisresearch were to find out the multifungsion product as well as a biofeltilizer and biopesticide whichsuitable for agriculture sustainability, secondly the materials were to mixed the humic compound andbiological agent Entomopathogenic Nematodes (EPNs). Combination of biopesticide with EPNs asactive ingredient and humic compound as biofertilizer to find out a good product combination whichindicated the highest viability and activity of EPNs Steinernema spp on humic compound, in orderapplication of these meterial more effective and efficient to prepare the soil fertility and pest problem.This research was done with inoculation of EPNs on the medium/culture e.c: phosphate solublebacteria Pseudomonas putida 27.4B, zeolit and the humic compound (liquid culture). Pathogenecitytest of EPNs were done after incubation on the humic compound to the larvae of S. litura.The result of this research showed that long time of viability EPNs only for fourth weeks on the humicculture at (4000 ppm). Actually the EPNs from this incubation could 100 % mortality of the S. lituralarvae after 72 hours inoculation. The infection rate of EPNs on the larvae S. litura was 30,3 tailsduring 24 hours incubation. The viability of EPNs Steinernema spp on the medium with 1000 ppmhumic compound was 20 % after 120 hours and after 4 weeks later all EPNs could not survive on thismedium. It seems, the medium containing zeolith, P. putida 27.4B and humic compound at 1000 ppmwas not suitable medium neither for nematods growth or larvae S. litura survival.
Pemaduan Pseudomonas putida 27.4B dan Trichorderma sp. dalam Media Cair Senyawa Humik yang Diberi Zeolit untuk Mendapatkan Produk Multifungsi Ramah Lingkungan Sugeng WINARSO; Eko HANDAYANTO; SYEKHFANI .; Didik SULISTYANTO
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

Phosphate solubilizing bacteria (Pseudomonas putida 27.4B) is effective in dissolving undissolve P;humik compound can decrease Al exchange in acid soil and can be used as microorganism liquidmedia; Zeolite can improve CEC and bases concentration; and Trichorderma sp. can control diseaseand increase crop yield. Therefore, aim of this research is to make product having multifunction bothenvironment friendly soil amandement (fertilizer) and biopesticide, combination Pseudomonas putida27.4B, humic compound, zeolite, and Trichorderma sp.Potetoes Dextro Agar (PDA) media used to see resistance and growth, continued humic compoundliquid media added zeolite. Research design is completele randomized design with three factor are: 1)Trichorderma sp. concentration are: 0 (control); 104 CFU.ml-1; 105 CFU.ml-1; dan 106 CFU.ml-1; 2)good combination obtained last research are: a). 1000 ppm humic compound; 0,1% zeolit; P. putida27.4B 1012 CFU.ml-1 and b). 5000 ppm humic compound; 0,1% zeolit; P. putida 27.4B 1012 CFU.ml-1; and 3) incubation time are: 2 and 4 weeks.Result of research indicate that combination Pseudomonas putida 27.4B and Trichorderma sp. inPDA media can grow together, with visually speed of Trichorderma sp. growth faster thanPseudomonas putida 27.4B. Pseudomonas putida 27.4B proven can live on, even very good, in humiccompound liquid media added zeolite and Trichorderma sp. Its best growth is at humic compoundconcentration 1000 ppm. Besides that is tendency of combination Pseudomonas putida 27.4B andTrichorderma sp. exactly can increase growth of Pseudomonas putida 27.4B both at humic compoundconcentration 1000 ppm and without addition of humic compound. While at humic compoundconcentration 5000 ppm, Trichorderma sp. inokulasi exactly decrease Pseudomonas putida 27.4B.
Kajian Pemberian Stretomyces Spp Terhadap Perkembangan Gejala Penyakit Layu Fusarium Pada Tanaman Melon (Cucumis Melo L.) Penta SURYAMINARSIH; Tri MUJOKO; Dewi ANGGRAENI
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

In the century, chemistry method often was done to control fusarium wilt deseases inMelo (Rukmana dan Saputra 1997). It has negative effect to the environment (Novizar, 2002). Once ofthe control method that is often been researche is antagonis microorganism using and streeptomyces isbiological agens which is in actinomycetes group. Fusarium fungi can survive many years asClamidospora in the soil (Semangun 1994 and Kranz 1977). Appearing wilt symptom caused byFusarium infection in parenchym of the bottom stemp. Using of Streeptomyces as biological agenscan control spreading of Fusarium wilt symptom.The aim of the research was to know optimum dosage of streeptomyces givedto fusarium wiltsymptom in melo plant (Cucumis melo L.). The research had been done for six months, December2006 until May 2007 at experimental land of UPN “Veteran” East Java. Completely Randomizedesign was used. The Treatment was streeptomyces dosage consist of 2 gr/3 Kg soil. 4 gr/3 Kg, 6gr/3kg, 8 gr/3kg, 10 gr/3 Kg, and every treatment was done five times. Streptomyces isolation wastaken from 5 – 15 Cm deep soil of Wajak – Tulungagung.Observation was done once time a week toWilt leaf Symtom and discoloration length of steem.Based of the observation, The Conclution is 6 grStreeptomyces/3 Kg soil more effective than the other dosage of streeptomyces gived.
Pengaruh Sumber Karbon Terhadap Daya Antagonistik Bakteri Pseudomonas Pendar Fluor Terhadap Erwinia Carotovora Hardian Susilo ADDY
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

This research was conducted to study antimicrobial stimulation of fluorescentpseudomonad to inhibit Erwinia carotovora. We used several carbon sources to stimulateantimicrobial substances from fluorescent pseudomonad that available to inhibit E.carotovora in vitro.The results shown that mannitol 10% was the best stimulant agent to increase antagonistics offluorescent psudomonad againts E. carotovora. Also, mannitol increased antimicrobial substancestwohold compared with control without stimulant agent. Detection of antimicrobial substance usingTLC showed that only one antimicrobial was detected with retention factor (Rf) of 0,68. However,identtification and characterisation of that substance is needed.
Kemampuan Antagonistik Beberapa Isolat Pseudomonad Fluoresen Terhadap Bakteri Ralstonia Solanacearum Penyebab Penyakit Layu Pada Tanaman Tomat Yenny WURYANDARI; Arika PURNAWATI; Triwidodo ARWIYANTO; Bambang HADISUTRISNO
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

Bacterial Wilt disease (Ralstonia solanacearum) is a main problem in tomato plant.Controls of it have been optimum success yet. Control of it using certainly pseudomonad fluorescentstrain can pressure plant disease developments which cause soil pathogen.The aim of the research, getpseudomonad fluorescent from tomato rizosfer which can inhibit of wilt bacteria disease Ralstoniasolanacearum. Research methods are isolation and identification of pathogen bacteria andantagonistic bacteria. Biological control agents candidate selection was done with antagonistic invitro and inhibit mechanism test. The Research show; there is wilt symptom in tomato areal at WajakMalang. From isolation get Ralstonia solanacearum bacteria with identity white coloni, fluidal,irregular shape in YPGA media and high virulensi. From tomato rizosfer soil isolation in the sameareal get 130 isolate of pseudomonad fluorescent in King’s B media. Antagonistec test in vitro to itisn’t of all bacteria can inhibit Ralstonia solanacearum growing. From them which was tested, getvariation of inhibit zona from 4 mm until more than 30 mm. More of inhibit mechanism isbacteriostatic and only many is bacterisida.
Pemanfaatan Ekstrak Kompos dan Jamur Antagonis Trichoderma sp. untuk mengendalikan Powdery mildew pada Apel (Malus sylvestris Mill.) Mutia Erti DWIASTUTI; Ahmad SUYOSO
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

Powdery mildew disease caused by Podosphaera leucotricha is one of the key diseaseon apple trees. This disease wide spread on East Java and crop loss caused this disease for about 50%per year. The chemical control methods are not always economical or effective. Another promisingalternative is application of biological control. Trichoderma spp. are potencially as biological controlagent because its has strong antagonis characteristic. The purpose of this experiment was to determinethe optimal straw composted extract and effectivity of Trichoderma spp. isolates antagonis fungus tocontrol Powdery mildew on apple trees. The first phase was arranged in completely RandomizedDesign in laboratory by in vitro tested to determine the best isolates of fungus for futher tested. Thesecond phase was field experiment was used Randomized Block Design, two factors. The first factorwere composted extract with different cattle faeces stater and the second factor were Trichodermaspp. isolates and one control. The result of experiment indicated that laboratory and field experimentcould be control Powdery mildew. T3 is the best isolate for decrease this disease. Composted extractshowed that decrease effect to disease control in the laboratory, but didnot on the field. Strawcomposted extract with cattle faeces stater and combination of straw composted extract with cattlefaeces stater could be pursued Powdery mildew (13,88% disease intensity).

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