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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 26 Documents
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Search results for , issue "Vol 26, No 1 (2019)" : 26 Documents clear
Comparison of Lipid Fractions of Icteric Sample by Using Three Devices Ummul Khair; Asvin Nurulita; Darwati Muhadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1412

Abstract

COMPARISON OF LIPID FRACTIONS OF ICTERIC SAMPLE BY USING THREE DEVICES Ummul Khair1, Asvin Nurulita2,3, Darwati Muhadi2,31                      Specialist Doctoral Education Program of Clinical Pathology Science, Faculty of Medicine, Hasanuddin University/RSUP Dr. Wahidin Sudirohusodo, Makassar 2                      Department of Clinical Pathology Science, Faculty of Medicine, Hasanuddin University/RSUP Dr. Wahidin Sudirohusodo, Makassar3RSUP Dr. Wahidin Sudirohusodo, Makassar                                                                                ABSTRACTIntroductionLipid fraction assessment in laboratory includes cholesterol, HDL, LDL, Triglycerides. The icteric sample is characterized by elevated levels of bilirubin and darkness yellow sample color. This research was to determine the comparison of lipid fraction of icteric sample by using three devices.MethodCross-sectional study at Clinical Pathology Laboratory of Dr. Wahidin Sudirohusodo of Makassar. Comparison of lipid fraction of icteric samples against 3 (three) devices (Pentra, Biomajesty, Conelab) in period April - June 2018. Statistical analysis using SPSS program.Result and Discussions Total sample of 50 indicated that cholesterol level by Biomajesty was lower than Pentra400 (p < 0.001), while the cholesterol level by Conelab was significantly lower than Pentra400 (p > 0.001), but not significantly different with Biomajesty (p < 0.05). HDL level by Biomajesty did not differ significantly with Pentra400 (p > 0.05). HDL level by Conelab were significantly lower than Pentra400 (p < 0.001) and Biomajesty (p < 0.01). HDL level by Biomajesty was smallest than Pentra and Conelab. LDL level by Biomajesty did not differ significantly with Pentra400 (p > 0.05) while LDL level by Conelab was significantly lower than Pentra400 (p < 0.001) and Biomajesty (p < 0.001). It can be seen that the range of TG by Biomajesty is smallest (more accurate) than Pentra400 and Conelab. TG level by Biomajesty was significantly lower than Pentra400 (p < 0.01) while TG level by Conelab was not significantly different than Pentra400 (p > 0.05), but it was significantly higher than Biomajesty (p < 0.001).Conclusion and RecommendationThe research shows that Biomajesty is an accurate device for the measurements of HDL, LDL, and TG of icteric samples, whereas Conelab is an accurate device for cholesterol measurement of icteric samples.KeywordsLipid fraction, icteric sample, Pentra, Biomajesty, Conelab
Platelet Count and Platelet Index as Prognosis Markers in Adult Septic Patients Steven Tiro; Raehana Samad; Uleng Bahrun
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1357

Abstract

Sepsis is a major health problem with recent increase of incidence. One of life-threatening complications of sepsis isDisseminated Intravascular Coagulation (DIC). DIC in sepsis can trigger an increase of platelet destruction which can beassessed by platelet count and platelet index. Hence, this research aimed to analyze the correlation of platelet count andplatelet index to the prognosis of adult septic patients. This research was then performed with a retrospective longitudinalstudy design. This research used the medical record data of adult septic patients at the Dr. Wahidin Sudirohusodo GeneralHospital in Makassar. The data of platelet count and platelet index were collected from routine blood of the patients for thefirst time they were diagnosed with sepsis. In total, this research was performed on 100 adult sepstic patients. Fifty of thosepatients had a good prognosis (cured), while the other fifty patients had a poor prognosis (died). Statistical results showedthat the platelet count in the adult sepsis patients with a poor prognosis was significantly lower than those in patients with agood prognosis with median/minimum-maximum of 157,000/12,000-626,000 and 329,000/96,000-801,000, respectively(p=0.00). It was also known that there was no significant difference of MPV values between patients with poor prognosis andpatients with good prognosis with Mean±SD of 9.54±1.44 and 10.08±2.09, respectively (p=0.138). Unlike MPV values, PDWvalues in patients with poor prognosis were significantly higher than those in patients with good prognosis with Mean±SDof 16.7±6.26 and 11.25±2.13, respectively (p=0.00). Thus, it can be concluded that there was an inverse correlation betweenplatelet count and PDW value (r = -0.58). PDW value, as a result, could be used as a prognosis marker for adult septicpatients. However, it was recommended to perform further research as a prospective study by removing the possibility ofbias.
Hyperthyroid Phase of Hashimoto's Thyroiditis Siti Nurul Hapsari; Sidarti Soehita
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1779

Abstract

Hashimoto thyroiditis (chronic autoimmune thyroiditis) is the most common cause of hypothyroidism in iodine-sufficient areas of the world. This condition, however, can sometimes show hyperthyroidism. A 39-year-old femalewas admitted to hospital due to shortness of breath and tremor four hours before hospitalization. There were nausea, chestpain, cold chills, and palpitation. She was diagnosed with Hashimoto's thyroiditis and routinely received tyrosol,propranolol, and dexamethasone. Physical examination showed cervical mass, afebrile, blood pressure of 130/70 mmHg,pulse rate of 110 beats/minute and respiratory rate of 20 breaths/minute. Laboratory examinations showed WBC 7.53 x 109/L, Hb 11.0 g/dL and platelet count of 168 x 109/L. Chest X-Ray: negative for infiltrates. Several laboratory testswere performed, abnormal results were as follows: FT4 level of 2.96 ng/dL (increased), TSH level of 0.003 µIU/mL(decreased), anti-TPO (antithyroid microsomal antibody) level of 306 IU/ml (increased), and IgE level of 213.6 IU/mL(increased). Peripheral blood smear, coagulation test, serum electrolytes, liver function tests, renal function tests, urinalysis,CEA and Ca 125 were within normal limits. Thyroid ultrasound was performed and showed a benign lesion. Fine needleaspiration biopsy showed lymphocytic Hashimoto's thyroiditis. Echocardiography showed hyperthyroid heart disease. Dueto an increase of anti-TPO and FT4 levels, a decrease of TSH levels and lymphocytic thyroiditis from FNAB, this patient wasdiagnosed with a hyperthyroid phase of Hashimoto's thyroiditis. Thyroid function tests and thyroid antibody tests must bemonitored to distinguish between the hyperthyroid and hypothyroid phase of Hashimoto thyroiditis.
Detection of Bacteria Causes Ventilator Associated Pneumonia with Bronchoalveolar Lavage Culture and Endotracheal Aspirate Culture Hairiah Asty; Loesnihari R; Syarani F
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1394

Abstract

Ventilator associated pneumonia (VAP) is the most common hospital infection in ICU. Proper and prompt diagnosis and treatment with adequate antibiotics can reduce the high mortality rate, and prevent complications and antibiotic resistance. Invasive methods, such as bronchoalveolar lavage can make a more accurate diagnosis and help with the choice of antibiotics, but require lung experts. While non-invasive methods, such as endotracheal aspirate can be done faster with less complications. The aim of the study was to determine the pattern of bacterial and bacterial sensitivity to bronchoalveolar lavage and endotracheal fluid. This was an observational study with cross-sectional approaches performed at the Intensive Care Unit of RSUP H. Adam Malik Medan, in August 2017 - February 2018. The sample of  23 patients  who met the criteria and was suspected with VAP was carried out by taking endotracheal aspirate and bronchoalveolar lavage. The samples obtained were culture and sensitivity test using BD Phoenix. There was bacteria pattern had compatibility at moderate levels and there was sensitivity and antibiotic resistance were not significantly different from bronchoalveolar cultures and endotracheal aspirate cultures. Endotracheal aspirate culture has a sensitivity of 78.9% and a specificity of  75% for diagnose VAP. Information on the identification of bacteria and sensitivity testing in patients with suspected VAP is required using appropriate sampling techniques. There were no significant differences between bronchoalveolar lavage culture and endotracheal aspirate culture to diagnosing VAP. Endotracheal aspirate culture is a non invasive diagnostic tool that can be used as an alternative diagnostic tool in patients with suspected VAP.
Author Guideline and Subcribes Form Dian Wahyu Utami
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1778

Abstract

Correlation between WDF, WNR, and RET Abnormal Scattergram Detected by Sysmex XN-1000 and Parasitemia of Malaria Patients in Merauke Hospital Merylin Ranoko; Aryati Aryati; Arifoel Hajat
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1521

Abstract

Malaria remains a health problem in Indonesia. Microscopic examination with Giemsa staining is the gold standard for diagnosing malaria. The density of parasites correlates with the degree of severity and response to therapy of malaria. Malaria-causing plasmodium can be detected by Sysmex XN-1000 which is marked by abnormalities in the WDF, WNR and RET scattergram. This research aimed to determine the correlation of WDF, WNR and RET abnormal scattergram detected by Sysmex XN-1000 and the parasitemia index of malaria at the Merauke General Hospital. This was a cross-sectional study with observational approach conducted between November 2017 – February 2018 at the Merauke General Hospital. Positive malaria samples were stained with Giemsa, their parasitemia index was calculated, routine complete blood count using Sysmex XN-1000 was performed, and the scattergram abnormalities were then analyzed. There were 65 positive malaria samples as follows: P.falciparum (35%), P.vivax (60%), P.ovale (3.1%), and P.malariae (1.5%), but the species did not correlate with parasitemic index (p=0.691). Abnormalities of WDF and WNR scattergram were predominantly found than RET scattergram (80% vs. 27.7%). P.vivax predominantly caused abnormalities of the WDF and WNR scattergram in 36 of 39 samples (92.3%), whereas P.falciparum predominantly caused abnomalities of the RET scattergram in 14 of 23 samples (60.9%). There was 95% positivity of an abnormality in WDF/WNR/RET scattergram with a cut-off of > 5,0165.5/µL. There was correlation between WDF, WNR, RET scattergram detected by Sysmex XN-1000 and the parasitemia index.

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