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CORD
Published by International Coconut Community
ISSN : 02151162     EISSN : 27218856     DOI : 10.37833/cord
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Library & Information Science
Aims CORD aims to publish original research results and reviews on research and development. While encouraging those involved in research and developments to disseminate their finding it also assists policy makers and planners to be aware of the latest developments in the sector Scope CORD encompasses a broad range of research topics in animal sciences: seedlings and genetics, farming, harvesting, production, health, biotechnology, and production, socio-economic, and policy.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol 27 No 2 (2011): CORD" : 8 Documents clear
Development of Indian Breakfast Foods with Coconut Flour Ramaswamy, Lalitha; Sivagami, A. R
International Coconut Community Journal Vol 27 No 2 (2011): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (663.616 KB) | DOI: 10.37833/cord.v27i2.111

Abstract

Coconut flour was incorporated in five Indian breakfast foods at 10%, 20% and 30% level by replacing the major cereal of each dish and organoleptically evaluated. Adai made with 10% incorporation had obtained the maximum scores, while those made with 20% and 30% were hard and rubbery. Uppuma made with 10% incorporation was almost equal to that of standard while the 30% incorporated one was unfit for consumption. Similarly mooli paratha made with 30% incorporation was crisp, however with 10% incorporation they were soft and acceptable. Vermicelli kichadi made with 30% incorporation was sticky, while the variation made with 10% was acceptable. Poories made with 10% incorporation were acceptable and the flavour was desirable due to the unique coconut flavour, however as the proportion of coconut flour increased the poories became crisper. Results of ANOVA show that there is a significant difference between the variations on account of coconut flour incorporation and with the standard. The fibre content of the most acceptable recipes was 4g more, while the carbohydrate and fat content were less than the standard which helps in combating disease conditions like obesity and diabetes mellitus. Therefore novel foods can be formulated with coconut flour and popularised.
Use of Arabinogalactan Protein in Coconut (Cocos Nucifera L.) Tissue Culture: An Alternative Approach for Improved Tissue Response Fernando, S. C.; Samarajeewa, D. A. D. S.; Liyanagedara, L. G. N. H. K.
International Coconut Community Journal Vol 27 No 2 (2011): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.414 KB) | DOI: 10.37833/cord.v27i2.112

Abstract

Arabinogalactan proteins (AGPs) are extra-cellular proteins involved in plant growth and development. The effects of these proteins on in vitro response of different species are well documented. This study assessed for the first time the role of AGPs on tissue culture of coconut, a highly recalcitrant species in vitro. Coconut (Sri Lanka Tall) plumules were cultured in medium containing 2, 4-dichlorophenoxyaceticacid (2,4-D) for callogenesis. Calli were multiplied by subculturing onto freshly prepared callusing medium. Somatic embryos were induced by transferring of calli to medium with 2/3 concentration of initial 2,4-D and matured by subsequent transferring to medium without any hormones. Embryos were converted in the presence of gibberelic acid. Arabic gum (1-50 mgL-1) was used as the main source of AGP. Alternatively, different AGP sources like Larch wood gum, carrot seeds and defatted coconut kernel were also assessed. Arabic gum in callusing medium resulted in early callogenesis from 20-40% plumules compared to 10% in the control. However, it did not have a significant effect on final callusing efficiency. Its presence in somatic embryogenesis medium showed a significantly higher mass of embryogenic structures per an embryogenic clump (33.3-50.5mg) when compared to the control (26.9mg).The best result was obtained at 10mgL-1 Arabic gum. All AGP sources except Larch wood gum had positive effects on somatic embryogenesis. Among them, defatted coconut kernel (25-50mgL-1) showed the best results giving 70% cultures with embryogenic structures compared to 37% in control. However, so far, the positive effect of AGP did not reflect in plant regeneration efficiency.
Bioinformatic Prediction of SNP Markers in WRKY Sequences of Palms Manju, K. P.; Arunachalam, V.
International Coconut Community Journal Vol 27 No 2 (2011): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.598 KB) | DOI: 10.37833/cord.v27i2.113

Abstract

WRKY transcription factors are unique to plants and performs many imperative functions mainly disease resistance. In the present study we have analyzed the WRKY transcription factor gene sequences to assess the variation at single nucleotide level. We have retrieved 525 sequences of WRKY genes of palms of 334 Kb size. The sequences were purified by employing EST trimmer and were clustered into 31 contigs using CAP3. Single nucleotide Polymorphisms (SNP) and insertion/deletions (indels) were detected in contigs using the AUTOSNP software. Alternatively candidate SNP containing contigs were aligned by Clustal X to locate the SNPs. Results from these two methods were compared and false SNPs were eliminated. Finally, about 568 SNPs were found including 250 transitions, 120 transversions and 198 indels. The SNPs were seen at a frequency of 2.84/100bp in the WRKY sequences of palms. Primers were designed flanking to SNP/ indel sites with potential as markers in palms. We could obtain two novel WRKY-SNP markers (WRKY 7 and WRKY 12) which are not reported before in palms.
Effect of Gelling Agent and Selective Sub-culturing on Hyperhydricity in Anther-Derived Coconut Embryos Perera, P. I. P.; Kularatene, J. D. J. S.; Weerakoon, L. K.
International Coconut Community Journal Vol 27 No 2 (2011): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.811 KB) | DOI: 10.37833/cord.v27i2.114

Abstract

Experiments were conducted to compare the liquid medium with the media solidified with agar or phytagel. Selective subculturing and use of the embryo maturation medium supplemented with higher concentration of phytagel (0.5%; w/v) were also tested for reducing the vitrified embryos. Modified Eeuwens Y3 medium was used as the basal medium. By culturing the anthers on the medium solidified with phytagel (0.25%; w/v), direct embryo formation (86.7%) and embryo conversion (21.5%) were significantly increased. Plant regeneration efficiency of anther derived embryos or calluses developed in the liquid culture medium was extremely low (2.4%). Vitrification was further reduced by incorporating 0.5% (w/v) phytagel into the embryo maturation medium. Highest plant regeneration efficiency was obtained by exposing the embryos to 0.5% (w/v) phytagel for 21 days, which reduced vitrification by 42%. Furthermore, selective subculturing of the embryos was effective for reducing vitrification.
Effect of Application Frequency of the Entomopathogenic Fungus Hirsutellathompsonii (Fisher) on Damage Levels of Coconut Mite Aceriaguerreronis (Keifer) Fernando, L. C. P.; Hapuarachchi, D. C. L.; Waidyarathne, K. P.
International Coconut Community Journal Vol 27 No 2 (2011): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (538.58 KB) | DOI: 10.37833/cord.v27i2.115

Abstract

The effect of application interval of H. thompsonii (Fisher) on damage caused by the coconut mite, Aceriaguerreronis (Keifer) (Acari: Eriophyidae) in two coconut plantations in Sri Lanka was evaluated. In each plantation, coconut palms infested by coconut mite were treated with H. thompsonii isolate IMI 391722 at 2- and 3-monthly intervals and the fruits with H. thompsonii-infected coconut mite cadavers and percentage of harvested fruits in different damage categories were assessed. Infected coconut mite cadavers were found on up to 60% of the fruits treated with the fungus in both plantations at treatment frequencies. The effect of the treatments was site-specific. The treatments reduced the percentage of damaged-small size harvested fruits to half that of the untreated control in both plantations irrespective of the frequency of treatment. At one plantation, the percentages of undamaged fruits and fruits with discontinued damage were nearly doubled to that of untreated fruits.
Azolla & Soya Hulls-Substitutes for Urea in Coir Pith Composting Using Pleurotus Sajor Caju Radhakrishnan, S.; Ravindranath, Anita Das; Sarma, U. S.; Jayakumaran, Nair A.
International Coconut Community Journal Vol 27 No 2 (2011): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1295.499 KB) | DOI: 10.37833/cord.v27i2.116

Abstract

Bio composting process is the available means of converting various organic wastes generated from the industry and the agricultural sectors into beneficial products such as biofertilizers and as a soil conditioners. The unique feature of these agricultural wastes are their organic content, pH, and high C: N ratio. Coir pith is a biomass residue obtained during the extraction of coir fibre from coconut husk. Accumulation of coir pith leads to an environmental concern and its management is a major problem with all coir industrialists. The high quantum of its production and the difficulties experienced in its disposal have tended to create a major problem of pollution of large areas of land and water in coir fiber extraction units. Therefore, biodegradation of coir pith is an essential requirement to control pollution. Coir pith, even though a problematic waste; it is a potential wealth when it is converted into valuable organic manure by microbial degradation. A method of composting of coir pith with urea has been developed by Coir Board, India using a fungus viz., Pleurotus sajor caju commercially known as Pithplus. Application of urea releases higher concentration of ammonia in the soil making it more acidic and retards soil natural fertility. Hence an alternative cost effective nitrogen supplement needs to be developed as a substitute to urea for composting of coir pith. The present study aims at the formulation of bio organic manure from coir pith avoiding inorganic urea in the composting. Overall assessment on the physico chemical properties of the biodegraded coir pith leads to the conclusion that a combination of Azolla and Soya hulls (2:1 ratio) was found to be efficient in lignin degradation and contribute to novel changes in coir pith
Effect of Rock Phosphate on Nutrient Status and Nut Load of Mature Coconut in South-Western Ghana Andoh-Mensah, E.; Addae-Manu, A.; Nutsukpo, D. K.
International Coconut Community Journal Vol 27 No 2 (2011): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.041 KB) | DOI: 10.37833/cord.v27i2.117

Abstract

The effect of rock phosphate on mature coconut was evaluated from 2004 to 2010 within the context of low nut yield due to phosphorus deficiency in the acidic soils of South-Western Ghana. A low reactive Togo rock phosphate containing 25.5% P205 was applied. Three application schedules based on 666 kg/ha rock phosphate and a control were evaluated in a Randomized Complete Block Design with five replications. The application schedules were: (a) Bulk application at 4.5 kg/tree (b) Two-equal-split application at 2.25 kg/tree in years 1and 3 (c) Three-equal-split application at 1.5 kg/ tree in years 1, 2 and 3. Muriate of potash fertilizer was applied at 2 kg/tree as basal treatment. Plot size was 0.2 ha comprising 30 trees. Nut load and leaf nutrient status of coconut palms were determined. Rock phosphate led to a significant (P<0.01) increase in leaf phosphorus and nut yield. It sustained a superior nut load for a 4-year period beginning from year 2 of application to year 5 with average nut gain of 19.0 per tree compared to 4.6 per tree in the control palms. There was no significant (P>0.05) difference in nut yield and nutrient status between bulk and split methods of rock phosphate application. A value-to-cost ratio of 1.5 was obtained thus indicating the profitability of rock phosphate application in the acidic soils of South-Western Ghana. Coir geotextiles find application in revegetation of slopes by stabilizing the soil through erosion control. It has been found that the longevity of coir geotextiles although highest among all the natural fibres, it is required to last for at least 5 years so as to sustain the vegetation on the slopes for a long term solution. Normally it is found that coir geotextiles lose their 50% strength in 6 months in contact with soil, therefore it is required to strengthen the coir geotextiles.
Coir Pith as Growth Medium for Azotobacter Vinelandii and Azospirillum Brasilense Reghuvaran, Abesh; Ravindranath, Anita Das
International Coconut Community Journal Vol 27 No 2 (2011): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.902 KB) | DOI: 10.37833/cord.v27i2.118

Abstract

Coir pith is a lignocellulosic biomass which is recalcitrant under ordinary conditions. Nitrogen fixation is commonly carried out in the soil and these soils acts as the medium for plant growth. This paper attempts to utilize coir pith as a substrate for two important nitrogen fixing organisms viz. Azotobacter vinelandii and Azospirillum brasilense. Coir pith was used as a source of carbon and energy by the bacteria and the ammonia produced during the process of nitrogen fixation was studied, the amount of ammonia produce indicates the fixation process by the bacteria. The present work succeeded in establishing the use of these two organisms to degrade the coir pith effectively and the resultant biodegraded material could be used as organic manure for plants.

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