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3BIO: Journal of Biological Science, Technology and Management
Published by Institut Teknologi Bandung
ISSN : -     EISSN : 26558777     DOI : http://dx.doi.org/10.5614%2F3bio.2020.2.1
Core Subject : Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Immunology & microbiology Neuroscience
3Bio: Journal of Biological Science, Technology and Management is an interdisciplinary peer-reviewed journal in a wide aspect related to the field of life sciences and other related fields of study. The journal aims to promote scientific discourse and disseminate research on various branches and applications of bio-science, biotechnology and bio-based management. This journal invites original empirical research, literature reviews, theoretical or methodological contributions, or short communications on, but not limited to, the following topics: - Ecology and Biosystematics - Microbiology - Genetics and Molecular Biology - Animal Development and Physiology - Plant Development and Physiology - Entomology - Biomedical science - Biochemistry - Agronomy - Forestry - Bioengineering - Bioethics - Management of Biological Resources The journal also invites contributions from other associated disciplines. This journal is an open-access journal. Readers may read, download, copy, distribute, print, search, or link to the full texts of these articles without any charge, provided that readers acknowledge the Creative Commons attached to the articles. All submitted papers are reviewed by at least two referees before being accepted for publication, following a double-blind review process. This journal is delivered in an academic English and rigorously edited to provide a quality standard of a scientific journal. This journal is managed by the School of Life Sciences and Technology, Institut Teknologi Bandung, Indonesia.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
 1 
Search results for , issue "Vol. 2 No. 1 (2020)" : 5 Documents clear
The effect of Paraquat Dichloride application on diversity and abundance of soil arthropods in the corn field Tjandra Anggraeni; T Taufikurahman; M Meilisa; Yeyet Setiawati; Isma Fadilla; Asih Suryati
3BIO: Journal of Biological Science, Technology and Management Vol. 2 No. 1 (2020)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2020.2.1.1

Abstract

We investigated the effect of paraquat dichloride herbicide application on diversity and abundance of soil arthropods in a corn field. In addition to control (0 mL/L), four concentrations of paraquat dichloride were applied i.e. 3.33 mL / L, 4 mL / L, 4.66 mL / L and  5.33 mL / L. Sampling was carried out four times, namely at T0 (before tillage), T1 (after tillage), T2 (after spraying herbicides), and T3 (before harvesting) using a pitfall trap method. Soil arthropod samples were identified to the family level. The total number of individuals was then analyzed to obtain species richness, species dominance index, species diversity index and similarity index of Sorensen. The result showed that paraquat dichloride did not give any significant effect (p>0.05) on the number of individuals, species richness, species dominance index, diversity index and Sorensen similarity index of soil arthropods. The composition of soil arthropods in the control and in the treatment with the highest concentration shows a high level of similarity.
Chemical content in Two Teak Woods (Tectona grandis Linn.F.) that has been used for 2 years and 60 years Enih Rosamah; Fera Ferliyanti; Harlinda Kuspradini; Rudi Dungani; Pingkan Aditiawati
3BIO: Journal of Biological Science, Technology and Management Vol. 2 No. 1 (2020)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2020.2.1.3

Abstract

Teak (Tectona grandis Linn F.) is classified as luxury wood and belongs to the durable wood, resistant to termite and fungal attacks. The purposes of this study were to analyze and compare the chemical content and bioactive compound of teak (T. grandis Linn F.) from Sumedang, West Java based on age of use. This study used teak woods of 2 and 60 years of use. The chemical components analyzed by determination of lignin, extractives, and ash content. Phytochemical compounds were analyzed by color changing of crude acetone extracts. The results showed that the teak wood with 2 years of use possessed lignin of 28.41%; cold water soluble extractives of 4.26%; hot water soluble extract of 5.12%; NaOH 1% soluble extractives of 19, 40; and alcohol:benzene (1:2) soluble extract of 6.21%; while ash content of  0.85%. Meanwhile teak wood after use of 60 years showed the lignin content of 29.82%; cold water  extract of 1.56%; hot water  extract of 2.56%; 1% NaOH soluble extract of 12.30%;  alcohol:benzene (1:2) extract of 4.62%; ash content of 1.36%. The qualitative phytochemical test demonstrated both of teak wood after use of 2 years and use of 60 years contained flavonoids, tannins, triterpenoids, cumarins, and carbohydrates.
A Comparison of Alkali and Biological Pretreatment Methods in Napier Grass (Pennisetum purpureum Scumach.) for Reducing Lignin Content in the Bioethanol Production Process T Taufikurahman; Wendo Obert Delimanto
3BIO: Journal of Biological Science, Technology and Management Vol. 2 No. 1 (2020)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2020.2.1.5

Abstract

Napier grass is one of lignocellulosic plants that has the potential to be converted as bioethanol due to high productivity and relatively fast harvesting time. However, the problems of processing lignocellulosic plants into bioethanol are the high lignin content and the different lignin structure of each plant. Lignin can inhibit biological agents in accessing cellulose and hemicellulose. Therefore, it is necessary to select and optimize the pretreatment process with the aim of degrading lignin and maintaining the value of the cellulose. This study was conducted to compare the effectiveness of two different pretreatments (biological and alkaline) in degrading lignin. For the alkali pretreatment, lime (Ca(OH)2) was added to the Napier grass substrate using concentrations of 0.05, 0.1, and 0.5 grams/gram, which was then incubated at 23-25 oC each within 6, 24, and 96 hours period. For biological pretreatment, Aspergillus niger spore was used as an agent, which was incubated onto the Napier grass substrate using concentrations of 106, 107, and 108 cells/mL, an optimal temperature of 35oC and within 1, 3, 5, 7, and 9 days period. As a comparison, Phanerochaete chrysosporium was also incubated using a concentration of 106 a temperature of 35 oC within 28 days period. The extracted Napier grass was then analyzed for lignocellulose content, which included hot water soluble, Hemicellulose, cellulose, lignin, and ash, using Chesson-Datta method and reducing sugar test. Comparison of biological pretreatment between Aspergillus niger and Phanerochaete chrysosporium showed that Aspergillus niger was better at degrading lignin, with a lignin-to-cellulose ratio of 24.3%, smaller than Phanerochaete chrysosporium at 30.645%. This ratio was furthermore compared with the ratio resulting from Alkali pretreatment, which showed that the former was proven to be more optimum.
Bioconversion of Napier Grass Mixed with Soybean Curd Residue Producing Bioethanol through Simultaneous Saccharification and Fermentation in a Solid State Culture T Taufikurahman; Jessica Jessica
3BIO: Journal of Biological Science, Technology and Management Vol. 2 No. 1 (2020)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2020.2.1.4

Abstract

Napier grass is a promising lignocellulosic biomass for bioethanol production because of its high cellulose content and high annual productivity. Converting a lignocellulosic biomass into a bioethanol usually takes two steps which resulted in a long processing time and sometimes includes the utilization of hazardous chemicals. Simultaneous saccharification and fermentation in a solid-state culture using single bioconversion agent, Neurospora sitophila Shear, can reduce the overall processing time and also increase the yield of the products. The research is aimed to determine the optimum aerobic and micro-aerobic conditions that yields the highest enzyme activity and bioethanol concentration from the mixture of Napier grass and soybean curd residue. The saccharification and fermentation process was conducted in the laboratory using an incubator at 33oC. The cellulase enzyme activity was calculated as FPAse. The highest activity achieved was 0.538 filter paper unit with the optimum mass ratio of Napier grass to soybean curd residue was 1:1 on the second day of cultivation period. The fermentation process was conducted aerobically for two days and then followed by six days of micro-aerobic fermentation, resulting in the highest bioethanol yield of 2.12% (w/w) at the end of the culture period. The optimum mass ratio was found to be 9:1. This study shows that Napier grass and simultaneous saccharification and fermentation method has a great potential for cellulase and bioethanol production, but further improvement on the micro-aerobic system is needed to maximize the bioethanol yield.
The Physiological Responses of Water Hyacinth (Eichhornia crassipes (Mart). Solms) and Water Lettuce (Pistia stratiotes L.) as Trivalent Chromium Bioaccumulator Rizka Purnamawati; T Taufikurahman; Andira Rahmawati; Chalvin Rura Putra; Din Dzakamala Fafi Rahmatilah; Findy Ashgi
3BIO: Journal of Biological Science, Technology and Management Vol. 2 No. 1 (2020)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2020.2.1.2

Abstract

Chromium is one of the heavy metals used in industrial fields, i.e., metallurgical industry, chemical industry, heat-retardant, and leather tanning industry. Untreated wastewater from these industries can pollute rivers and threaten the aquatic ecosystem. Some aquatic plants such as water hyacinth (Eichhornia crassipes) and water lettuce (Pistia stratiotes) have been known as metal hyperaccumulators and can be used as phytoremediator for polluted water. This study aims to determine the physiological and morphological responses of water hyacinth and water lettuce plants treated by different concentrations  of trivalent chromium and to study which plant is effective for trivalent chromium removal. The experiment was conducted for 2 week at screen house. After two weeks, the plants were harvested and the weight was measured. The samples were separated into shoot and root and were analyzed for Cr content, chlorophyll, proline and CAT enzyme activity. The results of this study indicated that the levels of water hyacinth chromium in roots and leaves are lower (863.988 mg/Kg, 899.126 mg/Kg, 685.877 mg/Kg for treatment 40 ppm, 80 ppm, and 120 ppm respectively) than the levels of chromium in water lettuce (1584.264 mg/Kg, 1660 mg/Kg, 1413 mg/Kg for treatment 40 ppm, 80 ppm, and 120 ppm respectively). Physiological parameter, i.e., chlorophyll and proline levels in water hyacinth and water lettuce from all concentration treatment did not differ significantly (P> 0.05). The activity of the catalase enzyme in water hyacinth and water lettuce decreased with increasing chromium levels. The highest catalase enzyme activity was observed in control treatment of water lettuce (1.61 unit/mg) and 40 ppm treatment of water hyacinth (1.006 unit/mg). The highest biomass dry weight of both plants was found in plants with control treatment (15.38 gr and 8.48 gr for water hyacinth and water lettuce respectively). Therefore, we concluded that water lettuce is better for trivalent chromium removal than water hyacinth.

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