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Jurnal Ilmiah Biologi UMA (JIBIOMA)
Published by Universitas Medan Area
ISSN : -     EISSN : 27229777     DOI : 10.31289
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Ilmiah Biologi UMA (JIBIOMA) focuses on the publication of the results of scientific research related to fields. This article is published in the internal and external academic community of the University of Medan Area (UMA) especially in the field of Biology. Published articles are the results of research articles, studies or critical and comprehensive scientific studies on important issues related to the field of biology. Publication in May and November.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 14 Documents
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Search results for , issue "Vol 6, No 2 (2024): November" : 14 Documents clear
Isolasi Bakteri Pencemar pada Jajanan Tradisional di Kawasan Jalan Al-Falah Ardepy, Sri; Sartini, Sartini; Rahmiati, Rahmiati; Siregar, Rahma Sari
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 6, No 2 (2024): November
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v6i2.5176

Abstract

Traditional snack cakes can be contaminated with harmful microbes due to poor hygiene and sanitation practices. This study aims to identify the types of bacteria contaminating traditional snacks sold by street vendors on Jl. Al-Falah. The method used is the pour plate technique on Plate Count Agar for 24 hours at a temperature of 33-35 °C, along with selective media Salmonella Shigella Agar and Eosin Methylene Blue Agar. Observations include both fresh samples and those stored for 24 hours. Confirmation tests were conducted on SSA and EMBA media, followed by SCA (Simmon Citrate Agar). The results showed that six types of cakes exceeded the microbial contamination limits: P1S1 (6.2 × 10⁵ colonies/g), P2S1 (10 × 10⁴ colonies/g), P3S1 (4.8 × 10⁴ colonies/g), P1S5 (5.6 × 10⁴ colonies/g), P2S5 (5 × 10⁴ colonies/g), and P3S5 (8.6 × 10⁴ colonies/g). Out of 15 samples, two were detected to contain pathogenic bacteria: P1S1 was positive for Salmonella sp. and Escherichia coli, while P3S2 was positive for Escherichia coli. In conclusion, the contaminating bacteria Salmonella sp. and Escherichia coli were found in cake samples from different vendors.
Identifikasi Efektivitas Lichen Crustose sp. (Cryptochemia) dan Lichen Foliose sp. (Parmelia) Sebagai Bioindikator Tingkat Pencemaran Udara Nisa, Alyyah Fikrotun; Rosdiana, Laily; Roqobih, Fikky Dian
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 6, No 2 (2024): November
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v6i2.4352

Abstract

Air pollution is still an environmental problem that has not been resolved until now. There are several alternative methods that can be applied to monitor air quality levels, one of which is using bioindicators. Lichens can be used as bioindicators because they are accumulators and are very adaptive to changes in environmental conditions. This study aims to assess the effectiveness of Crustose sp. (Cryptochemua) and Foliose sp. (Parmelia) lichens as bioindicators of air pollution levels. This study used a systematic review method. Literature was selected based on 3 criteria: relevant to the topic, journal articles within 5 years, and from SCOPUS or SINTA accredited journals. After the selection process, 6 articles were selected for review based on the specified criteria. The results showed that to identify Crustose sp. (Cryptochemua) and Foliose sp. (Parmelia) lichens as bioindicators of air pollution, it is not enough to only consider the number of lichens or morphology found. However, it is also necessary to identify the affected lichen species, biotic and abiotic factors in order to obtain effective and valid results. For future researchers, it is recommended to compare the efficiency of Crustose sp. and Foliose sp. lichen plants as bioindicators of air pollution with other plants.
Kultur Embrio Mencit In Vitro: Studi Perbandingan Efektifitas Media M16 dan CZB Foenna, Yudi Gebri; Nasution, Jamilah
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 6, No 2 (2024): November
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v6i2.5284

Abstract

Successful embryo culture is influenced by culture methods and conditions, which have an impact on cytoplasmic factors and the ability of embryo development. This study aims to compare the effectiveness of two culture mediums, M16 and CZB, in supporting the development of DDY strain mice embryos in vitro. The research method used was an experimental study consisting of animal husbandry, medium culture preparation, superovulation, collection, and in vitro embryo culture. Data analysis in this study used descriptive statistical tests to measure embryo development in each culture medium, M16 and CZB, in observations for 96 hours with a 24-hour interval using an inverted microscope. The results showed that embryos in the CZB medium experienced 50% development of the expanded blastocyte stage after 96 hours of culture with 50% degenerative. In the M16 culture medium, 0% of embryos experienced embryonic stage development after 96 hours of culture and 100% were degenerative. This study has concluded that the development of embryos in vitro in the M16 culture medium was not better than CZB, this was evidenced by the percentage at the expanded blastocyte development stage.
Analisis Kontaminasi Bakteri Patogen pada Sayuran yang Dijual di Pasar Tradisional Kota Medan dengan Media EMBA dan SSA Lumbar Raja, Doan Parulian; Rahmiati, Rahmiati; Susilo, Ferdinand; Nasution, Jamilah
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 6, No 2 (2024): November
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v6i2.5175

Abstract

Microbial contamination in vegetables often originates from spraying or irrigation with contaminated water. Fertilization using animal manure can also lead to contamination, with bacteria such as Salmonella and E. coli found in vegetables like lettuce. Samples were collected once from each traditional market, repeatedly taken from roadside stalls, inside the market, or from kiosks. Macroscopic colony characteristics on EMBA (Eosin Methylene Blue Agar) and SSA (Salmonella-Shigella Agar) media were observed after incubation, including colony shape, color, structure, and growth size or diameter. EMBA media cultures of E. coli on five vegetable samples from Pasar Melati, Pasar Sei Sikambing, and Pasar Kampung Lalang showed contamination in all five samples. SSA media cultures of Salmonella sp. on five vegetable samples from these markets revealed contamination in one sample. Gram staining of all samples identified Gram-negative bacteria, appearing red in color.

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