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Dr. Charis Amarantini, M.Si
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Fakultas Bioteknologi Universitas Kristen Duta Wacana Yogyakarta Jl.Wahidin Sudirohusodo No.5-25 Yogyakarta 55224 Tel : 0274-563929 Ext.459 Fax : 0274 520842
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Sciscitatio : Journal for Biological Science
Published by Universitas Kristen Duta Wacana
ISSN : -     EISSN : 27215180     DOI : https://doi.org/10.21460/sciscitatio.2020
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Sciscitatio is dedicated to the publication of original research articles in basic biology and biotechnology fields including environmental biotechnology, industrial biotechnology, medical biotechnology and bioentrepreneurship. Sciscitatio is published twice a year in January and July, and intended to be a critical scientific forum for the application of biology to solve recent and up-to-date issue in the society. Sciscitatio adopted an open access policy.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 6 Documents
 1 
Search results for , issue "Vol 1 No 2 (2020): Volume 1, Number 2, July 2020" : 6 Documents clear
Community Perception Surrounding Riung National Park to the Conservation of Komodo Dragon Blegur, Willem Amu; Djohan, Tjut Sugandawati; Ritohardoyo, Su
SCISCITATIO Vol 1 No 2 (2020): Volume 1, Number 2, July 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.12.29

Abstract

People on Benteng Tengah, Nangamese and Latung have been living in Flores Island and smaller island nearby Flores, like Ontoloe, long before the establishment of national park. In 1992 and 1996, the government established Nature Conservation of Wolo Tado, Nature Conservation of Riung and Marine Nature Conservation of 17 Pulau. This decision led to government’s policy to prohibit the opening of land by burning. Local people used to open a land for agricultural purpose by setting a fire in order to regenerate the savanna to promoting the growth of young grass leaves. People use young grass to feed their cattle. This prohibition causes the people had to herd their cattle far from they live. As the consequence, threat from Komodo (Varanus komodoensis) to attack cattle is increased and people consider Komodo as pest that has to be terminated. This research aimed to study people’s knowledge about Komodo status as endangered species and its implication. Data were collected from people who lives in Benteng Tengah, Nangamese, and Latung, Regency of Ngada, East Nusa Tenggara. Data were obtained from respondents using interviews and questionnaires. Perception of local people who lives in Benteng Tengah (93%), Nangamese (93%) and Latung (100%) showed that people are aware about Komodo’s habitat vegetation. Good perception on Komodo and habitat vegetation will maintain komodo sustainability.
Toksisitas Akut Ekstrak Etanol Daun Rumput Knop (Hyptis capitata Jacq.) dengan Metode Brine Shrimp Lethality Test (BSLT) To'bungan, Nelsiani; Jati, Wibowo Nugroho; Zahida, Felicia
SCISCITATIO Vol 1 No 2 (2020): Volume 1, Number 2, July 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.12.30

Abstract

Informasi mengenai keamanan penggunaan rumput Knop (Hyptis capitata Jacq.) sebagai obat tradisionalmasih terbatas. Uji keamanan melalui uji toksisitas penting untuk dilakukan sebelum dilakukan uji manfaatlebih lanjut. Salah satu uji toksisitas akut yang dapat dilakukan sebagai uji toksisitas tahap awal adalah ujitoksisitas akut dengan metode Brine Shrimp Lethality Test (BSLT). Daun rumput Knop diekstrak dengan penyarietanol dengan metode maserasi. Ekstrak etanol daun rumput Knop dengan konsentrasi 1000, 500, 250, 125 dan62,5 µg/ml dipaparkan pada larva Artemia salina (L.) selama 24 jam. Tingkat toksisitas ditentukan berdasarkannilai LC50 yang diperoleh berdasarkan jumlah larva yang mati, melalui analisis probit. Kandungan fitokimiaekstrak etanol daun rumput Knop diuji melalui uji fitokimia kualitatif. Nilai LC50 ekstrak etanol daun rumputKnop sebesar 183,91 µg/ml. Ekstrak etanol daun rumput Knop mengandung alkaloid dan steroid.
Kandungan Logam Berat Timbal (Pb) pada Ikan Nila (Oreochromis niloticus) dan Ikan Bawal (Colossoma macropomum) di Sungai Winongo, Yogyakarta Purwanto, Annabelle Indryana; Prihatmo, Guruh; Pakpahan, Suhendra
SCISCITATIO Vol 1 No 2 (2020): Volume 1, Number 2, July 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.12.31

Abstract

Sungai Winongo memiliki peran cukup penting di masyarakat kota Yogyakarta. Penelitian ini bertujuanuntuk mengetahui konsentrasi Pb ada air, sedimen (batu, kerikil, pasir dan lempung) dan tiga organ ikan Nila(Oreochromis niloticus) serta ikan Bawal (Colossoma macropomum) yaitu organ viscera, tulang dan daging. Sampeldiperoleh dari tiga titik yaitu bagian hulu, tengah dan hilir. Total 120 sampel diekstraksi menggunakan metodeaqua regia digestible dengan perbandingan larutan HCl : HNO3(3:1, v/v) dan ekstrak dianalisis menggunakanAtomic Absorption Spectrophotometry (AAS). Ditemukan rata-rata konsentrasi Pb pada air (0,054 mg/L), sedimen(38,29 mg/kg), ikan Nila ( viscera 11,35 mg/kg; tulang 10,28 mg/kg; daging 6,46 mg/kg ), dan ikan Bawal (viscera 5,31 mg/kg; 9,85 mg/kg; daging 5,23 mg/kg ). Konsentrasi Pb pada air telah melebihi batas baku mutuPPRI No. 82 Th 2001 (0,03mg/L). Konsentrasi pada ketiga bagian ikan Nila dan Bawal yang diteliti (viscera,tulang, daging) telah melebihi batas baku mutu yang ditetapkan oleh SNI 7387:2009 (0,3 mg/kg).
Potency of Citrus reticulata Peel Extract as Active Compound of NonAlcohol Based Gel Hand Sanitizer Santoso, Julyana Dika; Prasetyaningsih, Aniek; Madyaningrana, Kukuh
SCISCITATIO Vol 1 No 2 (2020): Volume 1, Number 2, July 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.12.32

Abstract

Hand sanitizer is nowadays known as a part of one’s personal hygiene kit because of its practical use andeffectivity against skin microbes, such as Staphylococcus aureus. The common commercial hand sanitizers are madefrom alcohol which may have negative side effect like skin irritation. Utilization of active compounds from a plantthat has antibacterial compounds might be applied to the substitution of alcohol in the formulation of hand sanitizer.In this study, phytochemical compounds from Citrus reticulata and Citrus aurantifolia peel extracts were testedqualitatively and their antibacterial acitivity on Staphylococcus aureus were tested using disk diffusion method. Theresults showed that Citrus reticulata peel extract at the concentration of 70% was the most effective concentration ininhibiting Staphylococcus aureus. This certain concentration of Citrus reticulata peel extract was then chosen in the gelhand sanitizer formulation. Application of gel hand sanitizer with Citrus reticulata peel extract as its active compoundon the hands of respondents were also observed to inhibit bacterial growth. The use of peel extracts from Citrusspp, especially from Citrus reticulata might be potential in the formulation of non-alcohol based gel hand sanitizer.
Isolation and Identification of Biofilm-Forming Staphylococcus Aureus in Commercial Cow Milk Products Yambise, Dominggas; Ariestanti, Catarina Aprilia; Budiarso, Tri Yahya
SCISCITATIO Vol 1 No 2 (2020): Volume 1, Number 2, July 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.12.33

Abstract

Food poisoning caused by the contamination from Staphylococcus aureus are frequently found in foodespecially in dairy products. Pasteurization process in milk production was not enough to kill S. aureus becauseit formed biofilm that could survive in high temperature. This research aimed to study the presence of biofilmforming S. aureus in samples from packed commercial milk products in Yogyakarta City, Indonesia. Twentyisolates from dairy products were grown in Brain Heart Infusion (BHI) broth then inoculated into Braid-ParkerAgar (BPA) medium to get the candidate of S. aureus isolates. These isolate candidates were selected usingMannitol Salt Agar (MSA) and Congo Red Agar (CRA) medium. Another selection was done by carbohydratefermentation analysis and confirmed using API STAPH. Confirmation analysis showed that eight isolateswere identified as S. aureus. Another two isolates were identified as S. xylosus and S. haemolyticus. Therefore,it indicated the presence of Staphylococcus aureus as contaminant in dairy products.
Aktivitas Hidrolisis Ekstrak Kasar Lipase dari Kecambah Biji Kesambi (Schleichera oleosa L) dengan Variasi Waktu Perkecambahan Hendrik, Gersandi; Ledo, Mellissa Erlyn Stephanie; Nitsae, Merpiseldin
SCISCITATIO Vol 1 No 2 (2020): Volume 1, Number 2, July 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.12.34

Abstract

Biji kesambi mengandung beberapa jenis asam lemak seperti asam miristat, asam palmitat, asam stearat,asam arakidat, asam oleat dan asam linoleat. Proses perkecambahan merupakan tahap awal perkembangantumbuhan berbiji yang didukung oleh peran enzim. Enzim lipase berperan untuk mengatur kecepatanpemecahan lemak dan sintesis lemak. Tujuan penelitian ini adalah untuk mengetahui aktivitas hidrolisis ekstrakkasar lipase kecambah biji kesambi dan kadar protein dalam ekstrak kasar lipase kecambah biji kesambi. Hasilpenelitian ini menunjukan aktivitas hidrolisis ekstrak kasar lipase kecambah biji kesambi tertinggi adalah harike 13 sebesar 41,3 U/ml, kadar protein tertinggi pada hari ke 13 sebesar 1,11 gr/mL, aktivitas spesifik tertinggipada hari ke 11 sebesar 69,20 U/gr, sehingga penggunaan kecambah biji kesambi pada usia kecambah 11 haridirekomendasikan sebagai sumber lipase.

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