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HAYATI Journal of Biosciences
ISSN : 19783019     EISSN : 20864094     DOI : -
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 18 Documents
Search results for , issue "Vol. 27 No. 2 (2020): April 2020" : 18 Documents clear
Isolation, Selection and Identification of Polyaromatic Hydrocarbons (PAHs) Degrading Bacteria from Heavy Oil Waste (HOW)-Contaminated Soil Mohamad Yani; Charlena Charlena; Zainal Alim Mas’ud; Iswandi Anas; Yadi Setiadi; Agung Dhamar Syakti
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.935 KB) | DOI: 10.4308/hjb.27.2.142

Abstract

The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.
Isolation, Selection and Identification of Polyaromatic Hydrocarbons (PAHs) Degrading Bacteria from Heavy Oil Waste (HOW)-Contaminated Soil Yani, Mohamad; Charlena, Charlena; Mas?ud, Zainal Alim; Anas, Iswandi; Setiadi, Yadi; Syakti, Agung Dhamar
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.935 KB) | DOI: 10.4308/hjb.27.2.142

Abstract

The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.
Maggot Meal (Hermetia illucens) Substitution on Fish Meal as Source of Animal Protein to Growth, Feed Utilization Efficiency, and Survival Rate of Milkfish (Chanos chanos) Vivi Endar Herawati; Pinandoyo Pinandoyo; Seto Windarto; Putut Hariyadi; Johannes Hutabarat; YS Darmanto; Nurmanita Rismaningsih; Slamet Budi Prayitno; Ocky Karna Radjasa
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.862 KB) | DOI: 10.4308/hjb.27.2.154

Abstract

High protein content in maggot is one of the advantages for increasing fish growth through artificial feed. This study aimed to find the best optimization and feed formulation for fish meal substitution with maggot meal on growth, feed utilization efficiency, and survival rate of milkfish (C. chanos). Milkfish (C. chanos) with an average weight of 0.62±0.01 gram/fish was used as test fish. Feeding is carried out at 07.00 a.m., 12.00 p.m., and 17:00 p.m. through fixed feeding rate method. The tested fish was kept for 42 days with a stocking density of 1 fish/l. The experimental design used was a completely randomized design with five treatments and three replications. The treatments which had been done were fish meal substitution with maggot meal as follows: A (0%), B (25%), C (50%), D (75%), and E (100%). The research parameters included total feed consumption (TFC), feed utilization efficiency (FUE), protein efficiency ratio (PER), relative growth rate (RGR), survival rate (SR), and water quality. The results showed that the fish meal substitution with maggot meal had a significant effect (p<0.05) on FUE, PER, RGR and had no significant effect (p>0.05) on TFC and SR. The best treatment of each treatment is in treatment C with a composition of 50% maggot meal substitution on fish meal which resulted in a TFC value of 40.17±4.58, FUE of 27.51±0.77, PER of 0.83±0.03%, and RGR of 2.34±0.10.
Maggot Meal (Hermetia illucens) Substitution on Fish Meal as Source of Animal Protein to Growth, Feed Utilization Efficiency, and Survival Rate of Milkfish (Chanos chanos) Herawati, Vivi Endar; Pinandoyo, Pinandoyo; Windarto, Seto; Hariyadi, Putut; Hutabarat, Johannes; Darmanto, YS; Rismaningsih, Nurmanita; Prayitno, Slamet Budi; Radjasa, Ocky Karna
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.862 KB) | DOI: 10.4308/hjb.27.2.154

Abstract

High protein content in maggot is one of the advantages for increasing fish growth through artificial feed. This study aimed to find the best optimization and feed formulation for fish meal substitution with maggot meal on growth, feed utilization efficiency, and survival rate of milkfish (C. chanos). Milkfish (C. chanos) with an average weight of 0.62±0.01 gram/fish was used as test fish. Feeding is carried out at 07.00 a.m., 12.00 p.m., and 17:00 p.m. through fixed feeding rate method. The tested fish was kept for 42 days with a stocking density of 1 fish/l. The experimental design used was a completely randomized design with five treatments and three replications. The treatments which had been done were fish meal substitution with maggot meal as follows: A (0%), B (25%), C (50%), D (75%), and E (100%). The research parameters included total feed consumption (TFC), feed utilization efficiency (FUE), protein efficiency ratio (PER), relative growth rate (RGR), survival rate (SR), and water quality. The results showed that the fish meal substitution with maggot meal had a significant effect (p<0.05) on FUE, PER, RGR and had no significant effect (p>0.05) on TFC and SR. The best treatment of each treatment is in treatment C with a composition of 50% maggot meal substitution on fish meal which resulted in a TFC value of 40.17±4.58, FUE of 27.51±0.77, PER of 0.83±0.03%, and RGR of 2.34±0.10.
Selection of early maturing and high yielding mutants of Toraja Local Red Rice Grown from M2-M3 Population after Ion Beam Irradiation Rinaldi Sjahril; A. R. Trisnawaty; Muhammad Riadi; Rafiuddin Rafiuddin; Tadashi Sato; Kinya Toriyama; Yoriko Hayashi; Tomoko Abe
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.258 KB) | DOI: 10.4308/hjb.27.2.166

Abstract

This study aims to obtain the genotype of local Toraja red rice M2 mutants that have the potential to be developed into early maturing varieties and have high yields. Research carried out with a mass selection method consisting of two irradiation treatments, namely: (1) irradiation with a Carbon ion dose of 150 Gy (PL-C); (2) irradiation with Argon ion dose of 10 Gy (PL-A) and control as a comparison. Selected strains of M2 seed population were planted in experimental strains plus 2 strains as control, each row had 50 plants. Selection is done in two stages. The first stage, the selection is based on the criteria of plant growth components, to select rice plants that have early-middle age characters, dwarf-semi-dwarf, and many tillers. The second stage, selected strains from the scoring method were then grouped based on the degree of similarity with the cluster analysis method. Irradiation with ion beams produces mutants with shorter harvest times than their parents, with the harvest time 16-17 days or 10% shorter compared to their parents. This study produced 10 selected strains, 5 strains of carbon ion and 5 strains of argon ion irradiation treatment, and formed 5 groups with a 90% similarity coefficient.
Selection of early maturing and high yielding mutants of Toraja Local Red Rice Grown from M2-M3 Population after Ion Beam Irradiation Sjahril, Rinaldi; Trisnawaty, A. R.; Riadi, Muhammad; Rafiuddin, Rafiuddin; Sato, Tadashi; Toriyama, Kinya; Hayashi, Yoriko; Abe, Tomoko
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.258 KB) | DOI: 10.4308/hjb.27.2.166

Abstract

This study aims to obtain the genotype of local Toraja red rice M2 mutants that have the potential to be developed into early maturing varieties and have high yields. Research carried out with a mass selection method consisting of two irradiation treatments, namely: (1) irradiation with a Carbon ion dose of 150 Gy (PL-C); (2) irradiation with Argon ion dose of 10 Gy (PL-A) and control as a comparison. Selected strains of M2 seed population were planted in experimental strains plus 2 strains as control, each row had 50 plants. Selection is done in two stages. The first stage, the selection is based on the criteria of plant growth components, to select rice plants that have early-middle age characters, dwarf-semi-dwarf, and many tillers. The second stage, selected strains from the scoring method were then grouped based on the degree of similarity with the cluster analysis method. Irradiation with ion beams produces mutants with shorter harvest times than their parents, with the harvest time 16-17 days or 10% shorter compared to their parents. This study produced 10 selected strains, 5 strains of carbon ion and 5 strains of argon ion irradiation treatment, and formed 5 groups with a 90% similarity coefficient.
Molecular Characterization and Phylogenetic Relationships of Melon (Cucumis melo L. Meloni Cultivar) Based on Expression of Andromonoecious Gene Eka Wasi’ Al-Mughni; Budi Setiadi Daryono
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.691 KB) | DOI: 10.4308/hjb.27.2.174

Abstract

Melon (Cucumis melo L.) is one of horticultural commodities with good economic prospect for melon farmers because of several advantages. 'Meloni' is result of crossing between ‘Sun Lady-3’ and ‘PI 371795’ by Genetics and Breeding Laboratory, Faculty of Biology, UGM. The 'Meloni' plant produces females flowers that expressed by CmACS-7 gene and it can be expected that melon plants 'Meloni' included in the primitive cultivars, while 'Meloni' is a cultivation cultivar. The aim of this research is to study the relation of 'Meloni' kinship to primitive and modern cultivars. Characterization of the CmACS-7 gene is begun by a total isolation process of RNA from a sample of melon plant flowers and detection using a specific primer. The cDNA bands obtained are sequenced and then phylogenetic analysis is performed based on CmACS-7 gene expression using MEGA 7.0. Result of this study show that the presence of the CmACS-7 gene in female and hermaphrodite flowers of ‘Meloni’, ‘PI 371795’, ‘Sun Lady-3’, and ‘Hikapel’. Phylogenetic tree analyze show that ‘Meloni’ from female plant, ‘PI 371795’ and ‘Sun Lady-3’ have closely relation, while 'Hikapel' separated from other cultivars.
Molecular Characterization and Phylogenetic Relationships of Melon (Cucumis melo L. Meloni Cultivar) Based on Expression of Andromonoecious Gene Al-Mughni, Eka Wasi?; Daryono, Budi Setiadi
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.691 KB) | DOI: 10.4308/hjb.27.2.174

Abstract

Melon (Cucumis melo L.) is one of horticultural commodities with good economic prospect for melon farmers because of several advantages. 'Meloni' is result of crossing between ‘Sun Lady-3’ and ‘PI 371795’ by Genetics and Breeding Laboratory, Faculty of Biology, UGM. The 'Meloni' plant produces females flowers that expressed by CmACS-7 gene and it can be expected that melon plants 'Meloni' included in the primitive cultivars, while 'Meloni' is a cultivation cultivar. The aim of this research is to study the relation of 'Meloni' kinship to primitive and modern cultivars. Characterization of the CmACS-7 gene is begun by a total isolation process of RNA from a sample of melon plant flowers and detection using a specific primer. The cDNA bands obtained are sequenced and then phylogenetic analysis is performed based on CmACS-7 gene expression using MEGA 7.0. Result of this study show that the presence of the CmACS-7 gene in female and hermaphrodite flowers of ‘Meloni’, ‘PI 371795’, ‘Sun Lady-3’, and ‘Hikapel’. Phylogenetic tree analyze show that ‘Meloni’ from female plant, ‘PI 371795’ and ‘Sun Lady-3’ have closely relation, while 'Hikapel' separated from other cultivars.

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