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INDONESIA
MEDIA PETERNAKAN - Journal of Animal Science and Technology
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Articles 9 Documents
Search results for , issue "Vol. 32 No. 1 (2009): Media Peternakan" : 9 Documents clear
Biometri Organ Reproduksi Bagian Luar dan Karakteristik Ejakulat Anoa (Bubalus sp.) yang Dikoleksi Menggunakan Elektroejakulator Setelah Diinjeksi hCG Yudi Yudi; T L Yusuf; B Purwantara; D Sajuthi; S Mulyono; J Manansang
Media Peternakan Vol. 32 No. 1 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

This experiment aimed to investigate characteristics of reproductive organ (external parts) of the anoa (Bubalus sp.) and semen characteristics that collected by using electroejaculator after hCG injection, as an initial step on implementation of assisted reproductive techniques. Anoa is Indonesian endemic fauna that included into Appendix I by CITES. Anoa breeding efforts have not succeeded, due to their agressivity, soliter, wildness and monogamous. This experiment was carried out in Ragunan Zoo (Jakarta), Taman Safari Indonesia (Bogor), and Divison of Reproduction, Obstetrics and Gynaecology Faculty of Veterinary Medicine, Bogor Agricultural University (IPB). Biometry of reproductive organ were measured by microcaliper and nylon tape. Semen was collected by electroejaculator, under anesthetized condition. Ejaculate was evaluated macro- and microscopically. The results showed that the reproductive organ was larger as it is getting older. But, it was generally smaller compared to the buffalo's. The characteristics of fresh semen were: volume 1.02±0.28 ml, pH 6.93±0.19, mass movement 0, progressive motility 52.50±18.91%, live-death ratio 67.48±8.24%, concentration 263.33±105.06 billion/mL and total abnormality 31.86±3.72% (primary abnormality 9.52±3.85%, secondary abnormality 22.33±1.46%). The semen qualities were fairly good, even though some parameters showed lower number compared to buffalo's. It is important to improve the collection technique, animal handling and appropriate extender development for semen cryopreservation. Key words: anoa, biometry of external reproductive organ, semen characteristics, electroejaculator
Kualitas Spermatozoa Tikus yang Diberi Tepung Kedelai Kaya Isoflavon, Seng (Zn) dan Vitamin E S Astuti; D Muchtadi; M Astawan; B Purwantara
Media Peternakan Vol. 32 No. 1 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The objective of this experiment was to evaluate the effects of isoflavone-riched soybean flour, zinc (Zn) and vitamin E on quality of rats spermatozoa as animal model. Diet was given as isonitrogen and isocaloric with 15% of dietary protein. Thirty male Sprague Dawley post weaning rats (21 days old) were divided into six groups and treated with isoflavone-riched soybean flour, Zn and vitamin E in different combination. Isoflavone-riched soybean flour (3 mg/day) was given by oral administration, whereas Zn and vitamin E were mixed with the basic diet. The treatments were given for 2 months. Thirty female Sprague Dawley post weaning rats (21 days old) were used to observe male fertility. Results indicated that synergic interaction between isoflavone-riched soybean flour, Zn and vitamin E increased motility and concentration of rats spermatozoa. The treatment was not significant on relative weight of testis, conception rate and foetus size. The best results was shown by the group which was given isoflavone-riched soybean flour with diet containing both Zn and vitamin E, i.e. motility rate of 79.50±1.12%, sperm concentration of 1636.90±87.95 million/ml, and abnormality rate of 9.00±0.70%. Conception rate and the number of foetus on female rats were 100% and 11±0.71, respectively. It is concluded that combination treatment of isoflavone-riched soybean flour, Zn and vitamin E on male rats showed the best fertility in comparison with single treatment and the other combination. Key words: isoflavone-riched soybean, Zn, vitamin E, quality of spermatozoa, rat
Sifat Listrik Telur Ayam Kampung Selama Penyimpanan J Juansah; Irmansyah Irmansyah; Kusnadi Kusnadi
Media Peternakan Vol. 32 No. 1 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Study on eggs properties, based on electrical measurement (conductance and capacitance) was carried out using alternating current signal on frequency variation from 10 Hz to 100 kHz. The electrical properties correlated to physical properties of eggs i.e haugh unit, pH and viscosity. The albumen and yolk of native eggs, which were placed in the cell system from acrylic and electrode plate conductor of cuprum, were used as samples. Hi LCR Tester Hioki with dual probe (modified from four probes) was used to measure electrical properties of samples. This equipment was provided with fixed current source but varies in frequency. The conductance of yolk and albumen increased exponentially if frequency signal was boosted up. The electrical conductance range of albumen was higher than yolk on all frequencies. The capacitance property decreased if frequency signal was boosted up. The decrease was exponential especially at frequency under 100Hz. The albumen capacitance was lower than yolk. The viscosity of yolk and albumen during storage was decreasing. The value of pH was not affected. The pH of albumen was between 9 and 10, and yolk was about 6. Multiple regression method was used to get empiric equation of electrical conductance, capacitance, HU and viscosity. The best correlation between electrical parameter and physical properties is conductance equation of HU and viscosity at 1 kHz. Key words: electrical properties, conductance, capacitance, native chicken, egg
Analisis D-loop DNA Mitokondria untuk Memposisikan Ayam Hutan Merah dalam Domestikasi Ayam di Indonesia S Sulandari; M.S A Zein
Media Peternakan Vol. 32 No. 1 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The current poultry is a domesticated chickens used for both meat and egg production. Pedigree investigation is an important part to understand the process of chicken domestication in Indonesia. Molecular DNA approach using D-loop Mitochondrial DNA marker (hypervariable 1 segment) was used in this analysis. The objective of the study was to construct the pedigree analysis of Indonesian chicken. Four hundreds and eighty four (434) samples belonging to 15 breeds of Indonesian local chicken (Cemani, Kedu, Kedu Putih, Pelung, Sentul, Wareng, Merawang, Kapas, Kate, Arab Silver, Arab Gold, Gaok, Nunukan, Kalosi and Tolaki) and 9 samples of Red jungle fowls (Gallus gallus gallus) were extracted, PCR amplified and subsequently sequenced. Four sequence references from GeneBank, Gallus gallus (NCBI, accession number AB0986688). G. gallus (GenBank accession number AB098668), G. gallus spadiceus (GenBank accession number AB007721), and G. gallus bankiva (GenBank accession number AB007718) were included in this analysis. The sequences of the first 397 nucleotides were used for analysis. The results show that 72 haplotypes were identified from 56 polymorphic sites. Phylogenetic analysis showed that Indonesian chicken have a close relationship with 2 subspecies of Gallus gallus (G. gallus gallus and G. gallus spadiceus). Our results suggest that D-loop region is highly variable in Indonesian chicken with large number of haplotypes. Key words: Gallus gallus, Indonesian chicken, D-loop Mitochondrial DNA
Pertumbuhan Optimum Penicillium spp. dan Cunninghamella spp. yang Diisolasi dari Pakan dan Efek Toksiknya pada Mencit (Mus musculus) N Ramli; M Tafsin; A D Hasjmy
Media Peternakan Vol. 32 No. 1 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Fungi are a major microorganism present in the feedstuffs that able to reduce nutritive value and produce toxin that harmful for animal. Penicillium spp. and Cunninghamella spp. were dominant present in the feedstuffs in tropical regions. The objectives of this study were to find out the optimum pH and temperature of Penicillium spp. and Cunninghamella spp. on agar media and to find out the effect of feeding diet containing corn contaminated with the fungi at level of 0%, 50% and 100% on the performance and relative organ weight of 45 mice (Mus musculus). Cunninghamella spp. grew at pH range of 3-9, and Penicillium spp. at pH range of 3-7. The temperature for the optimum growth of both fungi was on room temperature (28±2 oC). Feed consumption and daily gain (ADG) of mice were not significantly affected by content of corn contaminated with Cunninghamella spp. Conversely, corn contaminated with Penicillium spp. significantly (P < 0.05) reduced feed consumption and ADG of mice. Treatments had no effect on liver and hearth relative weight, but significantly influenced relative weight of kidney and lymph. Lymph relative weight of mice fed ration containing Penicillium-contaminated corn was lower (P < 0.05) than that of control. Mice treated with contaminated corn from both fungi at the level 100% also significantly (P < 0.05) had higher kidney relative weight than that of control. It was concluded that the toxic effect of Penicillium spp. was higher than that of Cunninghamella spp. Key words: fungi, feedstuffs, growth, toxin, mice
Substitusi Rumput Lapangan dengan Kulit Buah Coklat Amoniasi dalam Ransum Domba Lokal M Zain
Media Peternakan Vol. 32 No. 1 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

An experiment was aimed to evaluate the effect of substitution of native grass with ammoniated cocoa pod on digestibility and live weight gain of sheep. Three treatments were arranged in a randomized block design consisting of five replicates. The total of 15 head of 8-14 months old lokal sheeps with initial live weight 9-12 kg were used. The treatments were A= 50% native grass and 50% concentrate, B= 25% native grass+ 25% ammoniated cocoa pod + 50% concentrate C= 50% ammoniated cocoa pod + 50% concentrate. The results indicated that substitution of native grass with ammoniated cocoa pod did not significantly influence feed intake, digestibility, live weight gain and diet efficiency. It was indicated that ammoniated cocoa pod can be used to replace the native grass in sheep diet. Key words: cocoa pod, ammoniated, sheep, gain
Ruminal Methane Production on Simple Phenolic Acids Addition in in Vitro Gas Production Method A Jayanegara
Media Peternakan Vol. 32 No. 1 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Methane production from ruminants contributes to total global methane production, which is an important contributor to global warming. In this experiment, six sources of simple phenolic acids (benzoic, cinnamic, phenylacetic, caffeic, p-coumaric and ferulic acids) at two different levels (2 and 5 mM) added to hay diet were evaluated for their potential to reduce enteric methane production using in vitro Hohenheim gas production method. The measured variables were gas production, methane, organic matter digestibility (OMD), and short chain fatty acids (SCFA). The results showed that addition of cinnamic, caffeic, p-coumaric and ferulic acids at 5 mM significantly (P p-coumaric > ferulic > cinnamic. The addition of simple phenols did not significantly decrease OMD. Addition of simple phenols tends to decrease total SCFA production. It was concluded that methane decrease by addition of phenolic acids was relatively small, and the effect of phenolic acids on methane decrease depended on the source and concentration applied. Key words: methane, phenolic acids, in vitro, rumen
Performa dan Profil Beberapa Komponen Darah Sapi Peranakan Ongole yang Diberi Pakan Mengandung Lerak (Sapindus rarak De Candole) D A Astuti; E Wina; B Haryanto; S Suharti
Media Peternakan Vol. 32 No. 1 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The problem with low meat production is caused by several factors, ie: management, feed and animal health. The development of integration system such as cattle-rice, cattle-corn and cattle-palm oil aims to utilize feed from local resources. However, sometimes local feedstuff causes a problem of imbalance nutrient. There are several strategies to overcome this problem and one of them is the use of feed additives from natural substance or plant resources such as lerak (Sapindus rarak De Candole). This research was aimed to utilize lerak fruit to improve performance and evaluate some blood profiles of Ongole crossbred cattle. The in vivo work was done using 12 Ongole crossbred cattle receiving 3 different feeds as treatment, i.e. 1) feed without lerak (high roughage diet) as control, 2) feed contained 2,5% lerak, and 3) feed contained 5% lerak. Growth, daily gain, blood profiles (leucocytes, cholesterol, trigliceride and globulins) were observed. The results showed no significant differences for nutrient intake, meaning that the ration has good palatability. Average daily gain of cattle fed with 2,5% lerak was 20% higher than those with control diet. White blood cells tended to decrease caused by lerak treatments, but the globulin profiles (alpha, beta and gamma) were not significantly affected in all treatments. Blood cholesterol concentration decreased following the increase of lerak percentage. It is not recommended to use lerak in the cattle ration for more than two months because of the decreasing of leucocytes. Key words: Sapindus rarak, in vivo, leucocytes, globulins, palatability
Pola Pertumbuhan Rumput Signal (Brachiaria humidicola (Rendle) Schweick) pada Padang Penggembalaan dengan Aplikasi Sumber Nutrien Berbeda L Abdullah
Media Peternakan Vol. 32 No. 1 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

An investigation on growth behavior parameters and its dynamic pattern of signal grass (Brachiaria humidicola) grown under influence of inorganic and organic nutrient supply was conducted during period of wet and early dry seasons in 2004. Five sets of treatments consisted of control (P0), mulch originated from pasture weed biomass (Chromolaena odorata) (PC), animal dung (PF), combination of mulch and dung (PC+F) and inorganic fertilizer (PA) were applied to one year existing signalgrass plots. Block randomized design with 4 replications was used in this experiment. The results showed that application of PA produced the highest length of stolons, node and tiller numbers. There was an improvement of growth by application of PC, PF and PC+F, but at lower level than those of PA. Application of PA caused short growth period (6-8 weeks) to reach maximum length of stolon, node and tiller numbers, but less persistent (10-12 weeks) if the grass had not been defoliated. On the other hand, application of organic nutrient led to slow growing grass, but it showed more persistent. The application of organic nutrient supply (PC, PF and PC+F) resulted the best growth of signal grass in comparison with control and more persistent than that of inorganic fertilizer (PA). Key words: Brachiaria humidicola, stolon, tiller, node, mulch

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