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INDONESIA
MEDIA PETERNAKAN - Journal of Animal Science and Technology
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Core Subject : Agriculture,
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Articles 12 Documents
Search results for , issue "Vol. 34 No. 1 (2011): Media Peternakan" : 12 Documents clear
Association of Polymorphisms Calpastatin Gene with Body Weight of Local Sheep in Jonggol, Indonesia Sutikno Sutikno; M Yamin; C Sumantri
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (795.408 KB) | DOI: 10.5398/medpet.2011.34.1.1

Abstract

Calpastatin (CAST) gene is located on the fifth chromosome of sheep and plays important roles in formation of muscles and meat tenderness after slaughtering. Association of genetic polymorphism in the CAST gene locus MspI and NcoI with body weight was examined in local sheep from Jonggol Animal Science Teaching and Research Unit (JASTRU), Faculty of Animal Science, Bogor Agricultural University. The genotypes for CAST were determined by the PCR-RLFP method. Blood samples were collected from 264 local sheep belonging to JASTRU located in Singosari Village, Bogor District, West Java Province. Extraction of genomic DNA was based on the phenol chloroform method. CAST locus MspI had three genotypes including in MM, MN and NN with frequencies of 0.75, 0.23, and 0.02 respectively. CAST locus NcoI had two genotypes including in MM and MN with frequencies of 0.92, 0.08 respectively. Chi-square test confirmed Hardy-Weinberg equilibrium for the CAST locus MspI and NcoI. There was no significant effects (P>0.05) of CAST locus MspI and NcoI genotypes on body weight of local sheep in JASTRU.
Gambaran Hematologi Tikus Putih (Rattus norvegicus) yang Diinfeksi Escherichia coli Enteropatogenik dan Diberikan Probiotik M Astawan; T Wresdiyati; I I Arief; E Suhesti
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.513 KB) | DOI: 10.5398/medpet.2011.34.1.7

Abstract

In Indonesia, diarrhea is the second leading cause of death in children after ISPA (Acute Respiratory Infection). This study aimed to test the ability of probiotic, Lactobacillus plantarum 2C12 and Lactobacillus acidophilus 2B4, as an indigenous anti-diarrhea in rats exposed to Enteropathogenic Escherichia coli (EPEC) and find out their impact on erythrocytes, hematocrit, hemoglobin, thrombocytes, and leukocytes. A total of 95 male rats Sparague Dawley strain were alocated into six treatment groups: (1) negative control, (2) L. plantarum 2C12, (3) L. acidophilus 2B4, (4) L. plantarum 2C12 + EPEC, (5) L. acidophilus 2B4 + EPEC, and (6) positive control (exposed to EPEC). Probiotics were given from day 1 up to 21, whereas 106 cfu/ml EPEC was administered orally from day 8 to 14. On day 21, the number of erythrocytes, hematocrit and hemoglobin were the lowest (7.07 million/µl; 39.13%; 14.50 g/dl) in the positive group of rats. The positive control group had lower number of thrombocytes (389 thousand/ µl) (P < 0.05) with the negative control and the L. plantarum 2C12 groups, but not significantly different to the other groups. The number of leucocytes in the positive control rats (4433 cell/µl) was lower (P < 0.05) than that of the negative control, L. plantarum 2C12, and the L. acidophilus 2B4 groups, but no significant difference to the other groups. L. plantarum 2C12 had better ability than L. acidophilus 2B4 in maintaining the number of erythrocytes, hematocrit, and hemoglobin of rat infected by EPEC.
PCR-RFLP Using BseDI Enzyme for Pork Authentication in Sausage and Nugget Products Y Erwanto; M Z Abidin; A Rohman; . Sismindari
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3791.907 KB) | DOI: 10.5398/medpet.2011.34.1.14

Abstract

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using BseDI restriction enzyme had been applied for  identifying the presence of pork in processed meat (beef sausage and chicken nugget) including before and after frying. Pork sample in various levels (1%, 3%, 5%, 10%, and 25 %) was prepared in a mixture with beef and chicken meats and processed for sausage and nugget. The primers CYTb1 and CYTb2 were designed in the mitochondrial cytochrome b (cyt b) gene and PCR successfully amplified fragments of 359 bp. To distinguish existence of porcine species, the amplified PCR products of mitochondrial DNA were cut by BseDI restriction enzyme. The result showed pig mitochondrial DNA was cut into 131 and 228 bp fragments. The PCR-RFLP species identification assay yielded excellent results for identification of porcine species. It is a potentially reliable technique for pork detection in animal food processed products for Halal authentication.
Muscle Growth and Distribution in Fattening Steer of Different Breeds R Priyanto; E R Johnson
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.824 KB) | DOI: 10.5398/medpet.2011.34.1.19

Abstract

The breed patterns in growth and distribution of muscle were studied using three breeds of beef cattle entering fattening phase. This study involved 23 grass-fed steer Brahman, 24 Hereford and 22 Brahman x Hereford crosses with a live weight range of 300 to 600 kgs. An allometric Huxley model was used to study the growth and distribution patterns of muscle tissue within wholesale cut.  There were several cuts in which the muscle growth coefficients were significantly different among breeds. Comparisons of muscle weight distribution at log natural of 75 kg side muscle + bone weight (4.313 kg) generally showed significant between-breed differences. Herefords tended to have more muscles in their wholesale cuts than Brahmans and/or Brahman x Hereford crosses, except the muscles in Tenderloin, Rib Set and Chuck. Brahmans had heavier Topside and Silverside than Herefords and Brahman x Hereford crosses and the differences were maintained at log natural of 114 kg side muscle + bone weight (4.733 kg).  At this heavier weight, there was a general tendency for Brahmans to have more muscle in their wholesale cuts than the Brahman x Hereford steers, except the muscle in Loin and Neck + Sticking.
Characterization of Partial Coding Region Fibroin Gene on Wild Silkmoth Cricula trifenestrata Helfer (Lepidoptera: Saturniidae) Suriana Suriana; D D Solihin; R R Noor; A M Thohari
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.84 KB) | DOI: 10.5398/medpet.2011.34.1.23

Abstract

The study was conducted to characterize coding region of wild silkmoth C. trifenestrata partial fibroin gene, and detect these gene potential as molecular marker. A total of six larvae C. trifenestrata were collected from Bogor, Purwakarta and Bantul Regency. Genomic DNA was extracted from silk gland individual larvae, then amplified by PCR method and sequenced. DNA sequenced result was 986 nucleotide partial fibroin gene of C. trifenestrata, which are comprising complete coding region of first exon (42 nucleotide), an intron (113 nucleotide), and partial of second was exon (831 nucleotide). Only coding region was characterized. Results showed that first exon very conserved in C. trifenestrata. These gene consisted of 31%, thymine, 28% guanine, 21% cytosine, and 19% adenine. Cytosine and thymine (sites of 25th and 35th respectively) were marker for C. trifenestrata species. The first exon encoding 14 amino acids. Valine amino acid (12th site) was marker to the species C. trifenestrata. The partial second exon consisted of guanine (32.7%), alanine (26.5%), thymine (21%) and cytosine (19.7%). These region encoded 277 amino acids, which were dominated by the alanine (27.8%) and glycine (21.66%). Alanine formed polyalanine sequence with different motifs namely: AAAAAAASS, AAAAAAAAAAAGSSG, AAAAAAAAAAAAGSGTGFGGYDS, AAAAAAAAAAGSSGRGGYDGVDGGYGSGSS, and AAAAAAAAAAAAGSSGRGLGGYDGWVDDGYGSGSGS.
Jenis dan Kandungan Tanin Pakan Satwa Anoa (Bubalus sp.) M Basri; Rukmi Rukmi
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1806.427 KB) | DOI: 10.5398/medpet.2011.34.1.30

Abstract

Study of kinds and tannin content of diets consumed by anoa is limited. This information is very important and necessary for anoa domestication. The aims of this research were to obtain information on kinds and tannin content of anoa diets. The research was conducted in forest of Pangi Binangga nature reserve and Lore Lindu National Park, province of Central Sulawesi in March to November 2009. Digestive content analyses method was used to study the kinds of diets consumed by anoa. Chromatography was used for tannin analysis. Results of the digestive content analysis found ten kinds of anoa diets, i.e. Ficus miquelly, Caryota mitis, Ficus ampelas, Syzigium sp., Cordia mixab, Paspalum conjugatum, Smilax leocophylla, Saccarum spontaneum, Isachne globosa and Imperata cylindrica. The proportion of the diets were 60%, 45%, 35%, 17%, 12%, 10%, 8%, 8%, 5%, and 4%, respectively. Three of six diets namely Ficus miquelly, Saccarum spontaneum and Isachne globosa contained low tannin. The tannin content of three diets was 0.34%, 0.61%, and 0.99%, respectively. It is concluded that anoa in forest prefer diets that contain low tannin, such as Ficus fruits, shoots of shrubs.
Performa Kambing yang Diberi Kulit Buah Kakao Terfermentasi Suparjo Suparjo; K G Wiryawan; E B Laconi; D Mangunwidjaja
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.846 KB) | DOI: 10.5398/medpet.2011.34.1.35

Abstract

Utilization of cocoa pod husk (CPH) as feedstuff needs pretreatment to increase its nutrients availability. Bioconversion with Phanerochaete chrysosporium changes its structure by breaking down the linkage between lignin and structural carbohydrates. This experiment was aimed to evaluate the quality of fermented CPH biomass as feed for goats. The experimental treatments i.e.:  A= 30% of fresh napier grass (RG) + 50% of dried RG + 20% of concentrate; B= 30% of fresh RG + 30% of dried RG + 40% of concentrate; C= 30% of fresh RG + 30% of CPH + 40% of concentrate; D= 30% of fresh RG + 30% of fermented CPH + 40% of concentrate and E= 30% of fresh RG + 50%  of fermented CPH + 20% of concentrate. The treatments were allocated in a randomized block design with three replications.  Feed intake, body weight gain and ration efficiency were measured. The use of fermented CPH at the level of 30% had higher (P < 0.05) feed intake (560.33 g day-1), body weight gain (101.79 g head-1 day-1), and feed conversion (5.50) compared to other treatments. In conclusion that the use of 30% fermented CPH in the ration showed the best body weight gain and feed efficiency.
Ketahanan Asam Lemak Tidak Jenuh dalam Crude Palm Oil Terproteksi terhadap Aktivitas Mikrob Rumen Domba in Vitro N C Tiven; L M Yusiati; Rusman Rusman; U Santoso
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.885 KB) | DOI: 10.5398/medpet.2011.34.1.42

Abstract

The experiment was aimed to study the composition of unsaturated fatty acid, fermentation parameter and microbial activity in the rumen-buffer mixture when protected CPO with casein and formaldehyde was incubated in the in vitro fermentation. CPO and milk powder (1:2) were mixed, added technical formaldehyde 0%, 1%, 2%, and 3% by weight of blend, mixed again to form CCPO. The amount of 0% (0 mg), 1.5% (4.5 mg), 3% (9 mg), 4.5% (13.5 mg) and 6% (18 mg) CCPO was inserted into fermenter bottles containing napier grass and rice bran (60:40), was tested in vitro. The data were analyzed by completely randomized design with factorial pattern 4 x 5 (level of formaldehyde as factor A and level of CPO as factor B). The different of treatments were tested by Duncan's new multiple range test.  Result shows that the increase of formaldehyde level could increase unsaturated fatty acids content, CMC-ase activity, microbial protein and number of protozoa, but decreased NH3, VFA, and pH of rumen fluid. The increase of CPO level also increased fatty acid composition and microbial protein, but decreased NH3, VFA, and pH, CMC-ase activity and number of protozoa in rumen fluid. It can be concluded that encapsulated CPO with formaldehyde increased unsaturated fatty acid composition and microbial activity, but decreased fermentation parameters.
Pertumbuhan Kompensasi dan Efisiensi Produksi Ayam Broiler yang Mendapat Pembatasan Waktu Makan A Azis; H Abbas; Y Heryandi; E Kusnadi
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.179 KB) | DOI: 10.5398/medpet.2011.34.1.50

Abstract

The present study was designed to investigate the effect of feeding time restriction on compensatory growth and production efficiency of broiler chickens. Four hundred of 7 days-old unsexed broiler chicken of Lohmann commercial strain were used in the experiment. The treatments were: bird fed ad libitum as a control treatment (R-0); bird had free access to feed during two periods of 2 hours (08:00-10:00 and 16:00-18:00) from 7-14 days of age (R-1) and 7-21 days of age (R-2); bird had free access to feed during two periods of 4 hours (08:00-12:00 and 16:00-20:00) from 7-14 days of age (R-3) and 7-21 days of age (R-4). The treatments were arranged in a completely randomized design with 4 replications. Feed intake and body weight gain of chicken exposed to feeding time restriction on R-1, R-2, R-3 and R-4 were lower (P < 0.01) than those of chicken fed ad libitum. There were no significant different on feed intake and body weight gain during realimentation period. Compensatory growth occured on R-2 and R-4 during the 28 to 42 days of age and similar body weight  with control at age 42 days. Feed conversion ratio on R-2 and R-4 were lower (P < 0.01) feeding time restriction (7-21 days) and realimentation period (28-35 days). It was concluded that feeding time restriction for 8 hours per day from 7 to 21 days of age indicated compensatory growth and similar body weight and had better feed cost and production index than that of control.
Laying Performance of Wareng Chicken under Free Choice Feeding and Different Cage Density S Iskandar
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.51 KB) | DOI: 10.5398/medpet.2011.34.1.58

Abstract

The aim of the experiment was to explore the possibility of using free choice feeding technique under different cage density for unselected native chicken. Eighty hens of laying Wareng chicken were divided into two feeding systems. Half of them were fed complete commercial diet of 17% crude protein, with 2800 kcal ME/kg and the other half were subjected to free choice diet. The diets were in mash form and placed in sufficient feed troughs in front of each cage. Each cage was occupied by four or six hens. Free choice feeding hens were served with commercial concentrate (30% crude protein), ground corn and oyster shell, placed separately in feed troughs. Observation was carried out during 24 weeks laying period, starting from 20 to 44 weeks of age. The results showed that egg production (9.35% hen day), and the intake of feed (42.74 g/day), protein (7.01 g/day), energy (116.6 kcal ME/day), calcium (1.99 g/day and phosphorus (0.22 g/day) were not affected (P>0.05) by feeding system nor by cage density. The results however indicated that free choice feeding technique provided sufficient nutrients in supporting maximum egg production of unselected native chicken.

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