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Jurnal Fitopatologi Indonesia
ISSN : 02157950     EISSN : 23392479     DOI : -
Core Subject : Health,
Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).
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Articles 5 Documents
Search results for , issue "Vol 16 No 2 (2020)" : 5 Documents clear
Trichoderma Asal Akar Kopi Dari Alor: Karakterisasi Morfologi dan Keefektifannya Menghambat Colletotrichum Penyebab Penyakit Antraknosa secara in Vitro Didiana Yanuarita Molebila; Ade Rosmana; Untung Surapaty Tresnaputra
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.61-68

Abstract

Trichoderma of Coffee Roots From Alor: Morphological characteristic and in vitro Efficacy to Inhibit Colletotrichum Causing Anthracnose Trichoderma is a fungus capable of intimate associations with plant root systems including on coffee plants. This aim of study is to determine the characteristics of Trichoderma morphospecies from coffee roots of Alor origin, East Nusa Tenggara (NTT) and its ability to inhibit the growth of Colletotrichum causing anthracnose disease in-vitro. Root samples of healthy coffee plants were taken from the location of coffee plantations in Alor District, NTT. Isolation of Trichoderma fungi from coffee roots was done by incubating the sterilized coffee roots in a layer of moist filter paper in a Petri dish for seven days. Identification of Trichoderma by observing the characteristics of the colony on the medium of potato dextrose agar (PDA) and microscopic media using microcultures (slide culture). Inhibition of Trichoderma fungi against Colletotrichum was tested by multiple culture methods on PDA media. The results of root incubation in humid conditions showed that there was four morphospecies of Trichoderma fungi, each of which had different characteristic specifications. In vitro antagonism in test on PDA medium, the first three morphospecies against Colletotrichum showed that each Trichoderma could inhibit 70.2%, 65.8%, and 63.3%, respectively, five days after inoculation. This data shows that Trichoderma isolated from coffee roots from Alor has the potential to suppress the growth of anthracnose pathogens.
Uromyces acori (Uredinales) Penyebab Nekrosis pada Daun Tanaman Jeringau (Acorus calamus) di Indonesia Dono Wahyuno; Marlina Puspita Sari; Dini Florina
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.81-86

Abstract

Uromyces acori (Uredinales) A Causal Agent of Rust Disease of Sweet Flag (Acorus calamus) in Indonesia In Indonesia, an asexual state of a rust fungus namely Uredo acori has been considered as the causal agent rust disease of the sweet flag. No report according sexual state is available in Indonesia. The objectives of the present study are confirming the fungal identity morphologically and determine its optimal germinating temperature. An artificial inoculation was conducted by dusting the urediniospore onto detached leaves of sweet flag. Only leaves showed uredinia were selected. The tip bases of the leaves were dipped into a 4% sucrose solution, incubated in a room condition at 25 °C for inducing telial state with teliospore formations. Based on morphological characters of the teliospore and urediniospore, the rust fungus of sweet flag was identified as Uromyces acori. The fungus is germinating optimally at 25 °C.
Pengamatan dan Pemetaan Penyakit Busuk Pangkal Batang di Perkebunan Kelapa Sawit Menggunakan Unmanned Aerial Vehicle (UAV) dan Kamera Multispektral Heri Santoso
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.69-80

Abstract

Surveillance and Mapping of Basal Stem Rot Disease in Oil Palm Plantation Using Unmanned Aerial Vehicle (UAV) and Multispectral Camera Basal stem rot (BSR) disease caused by Ganoderma boninensis is still a major disease in oil palm plantations both in Indonesia and Malaysia. In some countries, remote sensing approach has been used for monitoring BSR in oil palm plantation. However, the utilization of satellite imagery in remote sensing especially in vegetation study on the tropical region was often limited by cloud cover. A drone or unmanned aerial vehicle (UAV) utilization is the best way to deal with cloud cover in the tropic region. Machine learning of random forest (RF) and satellite imagery used in the BSR study produced good accuracy. This research was aimed to identify and monitor the BSR infection on individual oil palm trees using an UAV and multispectral camera and RF classification. The results showed that the data acquired from UAV was affected by cloud shadows. The RF classification of healthy and infected oil palm trees by BSR disease and the spreading map of BSR infection was affected by cloud shadows. The highest accuracy of healthy and infected oil palm by BSR was 79.49%. Reflectance calibrator, digital to reflectance conversion, and model implications to build spreading map of BSR infection need to be conducted both on the clear area and the cloud shadow-covered area. Moreover, the UAV-based data should be considering the cloud view on the coverage area.
Konjugat Poliklonal Antibodi Nanopartikel Emas untuk Deteksi Potato Virus Y Ifa Manzila; Tri Puji Priyatno; Fikri Hidayatullah
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.87-93

Abstract

Polyclonal Antibodi-Gold Nanoparticles for Potato Virus Y Detection Gold nanoparticles are stable colloidal solutions with dimensions of 1-100 nm having surface plasmon resonance with six free electrons. The existing of six free electrons on the surface of a plasmon causes gold nanoparticles to bind easily to various types of bioreseptors including polyclonal antibodies. Polyclonal potato virus Y (PVP) antibodi been successfully conjugate with gold nanoparticles in order to develop a rapid detection for PVY infection in potato plants. The gold nanoparticles was synthetized by the reduction of gold (III) chloride trihydrate (HAuCl4) with 1% sodium citrate. Subsequently, the nanoparticles were used to make gold nanoparticle-antiobody PVY-conjugate. PVY detection was carried out with dot blot method on the nitrocellulose membrane. The results showed that the PVY virus on the membrane can be detected 10-30 minutes after incubation, depend on the concentration of the conjugate and the concentration of the virus in the sampel. The use of gold nanoparticle conjugates can increase the efficiency of the immunodot blot method in about 1 hour, and this method can be developed to be a lateral flow system for field detection of PVY.
Komponen Epidemi Penyakit Busuk Akar dan Pangkal Batang Tebu di Sumatera Selatan Tri Maryono; Ani Widiastuti; Rudi Hari Murti; Achmadi Priyatmojo
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.49-60

Abstract

Epidemic Components of Sugarcane Root and Basal Stem Rot In South Sumatra Root and basal stem rot disease is one of new disease that currently was became a emerging problem on sugarcane plantations in Lampung and South Sumatra. The research was aimed to study the model of disease progression, AUDPC, infection rate (r), and the influence of weather and soil condition (physical and chemical properties of soil) on the development of sugarcane root and basal stem rot disease. The research was conducted on sugar cane plantation in South Sumatra. The disease incidence in three varieties was observed weekly in the field which had infected plant in previous year.. The data were analyzed to develop model of the disease progression, AUDPC, and the rate of root and basal stem rot disease. Weekly weather data was acquired from weather station of Sultan Mahmud Badaruddin Airport, Palembang. Soil sample was taken from three disease category i.e moderate (disease incidence 25,1% - 50%), severe (disease incidence 50,1% - 75%), and healthy (no disease). The results showed that the disease develops following monomolecular and logistic models. The AUPDC and infection rate (r) in the monomolecular model were lower than in the logistic model. The direct effect coefficient of rainfall and relative humidity (RH) on the disease were positive (1.27 and 0.46 respectively), contrary the temperature and duration of irradiation which had a negative coefficient (-0.33 and -0.45 respectively). Meanwhile, K availability and permeability give a positive effect on the disease, while the total N and Fe availability give a negative effect on the disease.

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