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Contact Name
Ummy Mardiana Ramdan
Contact Email
joimedlabs.aiptlmi@gmail.com
Phone
+6281223433385
Journal Mail Official
joimedlabs.aiptlmi@gmail.com
Editorial Address
Jl. Arteri JORR Jatiwarna Pondok Melati, Bekasi, Jawa Barat, Indonesia Kode Pos: 17415
Location
Unknown,
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INDONESIA
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
ISSN : 27750108     EISSN : 27742504     DOI : -
Core Subject : Health, Science,
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) merupakan jurnal AIPTLMI (Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia) yang dapat diakses secara terbuka (open acces) yang berfokus pada pengembangan ilmu pengetahuan Teknologi Laboratorium Medik (TLM) dengan tujuan untuk memperluas pengetahuan, pemikiran dan penelitian di bidang laboratorium kesehatan. Topik yang diangkat meliputi hematologi, kimia klinik, imunoserologi, imunohematologi, sitohitoteknologi, mikrobiologi, parasitologi, dan toksikologi klinik.
Articles 2 Documents
Search results for , issue "Vol 7 No 1: April 2026" : 2 Documents clear
Utilization of neem leaf extract (Azadirachta indica A. Juss.) in inhibiting the growth of acne-causing bacteria Cutibacterium acnes Sinatra, Dhimas Pramayoga; Rini, Chylen Setiyo
Journal of Indonesian Medical Laboratory and Science Vol 7 No 1: April 2026
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v7i1.284

Abstract

Background: Acne is an infection that occurs on the surface of the skin in the form of inflammation of the oil glands in the epidermal layer of the skin, when infected by the bacteria Cutibacterium acnes, blackheads will develop into inflammation and cause acne vulgaris. One of the plants that can be used as a natural alternative in acne treatment is neem (Azadirachta indica A. Juss). Objectives: This research aims to identify the inhibitory effect of neem leaf extract on C. acne. Materials and Methods: Neem leaves samples were obtained from the Sumenep district. The research was conducted at the PIPOT-UBAYA Laboratory, the Pharmacy Laboratory of PGRI Adi Buana University Surabaya and the Microbiology Laboratory of Stikes Ngudia Husada Madura. The research design used is experimental, testing of the inhibition power of neem leaves using the diffusion method by observing the inhibition zone around the disc. This research used concentrations of 25, 50, 75, and 100%. The positive and negative controls used are sterile aquades and Clindamycin. Results: The results showed the inhibition zones of neem leaf extract against C. acnes at concentrations of 25% and 50% were 6.3 mm and 9.3 mm, while at concentrations of 75% and 100% were 12.3 mm and 16 mm. Conclusions: The results of the one-way ANOVA test showed p-value is 0.000, which means that neem leaves can inhibit the growth of C. acnes.
Parijoto (Medinilla speciosa) extract as a natural dye alternative for peripheral blood smears: A comparative study with giemsa stain Trisna, Meida; Indanah, Indanah; Rusidah, Yunita; Saputro, Arief Adi; Sholikhati, Anisa; Ekawati, Sherly Nur
Journal of Indonesian Medical Laboratory and Science Vol 7 No 1: April 2026
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Peripheral blood smear staining is an essential hematological procedure used to evaluate blood cell morphology. The commonly used standard stain is Giemsa stain; however, its chemical components may pose potential risks to health and the environment. Therefore, safer natural alternatives are needed. Previous studies have reported that anthocyanin-containing plant extracts, such as Hibiscus sabdariffa, Tectona grandis, and Garcinia mangostana, can effectively stain blood cells. Medinilla speciosa (parijoto) also contains anthocyanins and has potential as a natural dye. Objective: This study aimed to evaluate the staining quality of peripheral blood smears using Medinilla speciosa extract at different concentrations and to compare the results with a 10% Giemsa stain control. Methods: This laboratory-based experimental study used a post-test only control group design. A total of 30 blood smears were stained using Medinilla speciosa extract at concentrations of 25%, 50%, and 70%, along with a 10% Giemsa stain control. The extract was prepared using maceration with pH adjustment to optimize anthocyanin stability. Staining quality was assessed based on color intensity, background clarity, and erythrocyte morphology using a semi-quantitative scoring system. Data were analyzed using the Kruskal–Wallis test and Mann–Whitney U test. Results: Increasing extract concentration was generally associated with improved staining quality. A significant difference among groups was observed (p = 0.002). Pairwise analysis showed that the 70% extract was not significantly different from the 10% Giemsa stain control (p = 0.321), while lower concentrations differed significantly. Conclusion: The 70% Medinilla speciosa extract showed the best performance among extract groups and demonstrated comparable staining quality in certain parameters. However, Giemsa stain remains the more consistent standard. Further optimization is required before routine application.

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