Berkala Penelitian Hayati
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
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<![CDATA[SEBARAN TUMBUHAN BAWAH PADA TUMBUHAN Acacia nilotica (L) Willd. ex Del. DI SAVANA BEKOL TAMAN NASIONAL BALURAN ]]>
<![CDATA[Suhadi Suhadi]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/244
<![CDATA[Bekol savannah is places of reproduction for banteng (Bos javanicus d` Alton), water buffalo (Bubalus bubalis L), large deer (Cervus timorensis) and green peafowl (Pavo mutiacus) and for conservation of species, habitat and genetic. In sector recreation and tourism, Bekol Savannah is object for eco-tourism, that is very attractive for local tourist or foreign and to see biodiversity of flora and fauna. Set of problems are wildlife population very low result presence Acacia nilotica (L) Willd. ex Del in savannah. This plant competitive with wildlife feeding grasslands result to descent plant diversity and biomass. The conclusion of the grassland dispersal at plant of A. nilotica (L) Willd. ex Del. in Bekol savannah Baluran National Park are: (1) The grassland dispersal at plant of A. nilotica (L) Willd. ex. Del.plot 1×1 m2 have 24 species`s with importance value of wildlife feeding grasslands = 40.49%, (2). The grassland dispersal at plant of A. nilotica (L) Willd. ex Del.plot 1x1 m2 (distance of plots 1 m from center point of stem) have 27 species`s with importance value of wildlife feeding = 29.30% and (3). Productivity wildlife feeding grasslands only of to fill 8.93% from wildlife in savannah if be found plant of A. nilotica (L) Willd. ex Del.]]>
<![CDATA[MUTASI TITIK HINGGA MUTASI FRAMESHIFT GEN INSR EXON 22 PADA PASIEN PENDERITA DIABETES MELLITUS ]]>
<![CDATA[Fatchiyah Fatchiyah]]>;
<![CDATA[S. Widyarti]]>;
<![CDATA[I. Mustofa]]>;
<![CDATA[IY. Kusumowardhani]]>;
<![CDATA[R. Fatimah]]>;
<![CDATA[L. Firdausi]]>;
<![CDATA[SP. Ayu]]>;
<![CDATA[Aulanni’am Aulanni’am]]>;
<![CDATA[DW. Soeatmaji]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/248
<![CDATA[Mutations of human insulin and insulin receptor family can lead autosomal dominant syndrome on diabetes, fasting hyperinsulinemia, and insulin resistant. The aim of this research was to identify mutation types of hINSR gene exon 22 which mutation hot spot region. To analyze hINSR gene exon 22 of DM patient and control, we isolated DNA from their blood. DNA was then amplified by PCR using a set of primer for exon 22. PCR product was sequenced by Sequencer and nucleotide sequence analyzed by BLAST analysis. According to Gene Bank database, hINSR gene has two variant with Gene ID 3643, at chromosome 19p13.3-p13.2, and has 22 exons with mRNA 4200bp. The result of research showed that the mutation types of hINS gene exon 22 of DM patients are point mutation, single base deletion and substitution. We found mutation of single deletion at Met1295 Cys1295 and Glut1300Gly1300, also point mutation are at Met1296 Ser1296 and Trp1299Ala1299 and Met1389 Iso1389. Because these two deletion are so close, the polypeptids sequenceof these changed as frameshift mutation, normal IR has six amino acids Met Arg Met Cys ????Trp ????Glut- and DM patient has differed the five amino acids - Cys Ala Ser Ala Gly. According to the mutation of DM patient, the IR protein function against tyrosine kinase become abnormal, perhaps its were correlated with genetic syndrome of insulin resistance.]]>
<![CDATA[VIABILITAS PSEUDOMONAD Fluorescens ISOLAT Pf-122 DALAM BEBERAPA FORMULA ]]>
<![CDATA[Yenny Wuryandari]]>;
<![CDATA[Arika Purnawati]]>;
<![CDATA[Siswanto Siswanto]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/249
<![CDATA[The objectives of this research were to examine effect of several formulas on the viability of Pseudomonas Fluorescens isolate Pf- 122. The eight formulations include carriers, additives, and concentration of Pf-122. The efficacy of various formulation in maintaining the population of Pf-122 in storage was assessed. The result of the viability test of Pf-122 in formula showed that the highest population of Pf-122 in week 0 was observed on the formula 1, viz the formula with combination of pupuk kandang as carrier and CMC as additive and Pf-122 at the concentration of 1010 CFU/ml. The bacterial population of the formula number1 could reach 4.8 × 1010 CFU/formula and followed by the formulas number 2,3,4,5,6, and 7. The lowest population was observed on the formula number 8 with the density of 5.8 × 106 CFU/formula. The bacterial population decreased during storage in all of the formulas and even it could not be detectedin the formulas number 3 and 4 after the first week of storage. In the formulas number 1 and 2 survived within 3 months.]]>
<![CDATA[Pengaruh Variasi Ukuran Biji Terhadap Perkecambahan Acacia Fauntleroyi (Maiden) Maiden and Blakely ]]>
<![CDATA[Mangadas Lumban Gaol]]>;
<![CDATA[J.E.D. Fox]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/250
<![CDATA[The aim of this study was to investigate to what extent are germination of A. fauntleroyi affected by seed size. Does pre-treatment improve germination? Under what temperature regime does most seed germinate? Three seed size classes (small, medium and large) were chosen. Seeds were pre-treatments either at ambient, 50°C, 75°C or 100°C and incubated at 15°C or 30°C. Then, number of seed that germinate and speed of germination were measure. Five seeds representing each of small, medium and large seed sizes were also selected and the seed coat thickness measured. Seed size, pre-treatment temperature and incubation temperature all affected the number of seed that germinated. Pre-treatment temperature affected germination more than incubation temperature. Incubation temperature affected germination more than seed size. The interaction of seed size and pre-treatment temperature was stronger than that between seed size and incubation temperature. Small seeds produce less germination than medium or large seeds, however small seed germinated sooner. Seed coat thickness varied among seed sizes. Thinner seed coats occur in smaller than larger seeds.]]>
<![CDATA[LAMA KEBUNTINGAN MONYET HITAM SULAWESI (Macaca nigra) DI CAGAR ALAM TANGKOKO-BATUANGUS, SULAWESI UTARA ]]>
<![CDATA[Saroyo Saroyo]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/251
<![CDATA[A research about gestational length of Sulawesi crested black macaque (Macaca nigra) has conducted to determine the length between conception and birth. Five adult females of Rambo II Group at Tangkoko-Batuangus Nature Reserve, North Sulawesi, were used as samples. Data collection used focal animal sampling to observe the sex swelling cycle and the time when they gave birth. The length of gestation was determined by counting the time duration between middle fully swollen until giving birth. Data was analyzed descriptively to count the mean and standard deviation. Result showed that the gestational length of Sulawesi crested black macaque was 183.6 ± 11.6 days. From this result can be concluded that the gestational length of this species is longer than other Macaca species.]]>
<![CDATA[KULTUR ANTERA ANTHURIUM: PENGARUH SUKROSA DAN GLUKOSA TERHADAP KEBERHASILAN INDUKSI PEMBENTUKAN KALUS DAN REGENERASINYA ]]>
<![CDATA[Budi Winarto]]>;
<![CDATA[N.A. Mattjik]]>;
<![CDATA[A. Purwito]]>;
<![CDATA[B. Marwoto]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/252
<![CDATA[Effect of sucrose and glucose on callus induction and formation, growth and its regeneration was studied in tissue culture laboratory of Indonesian Ornamental Crops Research Institution from January to November 2009. Objective of this study was to know different combination-concentration of sucrose and glucose on callus induction and formation, growth and its regeneration in anther culture of anthurium. Anthers harvested from spadix with 50% of their pistil in optimal receptive, callus derived from them, Winarto and Rachmawati medium containing 1.5 mg/l TDZ, 0.75 mg/l BAP and 0.02 mg/l NAA were used in the study. Sucrose concentrations were (1) 20 g/l, (2) 40 g/l, (3) 60 g/l dan (4) 80 g/l. While glucose concentrations were (1) 0 g/l, (2) 10 g/l, (3) 30 g/l dan (4) 60 g/l. Variation of the concentrations was applied in callus induction, growth and its regeneration studies. Factorial experiment was arranged by completely randomized design with four replications. Results of this research indicate that existence of sucrose in Winarto and Rachmawati medium gave high effect on successful in antera culture of anthurium and its callus regeneration compared to glucose. Increasing sucrose concentration from 30 g/l to 60 g/l had high impact in callus formation, growth and its regeneration. Sucrose of 60g/l in combination with 30 g/l glucose was the most suitable combination treatment for callus induction and formation with potential growth of antera up to 92%, 67% antera regeneration and 4.0 calluses formed per replication. The sucrose concentration was also tobe the best concentration callus growth and its regeneration with 332 mm3 per callus and 3.2 shoots per explant.]]>
<![CDATA[PERTUMBUHAN TIGA SPESIES JANGKRIK LOKAL YANG DIBudidaya KAN PADA PADAT PENEBARAN DAN JENIS PAKAN BERBEDA ]]>
<![CDATA[Priyantini Widiyaningrum]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/253
<![CDATA[In the development of cricket farming, important information in regard with cultivated method, type of crickets, feedstuff, density level, and it’s productivity is essential. The aim of this study was to evaluate the effects of different density level and rations on growth performace of three species of local crickets, i.e Gryllus bimaculatus De Geer, Gryllus mitratus Burn and Gryllus testaceus Walk. Three levels of box square density (500, 750 and 1000 cricket/box) and two levels of additional feed (mustard green and papaya leaves)were used as treatments. The results showed that the density level of approximately 1000 cricket/box had no significant influence on growth performance of the three species local crickets. Nevertheless, higher density level tended to increase mortality rate especially for G. bimaculatus (rate of mortality = 45.86%). The use of feed combination of formulated ration plus mustard green resulted in better growth performance and higher egg production compared to the combination of formulated ration plus papaya leaves.]]>
<![CDATA[KOMPARASI KERAGAMAN JENIS BURUNG-BURUNG DI TAMAN NASIONAL BALURAN DAN ALAS PURWO PADA BEBERAPA TIPE HABITAT ]]>
<![CDATA[W. Widodo]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/254
<![CDATA[This research was conducted to compare the species diversity of birds in the Baluran and Alas Purwo National Parks (NPs), East Java Province. The research was done on April 2006. The IPA method was used in this bird’s survey. The parameters of the observation were species numbers (S), the total number of each species (n), the total number of all of species (N), the Shannon diversity (H) and the equitability or evenness indices (J), the Simpson diversity (D) and the equitability indices (E). The results showed that Alas Purwo NP has more species numbers than Baluran NP, i.e. 56: 50 species. The highly value of species diversity in the Baluran NP was found on the natural forests habitat, namely 40 species with index values of H’ = 3.27. The highly value of species diversity in the Alas Purwo NP was found on the production plant forests habitat (Tectona grandis), namely 46 species with index values of H’ = 3.33. The Columbidae and Pycnonotidae families were found more abundant in two national parks area. The values of their relative abundance were 17.9% and 11.0%, respectively. By “t” test knows that species diversity (S) have no significant to the H and D diversities indices and the J and E equitabilities indices (P > 0.05). Nevertheless, there appears to be a strong and positive correlation between the species diversity (S) and to the value of H and D indices (P < 0.05, db = 3), with r-value = 0.9204 and r = 0.9031 respectively. In while, the value of S to the E indices are negative correlation highly significant (P < 0.01; db = 3) with r = –0.9785. However, the species diversity value is no correlation significant to J indices with r = –0.3092.]]>
<![CDATA[SPECIES OF THE GENERA TEMORA AND TORTANUS FROM INDONESIAN COASTAL WAT ]]>
<![CDATA[Mulyadi Mulyadi]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/256
<![CDATA[During taxonomic studies on the pelagic copepods of Indonesian waters, three species of Temora, T. discaudata Giesbrecht, 1882, T. discaudata n. var. and T. turbinata (Dana, 1849), and three species of Tortanus, T. (Tortanus) barbatus, T. (Tortanus) forcipatus and T. (Tortanus) gracilis were described and figured on specimens collected from 8 sites along Indonesian coastal waters. Descriptions, measurements and figures are given for those species, along with a review of their distribution over the world oceans, and with taxonomic remarks, ecological notes, and restricted synonymies.]]>
<![CDATA[UMUR GLAS EEL (Anguilla spp.) YANG MASUK MUARA SUNGAI PROGO, YOGYAKARTA ]]>
<![CDATA[Agung Budiharjo]]>;
<![CDATA[Tjut Sugandawaty Djohan]]>;
<![CDATA[Djumanto Djumanto]]>;
<![CDATA[Jusup Subagja]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/334
<![CDATA[The leptocephalus drift with sea currents and moving from spawning area into coastal area near mouth of Progo River. In the coastal area, leptocephalus metamorfosed into glass eel, after that glass eel migrated to river. The aims of this research were to estimate glass eels age and predict hatching dates. Glass eels sampled on new moon during Februari 2007–Mei 2009 at mouth of Progo River. Glass eel ages estimated using their otolith microstructure. Hatching dates predicted with back calculation of glass eels age. We collected 1.082 glass eels. The ages of glass eel at recruit ranged from 58 to 190 days, and divided into 5 age groups. Glass eels are migrated to river hatched on “new moon” from July to January. Glass eels are migrated to river during October–January hatched during July–October. Glass eels are migrated to river during February–Juny hatched during November – January.]]>
