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Sutiman Bambang Sumitro
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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 14 Documents
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Search results for , issue "Vol 18 No 1 (2012): December 2012" : 14 Documents clear
THE N-HEXANE FRACTION OF KESUM (Polygonum Minus L.) INDUCE APOPTOSIS THE LUNG EPITHELIAL CELLS OF THE RATUS NOVERGICUS THAT EXPOSED BY BENZOPYRENE Muhamad Agus Wibowo; Basuki Bambang Purnomo; Mohammad Aris Widodo; Aulanni’am Aulanni’am
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (538.557 KB) | DOI: 10.23869/158

Abstract

Kesum (Polygonum minus L.) is a popular plant and is often used as additives in many typical dishes in the West Kalimantan. These plants belong to the family Polygonaceae, and potential to use in the prevention and treatment of lung cancer. This research aimed at studying the effect of giving the n-hexane fraction of the kesum to induces of apoptosis in epithelial cells of lung tissueof the animal models that exposed by benzopyrene. research was conducted on three groups of animal models: i) the healthy group, ii) the group treated by benzopyrene 200 mg/kg, and iii) the therapy group by n-hexane fraction 100 mg/kg. The results of the study then carried measurements of lung epithelial cell apoptosis index and measurements of lung ROS levels. The results showed that n-hexane fraction able to reduce of lung ROS levels of the animal models that exposed by benzopyrene with MDA levels respectively are 5.09 ± 0.76 ppm for the healthy group, 8.44 ± 1.89 ppm for the group treated by benzopyrene, and 5.47 ± 1.76 ppm for the therapygroup. The n-hexane fraction also able to induce apoptosis of the lung epithelial cells of the animal models that exposed by benzopyrene with apoptosis index values respectively are 2.61 ± 1.98 for the healthy group, 7.41 ± 2.26 for the group treated by benzopyrene, and 16.31 ± 5.73 for the therapy group.
DETEKSI SENYAWA ISOFLAVON DAIDZEIN DAN GENISTEIN PADA KULTUR IN-VITRO KALUS KEDELAI (Glycine Max Merr.) Tintrim Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (972.106 KB) | DOI: 10.23869/159

Abstract

The aim of this research is to identify the isofl avon compounds in the in-vitro cultured callus of soybean (Glycine max Merr.). This is an explorative research, in which callus were cultured in the B5 medium supplemented with 2 ppm 2,4 D. The friable callus were found when it was cultured in the solid medium containing 8 g/l agar and 20 g/l sucrose. When the callus and soybean were extracted with ethanol, a yellow colored substance appeared. If further analysis was done with thin layer chromatography (TLC) method employing 0,2 mm thin layer silica gel 60 F254 (DC-Plastikfolien Schicht-dicke), and eluent consisting n-Butanol - HCL 0.1 N (1:1), six light bluecolor nodes appeared under 366 nm UV light. The nodes have the following Rf: 0,14; 0,30; 0,52; 0,63; 0,79 and 0,92 respectively. This TLC result is comparable with the TLC result from soybeans since they have two nodes with the same Rf and color, namely blue color at Rf 0,81 and 0,92 respectively. Further confi rmation using HPLC (High Performance Liquid Chromatography) equipped with UVvis detector and Lichrospher 100RP–18, (10 μm) colom, as well as Hitachi D–2500 Chromato-integrator indicated that those similar two nodes identifi ed in the TLC were either daidzein or genistein. They can be detected by HPLC at 250 nm and 260 nm, when theywere eluated at the 80% metanol. The HPLC quantitative calculation indicated that concentration of daidzein is four time higher as it was compared with the daidzein concentration in the bean. The concentration of daidzein in the callus remained high up to 4–5 weeks after plantation. It’s concentration will decrease when the callus reached 6 weeks after plantation. Genistein as another component of isoflavon is not appear upon callus, while on soybean seeds extracts, both daidzen and genistein are detected.
EFFECT HYDRAULIC AGITATION USING ANAEROBIC BIOFILTER FOR TREATED DOMESTIC WASTEWATER Nur Indradewi Oktavitri; Agoes Soegianto; Trisnadi Widyaleksono C. Putranto; Angsukma P. Dewayanti; Adam L.H. Suryawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.713 KB) | DOI: 10.23869/160

Abstract

The objective of this study was to investigate the effi ciency the TSS reduction of domestic wastewater by using hydraulic agitation in anaerobic biofi lter. The reactor consists of four columns. Each column has a sampling point. Upfl ow direction was operated in reactor in order to maximize the hydraulic agitation. Gravel was used as fi lter media to support the growth of biofi lm. The reactor treated wastewater of FST Airlangga University canteen which contains 8237.463 mg/l of TSS. Flow used during processing was 0.3 ml/min. Sampling TSS were performed on days 7, 14, 21, and 28. The results showed that the effi ciency of TSS were 64.57%, 76.16%, 94.23% and 98.46% respectively. The average TSS removal was 83.36%.
ANALISIS LAJU TIMBUNAN DAN KOMPOSISI SAMPAH DI PERMUKIMAN PESISIR KENJERAN SURABAYA Nita Citrasari; Nur Indradewi Oktavitri; Nuril A. Aniwindira
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.66 KB) | DOI: 10.23869/161

Abstract

Solid waste in Coastal Settlement Kenjeran Surabaya is alarming due to of the habit of the peoples to pile until 1 meter on surface coastal land. The habit can impact of environmental pollution to that area. So, the purpose of this research was to know the solid waste generation and the composition of solid waste in Coastal Settlement Kenjeran Surabaya. The methods used was weigth volume analysis. That result of research showed that the solid waste generation was 0.23 kg/person/day with the composition 76.21% of garbage, 10.83% of plastic, 5.33% of paper, 0.82% of glass, 1.21% of wood, 2.27% of fabric, 0.44% of metal, 0.08% of foam, and 2.58% of the others(soil, sand, and gravel). The solid waste generation and the composition of solid waste could be used for the planning of management in Coastal Settlement Kenjeran Surabaya.

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