cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Zahratul Aini
Contact Email
dr.zahratulaini@unsyiah.ac.id
Phone
+62812388847262018
Journal Mail Official
m.zainudin@uii.ac.id
Editorial Address
Jl. Kaliurang Km 14,5 Yogyakarta, Indonesia
Location
Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Published by Universitas Islam Indonesia
ISSN : 20854145     EISSN : 25272950     DOI : 10.208885/JKKI.Vol10.Iss3.art5
Core Subject : Health,
Medicine & Pharmacology Public Health
JKKI: Jurnal Kedokteran dan Kesehatan Indonesia is a peer-reviewed journal in the field of medical and health sciences. This journal is designed as a place of dissemination of information and scientific knowledge, which publishes three times a year. It publishes original article, article review, and case report. These comprise of biomedical sciences, clinical medicine, public health sciences, and medical science education.
Arjuna Subject : Kedokteran - Kedokteran Keluarga
Articles 13 Documents
 1   2 
Search results for , issue "JKKI, Vol 11, No 3, (2020)" : 13 Documents clear
Microbial approach of epitope tagged MFE-23 single fragment antibodies production Razmaeda Sarastry
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art3

Abstract

Background: Antibodies have been investigated for future clinical application in cancer management. An antibody, MFE-23 scFv is known for its ability to bind Carcinoembryonic Antigen (CEA). Different from a full length antibody, single-chain variable fragments (scFvs) are recombinant antibodies in which single polypeptide is engineered to replace variable regions encoding antigen-binding domain. In vitro production of single chain fragment antibodies may use E. coli microorganism for its ability to self-replicating a plasmid. Objective: This study aimed to produce his- and myc- tagged MFE-23 scFv antibodies by using E. coli culture and to detect their solubility by using ELISA assay. Methods: Transformed E. coli containing sequences of MFE-23 coding were inoculated and evaluated for their optical density. An ELISA plate was coated by CEA or PBS and secondary antibodies were anti-his, anti-myc and anti-MFE. Horseradish peroxidase-OPD substrate was added to produce chromatic reaction for qualitative detection. Results: The results showed that each characterized tube was positive for myc-tagged MFE, his- and myc- tagged MFE, and his-tagged MFE for tube 1, 2, and 3 respectively.Conclusion: This study indicated that transformed E. coli culture is a suitable host for MFE-23 svFV production, and qualitative ELISA assay is a simple useful method for antibody detection and characterization of single chain antibodies.
PERBEDAAN MORFOLOGI DAN FRAGMENTASI DNA PADA SPERMATOZOA TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR PASCA INDUKSI BERBAGAI MODEL STRES SLEEP DEPRIVATION Norina Agatri; Fitranto Arjadi; Lantip Rujito
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art4

Abstract

Background: A stress, sleep deprivation, can cause imbalance of reactive oxygen species (ROS) that can damage DNA, lipids and proteins of spermatozoa, consequently leading to infertility.Objective: The aim of this study is to investigate differences of morphology and DNA fragmentation with some models of sleep deprivation in spermatozoa of white male Wistar rat strain (Rattus novergicus).Methods: This study was an experimental study with post-test-only with control group design. There were 30 male Wistar rats which were randomly divided into 5 groups: control group, paradoxical sleep deprivation (PSD) group, total sleep deprivation (TSD) group, sleep recovery (SR) group, PSD group + SR group, and TSD group + SR group. Stress induction was conducted for 5 days. Morphological abnormality of spermatozoa was observed by creating sperm removal tinged by eosin and nigrosine. DNA fragmentation was performed by determining DNA fragmentation index (DFI). Morphological data of spermatozoa were analysed by One-Way Annova and Post-Hoc Turkey test, and DNA fragmentation of spermatozoa was analysed by Kruskal Wallis and Mann Whitney test to determine differences among the groups.Results: The highest mean of the abnormal morphology was in PSD Group (89.5 %, p=0.004). The normal mean of DNA fragmentation was found in control group, PSD+SR group and TSD+SR group, PSD and TSD groups. Bad DNA fragmentation index was found in PSD and TSD groups (30.16% and 31.4%, p=0.023).Conclusion: There were differences of morphology and DNA fragmentation of the spermatozoa in the male Wistar rat strain induced by various models of sleep deprivation.
The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas Metta Ayu Susanti; Gembong Satria Mahardhika; Lantip Rujito; Anton Budhi Darmawan; Dwi Utami Anjarwati
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art7

Abstract

Background: Methicillin-Resistant Staphylococcus aureus (MRSA) has become a major pathogene and its incidence continues to increase in various countries. The resistance to methicillin is caused by the mecA gene that encodes the expression of Penicillin Binding Protein 2a (PBP2a) so it has a low affinity for beta-lactam antibiotics. One of the factors that can increase the incidence of MRSA is inappropriate antibiotics use. Objective: This study is to identify presence of the mecA gene in MRSA and to compare histories of inappropriate antibiotic uses of healthcare workers and communities in Banyumas.Methods: This was a cross-sectional study conducting 120 samples from nasal swabs. Isolates of MRSA tested by bacteriological examinations and PCR of the mecA genes. Histories of antibiotic uses were assessed by using questionnaires and then analysed descriptively by using Fisher Exact test (SPPS, version 20).Result: One sample of S. aureus from the microbiology examination (0.83%) was an MRSA although there was no a mecA gene identified by using the PCR method. 66.1% of the healthcare workers and 88,3% of the communities used antibiotics inappropriately. The results showed that there was a statistically significant difference in the histories of irrational uses of antibiotics (p?0.05).Conclusion: A number of the communities who used inappropriate antibiotics was higher than the health workers. Furthermore, one MRSA strain with a negative mecA gene was found in the health worker group.
Fahr’s syndrome in teenage girls with systemic lupus erythematosus: A case report Damar Prasetya; Sumadiono Sumadiono
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art11

Abstract

Fahr’s disease or Fahr’s syndrome is a condition characterized by intracranial calcifications especially in basal ganglia and dentate nuclei. They are a rare condition with a broad range of manifestations from asymptomatic condition to severe neurological and psychiatry signs and symptoms. The aim of this report to present Fahr’s syndrome as an alternative diagnosis of neuropsychiatry symptoms in systemic lupus erythematosus (SLE) patients. We present a 17-year-old teenage girl who have had a preexisting diagnosis of SLE and have been on remission. The patient recently had experienced headache, auditory hallucination and memory loss despite having a low activity score of SLE and no laboratory relapse. Head CT scan showed bilateral basal ganglia calcifications suggesting a Fahr’s disease. Clinical outcome in this condition might vary but might worsen neurocognitive functions. 
Primary repair of tetralogy of fallot and major aorto-pulmonary collateral arteries with suspected Noonan syndrome: A rare case Heroe Soebroto; Erdyanto Akbar; Arief Rakhman Hakim
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art13

Abstract

Tetralogy of Fallot (TOF) is a congenital heart disease that includes ventricular septal defect (VSD), pulmonary stenosis (PS), right ventricular hypertrophy (RVH), and an overriding aorta. A cyanotic heart defect can develop aorto-pulmonary collateral (APCA) to supply underperfused pulmonary beds. Genetic syndromes can occur in 20% of patients with TOF. In developing countries, diagnosed genetic syndromes associated with heart defects have not been covered by health insurance, even though outcomes of primary repair remain unknown. This study reported a case of an 11-year-old boy with cyanosis since birth, dyspnoea on effort,  growth delays, learning problems, scoliosis,  micropenis, hypertelorism, exophthalmos, facial dysmorphism, cryptorchidism, and major aorto- pulmonary collaterals. The patient underwent an echocardiographic study. It showed VSD malalignment with overriding aorta of 48%, critical pulmonary stenosis, and size of pulmonary arteries that were favourable for primary repair. Cardiac catheterization showed classic Fallot with normal coronary arteries and major aortopulmonary collateral arteries (MAPCA) arising. The patient underwent percutaneous coil embolization just before surgery. There was a problem during induction, the anaesthetist found that it was hard to place the ETT because there was membrane-like covering the trachea. Next, a median sternotomy was conducted, and aortic cross-clamping was achieved. Right atriotomy was performed, and it provided exposure to the malalignment VSD. A longitudinal pulmonary arteriotomy was made to expose infundibular stenosis. Infundibulectomy was performed, and it reconstructed the MPA with a pericardial patch. The VSD was closed by using a PTFE patch. The patient was transferred to an ICU afterward. Post-operative echocardiography showed a good outcome, and 15 days after surgery the patient was discharged. It could be concluded that primary repair of TOF with MAPCAs and suspected Noonan syndrome was conducted successfully.
Effects of various lethal doses of amitriptyline to the length of Calliphoridae larvae Emira Alifia; Arfi Syamsun; Eustachius Hagni Wardoyo
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art8

Abstract

Background: Uses of insects to predict Post-mortem Interval (PMI) is important for non-natural causes of death such as drug abuses. Doses of drugs in a corpse are presumed to affect rates of growth and development of insects and relationships to predict the PMI. Objective: This study aims to study effects of various doses of lethal amitriptyline to the length of Calliphoridae larvae stage as the first organism seen on a corpse as well as to assess other factors involved in insect growth such temperatures and humidity.Methods: This study was an experimental research using 24 dead rats as larva’s growth media. These rats were divided into four group: the control group, the first treatment (T1) group, the second treatment (T2) group, and the third treatment (T3) group. The control group was deceased by cervical dislocation representing natural cause of death, while the treatment groups were given various doses of amitriptyline orally. The doses given to T1, T2, and T3 groups were 75 mg, 100 mg and 125 mg, respectively. Next, the rat corpses were put into cages containing Calliphoridae larvae; daily observation was conducted every morning and afternoon until the larvae transformed into pupae. All obtained data were analysed by using a multivariate linear regression analysis, Spearman correlation and Kruskal-Wallis.Results: This study showed that the more doses of amitriptyline, the longer larva cycle and the shorter length of larvae (p<0,05). The life cycle time in the control, T1, T2, and T3 groups were four, five, eight and nine days, respectively. Based on the first day of larva appearance in each group, the control group produced an average length of larvae longer than the T1, T2, and T3 groups, respectively 8.33 mm, 7.33 mm, 4.5 mm and 5.67 mm. However, differences of temperatures and humidity observed in the routine morning and afternoon did not have any differences.Conclusion: Increasing more doses of amitriptyline extended the larva cycle that could cause the larva length to be shorter in the treatment group compared to the control group on the same day. Environmental factors in this study had smaller effects on the larva length growth of the Calliphoridae larvae.
Nurses’ experiences in caring for dying patients: A literature review Agus Ari Pratama; Shanti Wardaningsih
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art10

Abstract

Every human will face death, and some patients can experience a critical condition during illness before death. Nurses are medical professionals who are expected to be able to care for a patient's critical condition. Studying experiences of nurses in dealing with a patient's critical condition is needed so that they can improve their services. This study aims to analyze articles that explain about nurses' experiences in dealing with dying patients. A literature review was conducted by searching articles from the PubMed and EBSCO databases (using keywords "dying AND nurse AND experience"). In the PubMed databases were found 563 articles, and in the EBSCO were found 1,632 articles. From the all the databases, only 10 articles met the inclusion and exclusion criteria. Results of the article reviews found that the experiences of nurses in caring for dying patients suggest nurses to have more empathy and to provide contextual moral encouragement for patients and their families. Nurses also need to prepare themselves with knowledge about end of life.
Characterization of pili protein with molecular mass of 85 kDa Escherichia coli as an adhesin and a hemagglutinin Dini Agustina; Siti Marissa Aisyah; Ika Rahmawati Sutejo; Diana Chusna Mufida
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art5

Abstract

Background: Escherichia coli (E. coli) is a rod-shaped gram-negative bacterium included in Multi Drug Resistant (MDR) bacteria. There are several strategies in place to prevent and treat E. coli bacterial infections to reduce some the incidences of MDR. One of them is the development of a protein-based vaccine. Pili is one of virulence factors in E. coli surface proteins that can mediate attachments of bacteria to host cells (adhesin or hemagglutinin) and can be used as protein-based vaccine candidates. Objective: This study aims to determine characterization of pili protein with molecular mass of 85 kDa Escherichia coli bacteria as an adhesin and a hemagglutinin. Methods: This study is a true experimental laboratories and a descriptive study to determine roles of pili protein with molecular mass of 85 kDa as an adhesin and a hemagglutinin. Samples used in this study were a stock of E. coli in the Microbiology Laboratory Faculty of Medicine, Universitas Negeri Jember (UNEJ). A hemagglutination test in this study used mouse erythrocytes while an adhesion test used mouse enterocytes. Isolation and purification of E. coli pili protein, isolation of mouse erythrocytes, isolation of mouse enterocytes, the hemagglutination test and the adhesion test were methods in this study. A data analysis with a correlation-regression was used to determine relationships between the adhesion index and titer pili 85 kDa with a limit of significance, 0.05 (p <0.05). Results: The hemagglutination test showed no ability of agglutination with the formation of red aggregate points starting from the first dilution. An analysis with Pearson correlation indicated a relationship between titer pili 85 kDa E. coli and adhesion index (p = 0.009; R = -0.921).Conclusion: Pili protein 85 kDa E. coli plays a role as an adhesin protein and do not have a role as a hemagglutinin protein.
Prolonged severe acute respiratory syndrome of Corona Virus-2 positive with polymerase chain reaction: A case report Linda Rosita; Rahma Yuantari; Budi Mulyono; Umi Solekhah Intansari; Ingesti Bilkis Zulfatina
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art12

Abstract

COVID-19 continues to spread across the world, with more than 60 million confirmed cases in 220 countries. Since March 11, 2020, WHO announced the COVID-19 outbreak as a pandemic. Impacts caused by COVID-19 are not only on the health sector but also economic, social, and political sectors. In a diagnosing process, the SARS-CoV-2 PCR results for swab specimen are a gold standard in determining confirmed COVID-19 cases. Previously, 2 repeated results of negative SARS-CoV-2 PCR become criteria of being recovered from COVID-19. Therefore, based on the latest guidelines from the Indonesian Ministry of Health revised on July 13th 2020, patients with no symptoms, mild to moderate symptoms, and severe symptoms are declared to be recovered if the patients meet the requirements of finished periods of isolation based on a doctor’s assessment. Confirmed cases with severe or critical symptoms are specially declared with one negative result of the PCR test plus three days without showing any symptoms. This case study discussed a 52-years-old woman diagnosed with COVID-19. PCR test for this patient had been conducted 11 times during 64 days of treatment, and its results turned to be always positive, failing to fulfil the discharge criteria. Therefore, it needs to determine causes of prolonged positive PCR results and impacts of clinical condition of the patients, and prognosis of the patients.
Public knowledge of self-medication in Ngaglik subdistrict of Sleman regency Dian Medisa; Fithria Dyah Ayu Suryanegara; Ditya Ayu Natalia; Puspita Fitri Handayani; Dhea Putri Indra Kusuma; Diesty Anita Nugraheni
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art6

Abstract

Background: Self-medication is using medicines without a prescription or health professional advice. Over-the-counter (OTC) medicines are commonly used in self-medication. Knowledge and practices of self-medication is required to be assessed to ensure appropriate uses of medicines.Objective: This study is to examine public knowledge and practices of self-medication in Subdistrict of Ngaglik, Sleman, D.I. Yogyakarta.Methods: A cross-sectional survey was conducted in three villages in Ngaglik, Sleman. 339 participants were selected through a cluster sampling. Data were collected by using a self-administered questionnaire. Descriptive analysis and chi-square test were used to analyse the data.Results: Most of participants were female (61.7%) aged between 26-45 years (49.9%). More than fifty percent of participants (59%) had education level of senior high school, and 49.9% participants had good knowledge of the OTC self-medication. The level of knowledge of self-medication was significantly associated with the level of education (p values<0.05). The most commonly used OTC medicines for self-medication were analgesics and antipyretics (48.4%), respiratory drugs (29.7%), and gastrointestinal drugs (9.6%). OTC medicine advertisements were common sources of information about medicines for self-medication (39.5%) whereas information sources from health professionals were accounted for only 2.4%.Conclusion: The public knowledge of self-medication needs to be developed. Health professionals, especially pharmacists, are suggested to provide more information on the OTC medicines for public to allow them to access additional information sources than medicine advertisements.

Page 1 of 2 | Total Record : 13

 1   2 

Filter by Year

2020 2020


Reset
Filter By Issues
All Issue JKKI, Vol 16, No 3, (2025) JKKI, Vol 16, No 2, (2025) JKKI, Vol 16, No 1, (2025) JKKI, Vol 15, No 3, (2024) JKKI, Vol 15, No 2, (2024) JKKI, Vol 15, No 1, (2024) JKKI, Vol 14, No 3, (2023) JKKI, Vol 14, No 2, (2023) JKKI, Vol 14, No 1, (2023) JKKI, Vol 13, No 3, (2022) JKKI, Vol 13, No 2, (2022) JKKI, Vol 13, No 1, (2022) JKKI, Vol 12, No 3, (2021) JKKI, Vol 12, No 2, (2021) JKKI, Vol 12, No 1, (2021) JKKI, Vol 11, No 3, (2020) JKKI, Vol 11, No 2, (2020) JKKI, Vol 11, No 1, (2020) JKKI, Vol 10, No 3, (2019) JKKI, Vol 10, No 2, (2019) JKKI, Vol 10, No 1, (2019) JKKI, Vol 9, No 3, (2018) JKKI, Vol 9, No 2, (2018) JKKI, Vol 9, No 1, (2018) JKKI, Vol 8, No 3, (2017) JKKI, Vol 8, No 2, (2017) JKKI, Vol 8, No 1, (2017) JKKI, Vol 7, No 5, (2016) JKKI, Vol 7, No 4, (2016) JKKI, Vol 7, No 3, (2016) JKKI, Vol 7, No 2, (2015) JKKI, Vol 7, No 1, (2015) JKKI, Vol 6, No 4, (2015) JKKI, Vol 6, No 3, (2014) JKKI, Vol 6, No 2, (2014) JKKI, Vol 6, No 1, (2014) JKKI, Vol 5, No 2, (2013) JKKI, Vol 5, No 1, (2013) JKKI, Vol 3, No 8, (2011) JKKI, Vol 2, No 6, (2010) JKKI, Vol 2, No 5, (2010) JKKI, Vol 1, No 3, (2009) JKKI, Vol1, No 1, (2009) More Issue