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DENTA JURNAL KEDOKTERAN GIGI Jl. Arief Rahman Hakim No.150, Keputih, Kec. Sukolilo, Kota Surabaya, Jawa Timur 60111 Gedung F6 Fakultas Kedokteran Gigi Telp. 031-5945864
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Denta: Jurnal Kedokteran Gigi
Published by Universitas Hang Tuah
ISSN : 19075987     EISSN : 26151790     DOI : https://doi.org/10.30649/denta.v16i1
Core Subject : Health,
Dentistry
The Denta (Jurnal Kedokteran Gigi) accepts original manuscripts relating to the field of dentistry, including: research reports, case reports and literature reviews. The spread of dental fields comprise: -Dental material -Endodontics -Forensic dentistry -General dentistry -Oral and maxillofacial surgery -Oral biology -Oral medicine -Oral pathology -Orthodontics -Pediatric dentistry -Periodontics -Prosthodontics -Public health dentistry -Radiographic dentistry -Biomarine Science in Dental field
Arjuna Subject : Kedokteran Gigi - Kedokteran Gigi (Lain-Lain)
Articles 13 Documents
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Search results for , issue "Vol 9 No 1 (2015): Februari" : 13 Documents clear
Aplikasi Gel Kitosan Berat Molekul Tinggi dan Rendah terhadap Ketebalan Epitel Mukosa pada Proses Penyembuhan Luka Pencabutan Gigi Fransiska Amelia Hartono; Puguh Bayu Prabowo; Syamsulina Revianti
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Acceleration of wound healing after tooth extraction is the most important. One of the tissue repair in wound healing is the reepithelialization. Chitosan is a biomaterial that can be used to accelerate reepithelialization in the wound healing process. Purpose: The aim of this study was to prove the differences between application of chitosan gel high molecular weight and low molecular weight to accelerate the reepithelialization in socket healing after tooth extraction. Material and Methods: The experiment was held the post test only control group design used 24 male wistar rats divided into 3 group. K1 group was control groupwhich was extracted without any treatment, K2 group was extracted and applicated by low molecular weight of chitosan gel, K3 group was extracted and applicated by high molecular weight of chitosan gel. After treatments on the 7 day, all groups of rats were euthanized and the epithelial mucosal thickness was measured under light microscope magnificant 100x. All of the data were analyzed by one way ANOVA and LSD test. Result: This study showed the epithelial thickness of the K2 and K3 groups was significantly higher than K1 group, but there were not significantly different between K2 and K3 group. Conclusion: The effectivity of high molecular and low molecular weight chitosan gel to accelerate reepithelialization in socket healing are similar.
Daya Antibakteri Ekstrak Daun Beluntas (Pluchea Indica Less) Terhadap Streptococcus Viridans (In Vitro) Agni Febrina Pargaputri; M. Mudjiono; Agus Subiwahjudi
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Pluchea indica Less leaves is a species of plants that has several chemical properties. It consists of flavonoids, tanine, and essensial oil which had been reported as antibacterial agents. Streptococcus viridans is the most common bacteria found in infected root canal teeth, and the extract of Pluchea indica Less leaves could be potentially used as one of root canal sterilization dressing in root canal teeth. Purpose: The aim of this study was to determine antibacterial activity and Minimum Bactericidal Concentration (MBC) of Pluchea indica Less leaves extract against Streptococcus viridans. Materials and Methods: The antibacterial testing of Pluchea indica Less leaves extract was performed using dilution method against Streptococcus viridans. The tube with concentration extract of 25% was showed no visible turbidity, while the tube with concentration extract of 12,5% was showedgrowth of Streptococcus viridans. We made range between concentration 12,5% until 25% to determine the Minimum Bactericidal Concentration (MBC) of Pluchea indica Less leaves extract. The result of antibacterial activity of Pluchea indica Less leaves extract was determined by measure the growth of Streptococcus viridans using colony count. Result: The result showed that Pluchea indica Less leaves extract was able to inhibit the growth of Streptococcus viridans on concentration 12,5%-25%. The results of Anova statistical test (significant value <0,05) showed significant different among each concentration of Pluchea indica Less leaves extract. Conclusion: Pluchea indica Less leaves extract had antibacterial activity against Streptococcus viridans. Minimum Bactericidal Concentration of Pluchea indica Less leaves extract was performed in concentration 12,5%-25%, which was could kill more than 99% growth of Streptococcus viridans.
Efektivitas Kombinasi Grafting Cangkang Kerang Darah (Anadara granosa) dan Minyak Ikan Lemuru (Sardinella longiceps) Terhadap Penurunan Jumlah Osteoklas Pada Proses Bone Repair Dinda Divilia; Rima Parwati Sari; Paulus Budi Teguh
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: In dentistry, complication post-extraction, disease periodontal, prosthetic surgery, pathological conditions, and trauma can cause alveolar bone loss and even lead to tooth loss. The use of hydroxyapatite derived from blood cockle shells is osteokondiction. Omega-3 in sardine oil will stimulate the healing process of damaged bone with decreasing in the number of the osteoclasts. Purpose: The aimed of this study to determine the Effectivity of Grafting Combination Blood Cockle Shell and Sardine Fish Oil to Decrease in The Numberof Osteoclasts in The Process of Bone Repair Methods: Animals used in research, as many as24 male Wistar rats, which then divided into 4 groups, namely the control group and the treatment group. The control group subdivided into two groups, normal healing and human application's paste. In the treatment group, also divided into two groups, group with blood cockle shell application and combination of blood cockle shell and sardine oil. Osteoclasts viewed using HPA on the 7th day. Results: Decrease in The Number of Osteoclasts on K- group : 2.67±1,033, P1 : 2.33±0.09, K+ : 1.5±0.09, P2 : 0.83±0.54. Because the data not homogeneous then used test Kruskal-Wallis test and Mann-Whitney. P1 with P2 have a difference significantly compared 0,048 , P2 with K- 0,026 (p<0,05). Conclusions: Combined administration of blood cockle shell (Anadara granosa) and sardine oil (Sardinella longiceps) significantly influence the amount of osteoclasts in the process of bone repair on the 7th day.
Daya Hambat Ekstrak Rumput Laut Spesies Eucheuma Cottonii Terhadap Pertumbuhan Bakteri Mixed Periodontopatogen Iqbal Fahmiliyan Asmoro; Yoifah Rizka; Paulus Budi Teguh
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Bacterial mixed periodontopatogen dominated by gram negative. Eucheuma cottonii seaweed as one of the plant species have the antibacterial effect againts gram positive and gram negative bacteria.thus potentially developed as antibacterial agent in periodontal disease. Purpose: This study aimed to determine the inhibition effect of Eucheuma cottonii seaweed extract to the growth of mixed periodontopathogen bacteria concentration of 0,25%, 0,5%, 1% and 2%. Materials and Methods: Eucheuma cottonii seaweed extracted with 95% etanol by soxhlet method. Mixed periodontopathogen bacteria inoculated in BHI medium. Antibacterial effects of extracts of Eucheuma cottonii seaweed againts bacterial mixed periodontopathogen was tested by the diffusion method with the concentrations of 0,25%, 0,5%, 1%, 2%, aquadest and tetracycline, where each group consisted of 5 experiment unit. The effect of the inhibition observed by measuring the diameter of clear area on the disk using digital calipers stated in millimeters. Result: There were inhibition effect at each concentrations of treatment. The diameter zone were concentration of 0.25% (6.184), the concentration of 0.5% (6.356), the concentration of 1% (6.62), the concentration of 2% (7.148) and a positive control with tetracycline (39.336) Conclusion: Eucheuma cottonii seaweed extract has inhibition effect to the growth ofbacteria mixed periodontopatogen.
Daya Hambat Ekstrak Teripang Emas (Stichopus hermanii) terhadap Bakteri Enterococcus faecalis Roy Tamara; Linda Rochyani; Paulus Budi Teguh
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Enterococcus faecalis is one caused bacteria of root canal infections. ChKM is mostly used as sterilization agent in endodontic treatment. Stichopus hermanii extract has been reported to have antibacterial effects for gram-negative and gram-positive bacteria, so could be potentially developed as a root canal sterilization agent. Purpose: The aim of this study was to determine the inhibitory effect of Stichopus hermanii extract on Enterococcus faecalis bacteria. Materials and Methods: This study was an experimental study with post test only control group design and were tested by diffusion methods with 4 groups concentration of 2,5%, 3%, 3,5%, 4%, and 2 controls groups using DMSO 1% as negative control, and ChKM as positive control, each group consisted of 7 samples. The inhibition effect were examined by measure the diameter of the clear zone around the disc. Data were analyzed by one way ANOVA test and followed by LSD test. Results: Results showed that the mean of inhibition zone at concentrations of 2,5% (7,3543 mm), 3% (8,1086 mm), 3,5% (8,7286 mm), 4% (9,4029 mm), DMSO 1% (6,0257 mm) and ChKM (25,49 mm). It had been proved that. Stichopus hermanii extract could inhibit the growth of Enterococcus faecalis (p<0,05). The largest diameter of the clear zone was in the concentration of 4%. Conclusion: Stichopus hermanii extract could inhibit the growth of Enterococcus faecalis and the most effective inhibitory concentration is 4%.
Daya Hambat Ekstrak Teripang Pasir (Holonthuria scabra) Terhadap Pertumbuhan Bakteri Mixed periodontopatogen In Vitro Susi Krestiana; Widyastuti; Vivin Ariestania
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Periodontitis is caused by the occupation of gram-negative bacteria (mixed periodontopatogen) in oral cavity. Despite causing resistance, antibiotics are still used as a therapy for periodontitis. Sand sea cucumber (Holonturia scabra) extract hold somepotentials, if developed, to become the alternative therapy for periodontitis due to its antibacterial effects against gramnegative bacteria. Purpose: To determine sand sea cucumber extract (Holonturia scabra) in inhibiting the growth of mixed periodontopatogen bacteria. Materials and Methods: This study used a research design the post-test only control group design. 40 samples of BHI agar plates, contained mixed periodontopatogen bacteria samples, were divided into 5 groups samples. K1 (negative control) was given distilled water containing filter paper, K2 (positive control) was given the antibiotic tetracycline disc 30 g, while P1, P2, and P3 were given a filter paper containing sand sea cucumber extract with a concentration of 150 g/ml, 300 g/ml, and 450 g/ml. All sample from each group were anaerobically inoculated for 2x24 hours. After inoculation, the area aroundthe filter paper will appear to be clear and it will be measured with digital calipers (mm). The acquired data will be analyzed using the Kruskal-Wallis and Mann-Whitney test. Result: The inhibition rate of K1 (6,00 ± 0,00), K2 (28,975 ± 1,20049), P1 (6,8525 ± 0,30156), P2 (7,6237 ± 0,51281), and P3 (8,75 ± 0,3525). Conclusion: Sand sea cucumber extract (Holonthuria scabra) can inhibit the growth of Mixed periodontopatogen bacteria.
Efek Pemberian PRF dengan Xenograft dan Alloplast terhadap Jumlah Osteoblas Hansen Kurniawan
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Bone graft materials have been widely used to regenerate bone defects, which are caused by trauma, tumor resection, pathological processes, and congenital bone defects. Type of bone graft is classified into several types, autograft, allograft, xenograft, and alloplast PRP and PRF derived from autologous blood which sends a high concentration of growth factors on bone defect area. Purpose: The aim of this study is to investigate the effect of PRF with xenograft and alloplast to osteoblast in bone New Zealand rabbits. Materials and Methods: The experiment was held by Post Test Group design. Twenty seven male New Zealand Rabbits were divided into three group. The first group performed the treatment on the right hind limb be treated with Alloplast and PRF treated, The second group performed the treatment on the right hind limb be treated with xenograft and PRF, and The third group was the control group performed the treatment on the right hind limb was given PRF. After treatment the rabbits were sacrificed. Osteoblast of each group was measured by EDTA method All data experiment were analyzed by ANOVA and LSD test (p<0,01). Results: The result of this study showed that the results of osteoblasts between PRF with alloplast compared with prf with xenografts, significant differences as a result of the process of bone formation to see the results of better bone formation. Conclusion: In PRF with alloplast has better results compared with PRF with xenografts from to increase osteoblasts expression.
Efektifitas Perbandingan Kombinasi Clindamycin dan Ekstrak Nannochloropsis Oculata Terhadap Peningkatan Kepadatan Kolagen pada Osteomielitis Mandibula Faidha Azmi N; Dian Mulawarmanti; Noengki Prameswari
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Osteomyelitis is an bone infection caused by bacteria of Staphylococcus aureus. The use of clindamycin within long term may cause of resistance. A compound in Nannochloropsis oculata’s extract contain antibacterial, antiinflamation, antioxidant that can be used as therapy of osteomyielitis. Purpose: Knowing comparison of the effectiveness between the combination of clindamycin and Nannochloropsis oculata’s extract to increasing the density of collagen on the mandibular osteomyelitis. Materials and Methods: The experimental research’s type with a design post test only control group. Fourty male wistar rats as model of mandibular osteomyelitis has made by inducing bacteria of Staphylococcus aureus inserted in socket after an extraction of left incisive teeth on lower jaw and waiting for 28 days. The rats are divided into 5 group: negative control (K1), clindamycin (K2),clindamycin with 50mg Nannochloropsis oculata’s extract (K3), clindamycin with 100mg Nannochloropsis oculata’s extract (K4), clindamycin with 200mg Nannochloropsis oculata’s extract. After 7 days, all of group are euthanazed and then the density of collagen measured using histopathology slide used Masson’s Trichom and 100x enlargement, so then analyzing the density of collagen by Kruskal-Wallis and Mann-Whitney. Result: There were significant differences in collagen between K1 and K2 (p=0,037), K1 and K3 (p=0,013), K1 and K4 (p=0,002), K1 and K5 (p=0,000). and there were significant differences in collagen between K2 and K5 (p=0,013). But there were no significant differences in collagen between group K2 and K3 (p=1,000), K2 and K4 (p=0,288). Conclusion: The combination of clindamycin with 200mg Nannochloropsis oculata’s extract having an effect on increasing number of the density of collagen significantly on the healing of mandibular osteomyelitis.
Karakterisasi Esktrak Air Teripang Emas (Stichopus hermanii) Dian Widya Damaiyanti
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Indonesia is the country with the biggest sea cucumbers world’s producer. So far, sea cucumbers are only used as a side dish alone. Indonesia can provided added value and high economic value of gold sea cucumber through right procedure. A lot of gold sea cucumbers active substance that are suspected to have an influence on wound healing. For the best result, extract gold sea cucumber must be prepare with correct procedure. Water extract is one of a safe procedure with a small risk that may damage parts of the active substance. Purpose: The aim of this study is to know the characterization of water extract gold sea cucumber (Stichopus hermanii). Materials and Methods: Gold sea cucumbers were collected, homogenized with distillated water and shaken with water-bath shaker. Then extract was freeze dried, the result then characterized using spectrophotometer. Result: Thelargest component to fewest component of water extract gold sea cucumber is total protein 76,82%, essential amino acid 48,11%, non essential amino acid 28,70%, glycoprotein 4,62%, collagen 4,05%, GAG’s 1,62%, proteoglycan 1,13%, heparin sulfat 1,02%, calcium 59%, saponin 56%,heparin 38%, hyaluronat acid 29%, calcium 0,59%, Zinc 0,01%. Conclusion: There is many active substance of water extract gold sea cucumber that involed in wound healing process.
Pengaruh Teripang Emas (Stichopus Hermanii) pada Remodeling Ekspansi Sutura Maksila Terhadap Lebar Palatal Menggunakan Analisis Sefalometri Anydya Putri; Noengki Prameswari; Budi Handayani
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Maxillary expansion device causing mechanical pressure to open the maxillary suture which causes inflammation and bone remodeling around the area. Stichopus hermanii is known to contain flavonoid, chondroitin sulfate, omega 3 (EPA and DHA) and dermatan sulfate which is used to inhibit inflammatory process, increase bone metabolism and mineralization, and bone remodelling. Purpose: The aim of this experiment is to know the effect of Stichopus hermanii on maxillary suture remodeling expansion to palatal width using cephalometric analysis. Materials and Methods: Experimental units used was 24 male Caviacobaya divided into 4 groups (n=6). Group K(-) is negative control. Helical spring was applied to and activated in the other 3 groups for 10 days. Group K(+) was given 2% NaCMC gel, both group P1 and P2 was given 3% Stichopus hermanii gel. Group P2 werealso given retention period for 10 days after activation period. Cavia cobaya were then decaputated and observed for the palatal width changes. The data was analyzed with One Way ANOVA test continued with LSD test. Result: The result of ANOVA test showedsiginficant differences among the treatment groups p=0,000 (p<0,05). LSD test showed significant differences between group K(-) and K(+) (Sig. 0,000), K(-) and P1 (Sig. 0,000), K(-) and P2 (Sig. 0,000), K(+) and P2 (Sig. 0,020) and group P1 and P2 (Sig. 0,036).Conclusion: 3% Stichopus hermanii gel has effect on maxillary suture remodeling expansion to palatal width use cephalometric analysis and combination of Stichopus hermanii with 10 6days retention period resulting the widest palatal width.

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