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Jurnal Sain Veteriner
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Arjuna Subject : -
Articles 17 Documents
Search results for , issue "Vol 18, No 1" : 17 Documents clear
HISTOLOGY AND ELECTRONE MICROSCOPIC STUDY OF THE EFFECTS OF AN GnRH AGONIST ON THE CANINE TESTIS AND PROSTATE AFTER 3 MONTHS OF TREATMENT Aris Junaidi
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8116

Abstract

Using light and electrone microscopic techniques the structure of the testicle, epididymis and prostate gland of mature male dogs were studied 3 months after implantation with a slow release implant containing 6 mg GnRH agonist deslorelin. After 3 months of treatment, all tubules seminiferous showed atropic and aspermatogenic. Atrophy of the Leydig cells was observed in the interstitial tissue. The glandular epithelium of the prostate showed atrophy and was non secretory. At the electrone microscopic levels, Sertoli and Leydig cells showed a marked atrophy and their spermatogenic tubules were mostly lined with Sertoli cells at the basal lamina. The prostate gland showed atrophy of the nucleous and the epithelium was non secretory. The present data showed that a slow release implant containing GnRH agonist deslorelin can be used effectively to suppress fertility in male dogs.
PREPARATION OF LYMPHOCYTE CULTURE CELL FROM PERIPHERIAL BLOOD OF NASOPHARYNGEAL CARCINOMA PATIENTS Aris Haryanto; Sofia Mubarika; Nastiti Wijayanti
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8239

Abstract

Lymphocyte is leukocyte component that difficult to culture in vitro. Several viruses need lymphocytes as host cell in order to proliferate and growth in this media such as Epstein-Barr virus (EBV). This virus was associated with malignant disease like nasopharyngeal carcinoma (NPC). The objectives of this research are to develop and to prepare lymphocyte cell culture as material source of DNA for molecular analysis of virus. Peripheral blood was collected from NPC patients which is histopatologically and serologically positive of EBV. Lymphocytes were separated from the other blood components using ficcol-histopaque. Lymphocytes were diluted using RPMt medium then they were cultivated into 96 microwell plate with concentration of 106 cell/ml. The medium consist of 10% FBS, RPMI, Penstrep and FK 506. Culture of lymphocytes were incubated in 5% CO2 at 37°C. The lymphocyte cultures developed and grew confluendy during the first week. Only B cells whith EBV would be well establish. After 50 cell generations, lymphocytes were transformed and immortalized to be lymphoblastoid cell fine (LCL).
EVALUATION OF LEAD LEVEL IN VARIOUS TISSUES OF PIGEONS (Columbia livia) IN YOGYAKARTA Edi Boedi Santosa; Bambang Hariono; Mudasir Mudasir
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8240

Abstract

Lead is one of major air pollutants. Lead is a dangerous poison for human and animals. Pigeon meat is one of popular food consumed by many people in Yogyakana. The aim of this research was to evaluate whether the lead concentration in the pigeon in Yogyakarta is considered safe for human consumption or not Forty birds 3-4 months old were collected from 4 locations in Yogyakarta, Group I (10 birds) were collected from people who sole fried birds around UripBumodibflTjo, C. Simanjuntak, Kaliurang, Gcjayan, Johanes streets, and North Ringroad. Group II (10 birds) were collected from the bird markets (Ngasem, Terban, Lempuyangan), group III (10 birds) were collected from the bird owners who raised the birds extensivelly (not in cages). Group IV (10 birds) were obtained from the bird owners who raised tho birds imeosivdly (in cages). All experimental animals were euthanized by chloroform then necropsied. Kidney, liver and breast muscle were collected for lead examination using atomic absorption spectrophotorneter {AAS). The results of the experiment showed that lead concentrations in the kidneys, liver and breast muscle of pigeons obtained from several locations in Yogyakarta were higher than the recommended standard value from WHO (0,1 ppm).
THE EFFICACY OF DORAMECTIN FOR TREATMENT OF Ancylostomiasis IN DOGS Ida Tjahajati
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8241

Abstract

A study was done to evaluate the efficacy of doramectin injection for Ancylostomiasis treatment in 10 dogs. All of the dogs were heavily and naturally infected with Ancylostoma Sp. the heavy infection criterion (25 egg per gram) was based on the flotation method. At the beginning of the study the dogs were injected doramection subcutaneously (200 ,wg/kg of body weight). The pathogenesis of the disease was observd through the clinical sign and feses examination during 2 weeks after treatment. Dogs were considered recovery when they have a good response to the treatment and clinically showed normal of apetite, no sign of diarrhea and no egg was found laboratorically. Results of the study showed that all of the dogs had positive response to the treatment and therefore it can be concluded that doramectin effective against Ancylostoma Sp. in dogs. 
HORMONAL RESPONSES OF LACTATING DAIRY COWS TO TREATMENT WITH A PROGESTERONE INTRAVAGINAL DEVICE WITH OR WITHOUT ESTRADIOL Prabowo P Putro
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8242

Abstract

The objective of this study was to evaluate the effect of progesterone supplementation through an intravaginal device on plasma progesterone and estradiol-17(3 profiles in lactating dairy cows. Eight lactating Friesian cows, between 3 and 5 years, in the luteal phase of a normal estrous cycle were used In the treated group A (n = 4), a CIDR intravaginal implant contains 1.9 gram progesterone, was inserted into the vagina of each cow at day 8 of the study and removed 9 days later. In the group B (n = 4), a CIDR combined with a 10 mg estradiol benzoate capsule was inserted into the vagina as in the group A Blood samples were taken daily for progesterone and estradiol-17(5 assays. Plasma concentrations of progesterone increased rapidly following the CIDR insertion, reached maximum at day 9 of insertion (6.28 ± 0.26 ng/ml and 5.62 + 0.16 ng/ml, respectively, P < 005), and then dropped abruptly after CIDR removal. Two days after the CIDR insertion, the plasma progesterone increased more rapidly in the group A than in the group B, and were significantly higher between days 11 and 17 of the study, but lower between days 20 (estrus) and 22 of the study (P < 0.05). The progesterone levels reached to basal levels within 3 days following the CIDR removal (0.64 + 0.12 ng/ml and 029 + 0.16 ng/ml, respectively, P < 0.05), when the cows were showing estrous behavior. The declines in the mean plasma concentrations of progesterone after the CIDR removal in the both treated groups were linear (R2 = 0.814 and 0 798, the rate of decline were 1.36 ng/ml/day and 1.29 ng/ml/day, respectively). Thereafter, the mean progesterone concentrations elevated steadily after the induced estrus. The plasma concentrations of estradiol-17(3 in the both treated groups also showed relatively the same pattern. They declined regularly in the luteal phase, increased slowly by the follicular phase, and reached maximum levels at estrus, 3.08 + 0.12 and 338 ±0.16 pg/ml (P < 0.05), respectively. The concentrations of estradiol-17(3 between days 6 and 9, days 20 (estrus) and 21 of the study in the group A were significantly lower than those in the group B (P < 0.05). The insertion of estradiol benzoate capsule significantly increased the plasma concentrations of estradiol-17(3. At the same time, the increase of plasma estradiol-17£ concentrations was followed by the decline of plasma progesterone levels. In conclusion, CIDR is a useful agent for continuous delivery of progesterone to enable estrus synchronization in lactating dairy cows. Its combination with estradiol benzoate resulted in a more marked decline in the plasma concentration of progesterone and more synchronous estrus following its removal than that of CIDR alone.
A DISTRIBUTION AND AN IDENTIFICATION OF Culicoides SPECIES (Diptera: Ceratopogonidae) IN BOGOR Ana Sahara; Malole Malole; Koesharto Koesharto; Sendow Sendow; Sukarsih Sukarsih
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8244

Abstract

A distribution and an identification of Culicoides species collected around the cattle farm have been investigated. The purpose of the research is to know the distribution and the species of Culicoides that might be a relevance to bluetongue virus tranmission. A number of 2117Culicoides (Diptera. Ceratopogonedae) was collected from around the castle farm in Depok dan Cibungbulang Bogor municipality. The collection was carried out by pirbright type miniatur light trap. The identification of species of Culicoides was based on the morphological character of the insect according to Wirth and Hubert. The result showed that the number of Culicoides was found in Depok region more than in Cibungbulang. There were 14 species identified from the two region. In Depok, the specieses of Culicoides were dominated by C. parahumeralis Wirth & Hubert, C. actoni Smith, C. oxystoma KiefFer and C. peregrinus Kiefier; whereas in Cibungbulang, they were dominated by C. parahumeralis, C. orientalis Macfiei, C. peregrinus and C oxystoma. The species suspected in their role of bluetongue transmission are C. actoni, C. peregrinus, C.oxystoma and C. orientalis.          
EFFECTS OF PAPAYA LEAF EXTRACT ADMINISTRATION IN CHICKENS: I. DUODENUM PATHOPHYSIOLOGICAL RESPONSE Mufti Kamaruddin; M Nur Salim
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8245

Abstract

The pathophysiological effects of papaya leaf extract administration were studied in 20 local-three month-old chickens weighing 577±69.979 g. The chickens were randomly evenly divided into 4 groups. P0 group just orally received one single dose Of 3.0 ml aquadest. The P1 P2 P3 and P4 groups were treated orally with one single dose of 1,5 ml, 2.0ml, 2.5 ml and 3.0 ml papaya leaf extract respectively. The extract was made from 400 g papaya leaves, extracted directly by simple traditional methods. Four days after treatment, the chickens were sacrificed, the duodenum were excised for macroscopic examinations, than fixed in 10% formalin prepared for microscopic examination using paraffin method and routine hematoxylin-eosin staining. The results indicated that pathological duodenum effects occurred in groups P2, P3 and P4. Macroscopically, the duodenum were hyperaemic and haemorrhagic. Microscopically the duodenum of these groups showed erosions of ephitelial mucous cells, hyperaemic and haemorrhagic propia. The P0 and Pt did not show any macroscopic and microscopic changes. It was concluded that one single dose of 3.0 ml aquadest or 1.5 ml of papaya leaf did not cause any pathophysiological effects in duodenum chicken, but a single dose of 2.0 ml, 2.5 ml or 3.0 ml did. The greater the dose the worse the effects.          
ANALYSIS OF COLFFORM BACTERIA IN THE WELL WATER AND ITS POSSIBLE BIOPATHOLOGIC EFFECTS Hastari Wuryastuti; R Wasito; Siti Chalimah; Sri Andayani; Yuni Indraswati; Leksono Lestariyadi; Prapti K; Mohammad Amien
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8246

Abstract

Coliform is a Gram-negative, rod shape bacteria Some strains, especially Escherichia coli can cause diarrhea among animals aad humans. Contamination of water whith pathogenic E. coli has been associated with this disease in animals and humans.In the present study, coliform bacteria were determined with most probable number (MPN) method cultured on lactose broth and brillian green bile broth. Results indicated that the well water samples collected from the areas of Karangmalang, Sungai Code and Deresan had 150 coliform/100 ml, 210 coliform/100 ml and 460 coliform/l00 ml, respectively. Whereas, standard number of coliform organisms in drinking water recommended by World Health Organization (WHO) is 10 coliform/100 ml and is 1 E. coli/100 ml. Therefore, based on the present study, the coliform organisms content in the well water is for above its normal level value as recommended by WHO. Use of the water for daily consumption, especially as drinking water needs to be a great concern
CIMETID1NE ENHIBITS ELIMINAT1ON OF SULPHAMETHOXAZOLE IN RABBIT {Oryctolagus Cuniculus) Ida Tjahajati
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8247

Abstract

Cimetidine was reported to inhibit elimination many other drugs. The research has been done to study the effect of cimetidine on elimination of sulphamethoxazole in rabbits. Twenty healthy male rabbits, 10 months of age were used as experimental animals. The rabbits were allotted into 4 groups of 5 each Group I as a control group were intramuscularly injected with 25 mg sulphamethoxazole. Group 11 were intramuscularly injected 25 sutphamethoxazole and pretreated with 12.5 mg cimetidine 30 minutes before injection. Group 111 were intramuscularly injected 25 mg sulphamethoxazole, pretreatod with 25 mg cimetidine 30 minutes before injection. Group IV were intramuscularly injected 25 mg sulphamethoxazole, pretreated with 50 mg cimetidine 30 minutes before injection. Blood sample were collecled at 0.25, 0 75, 1,75, .1.75, 10 and 24 hours after injection. Plasma sulphamethoxazole levels were determined using the Bratton-Marshall method Elimination kinetic parameters were analyzed statistically using anava. The result of the study showed that cimetidine significantly reduced total clearance of sulphamethoxazole <P<0.l). Based on the result it can be concluded that cimetidine inhibits elimination of sulphamethoxazole.
Distribusi dan Identifikasi Spesies-Spesies Calicoides (Deptera: Ceratopogonidae) di Kabupaten Bogor Ana Sahara; Malole . .; Koesharto .; Sendow .; Sukarsih .
Jurnal Sain Veteriner Vol 18, No 1 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.257

Abstract

Telah diteliti distribusi dan identifikasi spesies-spesies Culicoides yang ada di sekitar kandang temak sapi di Kabupaten Bogor. Penelitian ini ditujukan untuk mengetahui spesiesspesies Culicoides yang mempunyai peranan dalam penyebaran penyakit bluetongue pada ternak. Sebanyak 2117 ekor Culicoides (Diptem: Ceratopogonidae) dikumpulkan dari kandang ternak sapi di wilayah Depok dan Cibungbulang, Kabupaten Bogor dengan menggunakan perangkap serangga Pirbright-type miniatur light trap. Identifikasi spesies dilakukan berdasarkan karakter morfologi menurut Wirth dan Hubert. Hasil penelitian menunjukkan bahwa Culicoides lebih banyak ditemukan di wilayah Depok daripada di Cibungbulang. Ada empat belas spesies dari kedua lokasi tersebut yang berhasil diidentifikasi. Spesies Culicoides yang dominan di daerah Depok adalah C. parahumeralis Wirth & Hubert, C. acioni Smith, C. ozystoma Kieffer dan C peregrinus Kieffer; sedangkan spesies yang dominan di Cibungbulang adalah C. parahumeralis, C. orientalis, C. orientalis Macfiei, C. peregrinus dan C. caystoma. Spesies Culicoides yang dicurigai sebagai penyebar penyakit bluetongue adalah: C. actoni, C. oxystoma, C. peregrinus dan. C. orientalis

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