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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 14 Documents

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Search results for , issue "Vol 30, No 1 (2012): JUNI" : 14 Documents clear

Avian Influenza (H5N1) Bentuk Pernafasan Pada Entok (Cairina moschata) Sehat Di Yogyakarta Hastari Wuryastuti; Wasito Wasito
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Influenza virus type A H5N1 has become epidemic in poultry population in Indonesia and has been suspected sporadically to cross humans. All the available evidences suggest that the most common primary introduction of AI viruses into an area is by wild birds. The ducks form the reservoir of influenza type A viruses in nature and may spread AI virus from farm to farm by mainly mechanical transfer of infective feces, in which AIvirus may be present at high concentrations and may survive for considerable periods. In the present paper, we report on the immunohistochemical and molecular investigations performed on nine ducks (Cairina moschata)naturally infected by influenza virus type A. The H5N1 gene was detected by molecular analysis of the reversetranscriptase-polymerase chain reaction in the parenchyma of the lungs, but neither in the pancreas nor the intestines. Immunohistochemical streptavidin biotin assay of monoclonal antibody anti nucleoprotein viral antigen also only corresponded to virus location within the vascular endothelia of the lung only. Our findings may suggest that the ducks provide an environment transmission of AI virus not only by a fecal route, but also by either an airborne route or direct contact with contaminated respiratory secretions. Moreover, we suggest that surveillance activities influenza viruses of the avian origin are critical for characterizing AI virus in the ducksand requires a high level of preparedness.Keywords: ducks, immunohistochemical, molecular, monoclonal antibody, airborne route

The Analysis of Total Leucocyte, C-Reactive Protein and Fibrinogen Concentrations to Evaluate The Leakage of Enterectomy Result Dhirgo Adji
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Enterectomy is an operative method that has very dangerous risk. The purpose of this research was done to see the physiological view after surgery, especially in the total leucocyte, C-reactive protein (CRP) andfibrinogen concentrations. Nine female, healthy local dogs, 10 kgs of body weight were used as experimental study. The dogs were adapted for a week into individual cages and fed commercial dog-food and water adlibitum. In the day of 7, all dogs were fasted 12 hours for anaesthesia preparation. The dogs were then divided into 3 Groups of 3 each. Group I was used as control, it had normal enterectomy surgery. Group II was animals that had enterectomy surgery with one hole leakage. Group III was animals that had enterectomy surgery with two special hole leakages. Enterectomy itself was done on to jejunum with 5 cm of gut should be cut away. Anastomosis method was done using end to end method and interrupted suturing method with chromic cat gut 0/3. The result of the research showed that there is similarly increasing level of leucocyte and CRP in the second day after surgery and decreased gradually until the sixth day, but different type of changes in fibrinogen level. The increasing level of CRP has advantages, such as it can be used as eidker marker of inflammation or infection, or worse condition after enterectomy.Keywords : enterectomy, leucocyte, C-reactive protein, fibrinogen, inflammation

The Role of Transforming Growth Factor β-1 (TGF β-1) in The Development of Heart Disease Caused by Induction of High Lipid Diet Tisna Tyasasmaya
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Cardiovascular diseases are the disorder of artery carrying blood into heart muscle and characterized by thickening, stiffening and constriction of the artery. In spite of cardiovascular disease cases have been knownsince along time ago, the main factor, mechanism, pathogenesis and indicator of the early existence of these diseases are still unknown. Transforming growth factor β-1 (TGF β-1) is a growth factor produced by various cell types which are presumed to be involved in cardiovascular diseases. This research was done to find out the role of TGF β-1 in cardiovascular diseases. Twenty female Sprague Dawley rats, 150 grams of body weight were used in this research. Rats were adapted for seven days, and then divided randomly into two Groups, 10 rats for each.Group I was fed in standard diet containing 5% of saturated fat, while Group II was fed in high fat diet containing 30% of saturated fat. Rats were maintained for two months, corresponding to each diet treatment of both groups and water ad libitum. After two months of the diet, blood was collected from orbital vein and stored into uncoagulated tube for triglyceride measurement. Rats were then killed, neropsied and their heart tissues were collected into tubes containing 10% formaldehyde for histopathologic analysis and immunohistochemistry to see the expression of TGF β-1. The result of blood analysis showed that the average of triglyceride concentration of Group I was 126 mg/dl and 228.1 mg/dl of Group II. After statistically analyzed using student t-test, it showedthat there was a significant difference between Group I and II (P<0,05). Eventhough histopathologic analysis of heart tissue did not show any alteration, the TGF β-1 was expressed in all heart tissue of Group II . Based on the results above, it can be concluded that TGF β-1 is involved in the incidence of cardiovascular disease and it is possible to use TGF β-1 as a diagnosticmarker of cardiovascular disease.Keywords : heart, cardiovascular disease, orbital vein, high fat diet, TGF β-1

The Sensitivity of Sag 1 and Bag 1 Probes to Detect Toxoplasma gondii in The Free-Rearing Chicken Ida Ayu Pasti Apsari
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was aimed to determine the sensitivity of Sag1 and Bag1 Probe to detect in free-rearing chicken using dot blot hybridization method. Thirty serologically free-rearing chicken toxoplasmosis DNA were used as samples in this study. Sag1 and Bag1 probes were labeled by non-radioactive Dig-11-dUTP. The success of detection was based on the establishment of colored dot on the nylon membrane after detected with antibody-antiDig. The Sensitivity test of Sag1 and Bag1 probes in detection were conducted by making serial dilutions of the dot blot hybridization positive free-rearing chicken DNA. The results showed that 19 positive samples detected by Sag1 and Bag1 probe by dot blot hybridization method. The sensitivity of 5.87 pg / μl Bag1 probe to detect free-range chicken DNA was 0.23 ng / μl , and sensitivity of 6.72 pg / μl Sag1 Probe was 0.45 ng / μl. From the resuls above it can be concluded that the Bag1 probe was more sensitive than that of the Sag1 probe to detect Toxoplasma gondii of free-range chicken DNA.Keywords : Toxoplasma gondii; Sag1 and Bag1 Probe; Dig-11-dUTP; free-rearing chicken

The Normal of Peristalistic Waves In Vitro In The Small Intestine of Local Rabbits Amelia Hana Amelia Hana
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

local male rabbits aged 4 months, 1.0 to 1.5 kg body weight and healthy adaptation in individual cages with food and water ad libitum. All of rabbits were randomly divided into 2 groups: groups I (K-1) and II (K-2) eachof 4 head / group. All of rabbits fasted for 12 hours, and anesthetized with urethane 1.55 g/ kg bw in a 25% solution intraperitoneally. Each rabbit dissected, segments of small intestine (duodenum, jejunum, ileum) weretaken each 1.5 cm for the measured peristaltic wave. The K-1 group of rabbits were measured peristaltic wave using Physiograph and the K-2 with Kymograph. Physiograph or Kymograf operated after the specified time of 1 second and the impression of speed 10 mm/detik paper on the sensitivity of 0.1 and the sensitivity of the amplifier 50. During the calibration operation is often performed. Data obtained by the peristaltic waves (frequency and amplitude of contractions) small intestine for 5 minutes were analyzed by t test. The results of measurements of intestinal peristalsis by using Physiograph or Kymograf showed that the frequency and amplitude of peristaltic contractions of the proximal part is higher and more caudal direction tends to go down. Therefore it was concluded that the normal peristaltic waves of the small intestine to more caudal were getting less and less interm of the frequency and amplitude of peristaltic contractions.Keywords: rabbits,small intestine, peristaltic, physiograph, kymograph

Calcium and Phosphor Retention in The Panhisterectomized Rats Fed High Calcium Hartiningsih Hartiningsih
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The objectives of the research was to study the effect of panhisterectomy on calcium (Ca) and phosphor (P) retention in the female Sprague Dawley rats that were fed teri which ratio of Ca:P is 3:1 for 12 weeks. Ten female of Sprague Dawley rats, 6 weeks of age were randomly divided into two groups (control and anhisterectomized groups) of five each. At 8 weeks of age, the rats of panhisterectomized group were panhisterectomized. At 20 weeks of age, they were placed into individual metabolic cages for balance study. The remaining of feed was collected for calcium and phosphor analyses. Every morning, from days 4 to 8 of the balance study, urine andfecal samples were also collected at the same time. The research results showed that calcium and phosphor retentions were significantly reduced (P<0.05) in panhisterectomized group compared to that of the control group. Fecal calcium excretion was a significantly higher (P<0.05), whereas fecal and urinary phosphor excretions were significantly higher (P<0.05) in the panhisterectomized group compared to that of the control group. It is concluded that panhisterectomy leads to a reduction of the calcium and phosphor retention in the Sprague Dawley rats that were fed high calcium.Keywords : panhisterectomized, calcium, phosphor retention, individual metbolic cages, Sprague Dawleyrats

Identifikasi Leptin setelah Luka Irisan padaTikus yang Diberi Pakan Lemak Tinggi Devita Anggraeni; Dhirgo Adji; Sitarina Widyarini
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Leptin is a hormone produced by adipocytes and plays an important role in wound healing. The objectives of this research were to identify leptin and total leukocyte after incision on rat fed high fat diet and zinc topicalapplication. Sixteen male Sprague Dawley rats at 3 months of age were used in this study. Rats were randomly allotted into 4 groups (A,B,C and D) of 4. Rats in group A and B were fed normal diet, while rats in group C and D were fed high fat diet. After 2 months of treatment, skin incision was done at the back side of the rat. Incision wound was then closed with single interrupted suture. The wound of the rats in group A and C was treated with vaseline, while in group B and D was treated with zinc 10%. Three days after surgery, blood was collected from each rat for leptin analysis and total leukocyte. Leptin level and total leukocyte were analyzed statistically using ANOVA for factorial experiment (2x2). The results showed that leptin level was significantly higher in rats fedhigh fat diet compared to that of the rats fed normal diet (P<0.05). There was no significant different on total leukocyte. Therefore, it was concluded that leptin level was affected by diet, while total leukocyte was notaffected neither by diet nor topical application. However, total leukocyte tends to be lowered in the rat with zinc topical application.Keywords: high fat diet, leptin, zinc, total leucocyte, Sprague Dawley rats

Pengaruh Penangkaran terhadap Profil Eritrosit Lumba-lumba Hidung Botol dari Perairan Laut Jawa Guntari Titik Mulyani; Yuda Heru Fibrianto; Teguh Budipitojo
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

In the Java Sea, there are a lot of dolphins, that often snagged fishing nets. While In Indonesia, bottlenosedolphins are mammals protected water. The aims of this research was to study the erythrocytes profile inbottlenose dolphins before and after the captivity. Seven dolphins were used in this research. Blood samples weretaken out from the superficial vein of tail for examination of the number of erythrocytes, hemoglobin levels andvalue of hematokrit. Sampling was done when the dolphins appointed from the waters of the Java Sea and afterexperiencing dolphin captivity in PT. WSI, Kendal, Central Java. The data before and after captivity were compared and tested with the statistical analysis on the level of significance of the paired t-test 95%. The results showed the significant increase in the number of erythrocytes, hemoglobin levels and the values of hematokrit significantly. It was concluded that captivity done by WSI Corporation, Kendal, Central Java did increase the profile of erythrocytes in bottlenose dolphins.Keyword : Bottlenose dolphins, captivity, profile erythrocytes, superficial vein of tail, paired t-test

Studi Lesi Makroskopis dan Mikroskopis Embrio Ayam yang Diinfeksi Virus Newcastle Disease Isolat Lapang yang Virulen Hamdu Hamjaya Putra; Haryadi Wibowo; Tri Untari; Kurniasih Kurniasih
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Newcastle disease (ND) is caused by Avian paramyxovirus, family Paramyxoviridae, one of the major diseases in chickens. This research was aimed to find lesions in chicken's embryo organs macroscopically andmicroscopically, infected by pathogenic ND virus. Embryonic chicken eggs (ECE) were inoculated by the ND Salatiga virus and ND La Sota virus as a control avirulent virus. Aquabidestilata used as a negative control. ECEwich showed the death of the embryos, stored in the refrigerator. The allantois fluid was collected, for further examination of viral growth. Chicken embryos that died then observed macroscopically. The organs of chicken embryos were made into histopathologic preparations stained with Hematoxylin and Eosin (H&E) for microscopic analysis. The identification of ND virus growth on isolates was done by haemagglutination andhaemagglutination inhibition test using an anti-ND serum. The chicken embryos that were infected by the ND Salatiga virus died approximately 26 hours post-inoculation. Macroscopic lesions were visible as haemorrhagein the skin. Microscopic lesions indicated the congestion and haemorrhage in lungs, inflammation and congestion in the skin, congestion in intestines, liver, kidneys and heart. There was also mild congestion on theskin in chicken embryos infected by ND La Sota virus. The microscopic lesions showed congestion in lungs, liver, kidneys and heart, also the inflammation and congestion on the skin. The macroscopic and microscopic lesions of chicken embryos infected by the ND Salatiga virus were more severe than lesions caused by ND La Sota virus.Key words: Newcastle disease, chicken embryos, macroscopic lesions, microscopic lesions, La Sota

Histopathologic Feature(s) of The Dactylogyrus sp-Infected Gills in The Ornamental Marine Fish Putu Eka Sudaryatma; Ni Nyoman Eriawati
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

In the present study, Dactylogyrus sp. was examined in the gill(s) of the ornamental marine fish due to the fact that this kind of parasite is very often found in those fishes without any pathognomonic clinical signs orpathologic anantomic lesions. The gill(s) samples collection and examination were performed in the Laboratory of Balai Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan. during Janury-July 2011. At first, the gills were examined natively and were subsequently processed for histopathologic examination. In the native examination, it was found the Dactylogyrus sp. with their size(s) ranging from 200-500 um. At necropsy, the gills were swollen and looked darker than normal. Based on the histopathologic lesions, the epithelial cells of the gills lamellas either primary or secondary became hyperplasia and fusion. The fusion of the gills is primarily thought to cause fatal to the fish due to significant oxygen depletion.Keywords: ornamental marine fish, gill, Dactylogyrus sp., native examination, hiperplasia and fusion.

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