Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

Jurnal Sain Veteriner

jsv
Website

Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 14 Documents

1 2

Search results for , issue "Vol 31, No 2 (2013): DESEMBER" : 14 Documents clear

Ovulatory Follicular Dynamics After Estrus Synchronization Using Intravaginal Progesterone Implant in Dairy Cows Prabowo Purwono Putro
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract The study aimed to follow ovulatory follicular dynamics and plasma progesterone profile after estrus synchronization using progesterone intravaginal implant CIDR and its combination with PGF2a and GnRH. A total of 15 non-pregnant dairy cows, 4-5 years of age, healthy and reproductively sound were divided into 3 treatment groups. Treatment 1, CIDR implant was inserted intravaginally for 9 days (CIDR); treatment 2, given CIDR and 250 mg GnRH intramuscularly at implant removal (CIDR + GnRH); treatment 3, CIDR and 25 mg PGF2a at day 7 of implant insertion (CIDR + PGF2a). Transrectal ultrasonographic examination using real time, B-mode, with 7.5 MHz transducer was performed everyday for 12 days to follow ovulatory follicular dynamics. Blood plasma was taken every day for progesterone determination using EIA technique. Data were tested using analysis of variance and correlation analysis. The result indicated that CIDR implant was a potent agent for estrus synchronization with the onset of estrus was 66.18 + 03.42 hours after its removal. The addition of GnRH resulted the highest ovulatory growth rate following the implant removal (1.67 + 0.17a, 1.93 + 0.13b dan 1.53 + 0.20a mm/day, P < 0.05). Insertion of CIDR hampered dominant follicular development and its removal caused rapid follicular growth and followed by ovulation. Its insertion resulted steady increase in plasma progesterone levels and its removal resulted in sudden decrease in the hormonal levels, induced ovulatory follicular development, followed by estrus and ovulation. The CIDR implant also inhibited luteal growth, caused no effect of PGF2a administration on decrease of plasma progesterone levels. Addition of GnRH at the time of CIDR removal enhanced the highest rate of ovulatory follicular growth.

Isolation, Identification and Sensitivity Test of Staphylococcus aureus against Amoxicillin of the Milk sample in the Mastitis Crossbred Ettawa Goat at Girimulyo Area, Kulon Progo, Yogyakarta Amalia Krishna Dewi
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus is the main bacteria causing mastitis of the Ettawa crossbreed goat. The mastitis causes significant economic losses due to decreasing of milk production. Mastitis in goat could threat the survival rate of her kids because decrease in quality and quantity of milk products. The toxin produced by S.aureus can also cause the death of their mothers. The present research is aimed to isolate and identify S. aureus from mastitis milk sample of Ettawa crossbreed goat, and does its sensitivity to amoxicillin as well. The milk samples were collected from the mastitis crossbreed Ettawa goat at Girimulyo area, Kulonprogo, Yogyakarta. In the present study, 20 milk samples of Ettawa crossbreed goat were used to isolate and identify S. aureus based on the culture, Gram staining, biochemical and sugar tests. The isolates then tested for their sensitivity to amoxicillin. Nine isolates in the present study identified as S. aureus in which they grew on MSA, formed round cells cluster, Gram +, fermented manitol, lactose and maltose. They coagulated the rabbit plasma and reacted positively to the clumping factor and Voges Proskouer test. Based on the results of the sensitivity test to 10 ìg amoxicillin is known that 8 isolates (88,89%) are sensitive to amoxicillin. Therefore, it can be concluded that there are 9 isolates from 20 mastitis milk samples of Ettawa crossbreed goat identified as S. aureus. Staphylococcus aureus isolates are mostly sensitive to amoxicillin.

Isolasi and Identification of Bacteria from the Urine Fluid Organic Fertilizer (POC)Ettawa Crossbred (PE) in The Sleman Regency Widodo Suwito; A.E.T.H. Wahyuni; Widagdo Sri Nugroho; Bambang Sumiarto; Utomo Bimo Bekti
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Urine is a by product from Ettawa crossbred (PE) goat farm in Sleman regency. In addition, the manure urine goats can be used for fluid organic fertilizer (POC). Recently, organic farming is more interesting for people because it does not use any chemical fertilizers. Salmonella sp and E. coli O157:H7 are pathogenic bacteria that can contaminate agricultural products and dangerous for public health. One of sources of contamination in agricultural products is due to the organic fertilizer. Therefore, the aim of the present study was to determine pathogenic bacteria from POC urine PE goats in Sleman regency. A total of 8 POC samples were collected from PE goat urine in Sleman regency. These samples were analyzed for Salmonella sp, E. coli O157:H7 and other pathogenic bacteria by using biochemical reactions. All samples are negative for Salmonella sp. and E. coli O157:H7 as well. Whereas, 85% of 8 samples are positive for Pseudomonas sp. The present study showed that POC from PE goats farm in Sleman regency are negative for pathogenic bacteria.

Influence of Palm Sugar Water in the Native Chicken Performance Fera Aryanti; Muhammad Bayu Aji; Nugroho Budiono
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Palm sugar containing 66,18% sukrose is an additional source of energi quickly available to the chicken. A study to examine the effect of palm sugar in the native chicken performance was held in animal health training center, Cinagara-Bogor lasted from August until November 2012. This present experiment using 1274 native chicken that were kept starting DOC. Palm sugar concentrations given in the drinking water as much as one percent started to be given to the chcken when they were still DOC. After that, the palm sugar water was given continously intermittent interval by administering multivitamin. The control group was not given the palm sugar. Chicken aged 0 to 6 weeks were given palm sugar twice a day. After reaching over 6 weeks old, palm sugar water was only given onece a day. Result obtained showed that the administration of palm sugar water affected the increase in body weight gain, feed consumption and lower mortality as well. The result also showed that the FCR is lower than that of the control group until 6 weeks old.

A Life Table of Spodoptera litura Fabr. with Different Artificial Diets Sri Lestari; Trisnowati Budi Ambarningrum; Hery Pratiknyo
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Sustainable insect supply is a routine activity in research of pest control, experimental insecticide bioassay, entomophatogen, parasitoid, and nature of competitor. Armyworm (Spodoptera litura Fabr.) is one of the alternative test insect. A mass rearing method is required to provide high demands of S. litura. A life table of insect is regularly used to study several biological parameters including the net reproduction rate of population (Ro), the mean length of life period in one generation (T), reproduction potential value of one generation from a population (rm), and the potential of a population to multiply in one generation (ë). The aim of this study were to asses the development time and reproduction potential of S. litura which different of artificial diets. Results showed that S. litura on artificial diet in recipe of Singh and Moore is T = 29,90, R = 2692,33, r = 0,26, ë = 1,29, o m whereas artificial diet in recipe of Waldbauer et al. is 32,29, R = 891,99, r = 0,21, ë = 1,23. o m

Protection Capacity of Kaffir Lime (Citrus hystrix) Peel Extract Against Dengue Haemorrhagic Fever Mosquitoes Joni Hendri
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract The use of chemicals as mosquitos repellent may cause health problems. Extract of caffir lime (Citrus hystrix) as mosquitoes can be used as another option.The present study was aimed to analyze the protection capacity of C. hystrix against Aedes aegypti and Ae. albopictus. The experimental study using completely randomized design was done. The obtained data were calculated using the protection capacity formula and analyzed using t-test. The result indicated that protection capacity of Citrus hystrix extract for 6 hours on average gave 34.82% of protection against Ae. aegypti and 41,44% of Ae. albopictus.The caffir lime extract has been able to reject the mosquitoes, Ae. aegypti and Ae. albopictus. Although the thrust of the caffir lime is not as good as chemical products, butit can be used as alternative mosquitoes repellent.

Identification of Campylobacter jejuni Using Polymerase Chain Reaction Method Liza Angeliya; Ruri Rumpaka Kurdiwa
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Campylobacter jejuni is a curved-rod shaped bacteria, non-spore, microaerophilic Gram-negative and motile. It is commonly found in the feces of animals. They grow at a temperature of 37-42° C and are zoonotic causing a disease called campylobacteriosis. Campylobacter jejuni naturally presents in the chicken digestive tract and does not cause any diseases. However, chicken carcasses contaminated with this bacteria are closely related to the the incidence of campylobacteriosis in humans. Cases of human campylobacteriosis primarily caused by Campylobacter jejuni contamination on chicken carcasses. During the cutting process, Campylobacter jejuni will be able to spread to the chicken carcasses. Alternative approach for detecting Campylobacter jejuni contamination of chicken products is by using polymerase chain reaction (PCR) method. Therefore, to determine diagnosis of animal diseases in relation to the veterinary public health due to Campylobacter jejuni is by applying PCR with forward primer: 5'-TGACGCTAGTGTTGTAGGAG-3 and reverse primer: 5'-CCATCATCGCTAAGTGCAAC-3'. The PCR primers can amplify DNA Campylobacter jejuni as long as 402 bp. The PCR method is a molecular laboratory approach which is sensitive, specific, rapid and accurate for determining diagnosis of campylobacteriosis.

The Detection of Encoding Gene of Toxic Shock Syndrome Toxin-1 S. aureus Isolate from the Milk of Cows and Goats by Polymerase Chain Reaction Rony M Kunda; A.E.T.H. Wahyuni; Rini Widayanti
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Staphylococcus aureus is the main bacterium found in cow's and goat's milk. The bacteria can produce toxin called toxic shock syndrome toxin-1 (TSST-1) that can infect humans and animals causing several serious diseases. The objective of this study was to detect the existence of encoding gene of TSST-1 S. aureus isolate from cow's and goat's milk. The research is initiated by re-identification stage of S. aureus conventionally continued by identification based on the molecular method of polymerase chain reaction (PCR). A number of 10 S. aureus isolates from cow's and goat's milk cultured in aerobics continued by Gram stain, catalase, coagulase, MSA, VJA and VP tests. The identification of S. aureus based on the molecular approach conducted by 16S rRNA gene amplification continued with amplification of TSST-1 encoding gene as the target gene. The PCR product of TSST-1 encoding gene then sequenced to ensure whether the DNA fragment amplified is the TSST-1 encoding gene or not. The result of the research indicates that re-identification of S. aureus conventionally generating positive reaction of S. aureus species. Molecular identification of 16S rRNA gene amplification gives a good result by producing DNA fragment of 745 bp size and meets the target gene. The detection result of encoding gene TSST-1 gives negative result marked by DNA fragment which the size does not match the target gene. The allignment result of sequence isolate SA.1 indicates that the sequence is not tst gene but the gene which coded glutamate sinthetase belongs to S. aureus, whereas sequence of isolate KI.8 is 50S rRNA gene belongs toS. saprophyticus.

Blood Profile of Wistar Rats due to Subcronic Condition Caused by Sodium Nitrite Dyah Ayu Widyastuti
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Nowadays, chemicals used as food additives are increasing. One of the chemicals commonly used is sodium nitrite (NaNO2 ). Sodium nitrite is found in sausage and another meat product. Sodium nitrite is used as food preservative agent. But, the presence of NaNO2 can influence blood cells ability to carry oxygen. It causes anemia and forms nitrosamines, carcinogenic agents. This research is purposed to know blood profiles of Wistar rats on subchronic condition caused by NaNO2 . The research was done for 3 months as subchronic time. Twenty seven Wistar rats were divided into 3 groups, control group (K), first group (P1) treated by NaNO2 with dose of 11,25 mg/kg bodyweight/day, and second group (P2) treated by NaNO2 with dose of 22,50 mg/kg bodyweight/day. Blood samples were collected from orbital sinus and tested once a week for hematological parameters, including white blood cells (WBC), red blood cells (RBC), hemoglobin (Hb), hematocrit (HCT), lymphocyte level, and neutrophil level. The data were analyzed with ANOVA using SPSS 16 program for Windows and the blood profiles alteration were seen. The results from this study showed there were no significant differences among the blood profiles of Wistar rats treated by NaNO2 with dose of 11,25 mg/kg 2 bodyweight/day and dose of 22,50 mg/kg bodyweight/day compared to that of the control group, except WBC values at subchronic condition at dose of 22,50 mg/kg bodyweight/day which decreased 23,46%. The NaNO 2 could not influence the blood profiles of rats treated by NaNO2 subchronically.

The Effects of Turmeric Extract (Curcuma domestica Val) on Cigarette Smoke Cardiotoxicity in The Wistar Rats Nida Ul Millah; Agapietta Kusumawardani; Laelatul Rahmad; Novi Herliyani; Kurniati Sarwendah; Bambang Sutrisno; Hastari Wuryastuty; R Wasito
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.3811

Abstract The objective of this research is to determine the effects of turmeric (Curcuma domestica Val) extract administration toward heart histopathologic lesions of male albino Wistar rats + 3 months old exposed by cigarette smoke. Twenty male Wistar rats were divided randomly into four groups of five each: Group I was given neither cigarette smoke exposure nor turmeric extract administration (control) (KI), Group II was given cigarette smoke exposure of 9 cigarettes/ day (KII), Group III was given 1 ml turmeric extract 0.25% (KIII) and Group IV was given cigarette smoke exposure of 9 cigarettes/ day and 1 ml turmeric extract 0.25% (KIV). After 2 months of treatment, necropsy was conducted to all rats and the heart samples were collected to be processed histopatologically and stained routinely with hematoxylin-eosin. The results of the present study indicated that the cigarette smoke caused cardiomyopathy in KII marked by the histopathologic lesions such as vacuolation, homogeneous eosinophilic cytoplasms, edema and necrosis of myocardia, also congestion and mild hemorrhages (KII). Whereas, rats in Groups KI, KIII and KIV had normal histologic structures of myocardia. It is concluded, that turmeric extract could be a preventive herbs toward cardiotoxic effects of cigarette smoke. Further research has to be conducted to determine the anti cardiomyopathy mechanism of turmeric rhizome due to cigarette smoke exposure.

Page 1 of 2 | Total Record : 14

1 2

Filter by Year

2013 2013

Filter By Issues

All Issue Vol 43, No 3 (2025): Desember Vol 43, No 2 (2025): Agustus Vol 43, No 1 (2025): April Vol 42, No 3 (2024): Desember Vol 42, No 2 (2024): Agustus Vol 42, No 1 (2024): April Vol 41, No 3 (2023): Desember Vol 41, No 2 (2023): Agustus Vol 41, No 1 (2023): April Vol 40, No 3 (2022): Desember Vol 40, No 2 (2022): Agustus Vol 40, No 1 (2022): April Vol 39, No 3 (2021): Desember Vol 39, No 2 (2021): Agustus Vol 39, No 1 (2021): April Vol 38, No 3 (2020): Desember Vol 38, No 2 (2020): Agustus Vol 38, No 1 (2020): April Vol 37, No 2 (2019): Desember Vol 37, No 1 (2019): Juni Vol 36, No 2 (2018): Desember Vol 36, No 1 (2018): Juni Vol 35, No 2 (2017): Desember Vol 35, No 1 (2017): Juni Vol 34, No 2 (2016): Desember Vol 34, No 1 (2016): Juni Vol 33, No 2 (2015): Desember Vol 33, No 1 (2015): JUNI Vol 32, No 2 (2014): DESEMBER Vol 32, No 1 (2014): JUNI Vol 31, No 2 (2013): DESEMBER Vol 31, No 1 (2013): JULI Vol 30, No 2 (2012): DESEMBER Vol 30, No 1 (2012): JUNI Vol 29, No 2 (2011): DESEMBER Vol 29, No 1 (2011): JUNI Vol 28, No 2 (2010): DESEMBER Vol 28, No 1 (2010): JUNI Vol 27, No 2 (2009): DESEMBER Vol 27, No 1 (2009): JUNI Vol 26, No 2 (2008): DESEMBER Vol 26, No 1 (2008): JUNI Vol 25, No 2 (2007): DESEMBER Vol 25, No 1 (2007): JUNI Vol 24, No 2 (2006): DESEMBER Vol 24, No 1 (2006): JUNI Vol 23, No 2 (2005): DESEMBER Vol 23, No 1 (2005): JUNI Vol 22, No 2 (2004): DESEMBER Vol 22, No 1 (2004): Juli Vol 21, No 2 (2003): DESEMBER Vol 21, No 1 (2003): JULI Vol 20, No 2 (2002): Desember Vol 20, No 1 (2002): Juli Vol 19, No 2 (2001): DESEMBER Vol 18, No 1&2 (2000) Vol 18, No 2 (2000) Vol 18, No 1 (2000) Vol 17, No 1 (1999) Vol 16, No 2 (1999) Vol 16, No 1 (1998) Vol 15, No 1&2 (1996) Vol 14, No 2 (1995) More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kab. sleman, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA