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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 14 Documents

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Search results for , issue "Vol 32, No 2 (2014): DESEMBER" : 14 Documents clear

The Accuracy of Transrectal Ultrasonography Diagnostic for Examination of Ovarian Structures in Cattle Prabowo Purwono Putro
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The study aimed to determine the accuracy of ovarian follicle and corpus luteum measurements in vivo using ultrasonography diagnostic and in vitro after slicing of the ovaries. A total of 15 cows were examined using transrectal ultrasonography just before being slaughtered. Ultrasonographic examination was performed using 7.5 MHz transrectal tranducer on ovaries, measured dominant follicle and corpus luteum diameters. Follicleappeared echogenic, black with round shape. Corpus luteum, appeared as low echogenicity structure and grayish in color.Ovaries were taken out soon after animals slaughtered, soaked in 10% buffered formalin for 12 hours,then cut in thin slices and measured follicle and corpus luteum diameters. Results of follicle and corpus luteum diameter measurements in vitro and in vivo compared using two sample or paired t-test, correlation and regression analyses. The two methods of follicle and corpus luteum measurements had linear orrelation (follicle measurement, R2 = 0.933 , r = 0.966, n = 15, P < 0.05) (corpus luteum, R2 = 0.912, r = 0.955, n = 15, P < 0.05).Measurements of dominant follicle diameter and corpus luteum size manifested the real size of the two ovarian structures. Transrectal ultrasonography diagnostic on ovaries proved to be having high accuracy and reliability for identification, measurement of ovarian structure diameters, follicle and corpus luteum in cattle.

A Study of the Fruit Bat (Rousettus sp) Brain Anatomy as Natural Reservoir Wild Animal for the Rabies Virus Karina Mayang Sari; Siti Khomariyah; Nur Arofah; Sabrina Wahyu Wardhani; Tri Wahyu Pangestiningsih
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Rousettus sp. (Fruit bat) is one type of fruit bats in Indonesia and act as a natural reservoir of rabies. Rabies is caused by a virus from genus Lyssavirus, family Rhabdoviridae, which attack central nervous system (CNS).The brain is an organ that is sensitive to rabies infection. The purpose of this study was to determine the anatomical structure of the fruit bat brain macroscopically. Five fruit bat were used in this study, they were anaesthetized using ketamine and xylazin. Animals were perfused using physiological saline and 10% buffered formalin. Brains were taken using tweezers after all the bones of the skull were separated. Analysis of macroscopic brain was done descriptively. The results showed that the fruit bat brain were generally divided into cerebrum, cerebellum and brain stem. Gyrus, sulcus and the paraflokulus lobes of the fruit bat brain were less developed than that of the dogs brain.

Cholesterol Levels In Bligon Goats During Estrous Cycle Nadia Kartikasari; Risa Ummami
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The study was aimed to assess the status of cholesterol levels during the estrous cycle in goats. The present study was conducted by using 4 female cycling bligon goats (Capra hircus), aged 2-3 years, weighed 27-45 kgs, and clinically healthy and Venous blood sampling was done on days 1 (estrus), 3-4 (early luteal), 10-16 (late luteal), 17-20 (proestrus) using a syringe and collected in tubes containing lithium heparin. Plasma wasseparated by centrifugation, put into 1.5 ml Eppendorf tube and stored at a temperature of -20° C until analysis performed. Cholesterol levels were measured by using spectrophotometric method. Cholesterol concentrations during the estrous cycle were analyzed by General Linear Model Repeated Measure. The average of cholesterollevels in bligon goats is 52.20 ± 11.11 – 64.50 ± 18.46 mg/dl. The results showed that the concentration of cholesterol was between phases during the estrous cycle was not significantly different (P>0.05). However, there was a decreasing tendency of cholesterol concentration at the early luteal phase (52.20 mg/dl) and at the late luteal (53.19 mg/dl) compared to the levels at the follicular phase (65.50 mg / dl at estrus and 59.22 mg / dl at proestrus ). It can be concluded that the plasma level of cholesterol does not change significantly during theestrous cycle.

Prevalency of Nematode in Bali Cattle at Manokwari Regency Muhammad Junaidi; Priyo Sambodo; Dwi Nurhayati
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was conducted from August to October 2006, a disease caused by worms digestive tract is one of the causes of the decline in beef production . The purpose of this study was to assess the number of infected cattle nematode, the nematode types and amount of each type of nematodes in Bali cattle in Manokwari. Based onobservations on the worm eggs, total of 23 individuals (44.23 %) of Bali cattle ( puppies and adults ) infected with nematodes. Most of the infected cow is a cow that as many as 16 puppies cattle (69.56 %), while the remaining 7 animals (30.44 %) is the mother cow. Types of nematodes that infect cattle puppies and adult cattle are Cooperiasp., Bunostomum sp., Mecistocirrus sp. and Trichuris sp. The frequency of attacks in a row is as much as 13 cattle Cooperia sp, Mecistocirrus sp as many as 10 head of cattle, as many as 8 sp bunostomum cows and Trichuris sp. as much as 5 cows. The highest number of eggs in a row is Bunostomum sp. (1520), Cooperia sp. (1280), Mecistocirrus sp. ( 1200) and Trichuris sp. (280). The average number of eggs per gr feces ( EPG ) of 4 species of parasites that infect a row is Bunostomum sp . (190 EPG), Mecistocirrus sp. (120 EPG) Cooperia sp. (98.46 EPG)and Trichuris sp. (56 EPG). As many as 44.23 % of Bali cattle in three districts (Masni, Oransbari dan Prafi) attacked nematodes which are predominantly cattle aged less than one year .

Stability of gag-ca gene of Jembrana Virus in pcDNA-ca recombinant plasmid vector in Escherichia coli DH5α which has been stored for eight years Baso Yusuf; Adyatma M Nur; Nur Fiska Yunitasari; Tri Ari Widiastuti; Endah Puspitasari
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aim of this study was to determine the stability of gag-ca gene in a pcDNA-ca recombinant plasmid vector in Escherichia coli DH5α which has been stored for eight years using Polymerase Chain Reaction method (PCR). Gag-ca gene which codes mayor protein has been characterized as immunodominan antigen, and it also has positive reaction to animal antibody which infected by Jembrana virus, so it usually used as vaccine resources and serologic detection for Jembrana disease. The research was done by analyzing gag-ca gene of Jembrana virus in pcDNA-ca recombinant plasmid in Escherichia coli DH5α which has been stored for eightyears. The pcDNA-ca recombinant plasmid was purified from cultured Escherichia coli DH5α recombinant bacteria using "High Pure Plasmid Isolation Kit", then amplificated with “Pure Taq-Ready To Go PCR Kit” using specific primers. PCR products showed a positive result that pcDNA-ca recombinant plasmid contains gag-ca gene. PCR products identification using electrophoresis on agarose gel 1% showed that DNA stand apparently carried gag-ca gene at approximate amplicon 210 base pair (bp).

Comparison of Toxoplasma gondii Cysts Predilection in the Heart and Diaphragm of Pig in Bali I Wayan Wirata
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

A study was conducted on Toxoplasma gondii in the heart and diaphragm of pigs in Bali. The study was aimed to compare the predilection of Toxoplasma gondii cysts in the hearts and diaphragms of pigs and compared to the number of T. gondii cysts per gr from the hearts and diaphragm sof infected pigs. Pigs were examined as many as 171. Each organ was examined by digestion method in order to identify the presence of T. gondii cysts in these organs. The parameters measured were the percentages and numbers of T. gondii cysts which werefound in each organs. The results showed that 9 of pigs were infected with T. gondii (5,26%) which in the hearts and diaphragms were 4.09% and 1.17%, respectively. The average number of T. gondii cysts per gr of the heart and diaphragm were 30.86 ± 21.38 and 24, respectively.

Chemistry and Microbiology Qualities of Hard Candy-Green-Sirih Leaves (Piper betle L.) As Cattle Feed Additive Anika Prastyowati; Lorensia Maria Ekawati Purwijantiningsih; Fransiskus Sinung Pranata
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Cattle diseases caused by bacterial infection and unqualified feed can decrease production of beef which people consume more in Indonesia. This research objective is to know quality of hard candy as cattle feed additive including chemistry and microbiology characteristics. Research steps consist of material preparation test, making sirih leaf extract, making hard candy, chemistry tests (The levels of water, ashes, reduction sugar, sacarose and atsiri oil, respectively) and microbiology test (Total count test, and yeast and Fungi Test). Theresults showed that hard candy made fulfill SNI (Indonesia National Standar) considered of water level, ashes level, sacarose level, total count, and yeast fungi level. Atsiri oil level before sirih leaves are made into hard candy is 0,405% and it still exists in hard candy with increasing atsiri oil level, 0,1333%.

Production Of Crude Aspergillus Fermentation Extract To Enhance The Quality Of Feed As A Productivity Booster Of Super Chicken Aris Munandar; Vembriarto Jati Pramono
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Chicken meat is one of the most popular products that is really interesting for the consumers.The aim of this study was to produce feed supplement that can be used to improve food digestibility and productivity as well in local super chicken. In this research, Group I (control group) was given feed and drink. ad libitum , Group II was given feed and drink ad libitum with the addition of fermented Aspergillus niger a dose of 4 ml / L of drinkingwater. Group III was given feed and drink ad libitum with the addition of fermented Aspergillus oryzae a dose of 4 ml / L of drinking water for 60 days, and then weight gain, food conversion rate as well as calcium and phosphate levels in the blood were observed. The results were analyzed using analysis of variance and showed that there were weight gain differences between the control and treatment groups of A. niger. There is no weight gain difference between the control and feed supplement groups . The blood calcium and phosphate levelsbetween the control and feed supplement groups were also not different.

The Effect of Sinbiotic Supplement as Replacement for Antibiotic Growth Promoter on Growth and Size of Intestinal Villi in Broiler Chicken Muhammad Arifin; Vembriarto Jati Pramono
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Over the past few decades, antibiotics have been used as growth promoters in poultry diet to improve animal performance and to obtain economic benefits. The aim of this study was to know the effect of sinbiotic derived from Bacillus subtilis as probiotic and Saccharomyces cerevisiae cell wall combination as one of antibiotic growth promoters (AGP) in broilers. Group 1 (control), group 2 (AGP), and group 3 (sinbiotic). Broilers from each group were necropsied for histological preparation and the length and width of intestinal villus were measured histologically. The data were taken from feed, body weight, Feed Conversion Ratio (FCR), and the length and width of intestinal villi. Analysis of Variance were used to analize the data. The results of the length and width of the duodenum, jejunum, and colon villi in group 3 and group 1 were showed significant differences (P <0.05) as well as a comparison between group 3 and group 2. Statistical analysis of weight gain during 5 weeks was showed significant differences (P <0.05) between groups except at week 2 and 3. Analysis of FCR was showed that there were significant differences (P <0.05) between groups on a weekly basis. It was concluded that sinbiotic increase the length and width of intestinal villi, increase the body weight gain ofchicken, and reduce the value of FCR, therefore sinbiotic can be used as an alternative replacement for Antibiotic Growth Promoter (AGP).

Effect Of Keren Fruit Extract (Muntingia calabura) On Blood Glucose Levels Of Rats (Rattus novergicus) Which Induced By Streptozotocin (STZ) Vembriarto Jati Pramono; Rahmad Santosa
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Diabetes mellitus is reveal various symptoms that characterized by increasies of blood glucose levels (hyperglycemia) due to insulin deficiency. Kersen fruit (Muntingia calabura) is thought to contain antidiabetic substance such as ascorbic acid, fiber, beta-carotene, riboflavin, thiamine and niacin. The study was conducted to determine the potential of kersen fruit in decreasies blood sugar levels. The group divided into group I (negativecontrol), group II (positive control), group III, IV, and V were given kersen fruit extract 100 mg/kg BW, 200 mg/kg BW, and 400 mg/kg BW respectively. Rats of groups I-V were induced with streptozotocin (STZ). Blood sugar values were analyzed using Analysis of Variance Repeated method (Repated ANOVA). The results showed blood glucose levels before treatment, week-0, and week-2 in the group I (133 mg/dL, 164 mg/dL, 105 mg/dL), group II (136 mg/dL, 362 mg/dL, 431 mg/dL), group III (133 mg/dL, 513 mg/dL, 109 mg/dL), group IV (100 mg/dL, 376 mg/dL, 153 mg/dL), and group V (83 mg/dL, 225 mg/dL, 169 mg/dL). Respectively based on statistical analysis showed a significant decreasies of blood sugar levels of the group III so that kersen extract with the dose of 100 mg / kg has the potential to antidiabetic.

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