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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 16 Documents
 1   2 
Search results for , issue "Vol 36, No 2 (2018): Desember" : 16 Documents clear
Daya Anthelmintik Infusa Biji Buah Pinang (Areca catechu) Terhadap Cacing Ascaridia galli Secara in Vitro Wida Wahidah Mubarokah; Wisnu Nurcahyo; Kurniasih Kurniasih
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7800.591 KB) | DOI: 10.22146/jsv.40337

Abstract

A. galli infection caused high economic losses and decreased the amount and quality of egg production. The existence of anthelmintic resistance lead to the development of research on alternative treatments for A. galli. This aim of the research was to know LC50 of Areca catechu on infestation of A. galli. In this research A. galli was taken from a chicken slaughterhouse in Terban, Yogyakarta, and was divided into 8 groups for in vitro research. Group I was treated with an 10% infusion of A. catechu; group II was treated with 12.5% infusion of A. catechu; group III was treated with a 15% infusion of A. catechu; group IV was treated with a 17.5% infusion of A. catechu; group V was treated with 20% infusion of A. catechu; group VI was treated with a 22.5% infusion of A. catechu; group VII was treated with 25% A. catechu infusion and group VIII as negative control (0.9% NaCl). A. galli mortality is recorded every one hour until worm mortality is 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test the infusion of Areca catechu against Ascaridia galli can cause death in worms with an LC 50 calculation of 21. 18%.
Kombinasi One-step Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) dan Nucleic Acid Lateral Flow (NALF) sebagai Metode Deteksi Gen env-tm Virus Jembrana Strain Tabanan 1987 Asmarani Kusumawati; Fatimah Fatimah
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8202.514 KB) | DOI: 10.22146/jsv.41146

Abstract

Jembrana disease is an acute viral disease  in Bali cattle that have short incubation period, high mortality and morbidity rate. Early diagnostic methods are needed  to prevent the further spreading of this disease. In this study, we combined One-step RT-PCR  and  NALF  methods to detect env-tm gene of Jembrana virus Tabanan 1987 strain. Viral RNA isolated from a spleen of the infected cattle was used as the template. One-step RT-PCR procedure was performed using oligoprobes  labeled digoxigenin and  right primer sequence that were designed using the primer3plus program based  on the conserves region of env-tm gene from NCBI database. The product of one-step RT-PCR was tested using NALF  method  instead of  electrophoresis. Positive result was shown by the appearance of dark lines on the test line of digoxigenin in NALF dipstick device.
Body Weight, Physiological Status and Volatile Fatty Acid on Kacang and Etawah Crossbreed Goat by Reduction and Refeeding of Feed Quantity Bambang Suwignyo; Panjono Panjono; Aryanto Aryanto; Sarmin Sarmin; Irkham Widiyono
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (11278.81 KB) | DOI: 10.22146/jsv.41149

Abstract

The aimed of this study was to observe the effect of feed restriction and refeeding on body weight, physiological status and blood VFA on Kacang and Etawah Crossbreed Goat. Eight male goats consisting of 4 Kacang Goats and 4 Etawah Crossbred goats were used as experiment objects in this study. Both types of goats were subjected to the same treatment (three phases). First was adaptation, feed was given gradualy until reach the intended amount. Secondly, the feed was reduced by giving as much as 1.7% dry matter of body weight for each; thirdly, both goats were fed on ad libitum (refeeding phase). The variables observed were body weight changes, physiological status and blood VFA. The data obtained were analyzed by Various Random Test using Completely Randomized Design (RAL) on 2x2 factorial patterns. Duncan Multiple Range Test was used to test treatments that significantly different. The results showed that the pattern of changes in body weight on both goats breeds were relatively similar. Physiological status (pulse, rectal and respiratory temperature) on both breed of goats were not significantly different. However, decrease in the amount of feed impacted in the decrease in body temperature, pulsus and respiration, although it was still within normal range. The same also happened on blood VFA levels. Based on the results of the study, it can be concluded that breed differences breed of goat did not affect the physiological condition of goat. Differences in the amount of feed affect the physiological conditions and the performance of goat production.
Efektifitas perlakuan awal Tretinoin 0,1% Topikal pada Proses Penyembuhan di Luka Insisi pada Mencit (Mus musculus) Iswinarno Doso Saputro; Hesty Aulia Palupi; Wiwik Misaco Yuniarti
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9452.348 KB) | DOI: 10.22146/jsv.41152

Abstract

Tretinoin has an important role in wound healing process include improve fibroplasia and collagen synthesis, maintening humoral immunity, and neutralize steroid effect.Epithelization  and fibroblast proliferation were very important event in wound healing process. The aims of this study were studying epithelization and fibroblas proliferation on incision wound healing process using pre treatment of  Tretinoin 0,1%  topically on mice. This research used 24 male mice, 3 month of age, with 150 -180 gram of body weight. The mice were divided into 2 groups, control group (K) and treatment group (P). The control group was a group of mice with an incision wound, while the treatment group was a group of mice with an incision wound that received a pretreatment of Tretionin of 0.1% topically. Each group was divided into 2 sub groups K4, K7, P4, P7 based on time of samples collecting. K4 and P4 were examined at  day 4, while K7 and P7 were examined at day 7. Epithelialization was observed by measuring the length of the epithelial incision area, whereas fibroblast proliferation was performed by counting the number of fibroblasts in five field of view on histopathologic skin preparations processed by HE staining.In this study,pretreatment of Tretinoin 0,1%  topically can increase epithelialization and number of fibroblast significantly (p<0,05).This increase may be due to 1% Tretinoin in the tissues to induce keratinocyte basal proliferation including its migration to the skin surface via retinoic acid receptors ( RARδ)Pretreatment of Tretinoin 0,1% topically can increased epithelialization and number of fibroblast on incision wound healing process of albino rats
Deteksi Johne’s Disease pada Sapi Potong di Kabupaten Kebumen Berdasarkan Uji Elisa Iip Zuliana Rachmawati; Widagdo Sri Nugroho; Rahmat Setya Adji
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6391.263 KB) | DOI: 10.22146/jsv.41154

Abstract

Kebumen Regency have been set as the source of the beef cattle breeds by the Ministry of Agriculture of the Republic of Indonesia. As the parent stock of beef cattle, these should be free from any infectious disease. Base on Balai Besar Veterinary Wates survey at 2015, some beef cattle in some districts at Kebumen indicated Johne's Disease. This research was carried out with the epidemiologic approach to detect Johne's Disease. About 173 serum specimens were taken from 173 heads of cows who have clinical symptoms of Johne's Disease. Sera were analyzed by ELISA. Base on ELISA result, 38 from 173 serum were seropositive. These cows sample come from local ancestor and there were not Johne’s disease vaccination program in Kebumen. This research proved that Johne's Disease have occurred in this populations which dedicated for parent stock of beef cattle in the District of Kebumen. Disease control program for Johne’s disease should be undertaken in this area to prevent the disease transmition.
Pengaruh fasciolosis pada sapi bali berdasarkan pemeriksaan darah, serum glutamic pyruvic transaminase (SGPT), dan alkaline phosphatase (ALP) Amirullah Amirullah; Dradjat AS; Sriasih M; Maskur Maskur; Depamede SN; Kisworo D; Kurniasih Kurniasih
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2010.199 KB) | DOI: 10.22146/jsv.42994

Abstract

Investation of Fasciola sp. On several species of cattle in Indonesia has occurred since a long time ago. Fasciolosis caused a high economical loss such as decreasing of carcass and organs, sometimes caused death of calves. The objectives of the research were to evaluate the effect of fasciolosis on Serum Glutamic Pyruvic Transaminase (SGPT), and  the obstruction of  bile duct by Alkaline Phosphatase (ALP) in Bali cattle suffered from fasciolosis. Faeces of Bali cattle raised in endemic areas of fasciolosis were examinated by sedimentation test to identify eggs per gram of faeces (EPG) of Fasciola sp. The blood samples of cattle which positive to fasciolosis were use to study the hematology by autoanalyser, SGPT  and ALP.  The results of research showed that from 89 cattle, 28 were infected by Fasciola sp. whithin of 7-115 EPG (min-max). From 20 fasciolosis cattle were indicated that MCHC of 90%, RBC of 30%, HB of 20%,  and  eosinophils of 2 % of cattle were lower than that of normal standard, while  lymphocytes of 60%, MCV of 40%, ALP of 10%  and SGPT of 5% of cattle were higher than that of normal standard. hematological appearances of cattle suffered from fasciolosis  had macrocytichypochromic anemia, eosinopenia, and lymphocytosis. The increase of SGPT may be caused byhepatic cells destruction by young liver flukes, and the increase of  ALP was caused by the obstruction of bile duct by mature liver flukes. 

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