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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

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Articles 16 Documents

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Search results for , issue "Vol 37, No 1 (2019): Juni" : 16 Documents clear

Peneguhan diagnosis Avian Influenza pada Ayam Petelur yang Mengalami Gejala Penurunan Produksi Rina Isnawati; Hastari Wuryastuti; R Wasito
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Decreasing egg production is an initial symptom of various diseases that infect commercial layer chickens. Diagnosis of diseases causing the decrease in egg production without high mortality rate is often thought to be caused by other poultry diseases because of the many similarities in manifestation between one disease and another. Avian influenza is a zoonotic infectious disease in poultry that can lead to high economic losses. This study aims to determine the contribution of avian influenza virus (AIV) infection to commercial laying hens showing symptoms of decreased production. The samples were oropharyngeal swabs of commercial laying hens which had symptoms of decreased production at productive age. Laboratory tests were carried out by histopathological examination, detection and identification of AIV with real time reverse transcriptase polymerase chain reaction (qRT-PCR) and isolation with the viral culture technique on fertile chicken eggs. The results showed that there was type A AIV infection based on the qRT-PCR (matrix) test, but the subtypes were not identified (H5, H7, H9). The results of the isolation of the virus in the fertile chicken eggs showed embryonic death in which all embryonic organs show hemorrhages. Allantoic fluid does not agglutinate chicken red blood cells. Histopathological features of the pathognomonic lesions in lungs infected with AIV were severe congestion and hemorrhages. Based on the results of the study, it was concluded that there was a contribution of type A AIV infection. Early diagnosis of AIV could determine more appropriate actions, including control, prevention and eradication.

PERBANDINGAN GAMBARAN DARAH ULAR SANCA BATIK (Malayophyton reticulatus) LOKAL JAWA DAN KALIMANTAN Slamet Raharjo; Sri Hartati; Sedarmanto Indarjulianto; Rini Widayanti
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Reticulated python (M. reticulatus) Java and Kalimantan locality highly demand as pet animal. Blood profile has an important role on animal health status. This research was aim to study the blood profile comparison of Java and Kalimantan M. reticulatus. Ten blood samples of Java M. reticulatus and 10 samples of Kalimantan M. reticulatus were used in this study. As much as 1 ml blood sample was collected from ventral coccygeal vein of adult healthy M. reticulatus. Blood sample put into tube with EDTA then analized at Internal Department Laboratory, Faculty of Veterinary Medicine UGM. Based on blood samples analize results, adult healthy Java M. reticulatus shows the higher value on parameters of erytrocyte, leucocyte, PCV, MCH, heterophyl, limphocyte and monocyte, while haemoglobin, TPP, MCV, MCHC, azurofil and monosit were lower than Kalimantan M. reticulatus. There were no significant differences (P>0,05) between blood profile of Java and Kalimantan M. reticulatus. It could be concluded that blood profile of Java and Kalimantan M. reticulatus is not different.

Distribusi neuron serotonergik pada hipokampus Lasiwen (Myotis sp) sebagai satwa liar yang berpotensi menjadi reservoir virus rabies Tri Wahyu Pangestiningsih; Ariana Ariana; Irma Padeta; Arvendi Rahma Jadi; Woro Danur Wendo
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Myotis sp is an Indonesian microchiropteran, insectivore bat that potential to be a reservoir for rabies virus. Rabies is fatal viral encephalitis and bat could act as wildlife potential reservoirs for the rabies virus and can transmit the disease to humans as the zoonotic disease. Hippocampus is one of diagnostic tissue for rabies disease and serotonergic neuron could involved in pathogenesis of rabies disease. The aim of the study is to investigate the distribution of serotonergic neurons in Myotis sp hippocampus. Five Myotis sp. were captured from wild population in Central Java, and were humanly anesthetized using ketamine and xylazin. The animals were perfused intracardially using NaCl 0.9% as the pre-rinse followed by 10% formaldehyde to fix it. The cerebrums were collected and processing the for paraffin embedding. Cerebrums were sectioning in saggital sections, 12 µm thickness serially with 120 µm intervals. The tissues were staining immunohistochemistry using antibody to serotonin (1/300; Bioss, Cat. No: bs-1126R) 2 night incubation in 4 oC temperature. The solution for blocking background, secondary antibody, avidin-biotin-peroxidae complex and chromogen using kit Starr Trek Universal HRP Detection System (Biocare Medical, Cat No: STUHRP700) and were analyzed descriptivelly. The results show that serotonergic neuros were distributed in the all area of the of Myotis sp hippocampus. In dentate gyrus neuron serotonergic (Sert-IR)s are round in shape and mostly distributed in the middle layer, few in the superficially also deeper layers. In the hippocampus , the Sert-IR neurons are pyramidal in shape and distributed in the CA1, Ca2 and CA3 areas. In subiculum, the Sert-IR neurons are pyramidal in shape, more wider distributed than in the CA1 with no differences between outer layer and deeper layer. The conclutions of this research are the serotonergic neurons are distributed in the all area of hippocampus

Evaluasi Pengobatan Trematodiasis Menggunakan Albendazol pada Sapi di Kecamatan Pakem, Sleman, Daerah Istimewa Yogyakarta Felisitas Kristiyani; Nurul Aini; Agustina Dwi Wijayanti
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The recent method of treatment for trematodiasis in cattle (age 1-10 years) using albendazol (10% of orally suspension) has deducted to evaluate the effectiveness of this anthelmintic to eradicate the trematoda infection especially Fasciola sp. and Paramphistomum sp. in Pakem subdistrict, Sleman district, Daerah Istimewa Yogyakarta Provence. These two species of trematodes were the mostly found in Yogyakarta area according to the annualy reports of Idul Qurban slaughter day. The cows used in this study were determined from three groups of farmers society at Pakem area. The physical examination and feces samplings for worm egg identification by Parfitt and Banks method were done as early examination to all populations. The positive results of Fasciola sp. and Paramphistomum sp. eggs of cows then grouped (Group A,B, and C) and treated with albendazole at dose of 10 mg/kg of body weight. All groups treated countinously for one year with albendazole; Group A repeated every two months, group B every four months, and group C every six months, respectively. Before administered the next dosing, the feces samplings and worm eggs identification were conducted to measure the effectiveness of albendazole that had applied. The results of treatments were the finding of trematoda eggs in feces then calculated descriptively. The effectiveness of albendazol of two months repetition was 31,25-100%, every four months was 38,47-46,15% and six months repeatly was 42,86%. It concluded that the highest effectiveness of albendazol to trematodiasis in cattle was two months repetition of treatment. The evaluation of albendazole treatments of cattle infected by Fasciola sp. and Paramphistomum sp. during 12 months resulted the low effectiveness because of only 30% of population that cured from infections. Keywords: cattle, albendazole, effectiveness, Fasciola sp., Paramphistomum sp. Abstrak Penelitian metode pengobatan trematodiasis pada sapi dewasa (umur 1-10 tahun) menggunakan albendazol (sediaan suspensi oral 10%) dilakukan untuk mengetahui metode terapi yang paling efektif terhadap infestasi cacing yang sering terjadi pada ternak sapi di Indonesia, khususnya di wilayah Sleman, Daerah Istimewa Yogyakarta. Infestasi cacing trematoda terutama Fasciola sp. dan Paramphistomum sp. cukup sering ditemukan di wilayah ini, dan sering ditemukan saat pemeriksaan tahunan penyembelihan hewan pada perayaan Idul Qurban. Sampel yang digunakan adalah sapi-sapi yang dipelihara di 3 kelompok ternak ( kelompok A, B, dan C) di wilayah Kecamatan Pakem, Kabupaten Sleman. Pemeriksaan fisik dan feses untuk identifikasi telur cacing dilakukan terhadap seluruh populasi di ketiga kelompok. Pengobatan yang dilakukan adalah dengan albendazol dosis 10 mg/kg berat badan, dengan metode ulangan untuk Kelompok A setiap 2 bulan sekali, Kelompok B tiap 4 bulan sekali dan kelompok C tiap 6 bulan sekali, selama satu tahun. Pemilihan sampel untuk pengamatan efektifitas obat cacing ditentukan pada sapi yang positif terinfeksi pada pemeriksaan pertama dengan ditemukan telur Fasciola sp. dan Paramphistomum sp. menggunakan metode Parfitt and Banks. Sebelum ulangan pengobatan, dilakukan pemeriksaan feses untuk mengukur efektivitas pengobatan sebelumnya. Berdasarkan data diketahui bahwa pengobatan albendazol dengan ulangan 2 bulan memberikan efektivitas berkisar 31,25-100%, ulangan 4 bulan 38,47-46,16% dan ulangan 6 bulan menghasilkan efektivitas sebesar 42,86%. Berdasarkan data tersebut disimpulkan bahwa efektivitas pengobatan menggunakan albendazol tertinggi diperoleh dengan cara mengulang pengobatan setiap 2 bulan sekali. Evaluasi terhadap seluruh populasi sapi yang terinfeksi Fasciola sp. dan Paramphistomum sp. selama 12 bulan menunjukkan rendahnya efektivitas albendazol karena hanya mampu menjaga ternak sapi dari infeksi kurang dari 30% populasi.Kata kunci : sapi, albendazol, efektivitas, Fasciola sp., Paramphistomum sp.

The Efficacy Study of Duramectin, Oxfendazole, Piperazine, and Pyrantel pamoate Against Gastrointestinal Worms In Horses In Yogyakarta Special Region*) Yuriadi Yuriadi; Ida Tjahajati; Soedarmanto Indarjulianto; Irkham Widiyono
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This research aimed at determining the efficacy of duramectin, oxfendazole, piperazine, and pyrantelpamoate against gastrointestinal worms in horses in Yogyakarta Special Region and Central Java. The object of research involved 40 horses diagnosed with gastrointestinal worm infection. Prior to the research, all of the horses were subjected to examination for clinical symptoms and parasitology laboratory checkup for signs of worm eggs in their feces, and they were pronounced positive for experiment animals with at least 150 eggs per gram of feces per horse. The research horses were weighed to determine the dose of worm medication to be used. The research horses are divided into four treatment groups, each group consists of ten horses. Group I was given duramectin with a dose of 0.2 mg/kg of body weight. Group II was given oxfendazole with a dose of 7.5 mg/kg of body weight, Group III was given 125 mg Piperazin treatment per kilogram of body weight and Group IV was given pyrantelpamoate with a dose of 20 mg/kg of body weight. After receiving themedication, the horses were observed to document the progress in terms of clinical symptoms and the amount and type of worm eggs in their feces every three days for four times in a row. The research data results were tabulated and were descriptive-comparatively analyzed.The research result showed varying efficacy level of worm medication for horses. Oxfendazole kills Strongylus and Parascaris worms. Duramectin kills Strongylus worms and reduce some of the Parascaris worms. Piperazineandpyrantelpamoate kills the Strongylus wormsand reduce some of the Parascaris.

Pelacakan eskpresi protein pada testis mencit (Mus musculus) setelah paparan esktrak etanol daun mimba (Azadirachta indica) Agung Janika Sitasiwi; Sri Isdadiyanto; Siti Muflichatun Mardiati
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Azadirachta indica (Neem) has been shown to affect the fertility of mice by interfering with the synthesis of testosterone in mice. The aim of this study was to detect the testes protein expression of mice after exposure to the ethanolic Neem leaf extract. The laboratory animals of this study were 20 male Swiss Webster mice with three months in age and body weight ranging from 27.5 grams. The mice were divided into two treatment groups, namely K (control group, exposed with distilled water) and P (treatment group, exposed to etahnolic Neem leaf extract with 14 mg/animal /day). The treated were given for 21 days and the testicular protein was carried out on the 22nd day. The variables observed were testes weight, concentration and expression of proteins isolated from the testes. The protein concentration is determined by a spectrophotometer at a wavelength of 450nm. The protein expression was observed and determined based on the results of protein electrophoresis (SDS-PAGE). The results showed that protein expression in the treatment group has a lower concentration compared to the control group. Those results was confirmed by thinner bands in SDS-PAGE result. Those proteins thought to be a fertility determinant in mammals. Keywords : anti-fertility; Neem; protein expression Abstrak Azadirachta indica (Mimba) telah terbukti mempengaruhi fertilitas mencit dengan cara mengganggu sintesis hormon testoteron pada mencit. Penelitian ini bertujuan untuk melacak ekspresi protein pada testis mencit setelah paparan ekstrak etanol daun Mimba. Hewan uji penelitian ini adalah 20 ekor mencit Swiss Webster jantan dengan umur tiga bulan dan bobot badan berkisar 27.5 gram. Hewan uji dibagi menjadi 2 kelompok perlakuan, yaitu K (kelompok kontrol, dipapar akuades) dan P (kelompok perlakuan, dipapar dengan ekstrak etanol daun Mimba dengan dosis 14 mg/ekor/hari). Pemberian bahan uji dilakukan secara oral selama 21 hari. Variabel yang diamati adalah bobot testes, konsentrasi serta eskpresi protein yang diisolasi dari testis. Isolasi protein testis dilakukan pada hari ke-22. Konsentrasi protein ditentukan dengan spectrofotometer pada panjang gelombang 450nm. Ekspresi protein diamati dan ditentukan berdasar hasil elektroforesa protein. Hasil penelitian menunjukkan bahwa ekspresi protein pada kelompok perlakuan menunjukkan konsentrasi yang lebih rendah dengan pita yang lebih tipis jika dibandingkan dengan kelompok kontrol. Kesimpulan penelitian ini adalah paparan ekstrak etanol daun Nimba menyebabkan gangguan ekspresi protein yang diduga berperan dalam menentukan fertilitas mamalia. Kata kunci : Mimba; anti-fertilitas; eskpresi protein;

Uji Ekstrak n-Hexana Rumput Kebar (Biophytum petersianum Klotzsch) pada Tikus Wistar Hiperkolesterolemia Priyo Sambodo; Purwaningsih Purwaningsih; Alnita Baaka; Trini Susmiati; Claude Mona Airin
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was aimed to determine the secure level of the n-hexane extract of kebar grass on hypercholesterolemia rats based the acute oral toxicity test. Based the literature, no report on the toxic dosage before, initial dose was started at 300 mg/kg body weight (BW) and to be increased 2000 to 5000 mg/kg BW. Toxicity data were set for 24 hours and continued until 14 days after treatment for several parameters, i.e : toxicity symptoms, death of animals, changes in body weight and the manifestation of toxicity effects. At the end of the test, the rats were sacrificed and organs were taken for abnormalities (macroscopy). The results of this study showed that the administration of the n-hexane extract of grass kebar at all doses did not show the toxicity symptoms, mortality and body weight change. The absolute and relative weights and gross pathology observation of the internal organ were normal. Conclusions: the n-hexane extract of grass kebar is safe and the LD was determined on category 5, 50 unclassified or minimum practically non toxic

Studi Reseptor Virus Infectious Bursal Disease (IBD) pada Organ Limfoid Ayam Pasca Vaksinasi dengan Metode Imunohistokimia Restu Librani; Agus Setiyono; Wiwin Winarsih
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Infectious bursal disease (IBD) affects economical impact for breeders due to it can cause damage lymphoid organ, especially bursa of Fabricius and causing failure the vaccination program. Infectious bursal disease virus receptors on lymphoid organs suspected contribute to subclinical and clinical IBD incidence in the chickens. The aim of this study was to determine and compare the distribution of IBDvirus receptors on lymphoid organ of the chicken which obtained different IBD vaccination program. The presence of virus receptors in bursa of Fabricius, spleen, and thymus were observed microscopically using immunohistochemical method and evaluated with Image J® software. Monoclonal anti LSCC-BK3 (Gifu University, Japan) antibodies as primary antibody was used in this study. The result showed that IBDvirus receptors found abudantly in the bursa of Fabricius, afterwards in the spleen and thymus. No significant differences of IBD virus receptor distribution within lymphoid organs between chicken which obtained once and twice IBD vaccination. Infectious bursal disease virus receptor distribution in bursa Fabricius chickens aged 23 days received twice IBD vaccination more than once IBD vaccination program.

Isolasi, Identifikasi, dan Serotyping Avibacterium paragallinarum dari Ayam Petelur Komersial yang Menunjukkan Gejala Snot Elisabet Tangkonda; Charles Rangga Tabbu; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Infectious coryza (snot) is one of acute respiratory disease in breeders, layers, and broilers caused by Avibacterium paragallinarum (Av. paragallinarum). This disease is very harmful because of its cause decrease in egg production and high morbidity. Snot incident in Indonesia still is reported. Vaccination is one of the best preventive measures, but reports about Av. paragallinarum serotype at the field so lack so that the correspondence between serotype Av. paragallinarum in the field to those used for vaccination is unknown. Av. paragallinarum has strains with different antigenicity and until now known three serotypes there are serotypes A, B and C. Serotypes A and C are pathogenic then serotypes B. The purpose of this study was to isolate, identify and to determine the serotype of Av. paragallinarum from the commercial layer that showing symptoms of snot. Samples were taken from layer which showed symptoms of snot (nasal foul smelling exudate, infra-orbital and wattle swelling, conjunctivitis, and snoring) from some layer farms. Samples were cultured on chocolate agar and then incubated in a candle jar at 37 ᴼC for 18-24 hours. Bacteria colony and cell morphology were observed and performed biochemical tests (catalase, oxidase, urease, indole, and fermentation of carbohydrates) in suspected colonies. Serotypes test was conducted using plate agglutination test (PAT). This study revealed 4 isolates Av. paragallinarum with 2 isolates are serotype B and 2 other are serotype C.

Pengaruh Pemberian Serum Albumin Manusia terhadap Kadar Albumin dalam Darah pada Anjing dengan status Hipoalbuminemia Julita Dewitri Merthayasa; Putu Devi Devi Jayanti; Soedarmanto Indarjulianto; Ryan Hadi Permana; Nadia Liswardani Destinanda; Agustina Dwi Wijayanti
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Albumin consists of 50% of the total plasma protein and it is very important protein in the animal's body. Hypoalbuminemia can occurs due to various conditions, such as loss of protein nephropathy, loss of enteropathic proteins, chronic and acute diarrhea or heavy bleeding as well as malnutrition and malabsorption conditions. This study aims to determine the effect of human serum albumin 20% infusion to increase albumin blood level in hypoalbuminemia dogs. Animals used in this study were 5 dogs whose results showed the levels of albumin below normal limits or hypoalbuminemia. Dogs with hypoalbuminemia will be infused with human serum albumin (HSA) 20% through intravenous for ± 4 hours. Then, these dogs blood will be drawn days after infusion to screen for albumin levels. The results obtained in the form of examination data of albumin blood levels in the blood before and after infusion will be analyzed with paired T-test. The results showed an increase of albumin level significantly from an average of 1.98 ± 0.356 to 2.28 ± 0.257(P <0.05). This study shows the presence of human albumin serum through intravenous infusion was able to increase albumin levels in dogs with hypoalbuminemia.

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