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Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 24 Documents
 1   2   3 
Search results for , issue "Vol 38, No 1 (2020): April" : 24 Documents clear
The Conjecture of Causa Mortis In Jenkins’ Whipray Pateobatis jenkinsii (Annandale, 1909): A Case Report Rizkiantino, Rifky; Binol, Ridzki M. F.
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1775.57 KB) | DOI: 10.22146/jsv.57370

Abstract

Seekor ikan pari cambuk Jenkins jantan tangkapan liar ditemukan mati dalam tangki karantina dengan gejala klinis sebelum kematian adalah nafsu makan berkurang selama seminggu. Riwayat pengobatan yang diberikan adalah pemberian antibiotika enrofloxacin secara peroral. Periode terapi berlangsung selama sepuluh hari. Pengobatan terakhir adalah pemberian Hepavit® (ekstrak hati) dan injeksi intramuskular (IM) antibiotika enrofloxacin. Satu hari sebelum kematian, sampel darah dikoleksi dan kemudian diperiksa untuk mengetahui gambaran hematokrit dan beberapa parameter kimia darah. Hasil pemeriksaan darah ditemukan penurunan kadar blood urea nitrogen (BUN), alkaline phosphatase (ALP), dan alanine aminotransferase (ALT), peningkatan kadar glukosa, penurunan total protein dan kadar albumin, serta peningkatan kadar globulin. Pemeriksaan patologi anatomi ditemukan lesi pada ekor, di sekitar mata, dan clasper. Lesi hemoragik ditemukan di lapisan mukosa esofagus, lambung, dan kolon spiral. Gumpalan darah ditemukan di bawah lapisan tunika organ testis. Kerusakan organ hati secara makroskopis ditunjukkan dengan perubahan warna yang tidak homogen, pembengkakan organ, kongesti hati, dan konsistensi yang rapuh. Berdasarkan diagnosis morfologik, kausa kematian ikan diduga karena mengalami kondisi infeksi septikemia selama beberapa minggu sebelumnya.
Bovine Ephemeral Fever (BEF) : Penyebab, Epidemiologi, Diagnosa, dan Terapi Nururrozi, Alfarisa; Indarjulianto, Soedarmanto; Yanuartono, Yanuartono; Hary Purnamaningsih, Hary Purnamaningsih; Rahardjo, Slamet; Rusmihayati, Rusmihayati
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1226.791 KB) | DOI: 10.22146/jsv.57372

Abstract

Bovine ephemeral fever (or 3-day sickness) is an acute febrile illness of cattle and water buffaloes caused by an Ephemerovirus of the family rhabdoviridae. The disease transmitted by arthropod vectors. It is common in tropical and subtropical regions. The main impact of BEF infection cause decreased productivity, decreased milk yield, body score and reproductive losses, and recovery can be prolonged in some animals. Clinical signs vary in individual animals, but the classic course begins with a fever, which is often biphasic to polyphasic. Mortality is typically low, however, significantly higher case fatality rates were reported in some recent outbreaks. This disease is widespread in various regions in Indonesia. In general, BEF does not cause large economic losses if medical assistance is provided sufficiently so that complications do not occur with other diseases. This paper aims to summarize the causes, epidemiology, diagnosis methods, and treatments that can be done to overcome BEF disease.
Profil Darah Rusa Totol (Axis axis) Betina Sehat di Pusat Inovasi Agroteknologi (PIAT), Universitas Gadjah Mada Dhirgo Adji; Dian Astuti
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.28204

Abstract

Center for Agrotechnology Innovation, Gadjah Mada University (PIAT), one of the place for development and breeding ground of spotted deer in Yogyakarta, Indonesia. The study of blood profiles was intended to allow veterinarian to understand the normal profile of the spotted deer. Fifteen healthy spotted deer belonging to PIAT were used as experimental animals. The deer were drawn its blood through the jugular vein without the use of anaesthesia. Blood was then accommodated in an EDTA tube, centrifuged at 2500 RPM and analyzed using a Mindray BC-2800 haematology analyzer machine. Based on the results of the study of the blood it were known that  Haemoglobine (Hb) was: 11.5 ± 1.703 g/dl, Red blood cells (RBC) was: 9.3 ± 3.580 106/ml, Packed cell volume (PCV) was: 30.8 ± 6.035 %, Mean corpuscular Volume (MCV) was: 36.8 ± 11.102 fl, Mean corpuscular haemoglobine (MCH) was: 15.0 ± 7.313 pg, Mean corpuscular haemoglobine concentration (MCHC) was: 40.0 ± 14.657 g/dl, White blood cells (WBC) was: 6.4 ± 3.096 103/ml , Neutrophils was: 43.4 ± 21.646, % Basophils was: 0.2 ± 0.168 %, eosinophils was: 0.4 ± 0.447 %, lymphocytes was: 53.4 ± 21.546 % and monocytes was: 2.6 ± 2.394 %. From all of the datas it indicated that blood profiles of PIAT’s spotted deers are different from similar spotted deer blood profiles in India.
KADAR SENYAWA TOKSIK DALAM DARAH IKAN NILA (Oreochromis niloticus) SAAT MUSIM UP-WELLING DI DANAU MANINJAU, SUMATRA BARAT Ade Dewi Maharani; Silvi Susanti; Fachrul Reza
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.29322

Abstract

Maninjau lake is the largest center for the freshwater floating-net-cage fisheries system in West Sumatra. Every year, during the up-welling season, a huge number of fish suffered to death in the lake including nile tilapia (Oreochromis niloticus), a common species cultivated in the floating-net cages. This current study aimed to determine the factors contributing to the death of fish by measuring the concentration of toxic chemical substances in the blood plasm of nile tilapia as well as in the subtrate collected from the bottom of the lake. The samples were collected in the sites with higher number of floating-net cages as well as higher number of mass death of fish (Tanjung Sani, Pakan Raba’a dan Maninjau) during the up-welling season. The result revealed that the levels of phosphate, nitrate, nitrit, and amonium were higher in the blood plasm of the fish. Moreover, the levels of those chemical substances were also higher in the substrate of the lake. The higher concentration of the toxic chemical substances might contribute to the mass death of fish living in the floating-net cages during the up-welling season.
Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh (Antibiotic Activities To Staphylococcus aureus Biofilms Of Aceh Cattle Preputium Isolate) Maryulia Dewi; Darmawi Darmawi; Nurliana Nurliana; T. Fadrial Karmil; T Zahrial Helmi; Fakhrurrazi Fakhrurrazi; Erina Erina; Mahdi Abrar; M. Daud AK; Masda Admi
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.35219

Abstract

AbstractBiofilm  is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB)  medium were used to biofilm. Bacterial  while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were  λ 595 nm. Data were analyzed by using Analysis Of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/μL. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/μL. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive. Keywords: biofilm, antibiotics, Staphylococcus aureus, cows aceh.                Abstrak Biofilm merupakan salah satu parameter untuk menentukan jalur patogenisitas suatu bakteri. Antibiotik dapat melemahkan aktivitas virulensi bakteri dengan menghambat pembentukan biofilm. Penelitian ini bertujuan untuk melihat karaksteristik dan aktivitas antibiotik terhadap biofilm Stapylococcus aureus isolat preputium sapi Aceh. Penelitian bersifat eksplorasi dan eksperimental, menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan menggunakan tiga jenis antibiotik, tiga konsentrasi dan tiga kali ulangan. Antibiotik yang digunakan adalah tetrasiklin, oksitetrasiklin dan fosfomisin dengan konsentrasi masing-masing 10%, 20% dan 30% (mg/mL). Pengujian biofilm dilakukan pada media Nutrient Broth (NB), menggunakan mikroplat U-bottom polystyrene 96 wells. Pewarnaan biofilm menggunakan kristal violet 1%. Optical Density (OD) biofilm dibaca pada    λ 595 nm. Data hasil penelitian dianalisis dengan menggunakan Analysis Of Variance (ANOVA). Hasil penelitian menunjukkan aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm tergolong positif moderat, yaitu 2,667cell/µL. Uji Biofilm menunjukkan jenis dan konsentrasi antibiotik berpengaruh secara nyata (P<0,05) terhadap daya hambat biofilm Staphylococcus aureus isolat preputium sapi Aceh. Tetrasiklin 30% (mg/mL) mempunyai kemampuan paling baik menghambat pembentukan biofilm Staphylococcus aureus isolat preputium sapi Aceh sebesar 52,18% menjadi 1,262 cell/µL, sehingga aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm dari positif moderat menjadi positif palsu. Kata Kunci: biofilm, antibiotik, Staphylococcus aureus, sapi aceh.
Bacterial Detection Causing Subclinical Mastitis on Etawah Crossbreed Goat in Kokap, Kulon Progo, Yogyakarta Province Clara Ajeng Artdita; Morsid Andityas; Nur Ika Prihanani; Yohanes Wawan Budiyanto
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.39818

Abstract

Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria,  manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%). 
Bovine Ephemeral Fever (BEF) : Penyebab, Epidemiologi, Diagnosa, dan Terapi Alfarisa Nururrozi; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Hary Purnamaningsih; Slamet Raharjo; Rusmihayati Rusmihayati
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.41863

Abstract

Bovine ephemeral fever (atau 3-day sickness) adalah penyakit demam akut pada sapi dan kerbau yang disebabkan oleh Ephemerovirus dari keluarga rhabdoviridae dan ditularkan oleh vektor arthropoda. Penyakit tersebut biasa terjadi di daerah tropis dan subtropis. Dalam banyak kasus, dampak utamanya adalah pada penurunan produktivitas, produksi susu, kondisi tubuh, gangguan reproduksi, dan periode pemulihan yang lama pada beberapa hewan. Gejala klinis bervariasi pada setiap individu hewan, tetapi pada umumnya diawali dengan demam yang bersifat bifasik melanjut menjadi polifasik. Mortalitas biasanya rendah, namun, peningkatan kasus berakibat fatal telah dilaporkan dalam beberapa wabah akhir-akhir ini. Penyakit ini tersebar luas diberbagai daerah di Indonesia. Secara umum tidak menimbulkan kerugian ekonomi yang besar, asalkan segera mendapatkan pertolongan medis yang memadai sehingga tidak terjadi komplikasi dengan penyakit lain. Tulisan ini bertujuan untuk mengulas penyebab, metode diagnosa, dan terapi yang dapat dilakukan untuk menanggulangi kejadian BEF.
PENILAIAN ORGANISASI PERANGKAT DAERAH (OPD) TENTANG OTORITAS VETERINER DALAM KEBIJAKAN PENANGGULANGAN RABIES DI PROVINSI BANTEN Joko Ismadi; Bambang Sumiarto; Widagdo Sri Nugroho; Erwan Agus Purwanto
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.42644

Abstract

AbstractLegislation No 41 Year 2014 about Amendment of Legislation No 18 Year 2009 about Livestock and Animal Health explains that Veterinary Authority is a government institution, formed in order to deal with decision making of anything related to animal health, by involving veterinarians and mobilizing all related professional lines, to identify problems, deciding policies, coordinating policy implementation, and handling the field operations.This research material uses the result from a group of respondents, which represents 36 Regional organizations (OPD), they are: 9 OPD Technical services, supervising animal health (keswan) and veterinary public health (kesmavet), 9 OPD Health services, 9 OPD Regional development planning services, and 9 OPD Regional staffing services (BKD), for regencies, and cities in Banten Province. Data collection is done using two methods, quantitatively by using data from all 36 OPD, and qualitatively by using observation, interviews, dan questionnaires.First 5 questions: 1). Are the organization bureaucratic? 2). Positions, roles, and authority of veterinarians? 3). Is the veterinary authority regarding rabies control at agencies, clinics, and animal health center already in line with the implementation? 4). Strategic policy and 5). Integrated, directed, and continuous program regarding public awareness of rabies, for rabies control in provincial or regency government. The answers are as follows, Technical services of animal health (keswan) and veterinary public health (kesmavet) : 0%, 33,33%, 77,78%, 100% and 77,78% answered yes; 100%, 66,67%, 22,22%, 0% and 22,22% answered no. Health services: 22,22%, 66,67%, 88,89%, 44,44% and 44,44% answered yes; 77,78%, 33,33%, 11,11%, 55,56% and 55,56% answered no. Regional development planning services (Bappeda), 33,33%, 88,89%, 66,67%, 11,11% and 11,11% answered yes; 66,67%, 11,11%, 33,33%, 88,89% and 88,89% answered no; Regional staffing services (BKD) 88,89%, 77,78%, 55,56%, 0% and 0% answered yes; 11,11%, 22,22%, 44,44%, 100% and 100% answered no. Next questions: 6). Cooperations between OPD, 7). Regional Policy 8). Veterinarian openings and 9). Enforcement and improvement of otovet institutions. The answers are as follows: Technical services of keswan kesmavet: 33,33%, 44,44%, 77,78% and 100% answers yes; 66,67%, 55,56%, 22,22% and 0% answers no. Health services: 55,56%, 22,22%, 0% and 77,78% answers yes; 44,44%, 77,78%, 100% and 22,22% answers no. Regional development planning services (Bappeda), 33,33%, 33,33%, 33,33 and 100% answers yes; 66,67%, 66,67%, 66,67% and 0% answers no; while Regional staffing services (BKD) 11,11%, 11,11%, 100% and 66,67% answers yes, 88,89%, 88,89%, 0%, !00% and 33,33% answers no.In conclusion, after scoring the questionnaire, the results for OPDs supervising animal health (keswan) and veterinary public health (kesmavet) otovet are as follows:  Technical services with 9 OPDs, 4 OPDs scores well and 5 OPDs scores poor. Health services with 9 OPDs, 4 OPDs scores well and 5 OPDs scores poor. Regional development planning services (Bappeda) with 9 OPDs, 4 OPDs scores well and 5 OPDs scores poor. Regional staffing services (BKD) with 9 OPDs, 1 OPD scores well and 8 OPDs scores poor.Keywords: Veterinary authority; Regional organizations (OPD), control rabies, rabies
Pengaruh Ukuran Ovarium dan Folikel Terhadap Penampilan Reproduksi pada Sapi Po dan Simpo di Kecamatan Jatinom, Kabupaten Klaten Topas Wicaksono Priyo Jr; Agung Budiyanto; Asmarani Kusumawati
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.43960

Abstract

The increasing beef cattle population in Indonesia is not significantly with high demand of meat every year. The insignificant increasing in population is caused by fertility decreasing, infectious disease and the reduction amount of forage land. The factor which causes cattle fertility decreasing can’t be separated from the ovarian and follicular diameter which have an effect on high various reproductive appearance. Parameters that use for knowing reproductive appearance of cattle are Service per Conception (S/C) and Calving Interval (CI). The aims of this study is to describe ovary sizes and follicular diameter in cattle, to describe ovary and follicular diameter toward S/C and CI variation. This research uses female cattle, 8 SimmentalPO (SimPO) and 9 Ongole breeds (PO), multiparous, are not pregnant, 4-8 years old, Body Condition Score (BCS) 2.5-3.5, peak phase estrus, has no reproductive problems, clear recordings and live in the area of farmer in Jatinom District, Klaten Regency, Central Java Province. Cattle are examined for ovarian size and follicle size using ultrasonography. S/C and CI data were obtained from records of artificial insemination cards (AI). The data obtained were analyzed by t test. The results of this study showed there were no differences in the diameter of ovaries in the size of SimPO and PO cattle (P> 0.05), there were no differences in the diameter of SimPO and PO cattle follicles (> 0.05), there were differences in ovarian and follicular diameter sizes with respect to S/C and CI in cattle with good and bad reproductive performance (P <0.05). The conclusion of the study showed that there was no difference in ovarian and follicular diameter size in SimPO and PO cattle (P> 0.05) but there was an influence on S/C and CI (P <0.05).
Virulence Characteristic of Avian Pathogenic Escherichia coli (APEC) Isolates Sruti Listra Adrenalin; Lynda Nugrahaning Imanjati; Ima Fauziah; Vinsa Cantya Prakasita; Sitarina Widyarini; Agnesia Endang Trihastuti Wahyuni
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.46494

Abstract

Avian pathogenic Escherichia coli (APEC) is a cause of colibacillosis in poultry, one of the respiratory disease that causes serious problems in the poultry industry. The APEC can cause high mortality and culling, decreased production, and high costs of treatment. Manifestations of colibacillosis are airsacculitis, perihepatitis, and pericarditis. The APEC serotypes that are widely identified in the field are O1K1, O2K1, and O78K80. Embryo lethality assay (ELA) is a method for determine the virulence of APEC serotypes. The aim of this study was to determine the virulence characteristic of APEC isolates. Five APEC serotypes O1K1, O2K1, O78K80, O157H7, and unknown serotype were used for ELA method by inoculated E. coli into chorioallantoic of specific pathogen free 12-days old embryos. Each group of 10 embryos, inoculated E. coli dose of 100-500 CFU/ 0,1 ml. Candling was carried out for 6 days (18-days old embryo) to determined the mortality and pathological lesions. The percentage of embryo mortality post-inoculated with APEC O1K1, O2K1, unknown serotypes were 100% (10/10), O78K80 serotype was 90% (9/10), and O157H7 serotype was 70% (70%). Lesions of all embryos were cranial and extremity hemorrhage. In this study, E. coli isolates had high virulence. 

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