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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 12 Documents
 1   2 
Search results for , issue "Vol 38, No 2 (2020): Agustus" : 12 Documents clear
Pengembangan Media Transpor untuk Koleksi Sampel Preputium, untuk deteksi Bovine Genital Campylobacteriosis Apris Beniawan; Agustin Indrawati; Fachriyan Hasmi Pasaribu
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.25912

Abstract

Campylobacter fetus subsp. Venerealis (Cfv) is bacteria causing contagious genital diseases in cows called Bovine Genital Campylobacteriosis (BGC) or vibriosis. Isolation of Cfv is difficult, because the bacteria are fragile and need specific nutrients and oxygen (5-10%). The transport media is very important to maintain Cfv survival before culturing in laboratory. The aim of this study was to modify a new transport media as an alternative media for Cfv. Developed media capability was compared to Weybridge media, and Phosphate Buffered Saline (PBS). All transport media was contaminated by Cfv with concentrations of 105,104,103,102,101 (CFU/ml), and was stored for <6, 24, 48, 72, and 96 hours in each transport medium before culturing on blood agar, all in triplicate. The quality of transport media was analyzed based on bacterial growth on blood agar. PCR test was used as a confirmatory test of growing bacteria cultured on blood agar. Based on culture results, Cfv stored in three transport mediums for <6 hours, Cfv grew on blood agar from all concentration levels provided. Cfv stored for 24, 48, 72 and 96 hours on PBS did not grow, whereas on developed media and Weybridge media, the bacteria could grow and enrichment occurs at all concentration levels given. This study indicated the developed media can be used as an alternative transport medium for Cfv bacteria.
PLATELET RICH PLASMA (PRP) DARI LIMBAH DARAH SAPI SEBAGAI OBAT LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus) Rahmad Dwi Ardhiansyah; Riefky Pradipta Baihaqie; Muhammad Nuriy Nuha Naufal; Muhamad Atabika Farma Nanda; Aprilia Maharani; Yuda Heru Fibrianto
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.32631

Abstract

Luka bakar adalah kerusakan jaringan pada kulit akibat terpajan panas tinggi, bahan kimiawi maupun arus listrik. Salah satu hewan yang disembelih di Rumah Potong Hewan (RPH)  adalah sapi. Seekor sapi dapat menghasilkan  limbah  darah kurang lebih sebanyak 28 liter. Komponen darah terdiri dari plasma, sel darah merah, sel darah putih dan platelet. Platelet mengandung growth factor. Platelet Rich Plasma (PRP) atau plasma yang kaya akan platelet terbukti dapat mempercepat penyembuhan tulang dan jaringan lunak. Pembuatan PRP dari darah sapi dilakukan dengan metode sentrifuge. Hewan coba  yang dipakai pada penelitian ini adalah tikus putih sebanyak 10 ekor. Besi panas berbentuk bulat dipanaskan dengan api, lalu ditempelkan pada kulit. Kelompok perlakuan diberi PRP dengan konsentrasi 10%, 20% dan 30% dengan salep vaselin album, kelompok kontrol negatif yang tidak diberi perlakuan dan kelompok kontrol positif yang diberi obat yang terbuat dari ekstrak plasenta sapi 10%+neomycin sulfate 0,5%. Parameter yang dipakai untuk mengukur tingkat kesembuhan luka adalah waktu kesembuhan dan pemeriksaan histopatologi. Pada hasil pengukuran waktu kesembuhan dianalisis dengan metode statistik One-way Analsisis of variance (Anova) tingkat signifikansi 95% dengan Kruskal-wallis, kesembuhan luka terdapat perbedaan signifikan antara PRP konsentrasi 10%, 20%, 30%, dan kontrol positif dengan kontrol negatif. Konsentrasi PRP yang optimal adalah 20%. Analisa tipe jaringan yang terbentuk dilakukan dengan pembuatan preparat histopatologi, hasil menunjukkan bahwa luka pada kelompok perlakuan dan kontrol positif  tampak sembuh, sedangkan kelompok kontrol negatif epitelisasi belum tertutup jelas. PRP merupakan obat penyembuh luka bakar yang efektif sebagai pengganti obat komersil yang sudah tersedia.
Karakter Morfologi Rambut Kelompok Cervidae Indonesia Ni Luh Putu Rischa Phadmacanty; Zulkurnia Irsaf; Gono Semiadi
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.47456

Abstract

Identification through animal hair character is one of a very important forensic tool given the high level of animal trade in Indonesia, one of which is the deer family (Cervidae). Indonesia has four species of Cervidae, there are Rusa timorensis (Javan deer), Rusa unicolor (Sambar deer), Muntiacus muntjak (Barking deer) and Axis kuhlii (Rusa Bawean). Until now, no information about the morphological character of Indonesian cervidae’s hair. In this study, we used 30 shaft/individual/species from Javan deer (8 individuals), Sambar deer (5 individuals), Barking deer (5 individuals) and Bawean deer (5 individual) from specimens collection of Museum Zoologicum Bogoriense (MZB) and field collections. Hairs were analyzed for macroscopic and microscopic, with several parameters of morphology, cuticular structure, medula, cross-section, and medullary index. The result showed that the special character of this family was filled lattice medulla structure and can be used for species  identification.
HUBUNGAN KEPADATAN PERMUKIMAN DENGAN LUAS PERMUKIMAN TERHADAP SEBARAN DEMAM BERDARAH DENGUE Marlena Marlena; Rinidar Rinidar; Muhammad Rusdi; Farida Farida; Teuku Reza Ferasyi; Nurliana Nurliana
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.47774

Abstract

Kota Banda Aceh merupakan daerah endemis penyakit Demam Berdarah Dengue (DBD). Kasus DBD di Banda Aceh selalu fluktuatif dari tahun ke tahun sehingga perlu dianalisis hubungan lingkungan terhadap sebaran DBD. Tujuan dari penelitian ini adalah untuk menganalisis hubungan kepadatan permukiman dengan luas permukiman terhadap sebaran demam berdarah dengue (DBD) di Kota Banda Aceh. Penelitian ini menggunakan metode interpretasi visual dan overlay dengan menggunakan rancangan penelitian deskriptif kuantitatif. Penelitian deskriptif dilakukan dengan metode survei berdasarkan studi cross-sectional. Survei dilakukan terhadap kepadatan permukinan dan dikaitkan dengan kejadian demam berdarah dengue (DBD) secara spasial. Jenis data yang digunakan adalah data primer diperoleh melalui observasi langsung menggunakan alat Global Positioning System (GPS) untuk melihat sebaran DBD dan data interpretasi citra penginderaan jauh untuk melihat pola kepadatan permukiman. Sedangkan data skunder dari Dinas Pekerjaan Umum dan Penataan Ruang Kota Banda Aceh dan Dinas Kesehatan Kota Banda Aceh. Jumlah sampel adalah seluruh penderita DBD tahun 2017 berjumlah 236 orang yang tersebar di Kota Banda Aceh. Data di analisis menggunakan ArcGIS dan diolah secara statistik menggunakan Chi-Square. Daerah kepadatan permukiman jarang sebesar 46,7% memiliki 3 titik kasus DBD katagori tinggi, daerah kepadatan permukiman sedang sebesar 34,4% memiliki 7 titik kasus DBD katagori tinggi dan daerah kepadatan permukiman padat sebesar 18.9% memiliki 5 titik kasus DBD katagori tinggi. Hasil uji statistik memperlihatkan nilai P>0,05, artinya tidak ada hubungan kepadatan permukiman dengan kejadian kasus DBD di Kota Banda Aceh.Tidak terdapat hubungan kepadatan permukiman dengan luas permukiman terhadap sebaran demam berdarah dengue (DBD) di Kota Banda Aceh.
GAMBARAN LEUKOSIT KUCING PENDERITA FELINE PANLEUKOPENIA Hary Purnamaningsih; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Alfarisa Nururrozi; Irkham Widiyono; Rusmi Hayati
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.50202

Abstract

Salah satu penyakit pada kucing dengan morbiditas dan mortalitas tinggi adalah Feline Panleukopenia (FPL). Tujuan dari penelitian ini adalah mengetahui  gambaran leukosit  kucing penderita Feline Panleukopenia. Penelitian ini menggunakan 27 ekor kucing jantan dan betina berbagai umur yang didiagnosa FPL berdasar Feline Parvo Virus Ag test. Semua kucing diambil darah secara lege artis sebanyak 1 ml, diperiksa jumlah leukositnya, kemudian dianalisis secara diskriptif. Hasil penelitian menunjukkan bahwa FPL lebih banyak diderita kucing jantan (59,3 % ) dari pada betina (40,7%). Kejadian FPL lebih banyak diderita kucing umur ≤ 6 bulan, yaitu 21 ekor (77,8 %) dibanding umur > 6 bulan, yaitu 6 ekor (22,2 %). Sebanyak 19 ekor (70,4 %) FPL mempunyai jumlah total leukosit < 1.000 sel/mm3, 4 ekor (14,8 %) 1.000 – 2.500 sel/mm3 dan 4 ekor yang lain (14,8 %) > 2.500 sel/mm3. Berdasarkan hasil penelitian ini disimpulkan bahwa sebagian besar penderita Feline Panleukopenia mengalami penurunan leukosit berat dengan prognosis infausta, terutama pada kucing jantan dan umur muda.
Klasterisasi Staphylococcus aureus Resisten Neutrofil Berdasar Assesory Gene Regulator Santosa, Christin Marganingsih; Lestari, Fajar Budi; Widayanti, Rini; Salasia, Siti Isrina Oktavia
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.50653

Abstract

Staphylococcus aureus is recognized worldwide as a major pathogen causing subclinical intramammary infections in dairy cows and food poisoning due to its ability to produce enterotoxin. The study aimed to identify enterotoxins of S. aureus and clustering the enterotoxins based on assessory gene regulator (agr). Virulence of S. aureus to the host was characterized based on the response of polymorphonuclear cells to the infection. Twelve S. aureus could be isolated from milk cows in central of dairy farming in Sumedang West Java. The identification of S. aureus was based on cultural and biochemical tests and an amplification of a specific section of the 23S rRNA gene. The sensitivity test against antibiotics revealed that some isolates of S. aureus were resistant to penicillin and methycillin. By PCR amplification one or more staphylococcal enterotoxin genes could be observed five genes in combinations of sea (216 bp), seb (478 bp), seh (375 bp), sei (576 bp), and sej (142 bp). Clustering of S. aureus based on the assesory gene regulator could be grouped into 4 clusters for agr1 (1 isolat), agr2 (2 isolates), in combination for agr1 and agr2 (1 isolate), and for non agr (2 isolates). Based on the response of polymorphonuclear cell in vitro and in vivo assays, revealed that S. aureus strain I-2 (agr1 cluster) and P1 (agr1+agr2 cluster) were more resistant to polymorphonuclear cells and could survive intracellularly, indicated that these strains could be used as proper candidates to develop dignostic tool based on agr against staphylococcal mastitis.  
Effectiveness of Gout Therapy with Polyherbal Extract of Shallot (Allium ascalonicum L.) and Red Ginger (Zingiber officinale var rubrum) in Hyperuricemia Mice Fathur Rohman Haryadi; Dela Ria Nesti; Ida Tjahajati; Okti Herawati
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.51360

Abstract

Gout can cause inflammation of acute gout arthtritis, as well as other complications. Provision of chemical drugs in the long term have side effects. Shallots (Allium ascalonicum L.) contain flavonoids which can inhibit the xanthine oxidase enzyme, so as to reduce uric acid levels. Red ginger extract (Zingiber officinale var rubrum) is rich in gingerol to reduce uric acid with anti-inflammatory effect. Polyberbal combination is expected to increase the effectiveness of therapy. The research aims to determine the effectiveness of polyherbal combinations. 25 male rats (2-3 months) were divided into five groups, namely negative control, positive control, (P1) 25% red ginger extract: 75% shallot, (P2) 50% red ginger extract: 50% shallot, ( P3) 75% red ginger extract: 25% shallot. Induction of potassium oxonate 250 mg / kg BW intraperitoneally on the 7th day. The first measurement of uric acid on the 14th day with Urid Acid Toos, the 15th to 28th days was administered herbal / chemical drugs according to the group. Measurement of second and third uric acid on days 21 and 28. Data were analyzed by one-way Anova (RAL), further significance tests using Least Significant Different (LSD). The results showed that administration of potassium oxonate and combination of herbal extracts had a significant effect (p <0.05) on uric acid levels in all groups. The most effective herbal combination doses are (P3) 75% red ginger (450 mg / 200 gr) and 25% shallot (150 mg / 200 gr BW). The conclusion of this study is the combination of red ginger extract and shallot can be recommended as a uric acid-lowering agent.
Keracunan coklat pada anjing: manajemen terapi dan pencegahan Yanuartono Yanuartono; Alfarisa - Nururrozi; Soedarmanto - Indarjulianto; Slamet - Raharjo; Hary - Purnamaningsih; Nurman - Haribowo
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.51559

Abstract

Chocolate poisoning has long been recognized as a common cause mostly in dogs, although many species are susceptible. Contributing factors include indiscriminate eating habits and readily available sources of chocolate. In general, the poisoning resulted from a lack of public knowledge of the health hazard to dogs that may be imposed by these products.Chocolate is derived from the seeds of the plant Theobroma cacao, and the main toxic components are the methylxanthine alkaloids theobromine and caffeine, causing gastrointestinal, cardiovascular and central nervous signs. Diagnosis is based on history of exposure, along with clinical signs. Amphetamine or cocaine toxicosis, and ingestion of antihistamines, antidepressants, or other CNS stimulants should be considered in the differential diagnosis. Stabilization of symptomatic animals is a priority in treating chocolate toxicosis. Although there is no specific antidote, supportive management includes induction of vomiting and administration of activated charcoal, oxygen, and intravenous fluids. Preventing exposure is the key to reducing the incidence of these poisoning episodes. Therefore, it is important to increase the knowledge of dogs owners with regard to foodstuffs that must not be fed to dogs and should be stored outside their reach.
Gonadotropine Releasing Hormone (GnRH) Receptor Profile from Cow’s Hypothalamus Irma Dian Nurani; Claude Mona Airin; Pudji Astuti; Khrisdiana Putri; Bambang Sutrisno
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.51882

Abstract

Gonadotropine Releasing Hormone (GnRH) is one of the recommended hormones to overcome ovulation problems and it can increase pregnancy rate so that it is used in government programs to increase cattle population in Indonesia, although the results are not yet optimal. The purpose of this study is to compare the composition of bovine GnRH receptor nucleotides with the GenBank reference so that the level of genetic diversity of bovine receptors in Indonesia is known. PCR product sequencing using Promoter F and Exon 1 R primers were further aligned with the reference sequences of Bos Taurus GnRHR mRNA GenBank using the MEGA X program. The results of the analysis found the presence of Single Nucleotide Polymorphism (SNP) in the coding region  of 1st cow  position 38(A> T), 261(C > T), 342(C >T), 411(C >T) and 495(C > T) and 2nd cow positions 261(C >T). Changes in amino acids were also detected in 1st cow position 13 (H> L). The presence of SNP was found to indicate genomic variation between individuals at cattle receptors in Indonesia.
Deteksi Staphylococcus aureus dan Staphylococcus sp. Secara Langsung Dari Susu Segar Kambing Peranakan Etawa dengan Teknik PCR Fatkhanuddin Aziz; Fajar Budi Lestari; Sarah Nuraida S; Endah Purwati; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.53802

Abstract

Genus Staphylococcus merupakan salah satu patogen bakteri penyebab mastitis yang menyebabkan kerugian ekonomi pada kambing Peranakan Etawa. Diantara Staphylococcus sp. yang dapat tumbuh dengan baik dalam susu segar, diketahui Staphylococcus aureus dapat membahayakan kesehatan manusia yang mengkonsumsi (food borne disease) karena kemampuannya dalam memproduksi enterotoksin yang tahan terhadap enzim pencernaan maupun pemanasan. Tujuan penelitian ini adalah mendeteksi Staphylococcus sp. dan S. aureus secara langsung dari susu kambing peranakan etawa dengan teknik PCR.Metode yang dilakukan adalah mengekstraksi DNA dari 60 sample susu segar dengan prinsip spin column-based nucleic acid purification dan kemudian dilakukan amplifikasi gen spesifik 23S rRNA Staphylococcus sp. dan S. aureus. Hasil PCR diketahui 37 (61%) sampel susu positif mengandung Staphylococcus sp. dan hanya 1 (1,6%) sampel mengandung S. aureus. Metode deteksi dengan PCR dapat digunakan untuk mendeteksi kontaminan Staphylococcus sp. dan S. aureus dengan waktu yang singkat

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