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Jurnal Kedokteran Hewan
Published by Universitas Syiah Kuala
ISSN : 1978225X     EISSN : 25025600     DOI : 10.21157
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
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Search results for , issue "Vol 10, No 2 (2016): September" : 44 Documents clear
PEMBERIAN PASAK BUMI MEMENGARUHI KADAR TESTOSTERON DAN SPERMATOZOA KAMBING PERANAKAN ETAWA (The Administration of Eurycoma longifolia Jack Affect the Level of Testosterone and Spermatozoa of Etawa Crossbreed Goat) Hurip Pratomo; Yudi Yudi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.384 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5046

Abstract

ABSTRACT The study aimed to find out the effect of Eurycoma longifolia Jack on testosterone level and spermatozoa quality of Etawa crossbreed goat. Goats were divided into two treatment groups, three goats each. Group 1 (K1) was control group which given orally 20 ml of distilled water every morning at 9:00 am for six days, and group 2 (K2) was administered with Eurycoma longifolia with the dose of 90 mg/kg bw in 20 ml of distilled water orally every morning at 9:00 am for six days. The concentration of testosterone was measured on day 1, 3, and 6 using the method of enzyme-linked immunosorbent assay (ELISA). The quality of sperm consisted of concentration and percentage of life sperm. The results showed that level of testosterone on K2 increased started from day 1 (9.36 ng/ml) to day 6 (12.43 ng/ml) (P0.05). The percentage of life spermatozoa increased higher on K2 compare to K1 on day 3 to day 6, that was 88.6 and 89.8% on K1 to 91.0 and 92.7% on K2. In conclusion, the administration of pasak bumi with the dose of 90 mg/kg bw in 20 ml of distilled water for 6 days able to increase testosterone levels and percentage of life sperm of Etawa crossbreed goat.
PENGGANTIAN BOVINE SERUM ALBUMIN PADA PENGENCER CEP-2 DENGAN SERUM DARAH SAPI DAN PUTIH TELUR TERHADAP KUALITAS SEMEN CAIR SAPI LIMOUSIN SELAMA PENDINGINAN (The Substitution of Bovine Serum Albumin with Cattle Blood Serum and Egg White in CEP-2 Diluent on the Quality of Limousin Bull Liquid Semen during Refrigerating) Trinil Susilawati; Feri Eka Wahyudi; Inna Anggraeni; Nurul Isnaini; Muhammad Nur Ihsan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.844 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5025

Abstract

This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.
PEMBERIAN PASAK BUMI MEMENGARUHI KADAR TESTOSTERON DAN SPERMATOZOA KAMBING PERANAKAN ETAWA (The Administration of Eurycoma longifolia Jack Affect the Level of Testosterone and Spermatozoa of Etawa Crossbreed Goat) Pratomo, Hurip; Yudi, Yudi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5046

Abstract

ABSTRACT The study aimed to find out the effect of Eurycoma longifolia Jack on testosterone level and spermatozoa quality of Etawa crossbreed goat. Goats were divided into two treatment groups, three goats each. Group 1 (K1) was control group which given orally 20 ml of distilled water every morning at 9:00 am for six days, and group 2 (K2) was administered with Eurycoma longifolia with the dose of 90 mg/kg bw in 20 ml of distilled water orally every morning at 9:00 am for six days. The concentration of testosterone was measured on day 1, 3, and 6 using the method of enzyme-linked immunosorbent assay (ELISA). The quality of sperm consisted of concentration and percentage of life sperm. The results showed that level of testosterone on K2 increased started from day 1 (9.36 ng/ml) to day 6 (12.43 ng/ml) (P0.05). The percentage of life spermatozoa increased higher on K2 compare to K1 on day 3 to day 6, that was 88.6 and 89.8% on K1 to 91.0 and 92.7% on K2. In conclusion, the administration of pasak bumi with the dose of 90 mg/kg bw in 20 ml of distilled water for 6 days able to increase testosterone levels and percentage of life sperm of Etawa crossbreed goat.
PENGGANTIAN BOVINE SERUM ALBUMIN PADA PENGENCER CEP-2 DENGAN SERUM DARAH SAPI DAN PUTIH TELUR TERHADAP KUALITAS SEMEN CAIR SAPI LIMOUSIN SELAMA PENDINGINAN (The Substitution of Bovine Serum Albumin with Cattle Blood Serum and Egg White in CEP-2 Diluent on the Quality of Limousin Bull Liquid Semen during Refrigerating) Susilawati, Trinil; Wahyudi, Feri Eka; Anggraeni, Inna; Isnaini, Nurul; Ihsan, Muhammad Nur
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5025

Abstract

This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.505.90, 3636.16, 34.006.58, 40.503.69, 38.503.37, and 38.504.12, respectively, while the percentage of sperms viability were 75.164.30, 70.502.88, 73.802.80, 74.803.30, 75.133.13, and 74.034.13, respectively, and the percentage of sperms abnormality were 10.142.34, 10.621.34, 11.332.00, 10.942.82, 10.021.95, and 10.781.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.505.90, 3636.16, 34.006.58, 40.503.69, 38.503.37, and 38.504.12, respectively, while the percentage of sperms viability were 75.164.30, 70.502.88, 73.802.80, 74.803.30, 75.133.13, and 74.034.13, respectively, and the percentage of sperms abnormality were 10.142.34, 10.621.34, 11.332.00, 10.942.82, 10.021.95, and 10.781.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.
PENENTUAN KONSENTRASI SODIUM DODECYL SULFATE DALAM PENGENCER RINGER LAKTAT-KUNING TELUR UNTUK PRESERVASI SEMEN AYAM PELUNG (Determination of Sodium Dodecyl Sulfate Concentration in Ringer Lactate-Egg Yolk Extender for Pelung Rooster Semen Preservation) Hidayat, Numan; Sumantri, Cece; Afnan, Rudi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5091

Abstract

The objective of this experiment was to determine the addition of sodium dodecyl sulfate (SDS) to Ringer Lactate-Egg Yolk (RL-EY) extender on pelung chicken semen preservation. Semen was collected three times a week from three pelung chickens. Collected semen was evaluated macroscopically and microscopically. Only ejaculates of at least 70% sperm motility was then divided into three equal microtubes. Each of them diluted with RL-EY with ratio 90%:10%, then added with 0.00, 0.025, and 0.05% SDS respectively. The liquid semen then stored at 5 C for 72 hours. Sperm motility and viability were observed every 12 hours. The addition of 0.025% SDS showed higher spermatozoa motility and viability (72.081.44% and 80.821.30%) which were significantly higher (P0.05) than 0.00 and 0.05% SDS addition at 24 hours of storage. There was no differences on the spermatozoa motility and viability between 0.00 and 0.05% SDS addition. The decrease of spermatozoa motility and viability was observed in 0.025% SDS addition (4.170.56% and 4.650.59%, respectively) that significantly lower compared to 0% and 0.05% SDS addition at 24 hours of storage. In conclusion, 0.025% SDS addition in a Ringer Lactate -Egg Yolk extender maintained pelung spermatozoa motility and viability better than 0.00 and 5% SDS addition.
PENGARUH PROBIOTIK TERHADAP KOLESTEROL DARAH PADA AYAM PETELUR (LAYER) (Effect of Probiotik on Blood Cholesterol in Laying Hens) Sumardi, Sumardi; Sutyarso, Sutyarso; Susanto, Gregorius Nugroho; Kurtini, Tintin; Hartono, Madi; Puspitaningsih N.W., Rr. Etty
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5042

Abstract

The aim of this research was to find out the effect of probiotic on blood cholesterol in laying hens. Materials used were laying hens (Isa Brown strain) 43 weeks old and ration using probiotics. This research was conducted by using completely randomized design (CRD). The advanced research was done with testing the local probiotic with various concentration treatment, they are P0 (ration without probiotic), P1 (ration + 1% local probiotic), P2 (ration + 2% local probiotic), and P3 (ration + 3% local probiotic) with 5 time repetitions and in every repetitions consists of 2 chicken. Variables that were observed in the advance research were laying henss blood cholesterol that consists of total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), triglycerides, and total leucocyte. The level of total cholesterol on P0, P1, P2, and P3 were 129.0038.26, 116.00 26.53, 121.0033.72, and 137.0052.53 mg/dL (P0.05) respectively; the HDL level were 50.6016.47, 35.8011.28, 59.4019.74; 56.8030.56 mg/dL (P0.05); the LDL level were 100.2049.53, 39.2017.14, 104.6051.23, and 96.8066.22 mg/dL (P0.05), respectively; the trigycerides level were 862.00355.5, 533.00146.1, 829.00242.6, and 810.00304 mg/dL (P0.05), respectively; and leucocyte count were 22.001.72, 21.003.79, 31.003.99, and 34.003.55 x103 mm3 (P0.01), respectively. In conclusion, the administrtion of local probiotic do not effect the blood cholesterol of layer chicken.
PREVALENSI DAN FAKTOR RISIKO KOKSIDIOSIS PADA SAPI PERAH DI KABUPATEN BANDUNG (Prevalence and Risk Factor of Coccidiosis in Dairy Cattle in Bandung District) Sufi, Isrok Malikus; Cahyaningsih, Umi; Sudarnika, Etih
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5138

Abstract

This study aimed to determine the prevalence and associated risk factors of coccidiosis. Samples were obtained from 400 dairy cattle (196 calves aged 6 months, 37 calves aged 6-12 months, and 167 calves aged 12 months). Feces samples were collected, examined and counted for prevalence and number of oocyst per gram faeces (OPG) by McMaster technique. A questionnaire was design to record information about animal health and husbandry, individually. Risk factors associated with the prevalence of Eimeria in cattle were analyzed by logistic regression model. The overall prevalence and the average of OPG of Eimeria in cattle was 179 (44.75%) and 286.75, while highest prevalence of Eimeria was observed in calves aged less than 6 months. Cattle aged more than 12 months showed significantly different relationship (P0.05) to the prevalence of Eimeria infection compare to calves aged less than 6 months and aged 6-12 months. The presence of an immature immune system in younger calves resulting in their higher susceptibility to coccidiosis. Among management and animal health practices, floor type and treatment of cattle influence the prevalence of Eimeria in cattle.
STUDI ANATOMIS DAN HISTOLOGIS PANKREAS BIAWAK AIR (Varanus salvator) (Anatomical and Histological Study of the Pancreas of Water Monitor Lizard (Varanus salvator)) Hamny, Hamny; Iqbal, Muhammad; Wahyuni, Sri; Sabri, Mustafa; Jalaluddin, M.; Rinidar, Rinidar
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5047

Abstract

The aim of this research was to determine the anatomy and histology of pancreas of water monitor lizard (Varanus salvator). Two pancreas were used in this research (a male and a female). Water monitor lizard was euthanized using chloroform and subsequently necropsied to collect the pancreas. The pancreas was washed in 0.9 % NaCl solution. Anatomical observations were carried out including location, shape, and color of pancreas, also the length and weight measurements. Then, pancreas was fixed with 4% paraformaldehyde for 7 days to proceed to histological preparation and hematoxylin eosin (HE) staining. The results of the anatomical observations showed that pancreas of water monitor lizard have two lobes, dorsal lobe (splenic lobe) and ventral lobe (duodenal lobe). Dorsal lobe which was in oval form adhered with the spleen, and ventral lobe was located in the cranial of intestines with the form of long spherical. Both of pancreas lobes were white-yellow color with the length of 1.450.64 cm (dorsal lobe) and 7.000.42 cm (ventral lobe). Weight of dorsal lobe was 0.150.01 g and ventral lobe was 6.352.30 g. The histological observations found the acinar cells, ducts, and blood vessels in both of pancreas lobes. Additionally, the similar structure of Langerhans islet only found at the ventral lobe of pancreas. In conclusion, pancreas of water monitor lizard consists of two lobes which located differently. Ventral lobe is larger than the dorsal lobe. Both lobes have similar histological structure except the Langerhans islets are only appeared in the ventral lobe.
ANATOMI DAN GAMBARAN ULTRASOUND ORGAN REPRODUKSI SELAMA SIKLUS ESTRUS PADA KUDA GAYO BETINA (Anatomy and Ultrasound Imaging of Reproductive Organs of Gayo Mares During Estrous Cycle) Melia, Juli; Agil, Muhammad; Supriatna, Iman; Amrozi, Amrozi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5026

Abstract

The present study examines anatomy of Gayo mare reproductive organs. This study used three sample of Gayo mare reproductive organs (n= 3) for observation of morphology and morphometric of the mare reproductive organs. The ovarium was fixed in 4% paraformaldehyde solution then followed by histological method and stained using hematoxylin and eosin (HE) and Massons trichome (MT). Three mares were observed for diameter and changes overview of uterus during estrous cycle in real time using ultrasound. The results showed that, in general, the anatomy of Gayo mares reproductive organs was similar to other mares, but smaller in morphometry. The total length of the Gayo mares reproductive tract from labia to apex cornua was 48.001.00 cm. Weight of Gayo mares left ovary was 19.077.70 g and the right was 24.430.83 g. Histologically, there was no difference between Gayo mares structure and other mares. In cortex uteri there were some follicles surrounded by capillary, various development stages of follicles, healthy follicles, atretic follicle, and corpus albican; while in medulla there were a lot of connective tissues. Ultrasound of the uterus showed the change in diameter during estrous cycle with the largest diameter of corpus uteri was 4.430.10 cm in horses with estrous cycle of 21 days and 6.300.93 cm in horses with 24 days estrous cycle. In conclusion, the morphometry of Gayo mare reproductive organs are smaller than the other horses and there are differences in diameter of the uterus during the estrous cycle due to the changes of endometrium thickness.
PENGARUH PEMBERIAN EKSTRAK BUAH MENGKUDU (Morinda citrifolia) DALAM LARUTAN NATRIUM KLORIDA FISIOLOGIS SEBAGAI BAHAN PENGENCER SEMEN TERHADAP PENINGKATAN KUALITAS SPERMATOZOA AYAM BURAS PADA SUHU RUANG (Effect of Noni Extract Fruit (Morinda citrifolia) in Physiological Saline Solution as Diluent on the Native Chicken Spermatozoa Quality at Room Temperature) Murcahyana, Murcahyana; Susilawati, Trinil; Isnaini, Nurul
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5092

Abstract

The purpose of this research was to analyse the quality and storability of native chicken semen at room temperature after diluted with physiological saline solution (NaCl) supplemented with noni fruit extract. Research methodology used was nested randomized design which variables observed covering the quality of chicken spermatozoa macroscopically and microscopically with the doses of noni fruit extract of 0 % (P0), 10 % (P1), 20 % (P2), and 30 % (P3). Rate of spermatozoa motility at 0, 1, 2, and 3 hours on P0 were 83.704.74, 63.309.63, 32.908.52, and 15.006.20; P1 were 85.305.44, 72.7010.06, 51.9011.75, and 28.505.68; P2 were 84.606.40, 78.508.59, 57.9010.73, and 33.709.06; and P3 were 81.807.30, 64.207.93, 40.3011.66, and 19.807.47, respectively. Rate of spermatozoa viability at 0, 1, 2, and 3 hours on P0 were 85.309.09, 65.106.15, 32.1011.86, and 10.309.09; P1 wer 87.406.22, 72.705.33, 50.8013.59, and 26.5010.99; P2 were 68.585.30, 77.704.79, 56.3013.76, and 32.7013.79; P3 were 81.208.04, 68.7010.40, 36.2016.61, and 16.9011.93, respectively. Rate of spermatozoa abnormality at 0, 1, 2, and 3 hours on P0 were 8.703.40, 12.204.42, 17.805.67, and 20.306.38; P1 were 7.802.04, 9.802.69, 13.604.45, and 16.505.19; P2 wer 8.404.33, 10.002.45, 12.505.21, and 15.803.71; and P3 were 9.603.41, 10.902.64, 17.105.61, and 21.208.16, respectively. It can be concluded that the addition of noni fruit extract at the dose of 10 % and 20 % in physiological saline solutin maintain the native chicken spermatozoa quality up to 2 hours post storage at room temperature.

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