cover
Contact Name
Tongku Nizwan Siregar
Contact Email
jkh@usk.ac.id
Phone
+626517551536
Journal Mail Official
jkh@usk.ac.id
Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia
Location
Kab. aceh besar,
Aceh
INDONESIA
Jurnal Kedokteran Hewan
ISSN : 1978225X     EISSN : 25025600     DOI : 10.21157
Core Subject : Health, Science,
Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.
Articles 10 Documents
Search results for , issue "Vol 14, No 2 (2020): June" : 10 Documents clear
KOSAKONIA SP. PROTEOLYTIC BACTERIA ISOLATED FROM RUMEN AND RETICULUM OF ACEH CATTLE Safika Safika; Wenny Novita Sari; Gressha Vionalle Ademi; Ulfi Widi Arsih; Darmawi Darmawi
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (382.339 KB) | DOI: 10.21157/j.ked.hewan.v14i2.16426

Abstract

The aim of this study was to identify proteolytic bacteria from the ruminal and reticulum fluids of aceh cattle based on the 16S rRNA gene. Samples used were ruminal and reticulum fluids of aceh cattle slaughtered in Abattoir of Aceh Besar. Samples were diluted and cultured into Skim Milk Agar medium at 39 C for 48 hours. The morphology of bacterial colonies growth in the medium was observed. Colonies resulted in the largest clear zone were isolated and used for Deoxyribonucleic Acid (DNA) isolation, 16S rRNA gene amplification and sequencing. Theresults showed that morphology of dominant colonies was yellowish white color, round shape, position on the agar surface. The results ofphylogenetic analysis of RS1 and ReS2 isolates isolated from rumen and reticulum fluids of aceh cattle respectively had a close familialrelationship and belonged to the bacterial group of Kosakonia. Sequence homology showed isolate RS1 and ReS2 are probably either newEnterobacteriaceae species or unconfirmed species. Halo zone produced by ruminal bacteria had a wider diameter (25 mm vs 20 mm) than thatcaused by reticulum bacteria). Based on the results, RS1 (bacterium in the rumen) and ReS2 (bacterium in the reticulum) belong to similar type, namely Kosakonia sp. with a proteolytic activity. Presumably, these bacteria originate from the rumen that enters the reticulum with degraded feed.
EVALUATION OF SULFURIC ACID, BARIUM CHLORIDE, AND SEED GERMINATION ASSAY METHODS AS EARLY PREGNANCY DETECTION INSTRUMENTS IN CATTLE Zul Azmi; Muhammad I. Desem; Hastuti Handayani S. Purba; Dwi Endrawati; Faidah Rachmawati; Eni Kusumaningtyas; Didik T. Subekti
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.717 KB) | DOI: 10.21157/j.ked.hewan.v14i2.15271

Abstract

This study aims to validate and re-evaluate the use of H2SO4, BaCl2, and seed germination assay (SGA) methods to detect early pregnancy in cattle. Validation was carried out in order to find out the actual test performance value, which include accuracy, sensitivity, and specificity.Validation test was done by reacting the urine of pregnant and non-pregnant cattle populations with H2SO4 solution diluted at a ratio of 1 : 4, with 1% BaCl2 solution or through germination of mung bean seeds in Petri dishes. The results of this study indicated that the H2SO4 and BaCl2 methods have poor accuracy (21.88% and 28.13%) with the sensitivity of 81.82% and 100% and specificity of 9.43% and 13.21%, respectively. Meanwhile, the SGA method had a fairly good accuracy (74.55%) with sensitivity and specificity of 45.46% and 81.82%, respectively. Based on these results, the H2SO4, BaCl2, and SGA methods are not recommended for detection of early pregnancy in the field.
DETECTION OF SERUM MACRO MINERALS CONCENTRATION IN REPEAT BREEDING ACEH CATTLE Cut Nila Thasmi; Husnurrizal Husnurrizal; Muslim Akmal; Sri Wahyuni; Tongku Nizwan Siregar
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.373 KB) | DOI: 10.21157/j.ked.hewan.v14i2.16543

Abstract

The purpose of this study was to determine the macro minerals concentration in serum of repeat breeding (RB) Aceh cattle. In this study 16 Aceh cattle were examined; they consisted of 7 fertile Aceh cattle (P1) and 9 Aceh cattle with RB (P2), all of which were 3-8 years old with abody condition score (BCS) of 3-4. Serum collection was carried out for examining the level of serum minerals, including magnesium, phosphorus, sodium, potassium, chloride, and calcium. Data was analysed using T-test. The levels of magnesium, phosphorus, sodium,potassium, chloride, and calcium in P1 vs P2 were 2.18±0.60 vs. 2.20±0.34 mg/dL; 6.18±1.34 vs. 6.48±0.74 mg/dL; 142.71±5.09 vs.142.44±2.29 mmol/L; 4.81±0.76 vs. 4.76±0.51 mmol/L; 104.57±4.35 vs. 107.67±7.36 mmol/L; and 9.07±0.45 vs. 9.90 ± 0.60 mg/dL (P0.05), respectively. It was concluded that the concentration of serum macro minerals do not affect the incidence of RB in Aceh cattle.
ANTIBACTERIAL ACTIVITY AND HEMATOLOGICAL PROFILE OF RAT (Rattus norvegicus) DUE TO ADMINISTRATION OF ETHANOL EXTRACT OF SOURSOP FLOWERS (Annona muricata L.) AND Salmonella enteritidis INFECTION Zuraidawati Zuraidawati; Sugito Sugito; Darmawi Darmawi; Taufiq Taufiq
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.221 KB) | DOI: 10.21157/j.ked.hewan.v14i2.11646

Abstract

This study aimed to determine the antibacterial activity of soursop flower extract (Annona muricata L.) and hematological profile of rats (Rattus norvegicus) due to administration of soursop flower ethanol extract and Salmonella enteritidis infection. The concentrations of soursop flower ethanol extract used for the antibacterial activity test were 25%, 50%, 75%, 100%, with the antibiotic ampicillin 10 μg/disk was used s a positive control (PC) and dimethyl sulfoxide (DMSO) 10% as a negative control (NC). For examination of hematological features, 15 male rat aged two months were used. All rats were divided into 5 treatment groups, each consisting of three rats. The NC group was not given soursop flower ethanol extract and S. enteritidis infection. The PC group was not given soursop flower extract but was given S. enteritidis infection. Groups P1, P2, and P3 were given ethanol extracts of soursop flower at a dose of 0.18, 0.36, and 0.72 g/rat/day peroral for a week using gastric sonde. On the following day (after administration of soursop flower extract) the first blood drawing was performed. All rats, except NC group, were then infected with S. enteritidis intraperitonially at dose of 3x108 CFU/mL dose (0.5 mL McFarland). One week after being infected with S. enteritidis, a second blood drawing was performed. The results of the antibacterial activity test showed that there was no antibacterial activity was observed since no inhibition at various concentrations was formed. The administration of soursop flower extract at various dosage levels was able to maintain the number of leukocytes but reduced the number of erythrocytes, hemoglobin concentration, hematocrit value and the number of platelets in rats; whereas S. enteritidis infection decreased all the hematologic features of lab rats.
FEEDING LOCAL FERMENTED WITH EM-4, AMMONIUM SULFATE, AND UREA ON THE BLOOD FAT LEVEL OF MALE DUCK Dian Septinova; Aisyah Yuli Arti; Rudy Sutrisna; Syahrio Tantalo; Madi Hartono
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.898 KB) | DOI: 10.21157/j.ked.hewan.v14i2.16501

Abstract

The objective of this study was to determine the effect of commercial ration by the addition of ammonium sulfate and urea fermented feed ingredients on the blood fat levels of local male ducks. This study used a completely randomized design with 32 male ducks which weredivided into four groups namely R0, R1, R2, R3, and R4. Each group was given the following treatment: R0 only given commercial ration;R1 was given 80% commercial ration + 20% fermentation of EM-4 + 1.5% urea); R2 was given 80% commercial ration + 20% EM-4 + 1.5%ammonium sulfate fermentation; and R3 was given 80% commercial ration + 20% EM-4 + 1.5% urea + 1.5% ammonium sulfate. Variablesin this study were blood fat consisting of cholesterol, triglyceride, High-Density Lipoprotein (HDL), and Low-Density Lipoproteins (LDL).The data obtained were analyzed by using 5% real level and LSD. The results showed that the provision of commercial ration added withlocal feed fermented with ammonium sulfate and urea had a significant effect on cholesterol levels (P0.5), but not significant (P0.05) ontriglyceride, HDL, and LDL levels. Furthermore, the ration that gave the best effect on the blood fat levels of male ducks blood was the ration given to R3 group.
KOSAKONIA SP. PROTEOLYTIC BACTERIA ISOLATED FROM RUMEN AND RETICULUM OF ACEH CATTLE Safika, Safika; Sari, Wenny Novita; Ademi, Gressha Vionalle; Arsih, Ulfi Widi; Darmawi, Darmawi
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.16426

Abstract

The aim of this study was to identify proteolytic bacteria from the ruminal and reticulum fluids of aceh cattle based on the 16S rRNA gene. Samples used were ruminal and reticulum fluids of aceh cattle slaughtered in Abattoir of Aceh Besar. Samples were diluted and cultured into Skim Milk Agar medium at 39 C for 48 hours. The morphology of bacterial colonies growth in the medium was observed. Colonies resulted in the largest clear zone were isolated and used for Deoxyribonucleic Acid (DNA) isolation, 16S rRNA gene amplification and sequencing. Theresults showed that morphology of dominant colonies was yellowish white color, round shape, position on the agar surface. The results ofphylogenetic analysis of RS1 and ReS2 isolates isolated from rumen and reticulum fluids of aceh cattle respectively had a close familialrelationship and belonged to the bacterial group of Kosakonia. Sequence homology showed isolate RS1 and ReS2 are probably either newEnterobacteriaceae species or unconfirmed species. Halo zone produced by ruminal bacteria had a wider diameter (25 mm vs 20 mm) than thatcaused by reticulum bacteria). Based on the results, RS1 (bacterium in the rumen) and ReS2 (bacterium in the reticulum) belong to similar type, namely Kosakonia sp. with a proteolytic activity. Presumably, these bacteria originate from the rumen that enters the reticulum with degraded feed.
EVALUATION OF SULFURIC ACID, BARIUM CHLORIDE, AND SEED GERMINATION ASSAY METHODS AS EARLY PREGNANCY DETECTION INSTRUMENTS IN CATTLE Azmi, Zul; Desem, Muhammad I.; S. Purba, Hastuti Handayani; Endrawati, Dwi; Rachmawati, Faidah; Kusumaningtyas, Eni; Subekti, Didik T.
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.15271

Abstract

This study aims to validate and re-evaluate the use of H2SO4, BaCl2, and seed germination assay (SGA) methods to detect early pregnancy in cattle. Validation was carried out in order to find out the actual test performance value, which include accuracy, sensitivity, and specificity.Validation test was done by reacting the urine of pregnant and non-pregnant cattle populations with H2SO4 solution diluted at a ratio of 1 : 4, with 1% BaCl2 solution or through germination of mung bean seeds in Petri dishes. The results of this study indicated that the H2SO4 and BaCl2 methods have poor accuracy (21.88% and 28.13%) with the sensitivity of 81.82% and 100% and specificity of 9.43% and 13.21%, respectively. Meanwhile, the SGA method had a fairly good accuracy (74.55%) with sensitivity and specificity of 45.46% and 81.82%, respectively. Based on these results, the H2SO4, BaCl2, and SGA methods are not recommended for detection of early pregnancy in the field.
DETECTION OF SERUM MACRO MINERALS CONCENTRATION IN REPEAT BREEDING ACEH CATTLE Thasmi, Cut Nila; Husnurrizal, Husnurrizal; Akmal, Muslim; Wahyuni, Sri; Siregar, Tongku Nizwan
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.16543

Abstract

The purpose of this study was to determine the macro minerals concentration in serum of repeat breeding (RB) Aceh cattle. In this study 16 Aceh cattle were examined; they consisted of 7 fertile Aceh cattle (P1) and 9 Aceh cattle with RB (P2), all of which were 3-8 years old with abody condition score (BCS) of 3-4. Serum collection was carried out for examining the level of serum minerals, including magnesium, phosphorus, sodium, potassium, chloride, and calcium. Data was analysed using T-test. The levels of magnesium, phosphorus, sodium,potassium, chloride, and calcium in P1 vs P2 were 2.180.60 vs. 2.200.34 mg/dL; 6.181.34 vs. 6.480.74 mg/dL; 142.715.09 vs.142.442.29 mmol/L; 4.810.76 vs. 4.760.51 mmol/L; 104.574.35 vs. 107.677.36 mmol/L; and 9.070.45 vs. 9.90 0.60 mg/dL (P0.05), respectively. It was concluded that the concentration of serum macro minerals do not affect the incidence of RB in Aceh cattle.
ANTIBACTERIAL ACTIVITY AND HEMATOLOGICAL PROFILE OF RAT (Rattus norvegicus) DUE TO ADMINISTRATION OF ETHANOL EXTRACT OF SOURSOP FLOWERS (Annona muricata L.) AND Salmonella enteritidis INFECTION Zuraidawati, Zuraidawati; Sugito, Sugito; Darmawi, Darmawi; Taufiq, Taufiq
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.11646

Abstract

This study aimed to determine the antibacterial activity of soursop flower extract (Annona muricata L.) and hematological profile of rats (Rattus norvegicus) due to administration of soursop flower ethanol extract and Salmonella enteritidis infection. The concentrations of soursop flower ethanol extract used for the antibacterial activity test were 25%, 50%, 75%, 100%, with the antibiotic ampicillin 10 g/disk was used s a positive control (PC) and dimethyl sulfoxide (DMSO) 10% as a negative control (NC). For examination of hematological features, 15 male rat aged two months were used. All rats were divided into 5 treatment groups, each consisting of three rats. The NC group was not given soursop flower ethanol extract and S. enteritidis infection. The PC group was not given soursop flower extract but was given S. enteritidis infection. Groups P1, P2, and P3 were given ethanol extracts of soursop flower at a dose of 0.18, 0.36, and 0.72 g/rat/day peroral for a week using gastric sonde. On the following day (after administration of soursop flower extract) the first blood drawing was performed. All rats, except NC group, were then infected with S. enteritidis intraperitonially at dose of 3x108 CFU/mL dose (0.5 mL McFarland). One week after being infected with S. enteritidis, a second blood drawing was performed. The results of the antibacterial activity test showed that there was no antibacterial activity was observed since no inhibition at various concentrations was formed. The administration of soursop flower extract at various dosage levels was able to maintain the number of leukocytes but reduced the number of erythrocytes, hemoglobin concentration, hematocrit value and the number of platelets in rats; whereas S. enteritidis infection decreased all the hematologic features of lab rats.
FEEDING LOCAL FERMENTED WITH EM-4, AMMONIUM SULFATE, AND UREA ON THE BLOOD FAT LEVEL OF MALE DUCK Septinova, Dian; Arti, Aisyah Yuli; Sutrisna, Rudy; Tantalo, Syahrio; Hartono, Madi
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.16501

Abstract

The objective of this study was to determine the effect of commercial ration by the addition of ammonium sulfate and urea fermented feed ingredients on the blood fat levels of local male ducks. This study used a completely randomized design with 32 male ducks which weredivided into four groups namely R0, R1, R2, R3, and R4. Each group was given the following treatment: R0 only given commercial ration;R1 was given 80% commercial ration + 20% fermentation of EM-4 + 1.5% urea); R2 was given 80% commercial ration + 20% EM-4 + 1.5%ammonium sulfate fermentation; and R3 was given 80% commercial ration + 20% EM-4 + 1.5% urea + 1.5% ammonium sulfate. Variablesin this study were blood fat consisting of cholesterol, triglyceride, High-Density Lipoprotein (HDL), and Low-Density Lipoproteins (LDL).The data obtained were analyzed by using 5% real level and LSD. The results showed that the provision of commercial ration added withlocal feed fermented with ammonium sulfate and urea had a significant effect on cholesterol levels (P0.5), but not significant (P0.05) ontriglyceride, HDL, and LDL levels. Furthermore, the ration that gave the best effect on the blood fat levels of male ducks blood was the ration given to R3 group.

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