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Ayyub Harly Nurung
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Editor's Address: 3rd Floor Laboratory Faculty of Pharmacy, 2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia
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Journal Microbiology Science
Published by Universitas Muslim Indonesia
ISSN : 2808392X     EISSN : 28083911     DOI : https://doi.org/10.56711/jms
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Journal Microbiology Sciences (J. Mic. Sciences) receives writing in the form of research results, scientific studies and brief descriptions relating to Microbiology, such as Molecular and Microbial Epidemiology Clinical Microbiology Medical Microbiology Pharmaceutical Biotechnology and others related to Microbiology Sciencee
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 15 Documents
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Search results for , issue "Vol 4, No 1 (2024)" : 15 Documents clear
Antibacterial Activity of Endophytic Fungi Isolated from Bidara Roots (Ziziphus mauritiana Lam.) Against Bacteria That Cause Skin Infections Using TLC-Bioutography Sari, Andini Indah Permata; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.996

Abstract

One common disease found in tropical countries is skin infection. Inappropriate use of antibiotics to treat skin infections can lead to bacterial resistance, making it necessary to explore the source of bioactive compounds in natural materials. The Bidara plant (Ziziphus mauritiana Lam.) is known to have antibacterial properties. This research aimed to determine the antibacterial activity of endophytic fungal isolated from the parts of the Bidara plant against bacteria that cause skin infections using the TLC-Bioautography method. In this research, the isolate with the code IFAZ-6 was purified to obtain a pure endophytic fungal isolate, and macroscopic and microscopic examinations were conducted on that isolate. The obtained isolate was fermented for 21 days at room temperature using MYB (Maltose Yeast Broth) medium. The fermentation products were extracted using ethyl acetate (1:1). The obtained extract was further tested using TLC with a chloroform: methanol eluent (7:1). The TLC-Bioautography results showed Rf values of 0.81 and 0.58 indicating antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Propionibacterium acnes bacteria. The identification of chemical components in the endophytic fungal isolate from the Bidara plant confirmed the presence of flavonoids, alkaloids, and anthraquinones. The extract from the endophytic fungal isolate with the code IFAZ-6 indicated antibacterial activity.
Free Antiradical Activity Test of Endophytic Fungi Isolates of Kasumba Turate (Carthamus tinctorius (L) Leaves from Galesong Takalar with KLT-Autography Method Sari, Dewi Mustika; Kosman, Rachmat; Rusli, Rusli
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1027

Abstract

The kasumba turate plant (Carthamus tinctorius (L.) has the potential as a free antiradical. Free antiradical compounds in kasumba turate plants come from secondary metabolites produced by endophytic compounds known as endophytic fungi. Kasumba turate contains phenolic compounds and carotenoids that have antioxidant activity. This study aims to obtain isolates of endophytic fungi in kasumba turate (Carthamus tinctorius (L.) plants that can act as free antiradicals by KLT-Autography method. Five isolates IFDK 01, IFDK 02, IFDK 03, IFDK 04, and IFDK 05 were obtained. After testing the antioxidant activity of endophytic fungal isolates, two isolates were obtained that gave the best antioxidant activity, namely isolates IFDK 03 and IFDK 04. The results of KLT-Autography testing by spraying DPPH isolates IFDK 03 and IFDK 04 have the potential as free antiradicals showing yellow spots on a purple background having an Rf1 value, IFDK 03 isolate 0.94 and Rf2 0.55 and Rf1 value, IFDK 04 isolate 0.76 and Rf2 0.43
Antibacterial Activity of Endophytic Fungi from Bidara Roots Against Bacteria that Cause Skin Infections Nurdayani, Sitti; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1001

Abstract

Endophytic fungi are fungi that grow and colonize plant tissues and are capable of producing bioactive compounds similar to those produced by their host. This study aims to determine the concentration of endophytic fungal isolates from Bidara roots (Ziziphus mauritiana Lam) that have antibacterial activity against bacteria that cause skin infections using the agar diffusion method. The research method begins with the rejuvenation of IFAZ-6 isolates, and microscopic and macroscopic observations are carried out. The isolates were fermented using Maltose Yeast Broth medium at 200 rpm for 21 days. The fermentate was extracted with an ethyl acetate solvent to obtain a thick extract. The tests carried out were minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibacterial activity tests carried out using the agar diffusion method. The results of the antibacterial activity test showed that the IFAZ-6 isolate extract at a concentration of 800 ppm showed antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Propionibacterium acnes. The statistical analysis results showed that the extract of isolate IFAZ-6 against Staphylococcus aureus and Staphylococcus epidermidis at concentrations of 800-1600 ppm and 1600–3200 ppm exhibited the same antibacterial activity. The bacterium Pseudomonas aeruginosa showed the same antibacterial activity at all tested concentrations, and the bacterium Propionibacterium acnes at concentrations of 1600–3200 ppm also exhibited the same antibacterial activity.
Optimization Time for Antibacterial Production of Endophytic Fungi Isolated from Bidara Root (Ziziphus mauritiana Lam.) Annisa, Khusnul; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.997

Abstract

Research on optimization time for antibacterial production of endophytic fungal isolated from bidara root (Ziziphus mauritiana Lam.) has been conducted, with the aim of determining the optimum production time for antibacterial compounds. Endophytic fungi are a group of fungi that live within plant tissues without causing harm to their host plants. In this research, the roots of bidara were isolated and purified using PDA media, isolates were followed by screening endophytic fungi isolates against test bacteria. The results of the endophytic fungal isolation from the bidara roots yielded 10 endophytic fungal isolates. The screening test against the test bacteria showed the largest inhibitory zone for IFAZ-6. Subsequently, the growth curve of these isolates was determined through fermentation using MYB media using a shaker speed of 200 rpm at a temperature of 25-28°C for 27 days. Then, mycelia and supernatant were collected on days 11, 13, 15, 17, 19, 21, 23, 25, and 27 with a 48-hour interval. The mycelia were weighed until a constant weight was obtained, while the supernatant was used to test the activity against the test bacteria using MHA media. The growth curve results indicated a stationary phase on day 21. The antibacterial activity test showed that Isolate IFAZ-6 reached optimum activity on day 27 with an inhibitory zone of 12.33 mm against Salmonella thypi.
Isolation and Identification Endophyte Fungi from Daun Nona Makan Sirih (Clerodendrum thomsoniae) as Antibacteril Against Bacteria Causing Skin Infection Using TLC-Bioautography Hadjar, Aflinda; Fitriana, Fitriana; Asmaliani, Ira
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1034

Abstract

Nona makan sirih (Clerodendrum thomsoniae) contains secondary metabolites that have the potential as antibacterials. The TLC-Bioautography method will determine the antibacterial activity and chemical components of endophytic fungal isolates of nona makan sirih. This research involved the isolation of endophytic fungi from leaves of nona makan sirih using PDAC media, followed by purification and macroscopic and microscopic tests. In addition, a screening test its effect againts pathogenic bacteria. The chosen isolates were fermented for 21 days. Then supernatant and mycelia were seperated. The supernatant was extracted by dissolving ethyl acetate using the liquid-liquid ectraction method. Then extract obtained was processed by TLC with the mobile phase of chloroform:methanol (8:1) and TLC-Bioautography was carried out on bacterial tests and chemical components. The results of isolation and purification obtained as many as 13 isolates. Screening test results with IFDNMS codes 6, 8 and 11 showed activity against the test bacteria. TLC-Bioautography result isolate 6 obtained 5 spots with an Rf value of 0.89; 0.65; 0.45; 0.23; 0.05 was active against all test bacteria. Isolate 8 obtained 5 spots with an Rf value of 0.89; 0.65; 0.05 was active against all tested bacteria, while 0.45 was active against P.acnes, S.aureus, and S.epidermidis bacteria and 0.23 against S.aureus bacteria. Isolate 11 obtained 5 spots with an Rf value of 0.89; 0.61; 0.45; 0.23; 0.05 against all test bacteria. The findings indicate that flavonoids and phenolics are chemical components with antibacterial activity.

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