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Contact Name
Yolanda Fitria Syahri
Contact Email
yolandafitriasyahri@gmail.com
Phone
+62811402721
Journal Mail Official
agrotechjournal@gmail.com
Editorial Address
Jl. Pemuda no.339 Kolaka, Indonesia
Location
Kab. kolaka,
Sulawesi tenggara
INDONESIA
Agrotech Journal
ISSN : 25485121     EISSN : 25485148     DOI : Prefix 10.31327
Core Subject : Agriculture,
The focus and scope of Agrotech Journal cover five disciplines: Plant Disease, Horticulture, Soil Science, Agronomy and plant breeding.
Articles 6 Documents
Search results for , issue "Vol 6, No 2 (2021): Agrotech Journal" : 6 Documents clear
Variations on The Concentration and Purity of Oil Palm Flowers (Elais guineensis Jac.) in Some Extraction Methods for Identification of the Flowering Gene Winda Nawfetrias; Yuda Purwana Roswanjaya
Agrotech Journal Vol 6, No 2 (2021): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v6i2.1658

Abstract

Palm oil production is related to the number of fruit and is thought to be determined since the beginning of the flowering phase as the initial stage of fruit formation. Identification of oil palm flowing genes needs to be done as the first step of knowing the mechanism of flowering in oil palm molecular. Oil male and palm females contain high polysaccharides and polyphenolic compounds so they can inhibit the process of molecular identification. Molecular studies provide accurate and fast information about the potential of oil palm genetics as a commercial commercial commodity. The purity and concentration of DNA and the temperature of annealing are the main requirements for PCR-based molecular studies. The purpose of this study obtained the extraction method of oil palm flowers which produced high DNA concentration and purity and optimized the temperature of annealing to identify the flowering gene. DNA from male flower spike and female flowers is extracted using the A (SDS) method, method B (CTAB), method C (CTAB + PVP), and method D (commercial kit). Primary BMS annealing temperature for amplification of optimized flowering genes using PCR gradients. The extraction of male flowers and female flowers using the C (CTAB + PVP) method produces the best DNA concentration and purity compared to other methods. The best annealing temperature for male flower DNA amplification and female flowers using the BMS primer is 61.1 o C. Amplification of male flowers and female flowers using Primary BMS produces 1200 PB products. The results of DNA extraction and PCR amplification using the BMS primers in female flower samples are better than male flowers
In Vitro Culture of Dragon Fruit (Hylocereus polyrhizus): Callus and Anthocyanin Production I. Indarwati
Agrotech Journal Vol 6, No 2 (2021): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v6i2.1659

Abstract

Tissue culture biotechnology has been widely used to produce secondary metabolites. Anthocyanin as the secundary metabolic content in Dragon Fruits was thought to be able to liberate free radicals and have pharmacological activities as antioxidants and anti-aging. Anthocyanin can be produced in vitrothrough callus production by tissue culture. The study aims to produce callus cultures that have the potensial to produce secondary metabolites. This research was conducted in the tissue culture laboratory. The study used a completely randomized design method with a factorial pattern. Factor 1 : Type of explants ( M1): Explants from young shoots; (M2) Explants from dragon fruit callus. Factor II: The number of explant each culture tube. There are J1,J2, 3 and J4 with (1;2;3;4 explants in each culture tube. Murashige and Skoog + 15 % sucrose were used as media culture. The results of study showed that: callus began to form at 7 weeks after planting. (1) MS Media with the addition of 15 % sucrose had a significant effect on increasing the anthocyanin content in the callus of Dragon fruit formed. (2) Callus formed from Shoot Dragon fruit contain Anthocyanin 0,74 % ; (3) The Callus formed from Shoot and callus Dragon Fruits contain secondary martabolites of Anthocyanins ranging from 0,68%–0,76%. The highest Anthocyanin content (0,76%) was produced in treatmant J3 ( 3 Explant each culture tube)
Response of Superior Maize Verieties to Different Combination of Inorganic and Organic Fertilization Rusnadi Padjung; Ifayanti Ridwan; Muh. Farid BDR; N. Nasaruddin; Muh. Fuad Anshori
Agrotech Journal Vol 6, No 2 (2021): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v6i2.1587

Abstract

Productivity of superior genotypes often did not satisfyingly achieve its genetic yield potential. Interaction between genetics and environmental factors more likely to driven the final yield of the varieties. The concept of balanced fertilization is carried out by referring to the creation of a balance of macro-nutrients in the soil so that plants can produce optimally. The research was conducted in a form of experimental study at Tarowang village, Takalar Regency during growing season of 2021 from May to September 2021. A split plot design was used with fertilization package as the main plot and superior varieties as subplots. Three fertilization packages combined inorganic and organic fertilizer were used, namely fertilization package consisted of N: P: K with ratio of 225:100:75, fertilizer package that consisted of N: P: K ratio of 200:100:50 + KNO3 25 kg + Biotani 5cc L-1, and fertilizer package that consisted of N: P: K with a ratio of 200:100:50 + KNO3 25 kg + Ecofarming 5cc L-1. Six superior varieties used were Nasa 29, JH-37, Bisi 2, Bisi 18, SINHAS 1 and NK7328. Based on the level of both factors, 18 treatment combinations were obtained and repeated three times. Responses of maize varieties to fertilization packages, consisted of different combination and dosage of NPK fertilizers, KNO3 and organic fertilizers, were varied between varieties. The treatment of fertilization package of N: P: K = 225:100:75 with Bisi 2 variety gave the highest maize productivity, namely 12.39ton ha-1
Stomata Density Analysis of Red Chili (Capsicum annuum L.) at Different Location N. Nurhaya; Asrullah Syam; Jusmiati Jafar
Agrotech Journal Vol 6, No 2 (2021): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v6i2.1660

Abstract

Red chili (Capsicum annuum L.) is a commodity of vegetable that has high economic value. Stomata are an important part of plant organs, it a gap that role in the process of photosynthesis and plant transpiration. The purpose of this study to determine differences in the stomata density of red chili (Capsicum annuum L.) leaves at different locations, namely locations exposed and shaded from sunlight. This study used a quantitative approach with the type of research used is experiment as by one factor is that sunlight intensity, used two paired treatments and respective of three repetition. The data collection method in this study used direct observation and used analytic method to analyze the stomata density of chili (Capsicum annuum L) leaves. The result of his study showed that the stomata density of red chili (Capsicum annuum L.) leaves differed according to the planting location in the form of locations exposed to sunlight and locations that were shaded. The stomata density planted in a location exposed to full sun had a higher stomata density at 409.9 cells/mm2, while the stomata density of red chili (Capsicum annuum L.) leaves planted in a shaded location had a lower stomata density at 96.9 cells/mm2 
Hydroponic Salinity Screening by Deep Flow Technique on All Paddy Growing Phases N. Nasaruddin; Muhammad Fuad Anshori; Muh. Farid BDR; Hari Iswoyo; Yunus Musa; Muh. Arifuddin; Andi Isti Sakinah
Agrotech Journal Vol 6, No 2 (2021): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v6i2.1591

Abstract

Salinity screening under hydroponic Deep Flow Technique (DFT) has not been widely studied, especially on the nature of rice tolerance to salinity stress. According to previous screening studies, this method was effective in distinguishing the nature of rice tolerance to salinity stress. However, they were tested only at generative phase. Therefore, evaluation on screening method with hydroponic DFT at all phases of paddy growth is essential. The objective of this study is to evaluate the filtering under hydroponic DFT at all paddy phase and to determine secondary character that support productivity which can be utilized as selection character in this screening process. The experiment was arranged with a complete nested group design with nested replication is the NaCl stress. There were 5 (Five) tested rice varieties and the stress environment consisted of three levels: 0, 60, and 120 mM of NaCl, all with 3 (three) replications. The nutrient culture screening was adapted to the modified Egdane method. The results showed that screening under hydroponic DFT was effective at the concentration of 60 nM of NaCl. The best selection character was yields per clump, number of productive tillers and total chlorophyll. The variety of Jeliteng, Ciherang and Inpari 34 of Salin Agritan, were classified as tolerant group. This hydroponic DFT Filtering method could be recommended as one salinity screening method for all paddy growing phases
Genetic Analysis and Distribution of F2 Population Variety of Tomato Crosses Muh. Farid BDR; M. Alfan Ikhsanul Amal; Siti Antara Maedhani Tahara; Nuriyah Magfiratul Fara Ramadhani; Ihsan Syawal Rahmat
Agrotech Journal Vol 6, No 2 (2021): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v6i2.1592

Abstract

The demand for tomatoes is increasing along with population growth. However, this demand needs to be supported by increasing tomato productivity. One way to increase this is the assembly of high-yielding tomato varieties. In the assembly of varieties, the F2 generation is a generation with high diversity. This becomes important in the process of evaluating and selecting tomato varieties. Genetic variants are the initial capital of plant breeding efforts, which are related to the assembly of varieties to increase the quantitatively and quality of the yield including fruit size, fruit quality and productivity by utilizing the heterotic phenomenon in the assembly of tomato varieties. A research was conducted to evaluate the diversity of generations of biparental crosses using an augmented design. The genotypes used were Mawar (30 plants), Karina (30 plants), F1 genotype of Mawar x Karina (25 plants), Mawar x Karina x Mawar (20 plants), Mawar x Karina x Karina (20 plants), and F2 genotype of Mawar x Karina (200 plants) which were divided into 4 blocks. The results show that the genetic diversity of the F2 population was highest in the characters of plant height, harvest age, fruit length and fruit diameter, with heritability values in the medium to high category

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