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Pusat Penelitian dan Pengabdian Masyarakat POLITEKNIK NEGERI JEMBER Jl. Mastrip Po. Box 164, Tegalgede - Sumbersari, Kab. Jember, Jawa Timur 68101. Telp (0331) 333532-34
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Jurnal Pengembangan Potensi Laboratorium
Published by Politeknik Negeri Jember
ISSN : 28284895     EISSN : 28285204     DOI : https://doi.org/10.25047/plp
Core Subject : Engineering,
Control & Systems Engineering Engineering
Jurnal Pengembangan Potensi Laboratorium merupakan media publikasi bagi pranata laboratorium Pendidikan (PLP) yang diterbitkan oleh Politeknik Negeri Jember. Jurnal ini menerima artikel ilmiah berupa hasil penelitian, survei, evaluasi, dan pengkajian ilmiah dalam bidang Pengelolaan Laboratorium yang sesuai dengan fungsi Pranata Laboratirum Pendidikan. Adapun ruang lingkup publikasi antara lain: Memodifikasi / menciptakan metode kerja (validasi). Peningkatan kemampuan instrumen/alat dalam pengujian. Pengembangan artefak/bahan acuan standar (CRM, IRM, PUP sesuai ISO 17043). Pemanfaatan bahan-bahan lokal sebagai alternatif dalam memodifikasi metode sehingga lebih efisien, efektif dan ramah lingkungan. Pengembangan metode K3 dengan pengelolaan limbah (reduce, recycle, reuse). Rancangan teknologi / membuat peralatan / bahan.
Arjuna Subject : Umum - Umum
Articles 5 Documents
 1 
Search results for , issue "Vol 2 No 2 (2023): Agustus" : 5 Documents clear
Modifikasi Media Tanam Sebagai Optimalisasi Transplanting Kultur Jaringan Anggrek Bulan (Phalaenopsis Amabilis) Ningsih, Riani; Herman Estu Eka Putra; Andre Eka Nanda
Jurnal Pengelolaan Laboratorium Vol 2 No 2 (2023): Agustus
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/plp.v2i2.3684

Abstract

Anggrek adalah salah jenis tanaman hias yang digemari oleh masyarakat pencinta bunga. Anggrek mempunyai bentuk, ukuran, jenis dan warna bunga yang sangat bervariasi dan memiliki kesegaran bunga yang relatif tahan lama, selain itu juga mempunyai nilai estetika yang tinggi. Pembiakan tanaman anggrek saat ini lebih banyak dilakukan melalui teknik kultur jaringan secara in vitro. Di Laboratorium Kultur Jaringan Politeknik Negeri Jember dalam tahap pengembangan pembiakan anggrek bulan. Selama ini PLP di Laboratorium tersebut melakukan transplanting anggrek bulan dengan media tanam yang dirasa belum sesuai dengan kebutuhan pertumbuhan anggrek bulan sehingga tidak tumbuh secara optimal. Penelitian ini bertujuan untuk memperoleh modifikasi media tanam terbaik sebagai optimalisasi transplanting kultur jaringan anggrek bulan (Phalaenopsis amabilis). Metode penelitian ini adalah pengujian dan perbandingan modifikasi media tanam transplanting anggrek dengan penambahan beberapa bahan organik sehingga dapat digunakan sebagai SOP untuk pengembangan TEFA di Laboratorium Kultur Jaringan Politeknik Negeri Jember. Parameter yang diamati dalam penelitian ini adalah Panjang tanaman, Jumlah daun, Lebar daun, Panjang Daun, Presentase Keberhasilan dan Berat Basah Planlet.
Pengembangan Metode Teknik Sterilisasi Eksplan Guna Meningkatkan Keberhasilan Kultur Jaringan Tanaman Stevia (Stevia Rebaudiana Bertoni) Cahyono, Eko Hadi; Riani Ningsih
Jurnal Pengelolaan Laboratorium Vol 2 No 2 (2023): Agustus
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/plp.v2i2.3685

Abstract

Stevia rebaudiana Bert is a shrub native to the borders of Paraguay, Brazil and Argentina. The leaves of this plant produce steviol glycosides, a secondary metabolite which has a sweetness level of 200-300 times sweeter than sugarcane sucrose. Conventional stevia propagation methods cannot meet the increasing need for stevia seeds or seedlings. Tissue culture is able to fulfill this deficiency because it has the potential to produce large amounts of uniform plantlets. Tissue Culture Technique is a vegetative propagation of plants. Tissue culture includes the cultivation of cells or cell aggregates, tissues and organs of plants in a medium containing sugars, vitamins, amino acids, organic salts, water, growth regulators and compacting agents. The composition of the growing medium is also very beneficial for the growth of fungi and bacteria. Therefore, explant sterilization is an important factor in determining the success of a tissue culture program. The problem is that until now there is no optimal explant sterilization technique for Stevia tissue culture. The aim of this research is to find out the best explant sterilization method from a combination of several disinfectants on Stevia (Stevia rebaudiana Bertoni) explants which can remove contaminants on the explants. The main parameters observed were Source of Contamination (descriptive: shape and color), Percentage of Explants Alive, Percentage of Contamination, Percentage of Browning, and Time when contamination first appeared (Early). The results showed that the sterilization method F used running water, immersion in detergent for 10 minutes, immersion in 1.5% fungicide solution and 1.5% bactericide for 10 minutes, 70% alcohol immersion for 1 minute, 0.05% HgCl2 immersion 3 minutes and immersion in 10% and 5% NaOCl respectively for 5 minutes had the lowest contamination percentage of 20% and the highest survival presentation of 80% with 80% fungal and 20% bacterial contaminants and the initial contamination occurred in the first week of observation.
Rekayasa Ketangguhan Impak Terhadap Polymer Hybrid Composite Fariasi Penambahan Serat Daun Nanas Dan Serbuk Arang Harijono, Harijono; Akhmad, Akhmad; Hartono, Hartono
Jurnal Pengelolaan Laboratorium Vol 2 No 2 (2023): Agustus
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/plp.v2i2.3686

Abstract

At the moment the demand for materials is increasing with good mechanical properties as well as economical. As an effort to meet these needs, composite materials are used. A composite is a combination of two or more inhomogeneously mixed materials consisting of a matrix and a reinforcement. Various studies conducted generally use one type of fiber so that the composite properties depend on one fiber used. The research that has been carried out has the aim of determining the effect of variations in the addition of pineapple leaf fiber and charcoal powder on the tensile strength and toughness of the impact of polymer hybrid composites. In addition, useful research provides information to readers regarding the strength and toughness of polymer hybrid composite with pineapple leaf fiber reinforcement and charcoal powder.There were three variations in the ratio of pineapple leaf fiber and charcoal powder, namely 10%:30%, 20%:20%, and 30%:10% used in the tensile test. The results showed that the tensile strength increased along with the increase in the volume fraction of pineapple leaf fiber due to good load distribution and the ability to withstand increased tensile load.The average yield of absorbent energy of polymer hybrid composites is 35.81 J, 35.526 J, and 30.93 J, respectively, while the toughness value decreases along with the increase in pineapple leaf fiber due to the bond between the fibers and the matrix that is getting weaker As for the average yield of impact prices of 0.506 J/mm2, 0.466 J/mm2, and 0.39 J/mm2, respectively Keywords: Composite, Polymer Hybrid Fiber Composite, impact, Matrix.
Kekuatan Tarik Terhadap Beberapa Sudut Serat Pada Polimer Matrik Komposit Yang Berpenguat Serat Bambu Dengan Perlakuan Di Alkali Untuk Pengembangan Bodi Dan Rangka Kendaraan Elektrik Purwanto, Hengki; Andary, Siti Rodiyah; Hartono, Hartono
Jurnal Pengelolaan Laboratorium Vol 2 No 2 (2023): Agustus
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/plp.v2i2.3688

Abstract

Composite Matrix Polymer is a material formed by two or more combinations, Composite Matrix Polymer is a material consisting of two basic materials, namely matrix and fiber. For fibers it has elastic properties, and the best tensile strength, but also cannot be used against high temperatures, while matric itself has tenacious, soft properties.And it is also binding if it is dry or has reached freezing. This research is to determine the material with natural fibers, namely bamboo fibers that have been processed to such an extent as to realize or entrepreneurial opportunities in the field of body and electric vehicle frames. For this reason, we made this research.To determine the effect of variations in the fibrous angles of composite matrix polymers with angles (0°), (-45°, 45°), and (-45°, 0°, 45°), With bamboo fiber reinforcement for the addition of bamboo fiber variations and with Alkaline treatment at the ratio of NaOH solution levels of 5%, 10%, and 15%, thus to carry out the addition of alkaline treatmentThis study uses experimental methods, and can determine the influence of angular variations, composite matrix polymer fibers with fiber angles (0°), (-45°, 45°), and (-45°, 0°, 45°) reinforced bamboo fibers, with the influence of variations in alkaline dosing of bamboo fibers and the ratio of NaOH solution levels of 5%, 10%, and 15% on the tensile strength of the material.The result of the tests that we have done, is the use of a good 5% alkaline content, a 5% alkaline solution content gets the best fiber speciment form than a 10% and 15% alkaline solution. And the lowest result is an alkaline content of NaOH 15%. While the best result of angle variation is the angle variation (-45°, 0°, 45°) and the lowest is the angle variation (0°). Which will then be used as a method for learning or practicum modules at the Jember State Polytechnic.
Modifikasi Metode Freezing dengan Krioprotektan DMSO Terhadap Kualitas Penyimpanan Mikroba Saccharomyces cereviseae Di Laboratorium Biosain Cholifah Ida, Novita; Sariono, Hadi; Widyawatiningrum, Endang
Jurnal Pengelolaan Laboratorium Vol 2 No 2 (2023): Agustus
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/plp.v2i2.3727

Abstract

Proses perbanyakan dan penyimpanan kultur mikroba menjadi hal yang harus diperhatikan karena cara penyimpanan sangat terkait dengan perubahan sifat mikroba yang disimpan. Di Laboratorium, kultur murni dipindahkan dengan metode subkultur. Beberapa dampak subkultur yang berulang akan memakan waktu, beresiko kontaminasi dan mutasi genetik. Penelitian ini bertujuan untuk mengembangkan penyimpanan mikroba Saccharomyces cereviseae jangka panjang yang aplikatif di Laboratorium Biosain serta menentukan jenis Cryoprotectan Agent yang dapat mempertahankan viabilitas sel mikroba Saccharomyces cereviseae dengan metode Freezing. Dalam penelitian sebelumnya peneliti menggunakan metode preservasi cryogenic freezing dengan menggunakan gliserol. Gliserol atau sukrosa dapat berperan sebagai krioprotektan, namun keduanya juga dapat mengakibatkan kematian sel akibat toksisitas dari krioprotektan tersebut. Penggunaan agensia krioprotektan DMSO 5 % dan gliserol 10 % dapat direkomendasikan untuk di gunakan sebagai referensi simpan beku (freezing) sel Saccharomyces cereviseae di Laboratorium Biosain dan merupakan metode penyimpanan jangka panjang yang paling efektif untuk dilakukan. Hasil penelitian menunjukkan agent krioprotektan yang menghasilkan persentase penurunan viabilitas paling stabil dengan metode freezing adalah krioprotektan DMSO 5 % dengan persentase penurunan viabilitas sebesar 3,28 % untuk penyimpanan selama 1 bulan, persentase penurunan viabilitas sebesar 4,10 % untuk penyimpanan selama 2 bulan dan persentase penurunan viabilitas sebesar 16,39 % untuk penyimpanan selama 3 bulan. Kemudian diikuti agent krioprotektan gliserol 10 % dengan persentase penurunan viabilitas sebesar 5,74 % untuk penyimpanan selama 1 bulan, persentase penurunan viabilitas sebesar 7,38 % untuk penyimpanan selama 2 bulan dan persentase penurunan viabilitas sebesar 18,20 % untuk penyimpanan selama 3 bulan.

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