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Tika Hairani
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Kantor Sekretariat MABBI, Bioteknologi-Universitas Esa Unggul, Jl. Raya Arjuna Utara No. 9, Duri Kepa, Kebon Jeruk, Jakarta Barat 11510
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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia
ISSN : 24422606     EISSN : 2548611X     DOI : https://doi.org/10.55981/jbbi
Core Subject : Science,
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general. JBBI terbit dua kali setahun dan menyediakan media penerbitan ilmiah bagi para peneliti, insinyur, praktisi, akademisi, dan pemerhati di bidang yang terkait dengan bioteknologi dan biosains. Jurnal ini menerima naskah asli hasil penelitian, naskah ulasan, studi kasus, dan komunikasi singkat. Naskah yang diterbitkan adalah peer-review oleh tidak kurang dari dua orang penelaah, dan mencakup berbagai cabang bioteknologi yang terkait dengan bidang pertanian, industri, kesehatan, lingkungan, bioinformatika, serta ilmu kehidupan pada umumnya.
Articles 21 Documents
Search results for , issue "Vol. 12 No. 1 (2025)" : 21 Documents clear
KINEMATICS OF SPERMATOZOA IN FRESH AND FROZEN SEMEN OF PESISIR CATTLE Ramadhan, Rio; Ananda; Hendri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11410

Abstract

This study aimed to evaluate the quality and kinematics of spermatozoa in fresh and frozen semen of coastal cattle. Semen of coastal cattle was collected using an artificial vagina from 2 males, fresh semen was tested and frozen using tris egg yolk diluent. Fresh and frozen semen of coastal cattle were evaluated using a Computer Assisted Sperm Analyzer (CASA). This study used a t-test analysis by comparing the quality and kinematics of spermatozoa of fresh semen and frozen semen of coastal cattle. The parameters observed were macroscopic, microscopic and CASA quality observations. The results showed the kinematics of fresh and frozen semen motility (83.83%; 71.68%) and the kinematics of progressive motility of fresh and frozen semen (80.81%; 59.56%). Based on the results of the t-test analysis, it was found that t count > t table, the kinematics of fresh semen spermatozoa had a significant effect on the frozen semen of Pesisir cattle on the motility of fresh semen and frozen semen, on the progressive motility of fresh semen and frozen semen. The characteristic values ​​of kinematics in fresh semen and frozen semen of Pesisir cattle were respectively velocity curvilinear (VCL) 178.94 μm/s and 108.52 μm/s; velocity straight line (VSL) 96.61 μm/s and 49.34 μm/s; velocity average path (VAP) 114.99 μm/s and 64.04 μm/s; linearity (LIN) 0.53% and 0.44%; straightness (STR) 0.83% and 0.75%; wobble (WOB) 0.64% and 0.58%; lateral head displacement (ALH) amplitude 5.83 μm and 5.15 μm; beat cross frequency (BCF) 35.28 Hz and 24.65 Hz. The kinematics of fresh and frozen semen spermatozoa significantly affect the value of motility characteristics. The conclusion of the results of this study is that there is a decrease in the quality of fresh semen kinematics and frozen semen kinematics spermatozoa on the value of motility characteristics, namely on the speed of spermatozoa at the velocity curvilinear (VCL), velocity straight line (VSL), and velocity average path (VAP), linearity (LIN), straightness (STR), and wobble (WOB) and on the distance value of spermatozoa amplitude lateral head displacement (ALH) and beat cross frequency (BCF).

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