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Chem Info
Published by Universitas Diponegoro
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Core Subject : Science, Engineering,
Chemical Engineering, Chemistry & Bioengineering Chemistry
CHEMINFO JOURNAL adalah jurnal saintifik yang diterbitkan secara periodik 3 bulanan oleh Jurusan Kimia, Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Jurusan Kimia.
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Search results for , issue "Vol 1, No 1 (2013)" : 50 Documents clear
PEMURNIAN SELULASE DARI ISOLAT KB KOMPOS TERMOFILIK DESA BAYAT KLATEN MENGGUNAKAN FRAKSINASI AMONIUM SULFAT Sinatari, Hanum Mustika
Chem Info Journal Vol 1, No 1 (2013)
Publisher : Chem Info Journal

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Abstract

Purification of cellulase enzymes derived from KB thermophilic composting isolates of Bayat Klaten using fractionation ammonium sulphate needs to be done so that cellulase can cuts cellulose chains more effectively. This study aims to gain cellulases from KB thermophilic composting isolates of Bayat Klaten is more pure than the crude cellulase extract. Purification of cellulases from KB isolates using fractionation ammonium sulphate with saturation 0-20% (F1), 20-40% (F2), 40-60% (F3), 60-80% (F4), and 80-100% (F5), followed by cellulase dialysis using cellophane membranes. Cellulases fractions activity was measured using Dinitrosalicylic acid (DNS) method and Lowry method. This study obtained cellulase with specific activity in the crude extract cellulase of 0.212 units/mg protein and the highest enzyme specific activity in fraction 2 (20-40% ammonium sulphate) of 0.749 U/mg protein. The specific activity of cellulase in fraction 2 increased 3,5 times from the specific activity of the crude cellulase extract.  
Pengaruh Ion Logam Co2+ dan Cu2+ Pada Proses Fotodegradasi Direct Blue 3R Menggunakan Fotokatalis Komposit ZnO-Karbon Aktif Wicaksono, Aji Putro
Chem Info Journal Vol 1, No 1 (2013)
Publisher : Chem Info Journal

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Abstract

Been conducted synthesis and characterization of ZnO-activated carbon composites, and examines the impact of the presence of metal ions Co2+ and Cu2+ in the process of photodegradation Direct Blue 3R and photoreduction metal ions. Synthesis of ZnO with the XRD results obtained ZnO (wurzite) D = 27.97 nm; Eg = 3.8 eV. Composite ZnO-activated carbon produced in tablet form with d = 0.8 cm; t = 0.45 cm gray. SEM and BET characterization of the resulting composite has a hollow morphology with a surface area ZnO-carbon composite activated by 27.209 m2/g, pore volume of 0.089 cc/g; finger pores of 3.25 nm and has a high effectiveness in the photodegradation of dye Direct Blue 3R of 95.36%. The optimum pH for photodegradation process is at pH 4 with the effectiveness of 91.92%. The addition of metal ions Co2+ and Cu2+ can increase the effectiveness of photodegradation Direct Blue 3R and can simultaneously reduce metal ions Co2+ and Cu2+ into Co(s) and Cu (s) with the optimum concentration of each metal is 12 ppm. The optimum time for photodegradation process is 2 hours. Photodegradation effectiveness of 94.875% and 76.31% on the addition photoreduction Co2+, whereas the addition of Cu2+ photodegradation effectiveness of 96.208% and 89.33% photoreduction.
FERMENTASI RUMPUT LAUT Eucheuma cottonii OLEH Lactobacillus plantarum Wandansari, Baiq Daraquthni
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

Eucheuma cottonii has been known contain nutrients such as proteins, lipids, carbohydrates, vitamin C, α-tocopherol, and minerals. Nutrient content of seaweed can be utilized as a medium for the growth of lactic acid bacteria (LAB). Fermented seaweed by LAB can be used as probiotic drinks for patients with lactose intolerance. Fermentation by LAB produces primary metabolites like lactic acid, CO2, acetaldehyde and hydrogen peroxide. This study aims to obtain characteristic data of fermented E. cottonii of West Lombok, NTB by Lactobacillus plantarum (texture, color, smell, taste and pH). Fermentation E. cottonii by L. plantarum performed by addition of 5% glucose and 5% inoculum, and then incubated at 37 ° C for 72 hours. The results showed that the fermentation of E. cottonii by L. plantarum produce fermented seaweed with characteristics: yellow-green jelly, yogurt sour odor, has a sour taste and slightly bitter with a pH of 3.
PEMANFAATAN LIMBAH KULIT UDANG WINDU (Penaeus monodon) SEBAGAI EDIBLE COATING DAN PENGARUHNYA TERHADAP KADAR ION LOGAM Pb(II) PADA BUAH STROBERI (Fragaria x ananassa) Marzuki, Qosim
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

A study on the utilization of leather wastes tiger shrimp (Penaeus monodon) as an edible coating and effect of Pb (II) in strawberries (Fragaria x ananassa). This research aims to synthesize chitosan from chitin of the shrimp shell waste, to determine the optimum concentration of chitosan as edible coating and determine the effect of chitosan to adsorption Pb (II) on the surface of strawberries. Experiment were carried out in several stages of research starting with the isolation of chitin by demineralitation and deproteination and than transforming it into the chitosan by deacetylation. The result is then analyced by of FTIR. Further manufacture of edible coating, the coating of fruit, and analysis of fruit quality after coating, including testing of acidity (pH) strawberries, Vitamin C, and test Pb metal adsorption on the surface of the fruit, and organoleptic test was done visual observation of physical appearance of strawberries. The results obtained chitosan can be synthesized from shrimp shell waste with 70.03% degree of deacetylation. Chitosan 2% as the coating was able to maintain the qulity of strawberries fruit longer than without coating until the fifth day. Chitosan as coating agent was also able to reduce the presence of metal ions Pb2+ on the strawberries surface
AKTIVITAS Fusarium oxysporum DALAM MENGHIDROLISIS ECENG GONDOK (Eichhornia crassipes) DENGAN VARIASI WAKTU FERMENTASI Tristianti, Siska Yulyana
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

Fusarium oxysporum activity to hydrolyze water hyacinth (Eichhornia crassipes) with variations of fermentation time taken to get the Fusarium oxysporum that have adapted to the fermentation medium of water hyacinth (Eichhornia crassipes) and obtain activity data of Fusarium oxysporum in hydrolyzing water hyacinth (Eichhornia crassipes) on variations of fermentation time. Activities Fusarium oxysporum based on the ability to hydrolyze water hyacinth mass accretion based cell (dry weight) produced. This research found that Fusarium oxysporum capable of growing on water hyacinth fermentation media and the activity of Fusarium oxysporum to hydrolyze water hyacinth occurred at optimum fermentation time at hours-168 which is the exponential phase of Fusarium oxysporum.
Pembuatan Nanozeolit dari Zeolit Alam Secara Top Down Menggunakan High Energy Milling dan Aplikasinya untuk Penyerapan Ion Fe 3+ Agusetiani, Lilis
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

Has done research on facbrication nanozeolites from natural zeolite in top down use high energy milling and its application to adsorption Fe3+ Ions. The objective of this research is to fabrication nanozeolites from natural zeolite  in top down using high-energy milling and determine  levels of Fe3+ ions that can be adsorbed by the zeolite usual and zeolite nano. The method  used  in  this  research  for  the  fabrication  nanozeolites  from  natural  zeolites  in  top down using high energy milling. Natural zeolites are sieved using a 225 mesh sieve and then milling  with  high  energy  milling  with  rotational  speed  of  1000  rpm  for  6  hours.  Natural zeolites product milling were activated by HF 1% and NH4Cl 2M. The results of research on natural zeolite pass 225 mesh  sieve, natural zeolite after milling, and natural zeolite milling activation characterized using SEM and   BET  that  indicates  the size of surface morphology with specific surface of 125-250 nm, 75-100 nm, 50-75 nm and 33.997 cm3/g, 29.399  cm3/g, and  86.322  cm3/g,  respectively. Concentration  Fe3+ ions was  analyzed  using AAS with  an optimum  time  of  each  45 minutes,  15 minutes,  and  150 minutes with  the  concentration  of Fe3+ ions are adsorbed were164.98 ppm, 168.65 ppm, and 146.52 ppm
PENGARUH JENIS PELARUT PADA EKSTRAKSI KURKUMINOID DARI RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb) Nurvita, Dyah Lasna
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

This study concerned in the effect of solvents on the extraction of curcuminoids from temulawak rhizome (Curcuma xanthorrhiza Roxb). Curcuminoids is one of the bioactive chemical constituents in rhizome of temulawak, known to have many benefits in the field of health and food. Bioactive ingredients may be extracted using ethanol, acetone, and ethyl acetate as solvent. Research that tries to investigate different solvents to obtain appropriate solvent in extracting has yet to be reported. This research was done by extraction using ethanol, acetone, and ethyl acetate, and defatization using n-hexane. The curcuminoids content was determined by UV-visible spectrophotometer at maximum wavelength of 427 nm. The results showed that acetone is the most appropriate solvent for the extraction of curcuminoids. It demonstrated the highest levels of total curcuminoids extracted with or without defatization
PENENTUAN TOTAL SENYAWA FENOLAT DAN AKTIVITAS ANTIOKSIDAN PADA ASAP CAIR DARI AMPAS TEBU DAN KULIT TEBU (Sacharum Officinarum) SERTA IDENTIFIKASI KOMPONEN PENYUSUNNYA ., Sulistiyowati
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

Abstract: Sugarcane (Saccharum officinarum) is a plant that is grown for raw sugar, not only for raw sugar, but it could be material for liquid smoke. A study concerning the determination of total phenolic compounds and antioxidant activity of liquid smoke sugarcane pulp and cane skin as well as the identification of its constituent components. This research was conducted to determine levels of total phenolic compounds, antioxidant activity and identification of chemical components in the liquid smoke from sugarcane pulp and sugarcane skin. Liquid smoke is made by means of pyrolysis. Pyrolysis is the thermal degradation of organic materials decompose at high temperatures in the absence of oxygen. One of the processes that result in degradation of the lignin phenolic compounds. The content of total phenolics was determined using the Folin Ciocalteu reagent were expressed as the number of mg gallic acid equivalents per g of extract. antioxidant activity assay by the method of DPPH (1,1-diphenyl-2-picrylhydrazyl) and the identification of its constituent components using GCMS. The results showed levels of total phenolic compounds for liquid smoke sugarcane pulp and cane skin respectively 68.083 mg GE / g and 171.417 mg GE / g liquid smoke, as well as price IC50 of each sample at 283.586 ppm and 216.58 ppm. The identification of its constituent components using GCMS both samples have the same dominant compounds are phenol, furan, acetic acid and syringol.
IDENTIFIKASI ASAM FENOLAT DARI EKSTRAK ETANOL DAUN BINAHONG (Anredera cordifolia (Ten.) Stennis) DAN UJI AKTIVITAS ANTIOKSIDAN Ekaviantiwi, Tyas Ayu
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

Binahong (Anredera cordifolia (Ten.) Stennis) is one of the flower planting that come from Basellaceae family and has been know it has biological activities because there is bioactive compound. Phenolic acids are bioactive compound and widely used as antioxidants. In this study, identification and antioxidant activity test in ethanolic extract of binahong leaves were done. Phenolic acids were isolated without hydrolysis (TH fraction), acid hydrolysis (fraction HA), and alkaline hydrolysis (HB fraction). Based on the identification using TLC with co-chromatography, UV-Vis and FTIR spectrophotometer, detected that ethanolic extract of binahong leaves maybe contain p-coumaric acid. The quantitative analysis using TLC scanner known that the levels of p-coumaric acid of TH fraction, HA fraction, HB fraction are 8.11205%; 3.77526%; and 23.57104% respectively. The antioxidant activity (IC50) of ethanolic extract and isolate B are 866.89831 mg/L and 1263.3333 mg/L respectively. This indicates that B isolates haven’t the potency to be developed as an antioxidant compound cracked.
KONSENTRASI HAMBAT MINIMUM (KHM) KADAR SAMPEL ALANG-ALANG (Imperata cylindrica) DALAM ETANOL MELALUI METODE DIFUSI CAKRAM Mulyadi, Moh
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

Bacteria are microorganisms arround us. Recent study was sought an alternative source that serves as an antibacterial because some pathogenic bacteria to be resintant with antibacterial. Materials are reported to have antibacterial activity among the reeds. Reed efficacious drug for acute kidney inflammation, antibacterial, vomiting blood, gonorrhea, and nosebleeds. This study aimed to obtain the Minimum Inhibitory Concentration (MIC) of the sample degree as an antibacterial reeds and obtain the data most potent antibacterial activity of the sample degree against the reeds to Escherechia coli, Pseudomonas aeroginosa, Staphylococcus aureus and Bacillus subtilis. One of the methods method used is the preformance test antibacterial paper disk diffusion method. This method is done by placing a paper disc that had been soaked in the test solution on solid media has been inoculated with the bacteria test. Once inoculated, the bacterial growth was observed to see the clear zone around the disc. Zone inhibitation that is formed around the discs at the lowest concentration of antibacterial is the MIC. MIC from the sample degree of the reeds against Escherechia coli bacteria, Pseudomonas aeroginosa, Staphylococcus aureus and Bacillus subtilis in a row of leaf sample degree in ethanol by 7%, 7%, 8% and 9%, for the sample degree flowers in ethanol of interest at 7%, 7%, 9% and 7%, and for the sample degree roots in ethanol of 7%, 8%, 10% and 8%. The third sample degree of reeds showed a good potential to inhibite bacteria Escherichia coli. The sample degree of leaves and flowers are more potential than roots of reeds to inhibite Pseudomonas aeroginosa. On the other hand, the most potential for

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