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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 7 Documents
 1 
Search results for , issue "Vol. 12 No. 1 (2018): March 2018" : 7 Documents clear
THE EFFECT OF CARBON AND NITROGEN SUPPLEMENTATION ON BACTERIOCIN PRODUCTION OF LACTIC ACID BACTERIA FROM PICKLED YELLOW BAMBOO SHOOTS (Dendrocalamus asper) LAKSMI HARTAJANIE; LINDAYANI LINDAYANI; LORENTIA SANTOSO
Microbiology Indonesia Vol. 12 No. 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3837.952 KB) | DOI: 10.5454/mi.12.1.2

Abstract

Six selected lactic acid bacteria (LAB) isolates from pickled Yellow Betung bamboo shoots were grown in de Mann Rogosa Sharpe-Broth (MRSB) media with different supplementation combination. The cell-free supernatant were evaluated for their ability to produce bacteriocin  by adjusting its pH to 6.0 in order to remove organic acid effects. The bacteriocin activity was  assayed by agar-well diffusion method. The inhibitory activity calculated in Activity Unit (AU in mm2 mL-1) of bacteriocins. The aims of this paper is to explore the effect of different medium compositions on bacteriocin production and its inhibitory activity against pathogenic bacteria (Listeria monocytogenes FNCC 0156, Staphylococcus aureus FNCC 0047, and Escherichia coli FNCC 0091).Supplementations of carbon and nitrogen sources induced production of bacteriocins. LAB isolates grown in media without supplementation could not produce bacteriocins.  Growth of isolate D44 in the presence of 2% of glucose and 2% of yeast extract yielded the largest bacteriocin inhibitory activity levels of 3179 AU mL-1 against Listeria monocytogenes FNCC 0156, 4663 AU mL-1 against Staphylococcus aureus FNCC 0047, and 3109 AU mL-1 against Escherichia coli FNCC 0091.
Detection of Antibody to Burkholderia pseudomallei in Captive and Wild Macaquesin West Java and Bali, Indonesia VINCENTIUS ARCA TESTAMENTI; DIAH ISKANDRIATI; ARIS TRI WAHYUDI; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 12 No. 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.83 KB) | DOI: 10.5454/mi.12.1.4

Abstract

Melioidosis is an emerging zoonotic disease caused by the saprophytic Burkholderia pseudomallei, which infects human and a wide range of animal species. Melioidosis may lead to septicemia and pneumonia in human patients, which can be fatal if the patient is not treated accordingly. The disease is spread in tropical areas and is highly endemic to Southeast Asia and Northern Australia. However, melioidosis is poorly reported in Indonesia, especially in the veterinary field. This research provides serological evidence of antibodies to B. pseudomallei in both captive and wild nonhuman primates. Plasma samples were taken from a total of 390 monkeys in captivities and wild habitats in West Java and Bali, Indonesia. Enzyme-linked immunosorbent assay (ELISA) showed that the seroprevalence was 42.21% for Macaca fascicularis and 43.59% for Macaca nemestrina. Furthermore, the seroprevalence was 53.41% for captive macaques and 17.83% for wild macaques. The findings showed that exposure to B. pseudomallei happened in both captive and wild macaques. Based on this serosurveillance results, further studies such as comprehensive culture and clinical study are required to discover the clinical burden of the disease in nonhuman primates.
Bacterial leaching of an Indonesian complex copper sulfide ore using an iron-oxidizing indigenous bacterium
Microbiology Indonesia Vol. 12 No. 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2002.316 KB) | DOI: 10.5454/mi.12.1.1

Abstract

The bioleaching of an Indonesian complex copper sulfide ore was studied in shake flasks over a period of 14 days using an iron-oxidizing indigenous bacterium at room temperature (28 oC) and various pulp densities (5% and 20%). The bioleaching suspensions were periodically analyzed for Cu and Fe concentrations as well as Eh, pH and DO values. Cu bioleaching efficiencies at 5% pulp density were higher than those at 20% pulp density, which correlated with Fe concentration in solution. Over a period of 14 days, the pH of bioleaching suspension was in the range of 5 ~ 9, indicating that Cu bioleaching was greatly influenced not only by proton H+ dan ferric ion but also by extracellular polymeric substances (EPS) generated by the bacterium. The current study may improve our better understanding on the bacterial action for bioleaching of complex copper sulfide ores that remains debated so far as refractory ores.
Physiological Profiling and Microorganism Community Analysis of Cirebon Shrimp Paste Fermentation “Terasi” using BIOLOGTM EcoPlate
Microbiology Indonesia Vol. 12 No. 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (551.612 KB) | DOI: 10.5454/mi.12.1.3

Abstract

Terasi or shrimp paste is an Indonesian traditional seasoning made from fermented small shrimp or krill. Different indigenous microorganism community exhibit different physiological function due to lack standard in its materials and processing. This study aimed to determine physiological profiles and microorganism community in Cirebon shrimp paste fermentation. BIOLOGTM EcoPlate was used to obtain microbial physiological function of the krill and 2-months old shrimp paste. Microorganisms were later isolated from EcoPlate substrate to determine its community structure. Average Well Color Development (AWCD) from krill was thirty times higher than shrimp paste. Interestingly, this study revealed a shift of carbon source utilization at day-28 of fermentation from amino acid and polymer to phenolic compound. In addition, AWCD index increased in accordance with increased of microorganism community complexity at day-28. Within 56 days of fermentation there was a slightly increase in water, fat, and carbohydrate content. In contrast, there was decrease in protein, ash content, and acidity level from neutral to acid, with salinity level resulted in between 16.26% to 21.42%. We conclude that there is a change of microorganism community within shrimp paste fermentation corresponding to metabolism activity which affects the product quality. 
Probiotic Potential of Lactic Acid Bacteria from Yellow Bamboo Shoot Fermentation using 2.5% and 5% Brine at Room Temperature LINDAYANI LINDAYANI; LAKSMI HARTAJANIE; MONIKA PALUPI MURNIATI
Microbiology Indonesia Vol. 12 No. 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1089.648 KB) | DOI: 10.5454/mi.12.1.5

Abstract

Yellow bamboo shoot is a popular food material especially in Semarang because it is used as filling for lumpia (authentic food of Semarang). Beside used as filling, yellow bamboo shoot commonly known by Indonesian people. Considering bamboo shoot often processed into fermented traditional food, yellow bamboo shoot also potential to be examined as source of fermented food producing lactic acid bacteria. Lactic acid bacteria still become the most beneficial microorganisms associated with gastrointestinal system and moreover for obesity diet. The aim of this research is to study the probiotic potential of lactic acid bacteria produced from yellow bamboo shoot fermentation in 2.5% and 5% brine under room temperature (30 °C). From isolation, 22 single colonies obtained from 2.5% brine and 27 isolates obtained from 5% brine. The morphology and physiology analysis resulted in Lactobacillus and Streptococcus genus. All isolates were tested subsequently for probiotic potential. Based on the result, more than 50% identified isolates have probiotic potential.
Cover and editor information
Microbiology Indonesia Vol. 12 No. 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.517 KB) | DOI: 10.5454/mi.12.1.%p

Abstract

ITA registration and back cover Is Helianti
Microbiology Indonesia Vol. 12 No. 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1287.638 KB) | DOI: 10.5454/mi.12.1.%p

Abstract

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