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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 6 Documents
 1 
Search results for , issue "Vol. 13 No. 3 (2019): September 2019" : 6 Documents clear
The Use of Agrobacterium sp.I3 and Compost as Chelator Combined by NPK 3 Fertilizer and Mendong Plant (Fimbristylis sp.) in Bioremediation of Paddy Soil Contaminated by Lead (Pb) RETNO ROSARIASTUTI; ABDI LEONARDO SARAGIH; SUDADI SUDADI; SUPRIYADI SUPRIYADI; WIWIN WIDIASTUTI
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1888.536 KB) | DOI: 10.5454/mi.13.3.1

Abstract

Industrial waste supplies contains heavy metals such as Pb which will cause pollution in paddy fields. Remediation of paddy soil contaminated by Pb heavy metal must be done by simple, environmental friendly, cheap and sustainable technology, that is bioremediation. The purpose of this study was to study the effectiveness of bioremediation using Agrobacterium sp. I3 and compost as chelator combined by Mendong plant and NPK fertilizer, and learn the ability of Mendong in uptaking metal soil Pb. This was field experimental research, had a factorial patern, using Completly Randomized Block Design as the base design, with three factors: (1) NPK fertilizers (P0: no NPK fertilizers, P1: with NPK fertilizers), (2) Chelator (K0: no chelator; K1: with chelator Agrobacterium sp. I3 ; K2: with chelator compost); and (3) Plant (T0: without plant; T1: with Mendong plant). The results showed that Agrobacterium sp. I3 and compost were increasing Pb uptake in shoot, but decreasing Pb uptake in root. Mendong plant has highly ability in uptaking soil Pb, so decreased soil Pb, and effective as the phytoremediator. NPK fertilizer increased plant growth so increased Pb uptaken by plant. The highest Pb uptake was in treatment combination of NPK fertilizer + Mendong plant: 80.916 µg, followed by NPK fertilizer + Agrobacterium sp. I3 + Mendong Plant: 76.363 µg. The highest decreased of soil Pb (42.41%) was found in treatment combination of compost + Mendong Plant. Key words: Agrobacterium sp.I3, compost, Fimbristylis sp, Pb, phytoremediation
The Utilization of Arbuscular Mycorrhizal Fungi for Planting Agarwood (Aquilaria spp) Seedling in Open Land ABDURRANI MUIN
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (680.998 KB) | DOI: 10.5454/mi.13.3.2

Abstract

Agarwood is a type of semi-tolerant plant, so that for planting the seedlings should be grown under the shade. For planting in open land, it requires treatment in which one of them is using seedlings inoculated with arbuscular mycorrhizal fungi. The aim of the research is to obtain information on agarwood growth that has been inoculated with fungi mycorrhizal arbuscular when planted in the open land and ability to grow between agarwood seedlings inoculated mycorrhizal that was planted in the shade and in the open area. Split plot randomized block design was applied with treatments: the first plot consisting of plant had been inoculated with mycorrhizae and without mycorrhizal inoculation, and the sub plot was the types of shading that consists of open land, paranet 60 % intensity and natural vegetation. To reduce variabilty of site topographical differences were separated as bloks. Variables measured were: plant height (cm), stem diameter (mm), number of leaves, and survival percentage of plant. The results show that the height and diameter growth of seedlings innoculated with mycorrhizae were higher than non innoculated. The seedlings innoculated with mycorrhizal fungi were planted in the paranet shading grew better and significantly different compared to the vegetation shading. Seedlings innoculated mycorrhizal that were planted in open land grew better and significantly different compared to vegetation shading. This study results indicate that planting agarwood in the open land can be done using seedlings inoculated arbuscular mycorrhizal fungi. Key words: agarwood plant, arbuscular mycorrhizal fungi, shading and open land
Antibacterial Potential of Radish Extract (Raphanus sativus L.) Against Fish Spoilage Bacteria EVELINE EVELINE; CHIKITA WINI TANUMIHARDJA
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.61 KB) | DOI: 10.5454/mi.13.3.3

Abstract

Radish (Raphanus sativus L.) root is commonly used  as flavor enhancing additive or side dish. Previous research revealed the presence of active compound in which could inhibit bacterial growth. Thus, a research concerning natural antibacterial for fish products that are categorized as high-risk food being contaminated by spoilage bacteria (Pseudomonas aeruginosa, Bacillus cereus, dan Staphylococcus aureus) was done. Radish root extraction was made by using ethyl acetate (semi polar) for 3 days. Well diffusion was performed using 4 extract concentration (10, 20, 30, dan 40% (w/v)) againts  three fish spoilage bacteria. Based on our results, 30% concentration was the best concentration which inhibit more than 10 mm in inhibition zone with MIC  and MBC. The scores were of 0.06% and 0.24% (P. aeruginosa), 0.13% and 0.50% (S. aureus), and 0.12% and 0.48% (B. cereus). Moreover, based on stability test against heating temperature showed that this extract concentrationwas more stable in 80°C with duration times for 5 minutes and pH 3 which resulting the lowest inhibition zone reduction compares to control extract. Radish extract was categorized as low toxic compound (LC50 = 839.52 ppm) functioning in antibacterial compound containing major antibacterial compound (bis(2-ethylhexyl) phthalate, 1,2-benzenedicarboxylic acid, 9,12,15-octadecatrienoic acid), fatty acid (n-hexadecanoic acid, butanedioic acid), carboxylic acid (isobutyric acid, malic acid, oleic acid), and minor antibacterial compound (n-Hydroxymethylacetamide, 2,4-bis(1,1-dimethylethyl), 2,4-pentanedione,2-Cyclohexen-1-one, hydrazine, cyclohexene oxide, gamma-sitosterol).
Levels of CXCL10 Chemokine in Dengue Infected Hepatocyte Huh 7 it-1 Cell Line Co-cultured with Peripheral Blood Mononuclear Cells BETI ERNAWATI DEWI; EVA DAMAYANTI; TJAHJANI MIRAWATI SUDIRO; AGUS SYAHRURACHMAN
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (606.52 KB) | DOI: 10.5454/mi.13.3.4

Abstract

Dengue is a mosquito borne virus that spreads rapidly in the world. At present, it is estimated that more than 3.9 billion people are at risk of being infected with dengue virus (DENV) and there are 96 million clinical cases that have been reported annually in 128 countries worldwide. In DENV infected patients often associated with liver dysfunction which hepatocyte and kuppfer cells as the main target of viral infections. DENV infection induced the expression of several chemokines, which might play an important role during the inflammatory response and pathogenesis of a disease. CXCL10 is known as a chemokine that activates lymphocytes for innate and adaptive immunity, induces tissue damage, and modulates tumor formation. Therefore, we conducted an in vitro study using Huh 7it-1 cells co-cultured with peripheral blood mononuclear cells (PBMCs) to investigate CXCL10 chemokine induction during DENV infection. Huh 7it-1 cells were grown on 96 micro well plate until a monolayer was formed. The cells were infected with DENV-2 at an MOI of 0.5 FFU/cell and 1 FFU/cell in the presence of PBMCs. Heat inactivated DENV-2 and Huh7 cell medium were used as control. After 2 hours of infection, cells were co-cultured with PBMCs and incubated at 37 ºC with 5% CO 2 for 48 h. Cell supernatant was collected and CXCL10 chemokine levels were measured using CXCL10 Quantikine ELISA Kit. Statistical analysis was performed by SPSS 23. In the presence of PBMCs, CXCL10 levels from DENV infected Huh 7it-1 at an MOI of 0,5 FFU/cell and MOI of 1 FFU/cell were 552,653 ± 22,779 pg  mL-1 and 576,787 ± 16,901 pg  mL-1 . Those levels were higher when compared with supernatan from heat inactivated DENV-2 and control cells. Without PBMCs, all of treatments showed lower level of CXCL10. DENV-2 infection in Huh 7it-1 cells co-cultured with PBMCs was able to induce CXCL10 secretion. Furthermore, heat inactivated DENV-2 also still capable to inducen the secretion of CXCL10 chemokine in Huh 7it-1 cells.
The Antifungal Activity of Artesunate toward Candida albicans: Two Opposite Activities MOHAMMAD RIZKI F ADHIL PRATAMA; DARYONO HADI TJAHJONO; TUTUS GUSDINAR
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1503.088 KB) | DOI: 10.5454/mi.13.3.5

Abstract

The artemisinin and its derivatives antifungal activity continue to be an interesting research object, with the potential shown to be developed as an antifungal compound. Artesunate, one of the artemisinin derivatives known to have antifungal activity against various pathogenic fungi, including Candida albicans. This study aims to determine the effect of artesunate on antifungal activity toward C. albicans in vitro at concentrations below 1 mg -1m L . The method used is yeast-plate count, with a parameter of observation were the number of C. albicans colonies viable after exposure with artesunate for five days. The concentration of artesunate used was divided into -1 -2 -3 -4 -1si x groups, which were 10; 1; 10 ; 10 ; 10 ; and 10 mg mL . Compared to control, a significant decrease in -1c olony counts was only shown at the highest concentration of 10 mg mL . Interestingly, at the lowest -4 -1c oncentration of 10 mgmL , it showed an increase in a number of colonies almost twice of the blank. These results suggest that while at higher concentration of artesunate may inhibit the growth of C. albicans, a lower concentration of artesunate may stimulate their growth.
ITA registration form and back cover Is Helianti
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1664.726 KB)

Abstract

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