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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 3 Documents
 1 
Search results for , issue "Vol. 17 No. 2 (2023): June" : 3 Documents clear
The Effect of Pili Protein of Klebsiella pneumoniae 65,5 kDa on Enhanced IFN- Gamma Levels in Mice Liver Dini Agustina; Zahrah Febianti; Enny Suswati; Diana Chusna Mueida; Muhammad Ali Shodikin; Samudra Ayu
Microbiology Indonesia Vol. 17 No. 2 (2023): June
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8013.23 KB) | DOI: 10.5454/mi.17.2.31-35

Abstract

Klebsiella pneumoniae develops antibiotic resistance by producing enzymes such as Extended-Spectrum Beta-Lactamase and Carbapenemase. Antibiotic resistance causes K. pneumoniae to have less antibiotic activity and more virulence factors. Capsule polysaccharides, lipopolysaccharide, Outer Membrane Protein, siderophores, and pili are all virulence factors in K. pneumoniae. This study aims to demonstrate the possibility of a host immunological response to the pili protein K. pneumoniae 65.5 kDa by injecting it into mice and measuring the levels of IFN-gamma cytokines in the mice's liver. This study used mice liver samples taken from 21 mice aged 6-8 weeks in the experimental investigation with a randomized post-test only controlled group design. Phosphate buffer saline was given to KI, pili protein antigen 65.5 kDa + Freunds' adjuvant was given to K2, and Freunds' adjuvant was given to K3. IFN-gamma concentration was measured using the sandwich ELISA method. The average concentration of IFN-gamma in the mice liver in this study was 247.68±47.67 pg m 'L ', 163.19±13.63 pg m'L', and 182.41 ±41.70 pg m'L'. The p-value of the Welch ANO VA test was 0.005 (p < 0.05), hence the Post Hoc Games-Howell test was used. The Games-Howell test showed a statistically significant difference in the mean value of IFN-gamma in KI compared to K2 and K3 of 0.007 and 0.046, respectively. There was no statistically significant difference between K2 and K3 with a p-value of 0.511. These findings revealed that intraperitoneal injection of Klebsiella pneumoniae pili protein 65.5 kDa did not increase IFN-gamma levels in the mice liver.
The Effect of Pili Protein of Klebsiella pneumoniae 65,5 kDa on Enhanced IFN- Gamma Levels in Mice Liver Dini Agustina
Microbiology Indonesia Vol. 17 No. 2 (2023): June
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.17.2.1

Abstract

Klebsiella pneumoniae develops antibiotic resistance by producing enzymes such as Extended-Spectrum Beta-Lactamase and Carbapenemase. Antibiotic resistance causes K. pneumoniae to have less antibiotic activity and more virulence factors. Capsule polysaccharides, lipopolysaccharide, Outer Membrane Protein, siderophores, and pili are all virulence factors in K. pneumoniae. This study aims to demonstrate the possibility of a host immunological response to the pili protein K. pneumoniae 65.5 kDa by injecting it into mice and measuring the levels of IFN-gamma cytokines in the mice's liver. This study used mice liver samples taken from 21 mice aged 6-8 weeks in the experimental investigation with a randomized post-test only controlled group design. Phosphate buffer saline was given to KI, pili protein antigen 65.5 kDa + Freunds' adjuvant was given to K2, and Freunds' adjuvant was given to K3. IFN-gamma concentration was measured using the sandwich ELISA method. The average concentration of IFN-gamma in the mice liver in this study was 247.68±47.67 pg m 'L ', 163.19±13.63 pg m'L', and 182.41 ±41.70 pg m'L'. The p-value of the Welch ANO VA test was 0.005 (p < 0.05), hence the Post Hoc Games-Howell test was used. The Games-Howell test showed a statistically significant difference in the mean value of IFN-gamma in KI compared to K2 and K3 of 0.007 and 0.046, respectively. There was no statistically significant difference between K2 and K3 with a p-value of 0.511. These findings revealed that intraperitoneal injection of Klebsiella pneumoniae pili protein 65.5 kDa did not increase IFN-gamma levels in the mice liver.
In Silico Study of Splicing Variations on Angiotensin-Converting Enzyme 2 (ACE2) and Its Effects on Infection from SARS-CoV-2 Ihsan Fauzan
Microbiology Indonesia Vol. 17 No. 2 (2023): June
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.17.2.6

Abstract

Several factors can influence SARS-CoV-2 infection. Alternative Splicing is thought to correlate with the differences of affinity value for interactions with the SARS-CoV-2 spike protein so that the infection may be influenced. This phenomenon can be happened by generating various kinds of protein expression variations with different isoform variants. So far, the difference in interaction affinity with the SARS-CoV-2 spike protein in Alternative Splicing has not been widely reported. Therefore, this study aimed to determine the isoform resulting from Alternative Splicing in the ACE2 transcript and its effect on SARS-CoV-2 infection. Molecular docking was used to determine the binding affinity between ACE2 proteins isoforms and SARS-CoV-2 spike protein. The 3D protein model of ACE2 isoforms obtained from the database was validated by evaluating the model quality of each ACE2 isoform. Based on docking results, there are variations in the docking scores of 8 isoform variants. However, there was no interaction between the ACE2_205 variant and SARS-CoV-2 spike protein while ACE2_206 and ACE2_207 showed a lower docking score than the other variants. In addition, essential residues in the interaction of each variant were also analyzed. Q42 and A384 are residues on the ACE2 protein that appear in interactions in more than two variants. These results indicate the possibility that splicing variations can cause differences in a person's level of susceptibility to SARS-CoV-2 infection, especially in the ACE2_205 variant that cannot interact with the SARS-CoV-2 spike protein.

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