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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 6 Documents
 1 
Search results for , issue "Vol. 15 No. 3 (2025)" : 6 Documents clear
The Physicochemical Properties of Butterfly Pea Flower (Clitoria ternatea L.) Extracts: Influence of Various Extraction Conditions Sitompul, Johannes Nathanael; Martati, Erryana
The Journal of Experimental Life Science Vol. 15 No. 3 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.03.01

Abstract

Butterfly pea flowers (Clitoria ternatea L.) have health benefits because they contain phenolic compounds, flavonoids, and anthocyanins, which act as antioxidants. There are few comparative studies on how the brewing temperature of butterfly pea flowers (hot, room, and cold temperatures) affects the release of antioxidants and anthocyanins. This study examined the effect of brewing methods on the physicochemical characteristics of butterfly pea flower extract. A nested experimental design was used, with brewing time nested within each brewing method. The brewing methods studied were hot brewing (HB) at 70°C for 5 and 15 minutes, room temperature (RT) brewing at 25-27°C for 3 and 6 hours, and cold brewing (CB) at 5-7°C for 12 and 24 hours. The color, pH, total anthocyanin content, and antioxidant activity were measured for the extract. The data obtained were statistically analyzed using ANOVA and Tukey's advanced test. The results showed that the brewing method significantly affected antioxidant activity but did not affect the total anthocyanin content, pH, or color. Brewing duration significantly influences all parameters except the color parameter b*. The best treatment of brewing methods for butterfly pea flowers was hot brewing for 15 minutes by simple additive weighting (SAW) method. The phenolic, flavonoid, alkaloid, and coumarin component groups made up the phytochemical composition of the butterfly pea flower extract.
Optimising Natural Bait Selection for Sustainable Handline Fishing: Behavioural Phase Analysis of Juvenile Barramundi (Lates calcarifer) in Response to Bait Type and Body Size Septiyani, Anita; Fuad, Fuad; Wiadnya, Dewa Gede Raka; Nurdiani, Rahmi; Hariati, Anik Martinah
The Journal of Experimental Life Science Vol. 15 No. 3 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.03.02

Abstract

Understanding the natural bait preference of predatory fish, such as barramundi, plays a crucial role in improving capture efficiency. This knowledge also supports sustainable fisheries by optimizing bait selection for handline fishing and reducing reef damage by active gear where barramundi species commonly live. This study examines the response of barramundi to various types of natural bait and investigates whether body size plays a significant role in this response. Predatory fish are divided into three different sizes (9, 13, and 15 cm total length), with each size having nine replicates. Fishing behavior and response to baits were divided into four phases: arousal, searching, finding, and uptake. Kinovea computer software (www.kinovea.org) was used to identify each phase with visual support from iVcam. The experiment was designed using a 54 L aquarium filled with saline water of 32 ppt. Data were analyzed using one-way ANOVA in SPSS version 26. When significant differences (p<0.05) were found, Tukey's post hoc test was performed. Bait from trash fish and shrimps did not show significant differences (p>0.05). Worms provided the fewest responses on times and behavior. Body size did not significantly affect behavioral responses or response times (p > 0.05). In conclusion, bait type significantly influenced barramundi's responses, with worms eliciting a significantly slower response compared to shrimp and trash fish, which were equally effective.
Changes in Blood Glucose and Cortisol in Nile Tilapia (Oreochromis niloticus) with Different Salinities Satiti, Cinca Hayyu Putri; Faqih, Abdul Rahem; Mahmudi, Mohammad; Moehammad, Khibar Syiar
The Journal of Experimental Life Science Vol. 15 No. 3 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.03.03

Abstract

Nile tilapia (Oreochromis niloticus) is a freshwater fish of high economic value that can tolerate a wide range of salinities, offering potential for the utilization of idle coastal ponds in northern Java. This study aimed to evaluate the physiological stress responses of Nile tilapia exposed to different salinity levels by measuring blood glucose levels, cortisol concentrations, and survival rates. The experiment was conducted for 28 days at the Sumberpasir Freshwater Fisheries Laboratory, Malang, using a Completely Randomized Design with four salinity treatments (0, 7, 14, and 21 ppt) in triplicate. Data on blood glucose and cortisol were analyzed using ANOVA (p < 0.05), while survival rate and water quality were analyzed descriptively. The results showed that salinity significantly affected blood glucose levels, with the highest value recorded at 21 ppt (115±39.2 mg.dL-1) and the lowest at 0 ppt (63±29.9 mg.dL-1). In contrast, cortisol levels were not significantly different among treatments, with the lowest average at 14 ppt (33.23±4.17 ng.mL-1). The highest survival rate was found at 0 and 7 ppt (100%), declining to 98% and 93% at 14 and 21 ppt, respectively. It is concluded that Nile tilapia can adapt physiologically to salinity levels of up to 21 ppt, with 14 ppt being the most stable level for stress adaptation. Further study is recommended to assess long-term growth and molecular indicators supporting the development of brackish water aquaculture.
Comparative Study of Terminalia catappa Linn. Leaf Extracts Against Aeromonas hydrophila Putra, Prily Lovian; Hariati, Anik Martinah; Prihanto, Asep Awaludin
The Journal of Experimental Life Science Vol. 15 No. 3 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.03.05

Abstract

Motile Aeromonas Septicemia (MAS), primarily caused by Aeromonas hydrophila, remains a critical threat in aquaculture due to its high mortality rates and economic impact. This study evaluated the antibacterial activity of crude Terminalia catappa L. leaf extract prepared using maceration and microwave-assisted extraction (MAE) methods against A. hydrophila in vitro. Phytochemical screening confirmed the presence of flavonoids, triterpenoids, tannins, and saponins in both extracts. The MIC test showed that MAE resulted in slightly lower optical density values than maceration, suggesting greater inhibition potential. Disk diffusion assays revealed that both extracts produced clear inhibition zones, with the MAE extract generating larger zones at equivalent concentrations. Scanning Electron Microscopy (SEM) confirmed morphological damage and lysis in A. hydrophila cells exposed to the extract. These findings demonstrate that T. catappa L. leaf extract exhibits significant antibacterial activity against A. hydrophila, with MAE being the more effective extraction method to enhance bioactivity.
The Influence of Shade Tree Diversity on Natural Enemy Communities and Microclimate in Coffee Agroforestry Systems Dewi, Nilasari; Kurnianto, Agung Sih; Haryadi, Nanang Tri; Rosita, Mitayuni Faur; Guretno, Titus Krido; Febrianti, Wanda Hamidah Zakiyah; Khowatini, Husnul; Priambodo, Bagus
The Journal of Experimental Life Science Vol. 15 No. 3 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.03.04

Abstract

Coffee agroforestry is an integrated farming system that combines coffee cultivation with the planting of shade trees. While it is well-established that vegetation diversity can support populations of natural enemies, few studies have investigated how varying levels of vegetation diversity interact with microclimatic conditions to influence insect community dynamics. Conducted in Desa Pace, Jember, East Java, the research compares simple and complex agroforestry systems in terms of their vegetation structure and the resulting impacts on natural enemy diversity. Field sampling was carried out in May, July, and September using yellow pan traps, accompanied by microclimatic measurements. Results revealed that the simple agroforestry had greater vegetation diversity. However, the Shannon-Wiener Index for vegetation was 0.94 in the complex system and 0.56 in the simple system, while the diversity of natural enemies was identical (1.70) in both systems. Microclimatic factors played a key role, with the simple having higher humidity and the complex exhibiting higher temperatures. These findings underscore that higher vegetation diversity does not always correspond to increased diversity among natural enemies, pointing to the influence of other ecological and environmental factors in shaping these communities. The study highlighted the importance of selecting appropriate shade vegetation to support sustainable and climate-resilient coffee agroforestry systems.
Mobile Phone Electromagnetic Waves and Pregnancy Outcomes: An Experimental Study in Mice Armalina, Desy; Susilaningsih , Neni; Sutanto, Heri; Sunarno, Sunarno
The Journal of Experimental Life Science Vol. 15 No. 3 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.03.06

Abstract

This study investigated the impact of mobile phone–derived electromagnetic wave (EMW) exposure on the uterus and progeny of pregnant Balb/c mice. Fourteen pregnant mice were randomly assigned to two groups (n=7 each). The control group (CG) was not exposed to EMW. In contrast, the experimental group (EG) was subjected to continuous exposure to EMW at a whole-body specific absorption rate (SAR) of 1.74 W.kg-1 for 24 hours daily from gestational day (GD) 1 to 19. On GD 20, all mice were euthanized, and body weight, oxidative stress biomarkers, and uterine histological parameters were assessed. Maternal body weight at termination showed a significant reduction in EG compared with CG (p < 0.05). However, differences in overall body weight gain, malondialdehyde (MDA), and superoxide dismutase (SOD) levels were not statistically significant (p > 0.05). Histological evaluation revealed marked alterations: endometrial scores were significantly higher in EG (3.32) than in CG (0.56) (p < 0.05), and myometrial scores were elevated in EG (1.72) versus CG (0.64). Endometrial thickness was reduced in EG (0.748 mm) compared with CG (1.470 mm) (p < 0.05), whereas myometrial thickness showed no significant difference. Continuous EMW exposure (24 h.day-1, GD1–GD19; SAR 1.74 W.kg-1) was associated with lower maternal weight at GD20, reduced litter size, higher endometrial/myometrial injury scores, and reduced endometrial thickness, while MDA and SOD differences were not statistically significant (p > 0.05). These findings warrant further studies to clarify the mechanisms involved without overstating the changes in oxidative biomarkers.

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