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Media Penelitian dan Pengembangan Kesehatan
Published by Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan
ISSN : 08539987     EISSN : 23383445     DOI : -
Core Subject : Health, Science,
Decision Sciences, Operations Research & Management Public Health
Media Health Research and Development ( Media of Health Research and Development ) is one of the journals published by the Agency for Health Research and Development ( National Institute of Health Research and Development ) , Ministry of Health of the Republic of Indonesia. This journal article is a form of research results , research reports and assessments / reviews related to the efforts of health in Indonesia . Media Research and Development of Health published 4 times a year and has been accredited Indonesian Institute of Sciences ( LIPI ) by Decree No. 396/AU2/P2MI/04/2012 . This journal was first published in March 1991.
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Search results for , issue "Vol 28 No 2 (2018)" : 10 Documents clear
Pengendalian Jentik Aedes sp. Melalui Pendekatan Keluarga Di Provinsi Papua Revi Rosavika Kinansi; Triwuri Sastuti; Zumrotus Sholichah
Media Penelitian dan Pengembangan Kesehatan Vol 28 No 2 (2018)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v28i2.120

Abstract

Dengue Hemorrhagic Fever has not been reduced yet, therefore, the Ministry of Health has conducted aRiset Khusus Vektor dan Reservoir in 2015 in 4 provinces, South Sumatera, Central Java, Central Sulawesiand Papua. This study aimed to determine the relationship of family participation in the community to thepresence of larvae Aedes sp. in Papua Province. Community attitudes to be studied include: the laying ofwater reservoirs, the closure of water reservoirs, the maintenance of fish in water reservoirs, the sowing oflarvicides at each water reservoir and the draining of water reservoirs. The presence of mosquito larvae isan indicator of the potential of community awareness of DHF. The sample in this analysis was 100 housesper district which was the Riset Khusus Vektor dan Reservoir 2015 area in Papua. The method of takinglarvae wass done by taking a larva or pupa Aedes sp. using plastic pipette and transferred into vial tubeusing Single Larvae Method technique. The results showed from the overall container placed in the house,28.27 percent were positive larvae. Non-drained containers had a 15 times positive chance of larvaecompared to containers that are diligently drained once a week. This study also showed that the resultsof keeping fish in containers have a ratio of no larvae, with larvae being 91: 9. Larvaside sowing had no real effect on the presence or absence of larvae in Papua Province. The role of families in the communityenvironment needs to be further improved and supported by the role of health workers in the prevention ofdengue hemorrhagic fever. Abstrak Adanya permasalahan penyakit tular vektor Demam Berdarah Dengue yang semakin lama tidak kunjung berkurang malah semakin bertambah, Kementerian Kesehatan RI melakukan studi Riset Khusus Vektor dan Reservoir pada 2015 di 4 provinsi, yaitu, Provinsi Sumatera Selatan, Jawa Tengah, Sulawesi Tengah dan Papua. Penelitian ini bertujuan untuk mengetahui hubungan peran serta atau perilaku masyarakat terhadap keberadaan jentik Aedes sp. di Provinsi Papua. Perilaku masyarakat yang akan diteliti antara lain: peletakan tempat penampungan air, penutupan tempat penampungan air, pemeliharaan ikan pada tempat penampungan air, penaburan larvisida pada setiap tempat penampungan air dan pengurasan tempat penampungan air. Keberadaan jentik nyamuk merupakan indikator dari potensi keterjangkitan masyarakat akan DBD. Sampel dalam analisis ini yaitu 100 rumah per kabupaten yang menjadi wilayah penelitian Riset Khusus Vektora 2015 di Propinsi Papua. Jika pada tempat penampungan air yang diperiksa ditemukan jentik atau pupa Aedes sp., maka diambil dengan menggunakan pipet plastik dan dipindahkan ke dalam tabung vial menggunakan teknik Single Larvae Method. Secara deskriptif, pada tahun 2015 keberadaan jentik di Provinsi Papua pada 2015 sebesar 31,5%. Jumlah tempat penampungan air yang diperiksa berjumlah 1355 kontainer. Sebesar 68,4 persen kontainer tidak ditemukan jentik dan 31,6 persen ditemukan jentik. Dari keseluruhan kontainer yang diletakkan di dalam rumah, 28,27 persen positif jentik. Kontainer yang tidak dikuras memiliki peluang 15 kali positif jentik dibandingkan dengan kontainer yang rajin dikuras seminggu sekali. Penelitian ini juga menunjukkan hasil memelihara ikan dalam kontainer memiliki rasio tidak terdapat jentik dengan terdapat jentik yaitu 91:9. Penaburan Larvasida tidak memiliki pengaruh nyata terhadap ada dan tidaknya jentik di Provinsi Papua.
Perilaku Berisiko dan Keluhan Subjektif Memori (KSM) pada Kelompok Umur 25 Tahun ke Atas di Kota Bogor Tengah Julianty Pradono
Media Penelitian dan Pengembangan Kesehatan Vol 28 No 2 (2018)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v28i2.122

Abstract

The prevalence of subjective cognitive complaints (SCC) is increasing over the last 10 years. Subjectivecognitive complaints reflect early signs of neurodegenerative disease that will develop into dementia. Theobjective of the study was to obtain the incidence rate of SCC and to get predictors of SCC in Bogor City.The sample was prospective cohort sample as much as 3260 respondents with mean age 47.6 ± 9.7 yearsand no SCC at the beginning of analysis. Data were collected by interview, measurement and laboratoryexamination every 2 years. The risk factors include characteristics, health status and risk behavior. Datawas analyzed using chi-square test and regression logistic. The incidence of SCC was 216 persons per1,000 population per 2 years. After adjusting for age, the SCC predictors were less physical activity (OR1.9 time; 95% CI: 1.6-2.3), central obesity (OR 1.2; 95% CI: 1.0-1, 5), intake of sodium ≥2000 mg per day(OR 0.6; 95% CI: 0,5-0,7), mental disorder (OR 1.9; 95% CI: 1.6-2.3 ) and migraine (OR 1.6 times, 95% CI:1.3-1.9) on the incidence of SCC. It is concluded that the incidence rate of SCC is 216 people per 1,000population per 2 years. Predictors of SCC are less physical activity, central obesity, intake of sodium ≥2000mg per day, mental disorders and migraine. It is suggested that specific interventions such as increasingphysical activity regularly, maintaining ideal body weight with no risk of abdominal obese, avoiding stressand controlling salt intake, especially in elderly respondents do not take too low intake. Abstrak Prevalensi keluhan subjektif memori (KSM) semakin meningkat.Keluhan subjektif memorimencerminkan tanda awal penyakit neurodegeneratif yang akan berkembang menjadi demensia. Tujuan penelitian ini untuk mendapatkan incidence rateKSM dan mendapatkan prediktor terjadinya KSM di Kota Bogor.Sampel penelitian merupakan subsampelkohor prospektif sebanyak 3260 responden dengan rerata umur 47,6±9,7 tahun dan bebas KSMdi awal analisis.Data dikumpulkan dengan metode wawancara, pengukuran, dan pemeriksaan yang dilakukan setiap 2 tahun.Faktor risiko meliputi karakteristik, status kesehatan dan perilaku berisiko.Analisis data dengan uji chi-square dan logistik regresi.Hasilpenelitian menyatakan insiden KSMsebesar 216 orang per 1.000 penduduk per 2 tahun.Setelah menyesuaikan faktor umur, prediktor KSM adalah kurang aktivitas fisik (OR 1,9 kali, 95% CI:1,6-2,3), obesitas sentral (OR 1,2 kali, 95% CI:1,0-1,5), asupan natrium ≥2000 mg per hari (OR 0,6 kali, 95% CI: 0,5-0,7), gangguan mental (OR 1,9 kali, 95% CI:1,6-2,3) dan migren(OR 1,6 kali, 95% CI:1,3-1,9) terhadap insidens KSM. Disimpulkan bahwa insiden KSM sebesar 216 orang per 1.000 penduduk per 2 tahun. Prediktor KSMadalah kurang aktivitas fisik, obesitas sentral, asupan natrium ≥2000 mg per hari, gangguan mental dan migren.Disarankan bahwa intervensi spesifik perlu dilakukan pada prediktor KSM.
Effect of Ethanolic Leaves Extract of Peperomia pellucida (L) Kunth as Antimalarial and Antioxidant Nanang Yunarto; Hanief Mulia Ar Rossyid; Lisa Andriani Lienggonegoro
Media Penelitian dan Pengembangan Kesehatan Vol 28 No 2 (2018)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v28i2.132

Abstract

In malaria infection, the Plasmodium parasites break down hemoglobin in the host's red blood cells into toxic free heme and amino acids. Plasmodium is also capable of converting free heme to non-toxic hemozoin through a polymerization process. Malaria infection, on the other hand, also triggers the formation of free radicals that affect the pathophysiology of malaria. Suruhan leaf (Peperomia pellucida (L.)Kunth) has long been used as an antimalarial and is known to contain large flavonoids that function as antioxidants. In this study, leaf extraction, inhibitory activity of heme polymerization assay, and antioxidant activity assay were performed. Organoleptically, leaf extract obtained in the form of dry blackish green coloured extractswith a bitter taste and the typical scent of suruhan leaves. The yield of the extract obtained was 18.28%, water content was 7.73%, drying shrinkage was 9.16%, and total ash content was 0.46%. The IC50 value of inhibition activity of heme polymerization of ethanol extract of the Suruhan leaves was 0.93 mg / mL, smaller compared to chloroquine(1.48 mg / mL)while the value of IC50. antioxidant activity of ethanol extract of leaf syrup was 32.94 mg / mL compared to Vitamin C of 4 mg/mL. In conclusion,ethanolic leaves extract of suruhan has inhibitory activity of heme polymerization and antioxidant activity so that it has potentiality as antimalarial drugs
Sistem Rujukan Maternal dan Neonatal di Daerah Kepulauan (Studi Kasus di Provinsi Papua dan Maluku) Heny Lestary; Sugiharti Sugiharti; Mujiati Mujiati
Media Penelitian dan Pengembangan Kesehatan Vol 28 No 2 (2018)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v28i2.177

Abstract

Maternal and neonatal health services are aimed to produce a healthy and quality generation and reduce maternal mortality rate (MMR) and infant mortality (IMR). MMR and IMR in Indonesia tend not to improve. Targeted reduction of MMR and IMR will be difficult to achieve if not given appropriate intervention, both socially, economically and culturally, as well as stabilization of referral system. In 2015, research on maternal and neonatal referral system in Papua and Maluku provinces was conducted. Data were collected at selected health centers, District hospitals, provincial hospitals, and regional referral hospitals. Study design is cross sectional. Data collection was done by interview, document review, verbal autopsy, and survey to health facilities and infrastructure. The results show that both provinces already have Governor Decree related to health care system and regionalization policy, but have not translated into Mayor Decree in each selected district. Referral flow indicates that there are still many health workers / families who choose directly to the district hospital or provincial hospital or health worker who refers to other closer regency hospitals. The number of maternal and neonatal deaths is still high, the unavailability of OBGY and Pediatricians, low compliance of the BEONC and CEONC standards. Consumables and drugs are often depleted because of the lack of coordination, uncontrolled stock, and the late of drug requests. Event though maternal and neonatal referrals have been financed through National Health Insurance, however still many shortcomings, both in terms of funding flows and problems in administrative completeness, as well as unavailability of accommodation and transportation costs for families and midwives who will accompany mother. Abstrak Pelayanan kesehatan maternal dan neonatal ditujukan untuk menjaga kesehatan ibu sehingga mampu melahirkan generasi yang sehat dan berkualitas serta mengurangi Angka Kematian Ibu (AKI) dan Angka Kematian Bayi (AKB). AKI dan AKB di Indonesia cenderung tidak mengalami perbaikan. Target penurunan AKI dan AKB akan sulit dicapai jika tidak diberikan intervensi yang tepat, baik secara sosial, ekonomi dan budaya, serta pemantapan sistem rujukan. Pada tahun 2015 dilakukan penelitian sistem rujukan maternal dan neonatal di Provinsi Papua dan Maluku. Pengumpulan data dilakukan di puskesmas terpilih, RSUD Kabupaten, RSUP Provinsi, dan RSUP Rujukan Regional. Disain penelitian studi potong lintang. Pengumpulan data dilakukan dengan wawancara, penelusuran dokumen, autopsi verbal, dan check list kelengkapan sarana dan prasarana. Hasil penelitian menunjukkan kedua provinsi sudah memiliki PerGub terkait dengan sistem pelayanan kesehatan dan kebijakan regionalisasi, namun belum diterjemahkan ke dalam PerBup di masing – masing kabupaten terpilih. Alur rujukan menunjukkan masih banyak tenaga kesehatan/keluarga yang memilih langsung ke RS kabupaten/RS provinsi atau tenaga kesehatan yang merujuk ke RS kabupaten lain yang lebih dekat. Jumlah kematian maternal dan neonatal masih tinggi, tidak tersedianya DSOG dan DSA, sarana prasarana masih belum sesuai standar PONED dan PONEK, ketersediaan dan kecukupan alat di Provinsi Papua masih di bawah 50 persen. Bahan habis pakai dan obat sering habis karena tingkat koordinasi, pengontrolan stok, dan daftar permintaan obat kurang terkontrol. Pembiayaan rujukan maternal dan neonatal melalui sistem JKN dan Jamkesda, namun banyak mengalami kekurangan, baik dalam hal alur pembiayaan maupun permasalahan di kelengkapan administrasi, serta tidak tersedianya biaya akomodasi dan transportasi bagi keluarga dan bidan pendamping pasien.
Kesahihan dan Keandalan Uji Jalan Enam Menit pada Lintasan 15 Meter Nury Nusdwinuringtyas; Idrus Alwi; Faisal Yunus
Media Penelitian dan Pengembangan Kesehatan Vol 28 No 2 (2018)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v28i2.178

Abstract

Walk test is a test that often used because walking is a basic activity. There are time-based walk tests and some are based on track length. A 6-minute walk test is a time-based walk test with a distance trevaled as a result. A 6-minute walk test is frequently used as functional clinical capacity trial. This test is performed on a straight track. The length of the track varies from 10-meters to 85-meters. This study examined the reliability and validity of a 6-minute walk test on a 15-meter track. The 15-meter track with markers every 3-meters, 30-centimeters wide to the right and left from the center line. The subject went straight as close to the center line as possible. When the subject arrived at both ends, the subject rotated with the three step method. The study compared the 6-minute walk test on the 15-meter track with three-step rotating method with on Biodex® gait trainer gold-standard. This study involved subjects as many as 123 healthy Indonesians adults (58 men, 65 women), and obtained Cronbach alpha of 0.999 and Pearson correlation value of 0.998. This shows that the results of the 15-meter trajectory test have validity and precision values which are as good as the 6-minute walk test without spinning. Abstark Uji jalan merupakan uji yang sering dipakai karena berjalan merupakan aktivitas dasar. Uji jalan ada yang berbasis waktu dan berbasis panjang lintasan. Uji jalan enam menit merupakan uji berbasis waktu dengan jarak tempuh sebagai hasil keluarannya. Uji jalan 6 menit merupakan uji klinis kapasitas fungsional yang sering dipergunakan. Uji ini dilakukan pada lintasan lurus. Panjang lintasan bervariasi dari 10 meter hingga 60 meter.. Penelitian ini meneliti keterandalan dan kesahihan uji jalan enam menit pada lintasan 15 meter. Lintasan sepanjang 15 meter dengan marka setiap tiga meter, lebar lintasan 30 sentimeter ke kanan dan ke kiri dari garis tengah. Subyek berjalan lurus sedekat mungkin dengan garis tengah lintasan. Ketika subyek tiba pada ke dua ujung , berbalik arah dengan metoda tiga langkah. Penelitian ini membandingkan uji jalan 6 menit pada lintasan 15 meter dengan metoda berputar pada ke dua ujungnya dengan baku emas uji jalan 6 menit pada Biodex®gait trainer. Melibatkan subyek 123 orang Indonesia dewasa sehat (58 laki-laki), didapatkan Cronbach alfa 0,999 dengan nilai korelasi r= 0,998. Hal ini memiliki arti bahwa hasil lintasan 15 meter memiliki kesahihan dan nilai ketepatan yang sama baiknya dengan uji jalan 6 menit.
Pemberdayaan Masyarakat Melalui Corporate Social Responsibility (CSR) dalam Pengendalian Penyakit Tidak Menular (Studi Agen Perubahan di Kecamatan Cicurug, Kabupaten Sukabumi) Rustika Rustika; Noor Edi Widy Sukoco; Tety Rachmawati
Media Penelitian dan Pengembangan Kesehatan Vol 28 No 2 (2018)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v28i2.179

Abstract

Number of hypertension cases in Cicurug, West Java, Health Center of Sukabumi District is 40% reported risk factors which are smoking habit, and clean healthy living behavior (PHBS) amounted 70%. Non communicable diseases control effort which is done by agent of change (AoC) through community empowerment. These activities could be held in collaboration with Corporate Social Responsibility (CSR). Operational research to analyze the implementation of CSR in community empowerment. Community empowerment is conducted in Nyangkowek Village (As Intervention Village) and Purwasari Village (As Control Village), Cicurug District, Sukabumi Regency. It has been selected 20 AoC from each village. Data were collected by in-depth interviews and observations. The AoC triggered has improved the knowledge, attitude and behavior of AoC in the control of NCD, they became promoters in controlling NCD risk factors for themselves, families, and community. Community empowerment through the selection of candidates and triggering AoC is the key to the successful implementation of P2TMBM. AoC as the spearhead in changing the risky behavior of NCD in the community that is coronary heart, hypertension, and obesity. CSR program in Cicurug District is able to develop community empowerment in controlling NCD risk factors. Health Office and Puskesmas are expected to be facilitators in forming AoC. Assistance by CSR is needed in the effort to control smoking behavior, diet and physical activity. Abstrak Jumlah kasus hipertensi di Puskesmas Cicurug Kabupaten Sukabumi sebesar 40%, dengan faktor risiko yang terlaporkan kebiasaan merokok, perilaku hidup bersih dan sehat (PHBS) sebesar 70%. Pengendalian penyakit tidak menular dilakukan melalui pemberdayaan masyarakat dengan pemicuan agen perubahan (agent of change). Tujuan penelitian untuk menerapkan program pemberdayaan masyarakat dalam pengendalian penyakit tidak menular berbasis masyarakat (P2PTM) melalui agen perubahan.Kegiatan tersebut terselenggara atas dukungan Corporate Social Responsibility (CSR) yang ada di Kabupaten Sukabumi. Metode penelitian adalah Riset operasional yang dilaksanakan dengan program CSR dalam pemberdayaan masyarakat. Tempat kegiatan di Desa Nyangkowek (intervensi pemicuan P2TMBM,) dan Desa Purwasari (kontrol).Penelitian ini dilakukan di bulan Februari- Oktober2015. Terpilih 20 agen perubahan dari masing-masing desa. Cara pengumpulan data dengan wawancara mendalam dan observasi. Hasil penelitian menunjukkan bahwa Pemicuan pada AOC telah berhasil meningkatkan pengetahuan, sikap dan perilaku Agent of Change dalam pengendalian penyakit tidak menular serta mereka menjadi promotor dalam pengendalian faktor risiko PTM baik untuk dirinya sendiri, keluarga maupun masyarakat sekitarnya. Pemberdayaaan masyarakat melalui pemicuan, pemilihan calon ‘Agen Perubahan’ serta dukungan CSR merupakan kunci keberhasilan program. Program CSR di Kecamatan Cicurug mampu mengembangkan pemberdayaan masyarakat dalam pengendalian faktor risiko PTM. Untuk keberlanjutan program pemberdaayan masyarakat dengan model pemicuan P2TMBM, diharapkan dinas kesehatan dan puskesmas dapat menjadi fasilitator dalam pembentukan ‘Agen Perubahan’ berikutnya, dalam upaya pengendalian perilaku merokok, pola makan dan aktifitas fisik dengan pendampingan oleh CSR.
Peran Indikator Pelayanan Kesehatan untuk Meningkatkan Nilai Sub Indeks Kesehatan Reproduksi dalam Indeks Pembangunan Kesehatan Masyarakat (IPKM) Lely Indrawati; Dwi Hapsari Tjandrarini
Media Penelitian dan Pengembangan Kesehatan Vol 28 No 2 (2018)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v28i2.180

Abstract

In Indonesia, measuring the success of development of a region is increasingly needed with the enactment of the Regional Autonomy System (OTDA). There are many methods or indicators that can be used as a measuring tool. The Public Health Development Index (IPKM) is one of the indicators that can be used to measure the success of community health development. This study aims to explore the correlation between several indicators of sub-index of Health Service (Yankes) and sub-index of Reproductive Health (Kespro) which become part of IPKM. Another purpose of this analysis is to find out which indicators are most leveraging for the Kespro sub-index. The method to analyse the data used Multiple Linear Regression with the district as the unit of analysis. According to the RISKESDAS 2013 data, there are 497 districts/cities in 33 provinces in Indonesia. RISKESDAS 2013 and Podes 2011 data are used by IPKM 2013. The results of the analysis show that the largest indicator giving the leverage of Kespro sub-index. That are the coverage of birth delivery by health worker in health facilities after controlled by the proportion of physicians per sub-district, the proportion of adequate posyandu per region and the health service coverage ownership (Jaminan Pelayanan Kesehatan/JPK) in each district. Abstrak Pengukuran keberhasilan pembangunan suatu daerah semakin dibutuhkan dengan berlakunya sistim Otonomi Daerah (Otda) di Indonesia. Banyak metode atau indikator yang dijadikan alat ukurnya. Untuk mengukur keberhasilan pembangunan kesehatan masyarakat, Indeks Pembangunan Kesehatan Masyarakat (IPKM) menjadi salah satu indikator yang digunakan. Penelitian ini bertujuan menggali hubungan beberapa indikator pembentuk sub indeks Pelayanan Kesehatan (Yankes) terhadap sub indeks Kesehatan Reproduksi (Kespro) yang menjadi bagian dari IPKM. Tujuan lain dari analisa ini yakni menggali indikator mana yang paling memberi efek ungkit bagi sub indeks Kespro. Metode analisa yang digunakan adalah Regresi Linier Berganda dengan kabupaten sebagai unit analisanya. Terdapat 497 kabupaten di 33 provinsi di Indonesia sesuai dengan jumlah kabupaten pada saat Riset Kesehatan Dasar (Riskesdas) dikumpulkan pada tahun 2013. Riskesdas 2013 dan Podes 2011 menjadi sumber data yang digunakan IPKM 2013. Hasil dari analisa didapat indikator yang paling besar memberikan daya ungkit sub indeks Kespro yakni cakupan persalinan ditolong oleh tenaga kesehatan di fasilitas kesehatan setelah dikontrol proporsi jumlah dokter per kecamatan, proporsi jumlah posyandu per desa dan kepemilikan Jaminan Pelayanan Kesehatan (JPK) di setiap kabupaten.
Konstruksi Plasmid Rekombinan untuk Inisiasi Translasi Enhance Green Fluorescent Protein oleh Internal Ribosomal Entry Site HIV-1 Cintera Rahmagiarti; Silvia Tri Widyaningtyas; Budiman Bela
Media Penelitian dan Pengembangan Kesehatan Vol 28 No 2 (2018)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v28i2.181

Abstract

Human immunodeficiency virus (HIV) is a virus that causes acquired immunodeficiency virus syndrome (AIDS). The HIV genome has a cap structure at 5’ and polyadenylation at 3’ on mRNA resulting in a translation initiation through scanning at 5'untranslated region (UTR). The Vpr protein produced during viral replication causes the 5'cap scanning to be inhibited so HIV-1 can directly recruit the ribosome at the start codon via internal ribosomal entry site (IRES). IRES activity is high at G2/M phase and highest expression in monocyte cell line (THP-1) and lymphocyte (HPB-ALL). The role of HIV IRES however, is not yet known in infection of nondividing cells by HIV-1. HIV-1 IRES and egfp from pcDNA5FRT/TO were amplified with PCR. The insert DNA (HIV-1 IRES_egfp) and pcDNA3.1(+) were digested with EcoRI and ApaI and then ligated. The verification was performed with PCR colonies, restriction verification, and sequencing. The size of insert DNA is 1067 bp while the vector is 5379 bp. E. coli transformed with DNA ligation produces 70 colonies, control of ligation produces 5 colonies, and negative control didn’t grow. 19 colonies contain recombinant DNA, restriction verification was of the appropriate size, and the sequence verification didn’t find any mutation. Therefore, the subcloning process pcDNA3.1_IRES HIV-1_egfp was successfully performed. Abstrak Human immunodeficiency virus (HIV) merupakan virus penyebab acquired immunodeficiency virus syndrome (AIDS). Genom HIV memiliki struktur cap di 5’ dan poliadenilasi di 3’ mRNA sehingga proses inisiasi translasi melalui pemindaian 5’cap pada struktur untranslated region (UTR) di 5’ mRNA HIV. Protein Vpr yang dihasilkan selama replikasi virus menyebabkan pemindaian melalui 5’cap terhambat sehingga HIV-1 dapat langsung merekrut ribosom pada kodon awal translasi melalui struktur internal ribosomal entry site (IRES). Aktivitas IRES tinggi pada fase G2/M dan ekspresi gen tinggi pada sel line monosit (THP-1) dan limfosit (HPB-ALL). Namun, peran IRES HIV-1 belum diketahui pada sel tidak membelah yang merupakan sel target pada infeksi HIV-1. DNA sekuen IRES HIV-1 dan egfp dari pcDNA5FRT/TO diamplifikasi dengan PCR. DNA sisipan (IRES HIV-1_egfp) dan pcDNA3.1(+) dipotong dengan EcoRI dan ApaI lalu DNA sisipan diligasi dengan pcDNA3.1(+). Verifikasi hasil klona dilakukan dengan PCR koloni, verifikasi restriksi, dan sekuensing. Restriksi DNA sisipan menghasilkan pita berukuran 1067 pb. Restriksi vektor plasmid menghasilkan pita berukuran 5379 pb. E.coli yang ditransformasi DNA ligasi menghasilkan 70 koloni, kontrol ligasi 5 koloni, dan kontrol negatif tidak tumbuh. 19 koloni terverifikasi mengandung DNA rekombinan, verifikasi restriksi memiliki ukuran sesuai, dan verifikasi sekuensing tidak terdapat perubahan basa. Oleh karena itu, proses subkloning pcDNA3.1_IRES HIV-1_egfp berhasil dilakukan.
Front Matter Media Vol 28 no 2
Media Penelitian dan Pengembangan Kesehatan Vol 28 No 2 (2018)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v28i2.642

Abstract

Back Matter Media Vol28 No 2
Media Penelitian dan Pengembangan Kesehatan Vol 28 No 2 (2018)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v28i2.643

Abstract

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