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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Published by Universitas Gadjah Mada

ISSN : 01261312 EISSN : 23563931 DOI : http://dx.doi.org/10.19106/JMedSci005303202101

Core Subject : Science,

Immunology & microbiology Neuroscience

Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia

Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)

Articles 34 Documents

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Search results for , issue "Vol 44, No 01 (2012)" : 34 Documents clear

The correlation between TAM,MVD, VEGF andMMP-9 expressions among various histological progression, histological grading and staging of breast cancer Angelina Septiana, Brian Arditya M, Irianiwati, Harijadi Surendra Prabawa, Yulius Hermanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Breast cancer may progress from an atypical lesion. Angiogenesis has an important role in thegrowth, invasion and metastasis of breast cancer, which can be reflected through the microvasculardensity (MVD). Tumor associated macrophages (TAMs) are stromal cells that can produce proangiogenicfactor such as vascular endothelial growth factor (VEGF), and induce matrixmetalloproteinase-9 (MMP-9) for degrading extracellular matrix and basement membrane.Angiogenesis in breast cancer progression and its relationship with histological grading as wellas its staging need to be defined and thus, cancer therapy and prognosis can be determinedmore accurately. The aim of this study was to investigate the correlation between TAM, MVD,VEGF and MMP-9 expressions among fibrocystic lesion, atypical lesion and breast cancer, andits correlation with histological grading and staging of breast cancer. Using a cross-sectionalstudy, a total of 50 paraffin embedded tissues of fibrocystic lesion, atypical lesion and breastcancer were chosen in this study. Those specimens were stained immunohistochemically withmonoclonal antibody (MoAb) anti CD68, von Willebrand factor (vWF), VEGF and MMP-9. Theexpressions of VEGF and MMP-9 were counted from the mean numbers of positive tumor cells.TAMs were counted from numbers of macrophages which expressed CD-68. MVDs were countedfrom numbers of microvessels whose endothelial cells expressed vWF, using the AverageMicrovessels Count ( AMC) method. The correlation of both markers and different type of breastlesions were analyzed by using Pearson correlation. There were statistically significant correlationsbetween TAM (r=0.760; p=0.000), MVD (r=0.659; p=0.000), and MMP-9 (r=0.518; p=0.000),among several breast lesions and histological grade of breast cancer. The highest of theirexpressions was found in the poor grade of cancers. There were statistically significant correlationsbetween TAM (r=0.581; p=0.000), VEGF (r=0.443; p=0.001) and MVD (r=0.566; p=0.000)among fibrocystic, atypical lesion and stage II - III of breast cancer. VEGF expression was notsignificantly correlated with several histological grade of breast cancer and the highest of itsexpression was only found in atypical lesion. This study has suggested that TAM, MVD, VEGFand MMP-9 expressions might play an important role in the histological progression, histologicalgrading and staging of breast cancer. The highest expression of VEGF in atypical breast lesionsupported the fact that angiogenic switch already started in the early stage and grade of breastcancer.

Effect of swimming and asthmatic exercise on forced expiratory volume in 1 second (FEV1) and levels of cortisol hormone in asthmatics patients Achmad Djunaedi, Rahmaya Nova Handayani, Denny Agustiningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Bronchial asthma is one of the allergic diseases characterized by reversible bronchial narrowing dueto bronchial hyperactivity and obstruction of respiratory tract. The prevalence and hospitalizationrates of bronchial asthma keep increasing from year to year, causing asthma to be the top ten causesof morbidity and mortality in Indonesia. The decrease of the forced expiratory volume in 1 second(FEV1) is one of the indicators of the respiratory tract obstruction. Moreover, the cortisol hormonelevel is also associated with asthma. Asthma can be treated not only with pharmacological interventionbut also physical exercises. The aim of the study was to assess the increase of FEV1 and cortisolhormone level after swimming and asthmatic exercise on asthmatic patients. This was a quasiexperimental study using pre and post test control group design. Two groups of 10 asthmaticpatients each conducted swimming and asthmatic exercise as intervention. On pre, middle and postswimming or asthmatic exercise, those two groups underwent the measurement of FEV1 using aspirometer and cortisol hormone levels using ELISA methods. The result showed that the value ofFEV1 and cortisol hormone levels increased significantly after swimming and asthmatic exercise(p<0.05). Moreover, the improvement of FEV1 value and cortisol hormone levels of asthmaticpatients in swimming were higher than asthmatic exercise. In conclusion, swimming and asthmaticexercise can increase the value of FEV1 and the levels of the cortisol hormone.Keywords : swimming - asthmatic exercise - FEV1 - cortisol hormone - asthma

Activity of ï¡-terpineol as a potential anticancer candidate: cytotoxicity, proapoptotic and antiproliferative evaluation in TD47 cell lines Mustofa, Puguh Indrasetiawan Indwiani Astuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Previous study has shown that ï¡-terpineol has a cytotoxic effect on NCI-H69 cancer cell line.This study aimed to evaluate cytotoxicity, proapoptotic, and antiproliferative activities of ï¡-terpineol in T47D cell lines. The cytotoxic activity of ï¡-terpineol was determined using MTT cellviability assay, while apoptosis was tested by the acridine orange-ethidium bromide stainingmethod. The cell cycle was evaluated by flowcytometry method. The results showed that ï¡-terpineol had cytotoxic effect on the T47D cells with the IC50 value of 135.00 Â± 8.74 ïM.Furthermore, ï¡-terpineol induced the cells apoptosis in a dose-dependent manner. Flowcytometryanalysis showed that ï¡-terpineol induced the T47D cell distribution in Go/G1 phase, howeverinhibited cell distribution in S phase. This study suggests that ï¡-terpineol has a potential anticanceractivity.

Targeted phototherapy for skin diseases Arief Budiyanto, SaÃ¢Â€Â™adatul Huriyah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Universitas Gadjah Mada

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Phototherapy is a therapeutic strategy in dermatology for treating several skin diseases.Conventional phototherapy has some disadvantages, hence a targeted phototherapy that emitsselective laser or ultraviolet (UV) light and targets specific area of affacted skin has been developed.Targeted phototherapy is considered to be more aggressive and has higher efficacy. Severaltargeted phototherapy devices emit one or several types of light. The use of targeted phototherapyhas been studied in vitiligo, psoriasis, eczema, keloid, hypopigmented scar or striae alba, andoral lichen planus.

The droplet size changes of 1% propofol before and after the storage procedure for 6 and 24 hours periods Djayanti Sari, Basuki Rahmat Bambang Suryono S
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Universitas Gadjah Mada

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Propofol (2,6-diisopropylphenol) is a popular anesthetic agent for induction and anestheticmaintenance. Propofol preparation is formulated as a lipid macroemulsion that is thermodynamicallyunstable and degraded over time, causing the enlargement of droplet size. The degradation is fasterwhen propofol emulsion preparation package has been opened. This droplet enlargement results indecreasing propofol releasing-rate and its concentration. The risk of embolism occurs whenever themean droplet size (MDS) exceeds Food and Drug Administration (FDA) limit (<0.45) and fat globulepopulation percentage >5 ïm (PFAT5) is <0.05%. In the daily practice, some propofol emulsionpreparation are often used although they are already opened and saved more than 24 hours. The aimof this study was to evaluate the droplet size changes of propofol emulsion preparation duringstorage. This was an observational study using cohort prospective design. The droplet size changesof sixteen propofol preparation samples were evaluated before and after storage procedure for 6 and24 hours in the refrigerator at the temperature of 4ÂºC. The droplet diameter was determined under alight microscope. Mean droplet size before and after storage were calculated and analyzed by oneway analysis of variance (ANOVA), followed by Tukeyâs post hoc test with 95% confidence interval(p<0.05). The droplet diameter of propofol emulsion preparation increased significantly (p<0.05)after storage procedure for 6 h (MDS value were: 247 Â± 22 nm) and 24 h (278 Â± 29 nm) comparedto before storage (225 Â± 24 nm). The PFAT5 at each interval time was 0%. There was no color andhomogeneity changing at each interval time (n=16/100% each time interval). In conclusion, there isa change of propofol emulsion preparation droplet size after storage procedure for 6 to 24 hours attemperature of 4ÂºC compared to before storage.

Antenatal and postnatal risk factors of obesity in children age 2-5 years old in Yogyakarta District, Indonesia Yudha Patria, I Ketut Rutin Pastadita Sunartini
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Universitas Gadjah Mada

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Obesity and overweight have become a health issue throughout the world, with a dramaticincrease in prevalence each year. An obese child is thought to be adorable, but parents are notaware of the health risk it holds. Preventive measures by decreasing the risk factors for obesityare more effective than treating the problem. The aim of this study was to identify the riskfactors of obesity in antenatal and postnatal period in children in Yogyakarta District. This wasan observational study with a case control design. Criteria of obesity were defined according toWHO 2006 growth chart. Subjects were children age 2 to 5 years old that were enrolled inplaygroups in the Yogyakarta Distric with a targeted sample size of 41 children. Parents ofchildren that met the inclusion and exclusion criteria were given questionnaires to fill out. Theresults were analyzed using bivariate analysis and multivariate logistic regression. Thirty fourobese children and 34 non obese children (as control) were explored for the obesity risk factors.Prematurity/gestational age (OR=16; 95%CI=3.27-78.28; p=0.001), passive smoking (OR=5.50;95%CI=1.90-15.96; p=0.001), low birth weight (OR=7.27; 95%CI=1.45-36.47; p=0.008),eating more than 3 times daily (OR=2.19; 95%CI=1.66-2.89; p=0.007), meals that are high inlipid (OR=4.18; 95%CI=1.45-12.02; p=0.007) were shown to be risk factors for obesity inchildren. Factors such as picky eaters (OR=0.16; 95%CI=1.45-12.02; p=0.002), early introductionto solid food (OR=0.29; 95%CI=0.10-0.79; p=0.01), exercise (OR=0.25; 95%CI=0.06-0.32;p=0.04), and watching TV or playing video game under 2 hours daily (OR=0.30; 95%CI=0.09-0.95; p=0.04) were identified as protective factors against obesity. By using the multivariateanalysis, there were only 2 risk factors for obesity i.e. eating more than 3 times daily andprematurity/gestational age and there were only 2 protective factors i.e. exercise and introductionto solid food that remained statistically significant as independent factors influencing obesity inchildren aged 2 to 5 years old in the Yogyakarta District.Keywords: obesity - children age 2 to 5 years old - antenatal - postnatal - risk factors

Serum testosterone level and active caspase-3 of Leydig cells of diabetic Sprague-Dawley male rats after administration of soybean (Glycin max) powder suspension Sri Kadarsih Soejono, Wiyadi Dicky Moch. Rizal
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Universitas Gadjah Mada

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Diabetes mellitus (DM) is known as the main death cause in the world. This disease causes acuteand chronic complication. The common chronic complication on male reproduction system isthe decrease of testosterone level. This hormone is produced in the Leydig cells. Soybeans(Glycin max) has been used in the management of DM to maintain blood glucose level. However,the effect of soybean on serum testosterone level is still unclear. The aim of this study was toevaluate serum testosterone level and caspase-3 active of Leydig cells of diabetic Sprague-Dawley (SD) male rats model induced by streptozotocine (STZ) after oral ingestion of soybeanpowder suspension. This was an experimental study with pre and post test control group design.Thirty SD male rats, aged 11-12 weeks with body weight (BW) of 200-250 g were divided into 5groups with 6 rats in each group. The first group (G1) was normal rat control and the secondgroup (G2) was diabetic rat control. The third to fifth group (G3, G4 and G5) were diabetic rattreatment with oral ingestion of soybean powder suspension that was given once a day during 4weeks with dose of 400; 800 and 1600 mg/kg BW, respectively. The serum testosterone levelwas measured by an ELISA and active caspase-3 of Leydig cells were measured by animmunohistochemistry method. The result showed that the serum testosterone level and activecasapse-3 Leydig cells of diabetic rats model induced by STZ were not significantly differentafter oral ingestion of soybean powder suspension with dose of 400, 800 and 1600 mg/kg BW(p>0.05). In conclusion, soybean powder suspension did not affect the serum testosteronelevel and active caspase-3 Leydig cells in diabetic rats.Keywords: diabetes - soybean - phytoestrogen - testosterone - active caspase-3

Effect of swimming and asthmatic exercise on forced expiratory volume in 1 second (FEV1) and levels of cortisol hormone in asthmatics patients Rahmaya Nova Handayani, Denny Agustiningsih Achmad Djunaedi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Activity of -terpineol as a potential anticancer candidate: cytotoxicity, proapoptotic and antiproliferative evaluation in TD47 cell lines Puguh Indrasetiawan Indwiani Astuti Mustofa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6440.112 KB)

Previous study has shown that -terpineol has a cytotoxic effect on NCI-H69 cancer cell line.This study aimed to evaluate cytotoxicity, proapoptotic, and antiproliferative activities of -terpineol in T47D cell lines. The cytotoxic activity of -terpineol was determined using MTT cellviability assay, while apoptosis was tested by the acridine orange-ethidium bromide stainingmethod. The cell cycle was evaluated by flowcytometry method. The results showed that -terpineol had cytotoxic effect on the T47D cells with the IC50 value of 135.00 ± 8.74 M.Furthermore, -terpineol induced the cells apoptosis in a dose-dependent manner. Flowcytometryanalysis showed that -terpineol induced the T47D cell distribution in Go/G1 phase, howeverinhibited cell distribution in S phase. This study suggests that -terpineol has a potential anticanceractivity.

Targeted phototherapy for skin diseases Sa’adatul Huriyah Arief Budiyanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Nurhadiyahya

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Kab. sleman,

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