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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

biota
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Published by Universitas Atma Jaya Yogyakarta

ISSN : 25273221 EISSN : 2527323X DOI : doi.org/10.24002/biota

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 18 Documents

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Search results for , issue "Vol 11, No 2 (2006): June 2006" : 18 Documents clear

Aktivitas Harian Bekantan (Nasalis larvatus Wurmb) di Hutan Karet Kabupaten Tabalong, Kalimantan Selatan Soendjoto, Mochamad Arief; Alikodra, Hadi Sukadi; Bismark, Muhammad; Setijanto, Heru
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

The daily activities of the proboscis monkey (Nasalis larvatus Wurmb) living inrubber forests are poorly understood. The objectives of the research were to studyits daily movement pattern and to determine the time proportion of daily behaviors.The movement of the monkeys was followed by researchers on foot. The behaviorswere observed by scan technique in 15 second intervals for 30 minutes. In rubberforests, lowlands (baruh) were places where the monkey began and ended dailymovements and also conducted most activities. Most inter-water movements of themonkey (about 70%) were conducted twice a day. Statistically, movement distanceand rainfall were negatively and significantly correlated (Ò‘s = -0.58; p < 0.05; n = 14).The monkey spent more time for feeding in the morning and afternoon, and restingat midday. Most behaviors of feeding, resting, playing, and grooming (84.01%) wereconducted at a tree height of less than 15 m. The monkeys moved farther during thedry season to get water and did activities in accordance with height strata to getvarious foods, avoided disturbances, and got comfortable and safe places.

The Reproductive Patterns of Two South Australian Mytilid Species: Brachidontes erosus (Lamarck, 1819) and Brachidontes rostratus (Dunker, 1857). Ompi, Medy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Pola reproduksi dari dua jenis Kerang, Brachidontes erosus dan Brachidontes rostratus di perairan laut Port Lincoln, Australia Selatan, Australia ditentukan berdasarkan ukuran telur, pola settlement selama 18 bulan, dan juga aktivitas pemijahan di laboratorium. Gamet dari B. erosus nampak mulai berkembang pada bulan Februari dan mencapai puncak pada bulan September, sekaligus sebagai awal pemijahan, yang mencapai klimaks pada bulan November sampai bulan Januari. Jenis B. rostratus nampak memiliki dua puncak matang gonad, yang satu terjadi di bulan Juli dan puncak matang gonad kedua terjadi pada bulan November sampai Februari. Puncak pemijahan dari jenis ini berada di bulan Februari sampai dengan April. Kerang B. erosus memiliki ukuran diameter telur lebih besar dibandingkan dengan jenis kerang B. rostratus. Faktor-faktor seperti makanan, suhu perairan, karakter kerang dan kondisi perairan lainnya kemungkinan mempengaruhi pola reproduksi kedua jenis kerang ini.

Produksi Fitoaleksin pada Tusam (Pinus merkusii Jungh. et de Vriese) sebagai Respon Infeksi Fungi Mikorisa Widyaningsih, S.; Widyastuti, S. M.; Sumardi, Sumardi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

The experiment aimed to detect (1) mycorrhizal infection induced phytoalexins production of the root of tusam and (2) its activity in suppressing a dumping off fungi, Fusarium sp and Rhizoctonia solani. Production of phytoalexins was detected from the extracts of mycorrhizal root of old trees and 4, 6 and 8 week-old seedlings in alcohol using a UV-spectrophotometer. The antifungal activity of phytoalexins was studied by inoculating the pathogenic fungi into mycorrhizal seedlings of tusam. The results showed that the spectra of phytoalexins of extracted mycorrhizal and non-mycorrhizal roots ranged from 203.2 to 204.6 nm. Higher antifungal concentrations of antifungal compound was obtained from the root extracts of old trees and 4 week-old seedlings, indicated by the higher spectrophotometric absorbance (0.315 and 0.324 g-1 root ml-1 ethanol), compared to that extracted from non-mycorrhizal root of the same plant origin. Mycorrhizal roots significantly suppressed the development of Fusarium sp. and Rhizoctonia solani.

Notes of Honey Buzzard and Other Birds in Dumai and Rupat Island, Riau Province, Indonesia Sukmantoro, Wishnu; Cye, Lim Kim; Tiah, Lim Au; Iqbal, Mohammad; Ng, Francis
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Dumai are a town that located coastal area in the Nothern part of Pekan Baru, a capital city of Riau Province (1o 41â€™ 04.1â€ N, 101o 26â€™ 14,1â€ E). Dumai is margined directly by Rupat Island and Bengkalis Island in the North and Duri District in the South and East. On February 17, survey is conducted in Dumai harbour which vegetation are dominated rural plant and small scale mangrove. Only few records of bird are reported on 1980 such as storm stork Ciconia stormi (Holmes 1980).

Perilaku Spasio Temporal Tikus Sawah (Rattus argentiventer) Betina Hadi, Susilo; Subagja, Jusup; Sudarmaji, Sudarmaji
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

This research aimed to study spatio temporal behavior of the female ricefield rat, Rattus argentiventer, including home range, range spans, habitat use and daily activity. Twelve rats were released in two locations of a ricefield area from June 11th until July 24th, 2001. The movement of each rat was monitored using radio-tracking three times a day. The result was used for calculating home range size, range spans, habitat used and daily activities of female field-rats, using the computer program Ranges V. The results showed that the average female ricefield-ratâ€™s home ranges and range spans were 0.84 ï‚± 0.16 Ha and 184.54 ï‚± 22.7m respectively. During daylight, the rats spent 82%, 16% and 2% of their activity living in dikes, in villages and in ricefield respectively. On the contrary, during the night 95%, 3% and 2% of the rats were active in the ricefield, villages and dikes respectively.

Observations on Natural Foods and Nutrition Content of Critically Endangered Turtle (Leucocephalon yuwonoi) in Central Sulawesi Riyanto, Awal; Wirdateti, Wirdateti; Soemarno, Suprayogo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Kura-kura Sulawesi (Leucocephalon yuwonoi) adalah satu dari dua jenis kura-kura endemik Sulawesi dengan penyebaran yang terbatas. Sejauh ini data natural historinya sangat minim, padahal sangat dibutuhkan dalam upaya penangkaran satwa yang berstatus kritis IUCN ini. Penelitian ini bertujuan untuk mengungkap tentang pakan di alam beserta kandungan nutrisinya. Survei lapangan dilakukan di kawasan Bangkir dari tanggal 23 Mei sampai 5 Juni 2004 dan di Moutong dari tanggal 11 sampai 19 Oktober 2004. Data pakan di alam diperoleh dari analisis sampel feces dan hasil wawancara kepada para pemburu kura-kura beserta pengumpul. Kepastian nama jenis pakan diperoleh dengan melakukan identifikasi sampel tumbuhan pakan di Herbarium Bogoriense. Data kandungan nutrisi diperoleh melalui analisis proksimat sampel pakan yang terkoleksi selama survei. Hasil penelitian ini menunjukkan bahwa terdapat 32 jenis tumbuhan yang jadi sumber pakan di alam, variasi kandungan nutrisi yang cukup besar, kandungan lemak rendah berkisar antara 0.74 - 8.33% (2.41+2.03) dari berat kering. Berdasarkan dominansi, keberadaan di habitat (multiple season) dan tingginya kandungan energi diduga kuat bahwa Colocasia esculenta, Limnocharis flava and Ipomoea aquatica merupakan pakan utama di alam.

KUALITAS, KEMAMPUAN IMPLANTASI DAN VIABILITAS IN-VIVO EMBRIO MENCIT (MUS MUCULUS) GALUR SWISS WEBSTER SETELAH PEMBEKUAN DENGAN METODE VITRIFIKASI Madihah, Madihah; Kusumaningtyas, Hartanti; Boediono, Arief; Sumarsono, Sony H.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Reproductive technologies including in vitro fertilization (IVF), embryo manipulation, gamete and embryo freezing, thawing and embryo transfer were rapidly developed. Vitrification is an embryo freezing technique that is the most developed. In this experiment, we vitrified mouse embryos and then examined the embryos i.e: (i) the quality of the embryos after thawing, (ii) the implantation rate of the embryos and (iii) viability of the embryos in vivo. Morulae and blastocycsts were collected from female mice that were pregnant a day 3,5. The embryos were equilibraten in mPBS +10% etilene glycol. Vitrification was carried out by using VABEDS medium, containing 6-10 embryos that were dropped into a tip of a straw, then frozen in liquid nitrogen for 24 hours. Thawing was carried out by flushing the embryos using mPBS suplemented with 0.5, 0.25, 0.1 and 0 M sucrose. After being incubated in M2 medium at 37oC for 1-2 hours, the recovery embryos were then transferred into the uteri of day 2.5 of pseudopregnat females. The females were then sacrificed at day 16 of gestation and the total implantaion, total life and death fetuses, as well as resorpted embryos, were taken as data. The results showed that vitrification significantly (p<0,05) reduced the quality of the embryos, as well as their implantation rate and the viability of the fetuses, which may be caused by the unoptimal combination of the cryoprotectant in the vitrification medium, temperature and exposure time during vitrification.

Purifikasi dan Karakterisasi Krom Reduktase Bacillus sp LKA9 Ali, Alimuddin; Soetarto, Endang Sutariningsih; Widada, J. Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Chromate reductase is one of the potential enzymes for hexavalent chrom detoxification. Most of the enzyme is produced by bacteria, especially Bacillus. The aim of this research was to study chromate reductase activity isolated from Bacillus sp LKA9. Bacillus sp LKA9 was isolated from leather tannery liquid waste and used as a model in the experiment. Bacillus sp LKA9 was isolated through enrichment culture using Salt Base Solution containing 3 mM K2CrO4. Chromate reductase was isolated from bacteria by growing on a liquid medium containing chrom hexavalen (Cr VI) through several steps. The first step of the isolation process was to use the precipitated process using ammonium sulphate (30-80%). The next step, crude enzymes from the first step was partially purified through DEAE-Cellulose of Ion Exchange Chromatography Column. Diphenylcarbazide methods was used to examine the activity of enzyme fractions. The result of the experiment revealed that all protein could be precipitated by ammonium sulphate, and the cytoplasmic fraction at saturation of 50-70% showed high enzyme activity. Purified enzymes showed an increase activity 69,385 times to that of crude enzymes. The enzyme optimal had temperature and pH were 350C and 5; respectively. KM of enzyme was 0,0075 mM, and Vmax was 2500 ïmol/minute/mg protein. Enzyme activity was not inhibited by Cu2+, but an ion Ag2+ and Hg2+ inhibited the enzyme activity un-competitive. The activity of enzyme was demonstrated on SDS-PAGE by appearing typically band with molecular weight 29,26 kDa, it was assumed as chromate reductase.

Pengaruh Heat-Moisture Treatment dan Penambahan Natrium Tripolifosfat terhadap Kualitas Mi instan dari Pati Ubi Jalar (Ipomoea batatas (L) Lam) Suryani, Chatarina Lilis
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.2615

The objective of the present study was to evaluate the effect of heat-moisturetreatment (HMT) and sodium tripolyphosphate (STPP) addition on the quality ofinstant noodles from sweet potato starch. The diversification of raw material used innoodle production often necessitates processing modifications. Heat-moisturetreatment was applied to native sweet potato starch (HMTSPS) in three conditions(1100C; 10, 20 and 30 min) and STPP was added in three level (0,60; 0,75 and 0,90%).HMTSPS, STPP, water, lecitin and CMC were mixed. The mixture was pelleted,steamed and extruded. The extruded noodles were dried using a microwave oven. Thenoodles were evaluated both when dry and after cooking. The HMT method andSTPP addition improved noodle quality. Swelling index, solid loss and cooking loss ofthe noodles was decreased, while the texture and the elastisity of the noodles washigher. But noodles made with added of STTP more than 0,75% had low elastisity.The best instant noodle prepared by HMT method for 20 min and added of STTP0,75%.

Pengaruh Heat-Moisture Treatment dan Penambahan Natrium Tripolifosfat terhadap Kualitas Mi instan dari Pati Ubi Jalar (Ipomoea batatas (L) Lam) Chatarina Lilis Suryani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.2615

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Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

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+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

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Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Brigitta Laksmi Paramita

Contact Email brigitta.laksmi@uajy.ac.id

Phone +6282329549978

Journal Mail Official journal.biota@gmail.com

Editorial Address Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA