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Brigitta Laksmi Paramita
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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 21 Documents
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Search results for , issue "Vol 12, No 2 (2007): June 2007" : 21 Documents clear
Isoflavon Kedelai Diperkaya dengan Zn sebagai Suplemen Antiarterosklerosis Wanita Premenopause Hery Winarsi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2638

Abstract

This research was conducted to observe the effect of soy isoflavone and Zn on totalcholesterol, LDL, TG and HDL levels of premenopausal women. Thirty-threepremenopausal women in Purwokerto were given supplement containing soy isoflavoneand Zn during 2 months. In the control group (SO), 11 women were given placebo; inthe group SI, 11 women were given the supplement containing soy isoflavone; and in thegroup SIZ, 11 women were given the supplement containing soy isoflavone and Zn.Blood samples were taken 3 times, i.e. baseline, 1 and 2 month after intervention. Oneml of blood was taken by venoject-heparin, intravenously. Blood plasma was used todetermine total cholesterol, LDL, HDL, and TG levels using spectrophotometer. Dataobtained were analysed by ANOVA. The total cholesterol, LDL, and TG plasma levelsdecreased, from 189,27 to 151,63 mg/dl (p=0,02); from 138,97 to 93,93 mg/dl (p=0,004);and from 208,81 to 121,09 mg/dl (p=0,012) respectively. On the contrary HDL levelincreased, from 48,38 to 67,27 mg/dl (p=1,72E-05) after 2 months of intervention. Soyisoflavone and Zn (SIZ) have potential as antiatherosclerosis supplement.
Toksisitas Ekstrak Kasar Bunga dan Daun Ketepeng Cina (Senna alata L. Roxb.) terhadap Larva Udang Artemia salina Leach Hartati Soetjipto; A. Ign. Kristijanto; Rica Susy Asmorowati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2645

Abstract

Extract of Candlebush (Senna alata L. Roxb) flowers and leaves show toxicity effect on Artemia salina Leach. The crude extract was obtained by maceration method using methanol 80% and followed by partition into hexane, chloroform and ethylacetate. Data was analized using Randomized Completely Block Design (RCBD) Sub Sampling, that consisted of six treatments, five replications and three sub samples. To compare the differences between the mortality rater of A. salina, the Honestly Significant Differences (HSD) at 5% level of significance was used. Furthermore, to determine the LC50, the probit analysis was used. The results of this study show that the most effective toxin from all fractions of S. alata was the hexane fraction of the leaves extract. The LC50 of S. alata crude extract of leaves was 187,72 ppm (hexane fraction) and 290,34 ppm (EtOAc fraction). The crude extract of S. alata flowers toward A. salina were 210,77 ppm (hexane fraction) and 354,49 ppm (EtOAc fraction). In general, this study showed that all fractions which were tested had the toxic activity on A. salina.
Resistensi Kacang Tanah Transgenik yang Membawa Gen cp PStV Stabil Sampai Tujuh Generasi Silang-Dalam terhadap Peanut Stripe Virus Dwi Hapsoro; Hajrial Aswidinnoor; Jumanto Jumanto; Rusmilah Suseno; Sudarsono Sudarsono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2662

Abstract

Penyakit bilur kacang tanah yang disebabkan oleh peanut stripe virus (PStV)merupakan salah satu penyakit utama pada pertanaman kacang tanah (Arachishypogaea L.). Penggunaan varietas tahan merupakan alternatif paling efektif untukmengatasi penyakit tersebut. Rekayasa genetika merupakan metode efektif untukmendapatkan varietas kacang tanah yang resisten PStV. Tujuan penelitian ini adalahuntuk (1) mengetahui respons tanaman kacang tanah transgenik yang membawa gen cpPStV terhadap infeksi PStV dan (2) menguji stabilitas transgen sampai tujuh generasisilang-dalam. Tanaman kacang tanah transgenik cv. Gajah generasi T0, T1, T2, T3, T5,T6, dan T7 diinokulasi secara mekanik dengan PStV. Terdapat tiga jenis respons kacangtanah transgenik terhadap infeksi PStV, yaitu resisten, recovery, dan rentan. Padatanaman resisten gejala tidak muncul. Pada tanaman recovery, gejala chlorotic ringmottle muncul pada satu daun atau lebih, selanjutnya gejala tidak tampak pada daundaunyang tumbuh kemudian. Pada tanaman rentan, gejala severe blotch muncul padasuatu daun, selanjutnya gejala tersebut tetap muncul pada seluruh daun yang tumbuhkemudian. Transgen cp PStV tetap stabil setelah mengalami tujuh generasi silangdalam.Sejumlah galur murni kacang tanah transgenik yang resisten PStV telahdiidentifikasi.
Studi Kepiting Mangrove di Delta Mahakam, Kalimantan Timur Rianta Pratiwi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2663

Abstract

The research was carried out in July 2004 at Makaham Delta, East Kalimantan. Thisresearch observed the diversity and the distribution pattern of mangrove crabs atmuara Ilu, Kaely, Bayor, and Beji. There are 33 species consisting of 7 families and 6908individu of crabs found in this research. Most of the mangroves crabs are clumpdistributed. The research method by using frame transects 1 m2 x 1 m2 with 5 up to 10meters distance (based on the thickness of mangrove in those areas). The distributionpattern of crabs analyze by using Index Morisita according to Brower and Zar.
Kehadiran Mamalia pada Sesapan (Salt lick) di Hutan Lindung Taratak, Kabupaten Pesisir Selatan, Sumatera Barat Wilson Novarino; Santi N Kamilah; Agung Nugroho; M. Nazri Janra; M. Silmi; M. Syafrie
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2664

Abstract

The study of mammals visitation on salt licks in protected forest of Taratak, SoutheraCoast of West Sumatra was conducted from June to September 2005 (in total 6840hours.camera). Three camera traps were deployed separately on three salt lick areas.Nine species of mammals were recorded during the study. Tapirus indicus was thespecies most often photographed (13.5%), followed by Presbytis melalophos (11.3%) andSus scrofa (9.8%). This study also records the activity of human on salt licks areas. Thenumbers of visitation of mammals on three salt licks area differed, both in the numberof species and the number of individual. The differences of mammal visitation on saltlicks were not affected by size, altitude, and distances from village. The results alsoshowed that there was time segregation when entering the salt licks area, both interrestrial mammals and primates.
Pengaruh Penggunaan Umpan dan Konstruksi Funnel terhadap Hasil Tangkapan Bubu Laut Dalam di Teluk Palabuhanratu Ari Purbayanto; Adi Susanto; Eddi Husni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2665

Abstract

The main objective of this research was to analyze the influence of bait and funnel constructions on the catch of deep sea pot in Palabuhanratu Bay. The experimental fishing used each of 5 units rigid and soft funnel of the deep sea pots filled with bait of shark and ray meat. The result showed that shark and ray baits did not have a significant influence on the weight of pots catch. The rigid and soft funnel constructions also did not have a significant influence on the catch. Therefore, both shark and ray baits used on the deep sea pots for catching fish at the depth of 200 m in Palabuhanratu Bay had the same effectiveness. The rigid funnel pot was a more suitable construction to catch fish, because it was easy to construct, relatively heavy and fast to sink, so it reduced the setting time.
Alteration of Ecdysteroid Titre by Thyroxine and Juvenile Hormone Analogue (Methoprene) in Bombyx mori (Lepidoptera: Bombycidae) Intan Ahmad; Resti Rahayu; Agus D. Permana; Sita Astari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2666

Abstract

Penelitian ini dilakukan untuk mengetahui pengaruh hormon tiroksin dan analog hormon juvenil (metopren) terhadap pola titer hormon ekdisteroid hemolimf selama instar lima Bombyx mori. Instar dua B. mori diberi pakan daun murbei yang telah diberi tiroksin dengan konsentrasi 1500 ppm. Larva kemudian dibiarkan ganti kulit sampai instar tiga dan empat tanpa perlakuan. Tujuh puluh dua jam setelah ganti kulit pada instar lima, larva disuntik sebanyak 10 μl metopren dengan konsentrasi 15 atau 25 ppm. Titer ekdisteroid hemolimf dideteksi dengan Kromatografi Cair Kinerja Tinggi. Hasil yang diperoleh menunjukkan bahwa perlakuan dengan tiroksin ataupun kombinasi tiroksin-metopren menyebabkan terjadinya perubahan pola titer ekdisteroid dibandingkan dengan kontrol. Ekdisteroid terdeteksi dua hari lebih awal pada perlakuan tiroksin dan titer ekdisteroid pada puncak tertinggi lebih tinggi jika dibandingkan dengan kontrol. Kombinasi tiroksin-metopren tampaknya menyebabkan penekanan terhadap titer ekdisteroid. Hal ini terlihat dengan terjadinya penurunan titer ekdisteroid segera setelah penyuntikan dengan metopren sebelum mencapai titer puncak tertinggi.
Pengaruh Bahan Organik dan Suhu Pengeringan terhadap Ketahanan Hidup Aspergillus niger dalam Pupuk Pelet Bio-fosfat Yudi Sastro; Donny Widianto; Irfan D. Prijambada
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2667

Abstract

This research was aimed to investigate the effect of organic matter and drying temperature on Aspergillus niger survivability in the rock phosphate pellet fertilizer namely bio-phosphate. The research was arranged by a completely randomized design 3x3x6. Addition of the mixing of tapioca waste, rice bran, and starch (BOC) and the humic substance (BH) in the bio-phosphate, and its drying temperature (SP) were the treatments. Aspergillus niger inoculums survivability in the bio-phosphate was determined using plating methods. The result showed that the addition of BOC decreased amount of A. niger in the bio-phosphate up to 28.0%, while the BH increased the amount of A. niger up to 24.4%. The ideal drying temperature of bio-phosphate pellet fertilizer was 600C.
The Growth of Giant Clams Juvenil is Influenced by Nutrient Addition Ambariyanto Ambariyanto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2668

Abstract

Ekosistem karang merupakan daerah yang memiliki produktivitas tinggi namun kandungan nutrient anroganiknya sangat rendah. Sehingga dapat diduga bahwa kenaikan nutrient anorganik dalam ekosistem ini akan mempengaruhi kehidupan organisme yang ada di dalamnya. Penelitian ini bertujuan untuk melihat pengaruh peningkatan nutrien yakni N dan P terhadap pertumbuhan juvenil Kima. Hasil penelitian menunjukkan bahwa dengan penambahan nutrien N secara nyata meningkatkan pertumbuhan juvenil Kima tersebut. Di lain pihak penambahan P tidak berpengaruh terhadap laju pertumbuhan juvenil Kima. Hasil ini menunjukkan bahwa penambahan ammonium seharusnya dilakukan di hatchery untuk meningkatkan pertumbuhan dan produksi Kima.
Keragaman Genetik Bulu Babi (Echinoidea) Abdul Hamid A. Toha
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2669

Abstract

Bulu babi adalah hewan Avertebrata laut yang kaya manfaat. Organisme yang tergolong dalam kelas Echinoidea ini dapat dimanfaatkan sebagai sumber pangan bergizi (Darsono & Sukarno, 1993; Nontji, 2002), berguna dalam ekologi (Lawrence, 1975; Lang & Schroeter, 1976), dan bernilai ekonomis penting. Bulu babi juga berfungsi sebagai organisme hiasan dan digunakan dalam bidang kesehatan untuk pengobatan penyakit (Angka & Suhartono, 2000). Beberapa ahli menggunakan bulu babi sebagai salah satu organisme paling populer untuk mempelajari biologi reproduksi (Vacquier et al., 1995), embriologi (Davidson et al., 1998; Lee et al., 1999), toksikologi (Dinnel et al., 1989), regulasi gen (Davidson et al., 2002), dan biologi evolusi (Peterson et al., 2000).

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