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INDONESIA
Indonesian Journal of Mathematics and Natural Sciences
Published by Universitas Negeri Semarang
ISSN : 02159945     EISSN : 27747832     DOI : https://doi.org/10.15294/ijmns
Core Subject : Education,
Mathematics
The scope of the journal includes the following areas of research: Natural Sciences, Mathematics, and Applied Sciences
Arjuna Subject : Matematika - Matematika (Lain-Lain)
Articles 12 Documents
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Search results for , issue " Vol 39, No 2 (2016): October 2016" : 12 Documents clear
AISOLASI DAN UJI DAYA ANTIMIKROBA EKSTRAK KULIT NANAS (Ananas comosus L. Merr) Setiawan, M H; Mursiti, S; Kusumo, E
Jurnal MIPA Vol 39, No 2 (2016): October 2016
Publisher : Jurnal MIPA

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Abstract

Kandungan senyawa flavonoid dalam limbah kulit nanas memiliki prospek positif sebagai antimikroba. Penelitian ini bertujuan untuk mengetahui efektivitas antimikroba ekstrak kulit nanas basah (KNB) dan ekstrak kulit nanas kering (KNK) dan mengetahui jenis senyawa flavonoid yang berperan sebagai antimikroba terhadap S. aureus dan E. coli. Flavonoid diisolasi menggunakan metode Domestic Microwave Maceration Extraction (DMME) dan partisi. Aktivitas antimikroba diuji menggunakan metode difusi sumuran. Berdasarkan hasil penelitian, ekstrak etil asetat KNB maupun ekstrak etil asetat KNK berpotensi sebagai antimikroba, namun ekstrak etil asetat KNB lebih efektif menghambat bakteri S. aureus dan E. coli dengan diameter daerah hambat (DDH) berturut-turut 13 mm dan 15 mm dibanding ekstrak etil asetat KNK dengan nilai DDH berturut-turut 12 dan 14,25 mm. Senyawa flavonoid dalam kulit nanas yang berperan sebagai antimikroba diduga merupakan golongan flavanon (KNB) dan dihidroflavonol (KNK).Flavonoid compound in pineapple peel waste have positive prospects for antimicrobial. This study aims at understanding effectiveness of wet pineapple peel extract and dried pineapple peel extract antimicrobial and to know the type of flavonoid compound which act as antimicrobial against S.aureus and E.coli. Flavonoid was isolated using Domestic Microwave Maceration Extraction (DMME) and partitions. Antimicrobial activity was tested using pitting diffusion method. Based on the research result, wet pineapple peel extract and dried pineapple peel extract are potential to be antimicrobial. However wet pineapple peel extract is more effective to inhibit bacteria S.aureus and E.coli with Inhibitory Regional Diameter respectively 13 mm and 15 mm compared to dried pineapple peel extract with Inhibitory Regional Diameter respectively 12 and 14,25 mm. Flavonoid compound in pineapple peel that acts as an antimicrobial is thought to be flavanones (wet pineapple peel) and dihydroflavonol (dried pineapple peel).
PERKEMBANGAN OVARIUM TIKUS YANG DIPAPAR RADIASI SINAR X Nurjanah, N; Widiyaningrum, P
Jurnal MIPA Vol 39, No 2 (2016): October 2016
Publisher : Jurnal MIPA

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Abstract

Penelitian ini bertujuan untuk mengetahui perkembangan ovarium tikus putih (Rattus norvegicus) galur Wistar yang dipapar radiasi sinar X, meliputi bobot dan gambaran histologisnya.  Penelitian menggunakan 20 ekor tikus betina usia 1,5 bulan dengan bobot badan rata-rata 150±13 gram.  Tikus  dikelompokkan ke dalam 4 perlakuan yaitu  perlakuan dosis radiasi sinar X sebesar 50, 100 dan 150 mGray serta satu kelompok kontrol. Variabel yang diukur meliputi bobot ovarium utuh dan gambaran histologis ovarium. Radiasi dilakukan dengan cara penyinaran satu kali tembakan pada setiap tikus per hari selama 5 hari.  Analisis data bobot ovarium dilakukan dengan dengan anova satu arah dilanjutkan dengan uji LSD pada taraf signifikansi 5%, sedangkan untuk data kualitatif gambaran histologis ovarium dianalisis secara deskriptif. Hasil analisis statistik memperlihatkan bahwa perlakuan dosis radiasi 50, 100 dan 150 mGray berpengaruh nyata terhadap bobot ovarium, yakni cenderung menurun dibanding kelompok kontrol. Pengamatan terhadap gambaran histologis ovarium memperlihatkan kerusakan ovarium mulai terjadi pada dosis 100 dan 150 mGray. Penelitian ini menyimpulkan bahwa paparan radiasi sinar X menyebabkan penurunan bobot ovarium dan pada dosis 100 mGray telah menimbulkan tanda kerusakan pada struktur histologi ovarium tikus putih galur Wistar.This study aims to determine ovarian development of rats (Rattus norvegicus) after exposure X-ray radiation, especially weights and ovarian histology. The study used 12 female rats of 1.5 months and average body weight of 150 ± 13 grams. Female rats were devided into four groups: control, and variations in dose of 50 mGray (T1), 100 mGray (T2) and 150 mGray (T3). This study used a complete randomized block design.  Radiation is done by irradiating one shot on each rat per day for 5 days. Statistical analysis for ovarian weight was performed by one way Anova and LSD Test, while qualitative data of ovarian histological were analyzed descriptively. The results showed that the variations in dose of  X-ray radiation significantly (P ≤ 5 %) of the ovarian weight. LSD test results further demonstrate that treatment of radiation doses 50, 100 and 150 mGray significant effect on ovarian weight, which decreases compared to the control group. This study concluded that exposure to X-ray radiation causes a decrease in ovarian weight and radiation dose of 100 mGray has caused damage to the structure of the ovarian histology Wistar rats.

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