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INDONESIA
Medical Journal of Indonesia
Published by Universitas Indonesia
ISSN : 08531773     EISSN : 22528083     DOI : 10.13181
Core Subject : Health,
Medicine & Pharmacology
This quarterly medical journal is an official scientific journal of the Faculty of Medicine Universitas Indonesia in collaboration with German-Indonesian Medical Association (DIGM) Indexed in: IMSEAR; CAB Abstracts; Global Health; HINARI; DOAJ; DRJI; Google Scholar; JournalTOCs; Ulrichsweb Global Serial Directory; WorldCat; New Jour; Electronic Journals Library; ISJD Accredited (2013-2018) by DIKTI Kemendikbud Republik Indonesia (No:58/DIKTI/Kep/2013)
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Articles 11 Documents
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Search results for , issue "Vol. 17 No. 3 (2008): July-September" : 11 Documents clear
Significance of ocular stem cells in tissue engineering of the eye
Medical Journal of Indonesia Vol. 17 No. 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.v17i3.312

Abstract

Blindness is one of the most devastating condition for mankind. Regenerative medicine through tissue engineering and optimizing tissue regeneration through local adult stem cell differentiation or stem cell transplantation has received a lot of attention. This review presents highlights in the discovery of ocular stem cell and in vivo experiment for stem cell transplantation and current trends in tissue engineering of the eye. (Med J Indones 2008; 17: 143-8)
Testosterone undecanoate and depo medroxyprogesterone acetate induced azoospermia through increased expression of spermatogenic cell caspase 3
Medical Journal of Indonesia Vol. 17 No. 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.v17i3.313

Abstract

The administration of a combination of testosterone undecanoate (TU, a long-acting androgen) and depo-medroxyprogesterone acetate (DMPA) were investigated in term of suppression of rat sperm concentration in vivo to azoospermia through increasing activity of spermatogenic cell caspase 3. Adult Sprague Dawley rats received TU and DMPA of 2.5 mg and 1.25 mg, respectively, a regimen known to rapidly reduce intra testicular testosterone and to produce azoospermia within 12 weeks. Caspase 3 positive sperm cells increased compared with control levels during 6 weeks post-injection and increased further through 60 weeks. Immunohistochemistry for caspase 3 revealed that spermatocytes represented the predominant caspase 3 positive germ cells. Modest immunoreactivity for caspase-3 was localized to nuclear region of the germ cells of control and treated testes. Immunohistochemistry study revealed significantly increased caspase-3 expression in nuclei of germ cells during administration of TU+DMPA to rats. Additionally, the caspase 3 content was significantly increased in germ cells during rats were administered TU+DMPA (453.90±84.88 cells/200 seminiferous tubules) and caspase 3 significant increase in immunoreactivity was localized to the nuclei of spermatogonia, spermatocytes, and spermatids. Taken together, these results indicated that azoospermia due to reduced intratesticular testosterone concentration was caspase-3 activation dependent and suggested that the increase in active caspase-3 in the nucleus may be involved in the induction of decreased sperm production. (Med J Indones 2008; 17: 149-56)
The extract of "shoe flower" (Hibiscus rosea sinensis L) leaves inhibit the spermatogenesis of ddy strain mice
Medical Journal of Indonesia Vol. 17 No. 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.v17i3.314

Abstract

This study was conducted in order to develop male contraception from plants, namely the "shoe flower" (Hibiscus rosea sinensis L) leaves. The objective of this study was to find out whether the extract of "shoe flower" leaves could inhibit the process of spermatogenesis on ddy strain mice. This research was performed in 3 groups and each group consisted of 8 mice. The control group was given 1% carboxy methyl cellulose (CMC) in 0.5 ml aquabides. The treatment group I was given the extract of "shoe flower" leaves 700 mg/kg BW and 1% CMC in 0.5 ml aquabides, and the second treatment group was given the extract of "shoe flower" leaves, 800 mg/kgBW and 1% CMC in 0.5 ml aquabides. The treatment were given for 40 days in accordance with the spermatogenesis cycle. Then, the production of histological slides of the mice testis and the observation of the slides using light microscope with magnification of 100x and 400x were done. Further, counting of the spermatogenic cells was done. At last the pictures of seminiferous tubulus cross-section of the three groups which consisted of spermatogenic cells were taken through light microscope with magnification of 100x and 400x using Fuji camera and Fuji film, 200 ASA. The results showed significant differences between the control, treatment I, and treament II group. There were decreased numbers of spermatogonia, pachyten primary spermatocytes and spermatids in treatment groups (P<0.01). The result of this study showed that the extract of "shoe flower" (Hibiscus rosea sinensis L) leaves, inhibited the process of spermatogenesis of ddy strain mice. It is hoped that the result of this study can be developed into a male contraception. (Med J Indones 2008; 17: 157-62)
Autopsy findings in severe malaria - a case report
Medical Journal of Indonesia Vol. 17 No. 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.v17i3.315

Abstract

Severe malaria, caused by Plasmodium falciparum infection, has a high mortality rate and is the main cause of death in malaria. Since clinical autopsy is unpopular in Indonesia, autopsy examination in malaria cases is rarely done. We reported a forty three year old woman from non endemic area that was dead because of severe malaria. Diagnosis was concluded from autopsy, histopathology, and toxicology. (Med J Indones 2008; 17: 210-5)
Non functional adrenocortical carcinoma
Medical Journal of Indonesia Vol. 17 No. 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.v17i3.316

Abstract

Adrenocortical carcinoma is a rare disease with an incidence of approximately 1-2 per million populations per year. The main symptoms are abdominal mass with or without endocrine dysfunction. However, there are difficulties in the diagnosis and treatment of this case due to its unspecific clinical presentation which sometimes require additional diagnostic methods such as immunohistochemistry. The prognosis is often poor, especially when it's impossible to resect the entire tumor. We present one case of adrenocortical carcinoma in RSCM which was diagnosed through immunohistochemistry examination postoperatively in patient with giant intra-abdominal tumor (mesenteric cyst). (Med J Indones 2008; 17: 203-9)
Asthma symptoms improvement in moderate persistent asthma patients with gastroesophageal reflux disease (GERD): the role of proton-pump inhibitor
Medical Journal of Indonesia Vol. 17 No. 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.v17i3.317

Abstract

This study aimed to evaluate effect of proton pump inhibitor (esomeprazole) on asthma symptoms, use of inhaled bronchodilator and peak expiratory flow rate (PEFR) in moderate persistent asthma with gastroesofageal refluks disease (GERD). This randomized single blind, controlled clinical trial study was conducted at Persahabatan Hospital, Jakarta from July 2004 until October 2005. Samples were moderate persistent asthma patients with GERD. GERD is diagnosed GERD symptoms and proof of oesophagitis from endoscopy and or histapatologic examination from oesophagus biopsy. Phase 1:2 week run-in period patient received inhaled budesonide 2x200 ug/day. Phase 2: patient randomised to receive inhaled budesonide 2 x 400 ug/day with esomeprazole 40 mg/day or without esomeprazole (control group) for 8 weeks. Phase 3: 4 week wash out period, patient receive inhaled budesonide 2 x 200 ug/day. Diary cards were assessed at run-in periode, after treatment 4 weeks, 8 weeks and wash out. There were 32 patients (23 female and 9 male) completed the study. Mean total asthma symptoms score daily were significantly decreased on esomeprazole vs without esomeprazole after 8 weeks (-2.29 vs -0.90; p < 0.05). Mean use of inhaled bronchodilator was significantly decreased on esomeprazole vs without esomeprazole after 8 weeks (-1.09 vs -0.42; p < 0.05). Morning and evening PEFR improved higher on esomeprazole than without esomeprazol but were not significantly difference. In conclusion, administration esomeprazole 40 mg daily improved asthma symptoms and lower the use of inhaled bronchodilator in moderate persistent asthma patients with GERD. (Med J Indones 2008; 17: 169-74)
Surgical management of benign peripheral nerve tumors
Medical Journal of Indonesia Vol. 17 No. 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.v17i3.318

Abstract

Peripheral nerve tumors are rare lesions that can arise anywhere in the body and hence have a myriad of wide differential diagnosis. They commonly present as a non-specific mass which is diagnosed as a peripheral nerve tumor at surgery. While these tumors may initially be referred to a wide variety of surgeons, early recognition of the nature of the lesion and appropriate surgical treatment by an expert peripheral nerve surgeon is essential in order to minimize post-operative neurological deficits. The objective of this article is to provide a general management scheme for the most common setting of benign peripheral nerve tumors. (Med J Indones 2008; 17: 163-8)
Cluster analysis of typhoid cases in Kota Bharu, Kelantan, Malaysia
Medical Journal of Indonesia Vol. 17 No. 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.v17i3.319

Abstract

Typhoid fever is still a major public health problem globally as well as in Malaysia. This study was done to identify the spatial epidemiology of typhoid fever in the Kota Bharu District of Malaysia as a first step to developing more advanced analysis of the whole country. The main characteristic of the epidemiological pattern that interested us was whether typhoid cases occurred in clusters or whether they were evenly distributed throughout the area. We also wanted to know at what spatial distances they were clustered. All confirmed typhoid cases that were reported to the Kota Bharu District Health Department from the year 2001 to June of 2005 were taken as the samples. From the home address of the cases, the location of the house was traced and a coordinate was taken using handheld GPS devices. Spatial statistical analysis was done to determine the distribution of typhoid cases, whether clustered, random or dispersed. The spatial statistical analysis was done using CrimeStat III software to determine whether typhoid cases occur in clusters, and later on to determine at what distances it clustered. From 736 cases involved in the study there was significant clustering for cases occurring in the years 2001, 2002, 2003 and 2005. There was no significant clustering in year 2004. Typhoid clustering also occurred strongly for distances up to 6 km. This study shows that typhoid cases occur in clusters, and this method could be applicable to describe spatial epidemiology for a specific area. (Med J Indones 2008; 17: 175-82)
Decompression sickness among Moroami diving fishermen in Jakarta
Medical Journal of Indonesia Vol. 17 No. 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.v17i3.320

Abstract

Indonesia is an archipelago with many traditional divers, however research on decompression sickness (DCS) has not yet elaborated. The aim of the study was to identify the prevalence of DCS and factors related to it. The study was conducted on October-November 2007 among fisherman moroami divers in Seribu Island Jakarta. Anamnesis and physical examination was taken before and three times after diving. Subject was diagnosed as having DCS if experienced one of these symptom or sign: myalgia, muscle pain, skin rash, ankle weakness, bowel movement & bladder dysfunction, visual disturbances, headache, vertigo, dyspnoe, chest pain, convulsion, unconsciousness, nausea and vomiting. Among 123 potential divers, five were having upper respiratory infection, so only 117 divers participated in this study. Final model analysis showed that regulator, valsava when having ear pain, ascending speed to surface, and lack of training were risk factors to obtain DCS. Divers whose ascending speed more than 9 m per minutes had two times risk to get DCS [adjusted ratio = 2.2; 95% confidence interval (CI)= 1.11 - 3.56]. Having DCS before diving, increased risk 20% (RRa = 1.20; 95% CI = 0.86-1.68; P=0,285). Beside knowledge to use regulator correctly and valsava, fisherman Moroami divers need to be trained to ascend speed to sea level surface less than 9 m per minute. (Med J Indones 2008; 17: 197-202)
Detection of Mycobacterium avium subspecies paratuberculosis in formula milk from Bogor using PCR IS 900
Medical Journal of Indonesia Vol. 17 No. 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.v17i3.321

Abstract

Crohn's disease (CD) that becomes a public health concern in developed countries shows similarities in clinical signs and pathological features with Johne's disease (JD) in ruminants infected by Mycobacterium avium subspecies paratuberculosis (MAP). Few researches conducted in Europe, the USA, and Australia showed relationships between MAP, CD, JD and dairy products. Indonesians consume milk and diary products from domestic and imported source. Adji in 2004 found some domestic dairy cows that were seropositive for MAP, and this could be a serious problem in dairy farm animals and human health in the future. The aim of this study was to detect MAP in the growing up formula milk. Fifty samples from five established factories were taken from supermarkets in Bogor. Polymerase chain reaction method (PCR) with insertion sequence (IS) 900 as primer and culture in Herrold's egg yolk media with mycobactin J (HEYM J) as a gold standard were used in this study. Neither MAP grew up in HEYM J medium after 20 weeks of culture period nor positive samples by PCR IS 900 were found. Although there were no positive samples found in this study, further extensive and comprehensive studies on MAP should be done with more and varied samples, as well as in human to provide data on MAP in Indonesia. (Med J Indones 2008; 17: 183-7)

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