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INDONESIA
Indonesian Journal of Agricultural Science
Published by Kementerian Pertanian
ISSN : 1411982X     EISSN : 23548509     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The journal publishes primary research articles from any source if they make a significant original contribution to the experimental or theoretical understanding of some aspect of agricultural science in Indonesia. The definition of agricultural science is kept as wide as possible to allow the broadest coverage in the journal. Accredited base on Director Decree of Indonesian Institute of Science No. 816/D/2009 with acreditation No. 172/AU1/P2MBI/08/2009. IJAS is newly accredited by the Indonesian Institute of Science in 2012 (No. 473/AU2/P2MI-LIPI/08/2012) for period of 2012-2015.
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 17, No 1 (2016): April 2016" : 5 Documents clear
EFFECT OF DIFFERENT SOURCES OF PLANT GROWTH REGULATOR ON THE INDUCTION AND DEVELOPMENT OF MANGOSTEEN SOMATIC EMBRYOS Yosi Zendra Joni; Riry Prihatini; Darda Efendi; Ika Roostika
Indonesian Journal of Agricultural Science Vol 17, No 1 (2016): April 2016
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v17n1.2016.p9-16

Abstract

Somatic embryogenesis is a technique for regenerating embryos derived from somatic cells of various plant species. This technique along with the utilization of plant growth regulator (PGR) might benefit for mass propagation and improvement of plant species through biotechnological tools. The study aimed to determine the effect of different plant growth regu-lators, namely 6-benzyladenine (BA) and thidiazuron (TDZ) on the embryogenic callus induction as well as casein hydrolysate and malt extract on the somatic embryo development of mangosteen. The explants used were in vitro young stems of mangosteen clone Leuwiliang. This study consisted of two experiments, namely induction of embryogenic callus and formation of somatic embryo. The first experiment was arranged as factorial in a completely randomized design with BA (0 and 0.7 mg l-1) as the first factor and TDZ (0, 0.1, 0.5 and 1.0 mg l-1) as the second factor. The second experiment consisted of four treatments, i.e. casein hydrolysate and malt extract at the rate of 500 and 1,000 mg l-1. The results showed that the best medium for embryogenic callus induction was MS supplemented with 0.1 mg l-1 TDZ, which resulted semifriable calli. Casein hydrolysate and malt extract could not induce the formation of somatic embryos. After two times subcultures on the same MS medium supplemented with 0.5 mg l-1 TDZ and 0.7 mg l-1 BA, a total of 33.8 somatic embryos per explant was induced. The successful somatic embryogenesis would support mangosteen breeding and in vitro mass propagation program.
DEVELOPMENT OF EST-SSR MARKERS TO ASSESS GENETIC DIVERSITY OF BROCCOLI AND ITS RELATED SPECIES Nur Kholilatul Izzah; Reflinur Reflinur; Tae-Jin Yang
Indonesian Journal of Agricultural Science Vol 17, No 1 (2016): April 2016
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v17n1.2016.p17-26

Abstract

Development of Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) markers derived from public database is known to be more efficient, faster and low cost. The objective of this study was to generate a new set of EST-SSR markers for broccoli and its related species and their usefulness for assessing their genetic diversity. A total of 202 Brassica oleracea ESTs were retrieved from NCBI and then assembled into 172 unigenes by means of CAP3 program. Identification of SSRs was carried out using web-based tool, RepeatMasker software. Afterwards, EST-SSR markers were developed using Primer3 program. Among the identified SSRs, trinucleotide repeats were the most common repeat types, which accounted for about 50%. A total of eight primer pairs were successfully designed and yielded amplification products. Among them, five markers were polymorphic and displayed a total of 30 alleles with an average number of six alleles per locus. The polymorphic markers were subsequently used for analyzing genetic diversity of 36 B. oleracea cultivars including 22 broccoli, five cauliflower and nine kohlrabi cultivars based on genetic similarity matrix as implemented in NTSYS program. At similarity coefficient of 61%, a UPGMA clustering dendrogram effectively separated 36 genotypes into three main groups, where 30 out of 36 genotypes were clearly discriminated. The result obtained in the present study would help breeders in selecting parental lines for crossing. Moreover, the novel EST-SSR markers developed in the study could be a valuable tool for differentiating cultivars of broccoli and related species.
SENSITIVITY OF PIGMENT CONTENT OF BANANA AND ORCHID TISSUE CULTURE EXPOSED TO EXTREMELY LOW FREQUENCY ELECTROMAGNETIC FIEL Riry Prihatini; Norihan Mohamad Saleh
Indonesian Journal of Agricultural Science Vol 17, No 1 (2016): April 2016
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v17n1.2016.p37-34

Abstract

Natural exposure of extremely low frequency electromagnetic field (ELF-EMF) occurs in the environment and acts as one of the abiotic factors that affect the growth and development of organisms. This study was conducted to determine the effect of ELF-EMF on the tissue cultured banana and slipper orchid chlorophyll content as one of the indicators in measuring plant photosynthetic capacity. Four days old banana (Musa sp. cv. Berangan) corm and seven days old slipper orchid (Paphiopedilum rothschildianum) cultures were exposed to 6 and 12 mT ELF-EMF generated by controllable ELF-EMF built up machine for 0.5, 1, 2 and 4 hours. After exposure, the banana and orchid cultures were incubated at 25° C for 8 and 16 weeks, respectively. The results showed that the ELF-EMF exposure had different effects on banana and slipper orchid cultures though both plant species belong to monocotyledon. The highest increase in chlorophyll content on banana was resulted by the high intensity and long duration of ELF-EMF exposure (12 mT for 4 hours), whereas on slipper orchid the modest and short duration of ELF-EMF exposure produced the most excessive chlorophyll content. Different ELF-EMF exposures (12 mT for 4 hours and 6 mT for 30 minutes) had potential to be applied on each plant to improve in vitro plant (banana and slipper orchid, respectively) growth. The increased chlorophyll and carotene/xanthophyll content on banana indicated that the banana was more tolerant to ELF-EMF exposure compared to slipper orchid. 
EFFECT OF GAMMA IRRADIATION ON THE GROWTH AND DEVELOPMENT OF SAGO PALM (Metroxylon sagu Rottb.) CALLI Imron Riyadi; Sumaryono Sumaryono
Indonesian Journal of Agricultural Science Vol 17, No 1 (2016): April 2016
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v17n1.2016.p35-40

Abstract

The application of gamma irradiation on plant materials may increase the genetic variation of the offspring with useful traits. The experiment was conducted to determine the effect of irradiation dosage of gamma ray on growth and development of sago palm (Metroxylon sagu) calli. Friable calli of sago palm derived from suspension culture were used as a material source. The primary calli were initiated from apical meristematic tissues of sago palm suckers of Alitir variety from Merauke, Papua. The treatments used were dosage of gamma ray irradiation at 0, 5, 10, 15, 20 and 25 Gy. The treated calli were then subcultured on modified Murashige and Skoog (MMS) solid medium containing 3% sucrose and 0.1% activated charcoal and added with 1 mg l-1 2,4-D and 0.1 mg l-1 kinetin. The results showed that at all irradiation dosages, calli biomass increased significantly. The highest proliferation of calli biomass of 5.33 folds from the initial culture after 4 weeks was achieved at gamma irradiation of 25 Gy, whereas the lowest proliferation of calli biomass of 3.4 folds was achieved at control. The best development of embryogenic calli was obtained at 10 Gy that produced 100% somatic embryos, whereas the lowest somatic embryo formation at 0% was obtained at 0 and 25 Gy after one subculture. High response of somatic embryo induction to gamma irradiation at 10 Gy may increase production of somatic embryos. These results can be used in in vitro breeding of sago palm via mutagenesis to create new elite varieties.
IRON DYNAMICS AND ITS RELATION TO SOIL REDOX POTENTIAL AND PLANT GROWTH IN ACID SULPHATE SOIL OF SOUTH KALIMANTAN, INDONESIA Wahida Annisa; Dedy Nursyamsi
Indonesian Journal of Agricultural Science Vol 17, No 1 (2016): April 2016
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v17n1.2016.p1-8

Abstract

Organic matter has a function to maintain reductive conditions and to chelate toxic elements in acid sulphate soils. The study aimed to assess the dynamics of ferrous iron (Fe2+) in acid sulphate soil and its correlation with soil redox potential (Eh) and plant growth. The experiment was arranged in two factorial randomized block design with three replications. The first factor was two types of organic matter: (1) control (without organic matter), (2) rice straw and (3) rush weed (Eleocharis dulcis). The second factor was time of decomposition of organic matter: I1 = 2 weeks, I2 = 4 weeks, I3 = 8 weeks, and I4 = 12 weeks (farmer practice). The results showed that concentration of ferrous iron in the soil ranged from 782 to 1308 mg kg-1 during the rice growing season. The highest constant rate of iron reduction (k F2+) was observed on application of rice straw and rush weed with decomposition time of 8 weeks with the k Fe2+ value of 0.016 and 0.011 per day, respectively, while the ferrous iron formation without organic matter had the k Fe2+ value of 0.077 per day. The ferric iron (Fe3+) reduction served as a function of soil Eh as indicated by the negative correlation of ferrous iron and Eh (r = -0.856*). Organic matter decreased exchangeable iron due to chelating reaction. Iron concentration in roots was negatively correlated with soil soluble iron (r = -0.62*). Application of rice straw decomposed for 8 weeks increased the height of rice plant up to 105.67 cm. The score of Fe2+ toxicity at 8 weeks after planting ranged from 2 to 3, so rice crop did not show iron toxicity symptoms. 

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