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INDONESIA
Indonesian Journal of Agricultural Science
Published by Kementerian Pertanian
ISSN : 1411982X     EISSN : 23548509     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The journal publishes primary research articles from any source if they make a significant original contribution to the experimental or theoretical understanding of some aspect of agricultural science in Indonesia. The definition of agricultural science is kept as wide as possible to allow the broadest coverage in the journal. Accredited base on Director Decree of Indonesian Institute of Science No. 816/D/2009 with acreditation No. 172/AU1/P2MBI/08/2009. IJAS is newly accredited by the Indonesian Institute of Science in 2012 (No. 473/AU2/P2MI-LIPI/08/2012) for period of 2012-2015.
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Articles 8 Documents
 1 
Search results for , issue "Vol 4, No 1 (2003): April 2003" : 8 Documents clear
SOIL PROPERTIES OF THE EASTERN TOPOSEQUENCE OF MOUNT KELIMUTU, FLORES ISLAND, EAST NUSA TENGGARA AND THEIR POTENTIAL FOR AGRICULTURAL USE ., Hikmatullah; Subagyo, H.; Prasetyo, B.H.
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Kelimutu stratovolcano is one of the young volcanoes extensively found in Flores island. Studies on volcanic soil properties and their suitability for agricultural development in the whole island are lacking. The research was conducted to evaluate the relationship of elevation and soil properties,  and suitability for agricultural development. Five representative pedons at elevation of 550, 1,000, 1,200, 1,400, and 1,600 m above sea level (asl), respectively, were studied in the field, and 22 soil samples were analyzed in the laboratory. The results indicated that elevation significantly affected soil properties and degree of soil weathering. With decreasing elevation, sand content, amorphous material content, and phosphate retention decreased. Concomitantly, clay content, H2O-pH, exchangeable Ca and Mg, base saturation, and soil-CEC increased. A highly positive correlation was shown between P retention and NaFpH, Al, and (Al+0.5 Fe) contents extracted by acid ammonium oxalate. Soil-CEC also showed significant positive correlation with clay and silt contents, organic carbon, and exchangeable Ca and Mg. Degree of weathering increased with decreasingelevation as reflected by decreasing silt/clay ratio. Soils developed from Entisols (Lithic Udorthents) at 1,600 m asl, to Andisols (Typic Hapludands) at 1,000-1,400 m asl, and Mollisols (Typic Hapludolls) at lower elevation down to 550 m asl. Soils at 1,400-1,600 m asl are unsuitable for  agriculture. Soils at 1,000-1,200 m are moderately suitable for food crops,horticulture, and estate crops. Dominant limiting factors are steep slopes and P retention. Soils at 550 m asl are suitable for food and estate crops, and also lowland horticulture.
DETERMINATION OF FOLATE RETENTION DURING TOFU PREPARATION USING TRIENZYME TREATMENT AND MICROBIOLOGICAL ASSAY Ginting, Erliana; Arcot, Jayashree; Chox, Julian M.
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Megaloblastic anaemia, neural tube defects, coronary heart disease, and some forms of cancer have been linked to an inadequate dietary intake of folate. Legumes are good sources of folate, however, processing may affect folate availability in legume foods. This study investigated the folate content and retention during the preparation of tofu through the trienzyme treatment (protease, α-amylase, and human plasma) and microbiological assay using Lactobacillus casei. The results showed that the total folate content was considerably reduced during tofu preparation, from 273.4 µg 100 g-1 dwb in soybean seed to 108.4 µg 100 g-1 dwb in tofu, yielding a folate retentionof 40%. Folate losses were mainly due to leaching into the water during soaking and pressing as well as heat destruction during milling and boiling. High folate loss (51%) was particularly found in the whey. A shorter soaking time (8 hours), optimum extraction, and pressing methods were suggested with respect to minimizing folate losses during tofu preparation. This studysuggests that the consumption of 100 g of deep-fried tofu may satisfy 21% of the recommended dietary intake (RDI).
PRODUCTION OF POLYCLONAL ANTIBODY TO THE COAT PROTEIN OF CITRUS TRISTEZA VIRUS IN CHICKEN EGGS ., Nurhadi; Sijam, Kamaruzaman; Sulaiman, Inon
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Citrus tristeza virus (CTV) is one of the most destructive diseases in many citrus growing areas of Indonesia. Effective strategies for controlling CTV depend on diagnostic procedure namely enzyme-linked immunosorbent assay (ELISA). Study aimed to purify the CTV antigen and produced its polyclonal antibody. Virion of the severe CTV isolate designated UPM/T-002 was concentrated by polyethylene glycol (PEG) precipitation combined with low speed centrifugation. Semipurified antigen was further purified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE). The specific coat protein (CP) band of CTV with molecular weight of 25 kD was excised and eluted using elution buffer containing 0.25 M Tris-HCl pH 6.8 + 0.1% SDS, then used as antigen for injectioninto 6-month-old female of White Leghorn chicken. Results, showed than the specific polyclonal antibody raised against the 25-kDa CP had a titer of approximately 104, gave low background reaction with healthy plant sap and reacted specifically with CTV isolates. The reaction was equally strong for a severe, a moderate, a mild, and a symptomless isolate, suggesting a broad reaction range of this antibody toward different CTV isolates. Optimal virus titer can be obtained since virus loss during purification could be minimized and the highly purified antigen as an immunogen could be obtained by cutting out the CP band from SDS-PAGE gels. Large amount of highly titer of CTV antibody can be produced in chicken egg. The simplicityof the procedure makes it economically acceptable and technically adoptable because the antibody can be produced in basic laboratory.
SCREENING OF SOIL BACTERIA FOR PLANT GROWTH PROMOTION ACTIVITIES IN VITRO Husen, Edi
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Fourteen isolates of soil bacteria, including two known plant growth promoting rhizobacteria (PGPR) strains, Azotobacter vinelandii Mac 259 and Bacillus cereus UW 85, were tested in vitro. Parameters assessed were indoleacetic acid (IAA) production, phosphate solubilization, dinitrogen fixation, and siderophore (Fe-III chelating agent) production. IAA production was assayed colorimetrically using ferric chlorideperchloricacid reagent. Phosphate-solubilization and siderophore production were tested qualitatively by plating the bacteria in Pikovskaya and chrome azurol S agar, respectively. The ability to fix dinitrogen was measured based on nitrogenase activity of the bacteria by gas chromatography. The results showed that twelve isolates produced IAA, ranged from 2.09 to 33.28 µmol ml-1. The ability to solubilize precipitated phosphate waspositively exhibited by four isolates (BS 58, BTS, TCaR 61, and BTCaRe 65). Seven isolates including Mac 259 positively produced siderophore. None of the isolates showed nitrogenase activity. Only one isolate (TS 3) did not exhibit any of the traits tested. Isolate TCeRe 60 and reference strain Mac 259 were found to have IAA- and siderophore-producing traits. Four P-solubilizing bacteria (BS 58, BTS, TCaR 61, and BTCaRe 65)were also IAA- and siderophore-producing bacteria. Potential use of these PGPR isolates needs further test in enhancing plant growth.
SCREENING OF SOIL BACTERIA FOR PLANT GROWTH PROMOTION ACTIVITIES IN VITRO Edi Husen
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v4n1.2003.p27-31

Abstract

Fourteen isolates of soil bacteria, including two known plant growth promoting rhizobacteria (PGPR) strains, Azotobacter vinelandii Mac 259 and Bacillus cereus UW 85, were tested in vitro. Parameters assessed were indoleacetic acid (IAA) production, phosphate solubilization, dinitrogen fixation, and siderophore (Fe-III chelating agent) production. IAA production was assayed colorimetrically using ferric chlorideperchloric acid reagent. Phosphate-solubilization and siderophore production were tested qualitatively by plating the bacteria in Pikovskaya and chrome azurol S agar, respectively. The ability to fix dinitrogen was measured based on nitrogenase activity of the bacteria by gas chromatography. The results showed that twelve isolates produced IAA, ranged from 2.09 to 33.28 µmol ml-1. The ability to solubilize precipitated phosphate was positively exhibited by four isolates (BS 58, BTS, TCaR 61, and BTCaRe 65). Seven isolates including Mac 259 positively produced siderophore. None of the isolates showed nitrogenase activity. Only one isolate (TS 3) did not exhibit any of the traits tested. Isolate TCeRe 60 and reference strain Mac 259 were found to have IAA- and siderophore-producing traits. Four P-solubilizing bacteria (BS 58, BTS, TCaR 61, and BTCaRe 65) were also IAA- and siderophore-producing bacteria. Potential use of these PGPR isolates needs further test in enhancing plant growth.
SOIL PROPERTIES OF THE EASTERN TOPOSEQUENCE OF MOUNT KELIMUTU, FLORES ISLAND, EAST NUSA TENGGARA AND THEIR POTENTIAL FOR AGRICULTURAL USE Hikmatullah Hikmatullah; H. Subagyo; B.H. Prasetyo
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v4n1.2003.p1-11

Abstract

Kelimutu stratovolcano is one of the young volcanoes extensively found in Flores island. Studies on volcanic soil properties and their suitability for agricultural development in the whole island are lacking. The research was conducted to evaluate the relationship of elevation and soil properties,  and suitability for agricultural development. Five representative pedons at elevation of 550, 1,000, 1,200, 1,400, and 1,600 m above sea level (asl), respectively, were studied in the field, and 22 soil samples were analyzed in the laboratory. The results indicated that elevation significantly affected soil properties and degree of soil weathering. With decreasing elevation, sand content, amorphous material content, and phosphate retention decreased. Concomitantly, clay content, H2O-pH, exchangeable Ca and Mg, base saturation, and soil-CEC increased. A highly positive correlation was shown between P retention and NaFpH, Al, and (Al+0.5 Fe) contents extracted by acid ammonium oxalate. Soil-CEC also showed significant positive correlation with clay and silt contents, organic carbon, and exchangeable Ca and Mg. Degree of weathering increased with decreasing elevation as reflected by decreasing silt/clay ratio. Soils developed from Entisols (Lithic Udorthents) at 1,600 m asl, to Andisols (Typic Hapludands) at 1,000-1,400 m asl, and Mollisols (Typic Hapludolls) at lower elevation down to 550 m asl. Soils at 1,400-1,600 m asl are unsuitable for  agriculture. Soils at 1,000-1,200 m are moderately suitable for food crops, horticulture, and estate crops. Dominant limiting factors are steep slopes and P retention. Soils at 550 m asl are suitable for food and estate crops, and also lowland horticulture.
DETERMINATION OF FOLATE RETENTION DURING TOFU PREPARATION USING TRIENZYME TREATMENT AND MICROBIOLOGICAL ASSAY Erliana Ginting; Jayashree Arcot; Julian M. Chox
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v4n1.2003.p12-17

Abstract

Megaloblastic anaemia, neural tube defects, coronary heart disease, and some forms of cancer have been linked to an inadequate dietary intake of folate. Legumes are good sources of folate, however, processing may affect folate availability in legume foods. This study investigated the folate content and retention during the preparation of tofu through the trienzyme treatment (protease, α-amylase, and human plasma) and microbiological assay using Lactobacillus casei. The results showed that the total folate content was considerably reduced during tofu preparation, from 273.4 µg 100 g-1 dwb in soybean seed to 108.4 µg 100 g-1 dwb in tofu, yielding a folate retention of 40%. Folate losses were mainly due to leaching into the water during soaking and pressing as well as heat destruction during milling and boiling. High folate loss (51%) was particularly found in the whey. A shorter soaking time (8 hours), optimum extraction, and pressing methods were suggested with respect to minimizing folate losses during tofu preparation. This study suggests that the consumption of 100 g of deep-fried tofu may satisfy 21% of the recommended dietary intake (RDI).
PRODUCTION OF POLYCLONAL ANTIBODY TO THE COAT PROTEIN OF CITRUS TRISTEZA VIRUS IN CHICKEN EGGS Nurhadi Nurhadi; Kamaruzaman Sijam; Inon Sulaiman
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v4n1.2003.p18-26

Abstract

Citrus tristeza virus (CTV) is one of the most destructive diseases in many citrus growing areas of Indonesia. Effective strategies for controlling CTV depend on diagnostic procedure namely enzyme-linked immunosorbent assay (ELISA). Study aimed to purify the CTV antigen and produced its polyclonal antibody. Virion of the severe CTV isolate designated UPM/ T-002 was concentrated by polyethylene glycol (PEG) precipitation combined with low speed centrifugation. Semipurified antigen was further purified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE). The specific coat protein (CP) band of CTV with molecular weight of 25 kD was excised and eluted using elution buffer containing 0.25 M Tris-HCl pH 6.8 + 0.1% SDS, then used as antigen for injection into 6-month-old female of White Leghorn chicken. Results, showed than the specific polyclonal antibody raised against the 25-kDa CP had a titer of approximately 104, gave low background reaction with healthy plant sap and reacted specifically with CTV isolates. The reaction was equally strong for a severe, a moderate, a mild, and a symptomless isolate, suggesting a broad reaction range of this antibody toward different CTV isolates. Optimal virus titer can be obtained since virus loss during purification could be minimized and the highly purified antigen as an immunogen could be obtained by cutting out the CP band from SDS-PAGE gels. Large amount of highly titer of CTV antibody can be produced in chicken egg. The simplicity of the procedure makes it economically acceptable and technically adoptable because the antibody can be produced in basic laboratory.

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