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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 10, No 1 (2014): April" : 5 Documents clear
Construction of Cry1Ac Plasmid Vector and Its Transformation into Agrobacterium tumefaciens Sri Koerniati; Alifah R. Heritiera
Jurnal AgroBiogen Vol 10, No 1 (2014): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n1.2014.p26-33

Abstract

Introducing cry genesinto rice genome is reported able to produce rice plantresistant to stemborer. DNA sequence encodes cry1Ac genehas been inserted into pGEM4Z, but this construct does nothave a selectable marker gene for selection of transformedplant cells. The research aims were to construct a plasmidvector expressing a cry1Ac gene that has a transformationselectable gene and to transform it into Agrobacteriumtumefaciens. Materials used were pAY560325 binary plasmidvector, pGEM4Z-cry1Ac vector, Escherichia coli strain DH5-αand A. tumefaciens strain LBA4404 competent cells. Themethods consisted of plasmid DNA digestion using HindIIIand EcoRI, electrophoresis, DNA (backbone and insert)dissection from the gel, purification, and ligation using T4DNA ligase. Transformation of ligated DNA into E. coli byheat shock followed by cell plating onto selection medium,colony cultured, DNA isolation, and identification usingrestriction enzymes. Reconfirmation was done by cuttingusing restriction enzyme and PCR using F3 and R3, cry1Acgene specific primers. Research result were DNA fragmentsof 3.8 kb ubiquitin::cry1Ac insert and pAY560325, thebackbone vector, that after ligated and transformed into E.coli produced colonies. One of ten colonies containingplasmid DNA was evidently confirmed and namedpAY560325-cry1Ac. Subsequently, it was transformed into A.tumefaciens by electrophoration method. Plasmid DNA wasisolated from Agrobacterium that after digested with HindIIIand EcoRI produced DNA fragments of 9.44 kb (pAY560325)and 3.814 kb (ubiquitin::cry1Ac). While by PCR, plasmidproduced DNA fragment of about 711 bp. Thus, cry1Acplasmid vector (pAY560325-cry1Ac) was successfullyconstructed and transformed into A. tumefaciens and isready to be transformed into rice genome.
Pemanfaatan Teknik Kultur In Vitro untuk Konservasi Plasma Nutfah Ubi-ubian Nurwita Dewi; Iswari S. Dewi; Ika Roostika
Jurnal AgroBiogen Vol 10, No 1 (2014): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n1.2014.p34-44

Abstract

Except for potato, sweet potato, taro, yam, andcassava, most of tuber crops are considered as underutilizedcrops. However, tuber crops are potential as alternativecarbohydrate sources, so they can be used as food reservesto face global climate change that affects food security incertain area throughout the world, including Indonesia.Having high diversity in tuber crops germplasm, Indonesiamust be able to conserve those germplasm to ensure theiravailability in the future. In the future, without ignoring allthe probable constraints, the prospect in utilization of in vitroculture technique will be higher for improvement ofconservation and management of genetic resources in theform of active and base collections. In this paper, strategy indeveloping in vitro collection of tuber crops germplasm, i.e.slow growth technique for medium term storage andcryopreservation technique for long term storage, isdiscussed including how to analyze genetic stability of thecollections. Several national and international researchcenters dealing with research and development of in vitroconservation technique are presented.
Pendugaan Gen Bph1, bph2, Bph3, dan bph4 pada Galur-galur Padi Terpilih Tahan Hama Wereng Batang Cokelat (Nilaparvata lugens[Stål]) Diani Damayanti; Dwinita W. Utami
Jurnal AgroBiogen Vol 10, No 1 (2014): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n1.2014.p1-8

Abstract

Pests are major constraints to increasingrice production and brown planthoppers/BPH (Nilaparvatalugens [Stål]) is one of the major pests of rice plant.Resistance cultivar is one of the strategies for BPHmanagement. The objective of this research was to analyzethe Bph1, bph2, Bph3, and bph4 gene existence on theselected rice lines using the molecular markers. Thephenotype of the rice lines were tested based on theirresponse to BPH population collected from West Java andCental Java. Molecular markers linked to Bph1, bph2, Bph3,and bph4 were used to characterize the genotypic profilebased on PCR analysis. The results showed that there are sixgenotypes resistant to one of the BPH populations fromWest Java or Central Java. The six rice varieties weredetected to have not only allele of Bph3 gene, but also otherdifferent allele genes. B12344-2D-PN-42-1 and Inpari 13 weredetected to have the alleles of Bph3 dan Bph1 genes.B12512-18-SI-3-3-MR-3-PN-1, B12512-18-SI-3-3-MR-3-PN-1,BMIP46-4-1, and PTB33 were detected to have the alleles ofBph3 and bph2 genes. Meanwhile, B11007E-MR-3-2-PN-2-1-MR-1-2 was detected to have alleles of three genes: Bph3,bph2, and bph4. Nevertheless, this last line had mediumresistance to both BPH populations invested. There is apossibility that the interaction between two genes, Bph3 andbph4, occured which may affect the resistance responses ofrice varieties tested to BPH.
Evaluasi Lapang dan Identifikasi Molekuler Plasma Nutfah Padi terhadap Keracunan Fe Dwinita W. Utami; Ida Hanarida
Jurnal AgroBiogen Vol 10, No 1 (2014): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n1.2014.p9-17

Abstract

Fe toxicity is one of the abiotic constraints thatcan significantly decrease rice production, especially inmarginal wetlands. The use of tolerant varieties can reducethe cost of soil processing and fertilizing. Many accessions ofrice germplasm have potential alleles that can be utilized tocreate new varieties tolerant to Fe toxicity. The objectives ofthis research were to evaluate the Fe toxicity tolerance ofrice germplasm and to analyze the genotype diversity usingSNP markers for OsIRT, Fe toxicity tolerant gene(s). Fetoxicity tolerant rice germplasms were screened in acidmarginal wetlands of Taman Bogo Experimental Station,Indonesian Soil Research Insitute, Lampung Province.Meanwhile, the genotypes performance analysis wasconducted on SNP genotyping analysis using SNP markersfor OsIRT gene(s). Based on phenotypic data of 97accessions, which were clustered into six groups, two ofthem (group 2 and group 5) consisted of the tolerantaccessions at both vegetative and generative stages. Theresults of grouping analysis of genotyping based on SNPmarkers were obtained that there were five genotypegroups: AGT, AAT, GAT, AAC, and GAC. The AGT genotypecluster was dominated by the accessions included in group1. Meanwhile, the GAT genotype cluster consisted of mixedtolerant and untolerant accessions to Fe toxicity. The GACgenotype cluster was dominated by the accessions includedin group 2. The accessions which were included in the besttolerant group, group 5, were separated in differentgenotype cluster. Based on association analysis, among thethree SNP markers, OsIRT1 was the most significant SNPmarker (P value = 0.01) which correlated to Fe toxicitytolerant on vegetative stage. Some of the selectedaccessions that were tolerant to Fe toxicity and had goodagronomic performance on acid soil with high Fe contentwere Ketan Alay, Markuti, Arias Halus, Komas a, Lantiak,and Utri Deli. These local rice accessions have the potentialalleles of OsIRT genes.
Regenerasi Empat Genotipe Gandum (Triticum aestivum L.) Pasca Transformasi Melalui Agrobacterium tumefaciens Apriana, Aniversari; Sisharmini, Atmitri; Fitriyani, Fitriyani; Sustiprijatno, Sustiprijatno
Jurnal AgroBiogen Vol 10, No 1 (2014): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n1.2014.p18-25

Abstract

Genetic transformation ofwheat mediated by Agrobacterium tumefaciens has notbeen established yet. This research aimed to obtain themost responsive wheat genotype to be transformed, toselect the most effective combination of growth regulator forregenerating transforman calli, and to determine thetransformation efficiency. Transformation mediated by A.tumefaciens was performed on four genotypes of wheat,namely Combi, Fasan, Perdix, and Naxos-Wew.Transformed calli with green spots in selection media weretransferred to regeneration media containing 25 mg/lhygromycin, i.e. R1H25 and R2H25. The obtained plantletswere analyzed by PCR using specific primers for hygromycinphosphotransferase gene. The results showed that Fasanwas the most responsive genotype in callus formation(95.47%) and regeneration both in R1H25 (27%) and R2H25(28.6%) media. Fourteen plantlets were succesfullyacclimatized and PCR analysis showed that there were fourpositive transformants containing the hpt gene. The resultsare expected to provide information on the development oftransgenic wheat in Indonesia, specifically in the success ofcallus formation and regeneration of wheat aftertransformation using A. tumefaciens.

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