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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 11, No 2 (2015): Agustus" : 5 Documents clear
Resistance and Phenotypic Character of Chili M2 Mutant Lines Against Chilli Veinal Mottle Virus Ifa Manzila; Neni Gunaeni; Yenni Kusandriani; Tri P. Priyatno
Jurnal AgroBiogen Vol 11, No 2 (2015): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n2.2015.p73-80

Abstract

Chilli veinal mottle virus infection (ChiVMV) could reduce the quality and 60–100% of yield losses of chili. Among the chilivarieties released, no one has been resistant to ChiVMV, mainly due to a high variation of ChiVMV strains and not well mapped.Therefore, finding a new source of ChiVMV resistant genes is pivotal role in order to assembly new varieties. Approach throughin vitro mutation induction using mutagen ethyl methane sulfonate (EMS) is one of the efforts to increase genetic diversity.Previous studies has successfully acquired 800 M2 lines through callus induction of Gelora variety with EMS. This study aimed toobtain M2 lines resistant to ChiVMV and having a good agronomical characters. A total of 800 chili M2 lines that derived from chiliM2 mutations using mutagen EMS has been tested in greenhouse to ChiVMV resistance and studied character phenotype. Theresults showed that of the 800 lines, there were 28 strains obtained showed a response tolerant and resistant to ChiVMV. Eightmutant lines of which have good agronomic characters. The mutant lines are M2.100, M2.108, M2.200, M2. 122, M2.238, M2.353,M2.420, and M2.517. Eight lines will be selected and further observed to obtain chili promising lines that are resistant to ChiVMVand high yielding.
Development of SSR Marker Set to Identify Fourty Two Indonesian Soybean Varieties Andari Risliawati; Eny I. Riyanti; Puji Lestari; Dwinita W. Utami; Tiur S. Silitonga
Jurnal AgroBiogen Vol 11, No 2 (2015): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n2.2015.p49-58

Abstract

Profile of molecular marker can be used for variety identification, genetic purity monitoring of germplasm and additionalrequirement in proposing intellectual property protection. DNA fingerprinting of soybean had been applied at the ICABIOGRADIAARDsince 2004 using simple sequence repeat (SSR) markers which were run automatically by CEQ 8000 Genetic Analyzerplatform based on capillary electrophoresis system. This method had produced unique DNA fingerprints of the varieties tested,but the marker set to efficiently identify the varieties had not yet been developed. This study aimed to develop a set of SSRmarkers as a tool to identify the Indonesian soybean varieties. Fourty two soybean varieties were analyzed using 14 random SSRmarkersA total of 168 alleles that were obtained from the polymorphism analysis. The average of polymorphic informationcontent (PIC) value observed was 0.7337 per SSR locus. Based on marker reproducibility rate, PIC value, number of rare alleles,frequency of dominant alleles, and percentage of SSR fragment detected by genetic analyzer, we identified five SSR markers i.e.Satt414, Satt147, Satt308, Satt009, and Satt516 as a SSR marker set to be used for soybean variety identification purposes. Thismarker set was used to develop the identity (ID) of the 42 Indonesian soybean varieties.
Keragaman Genetika Empat Belas Aksesi Kentang (Solanum tuberosum L.) Berdasarkan Marka SSR dan STS (Genetic Diversity of Fourteen Potato Accessions Based on SSR and STS Markers) Nugroho, Kristianto; Reflinur, Reflinur; Lestari, Puji; Rosdianti, Ida; Terryana, Rerenstradika T.; Kusmana, Kusmana; Tasma, I Made
Jurnal AgroBiogen Vol 11, No 2 (2015): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n2.2015.p41-48

Abstract

Potato is one of high economically horticultural plant. The increasing of national consumption of potato becomes a challenge forpotato breeders. The success of breeding programs is depending on availability of genetic diversity. The aim of this research wasto analyze the genetic diversity of fourteen accessions of potato by using SSR and STS markers. PCR analysis was scored as binerdata and the collected data was analyzed using NTSYS and PowerMarker. The result showed that there were 63% polymorphic(12 markers) of total markers. As many as 60 alleles with the size of 200–500 bp were identified by a range of 2–9 alleles perlocus. The polymorphism level was 0.59 (0.36–0.74). Result also showed the average of major allele frequency was 49.42%(35.71–63.64%). Nine markers which have polymorphism level more than 0.5 could be used to detect genetic diversity of potato.The average of genetic diversity index was 0.65. Cluster analysis showed that 14 accessions of potato were split in two groups(coefficient 0.70). The first groups consisted of Atlantik, GM 05, Granola Kembang Merbabu 17, and the second groups consist ofRepita, Maglia, Medians, CIP397078.7, CIP392781.1, Margahayu, Granola, CIP394613.139, Amabile, and Tenggo. The informationof genetic diversity of this germplasm could be used as a preliminary basis for choosing crossing parents in potato breeding inIndonesia.
Identifikasi cDNA Gen RB pada Tanaman Kentang Produk Rekayasa Genetika Katahdin SP951 Hadiarto, Toto; Listanto, Edy; Riyanti, Eny I.
Jurnal AgroBiogen Vol 11, No 2 (2015): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n2.2015.p59-64

Abstract

To overcome the negative impact of the fungicide application, RB gene, originated from Solanum bulbocastanum, a resistancegene to the disease has been introduced to the genome of potato plant Katahdin, which was later named as Katahdin SP951.The aims of the research were to confirm the RB gene in the genetically modified plant (GMP) Katahdin SP951 throughresequencing the RB gene in the Katahdin SP951 genome and to align the RB genes isolated from the transgenic Katahdin SP951and the cloning vector pLCD0454, which was used the vector to transform Katahdin cultivar. The RB gene has high similarity toother resistance genes in potato plants, and therefore nontransgenic Katahdin, and Solanum bulbocastanum were used in theanalysis as the negative and positive control, respectively. Seven pairs of specific primers, which could differentiate the RB genewere used for gene sequencing studies. The sequencing experiments obtained 2913 bases which showed 100% similarity to theRB sequence isolated from the pLCD04541 plasmid. It is concluded that the RB gene in the Katahdin SP951 did not experiencemutation during transformation process and integration into the Katahdin genome.
Teknik PCR Kualitatif untuk Deteksi Produk Rekayasa Genetika Jagung Event BT11 dan GA21 Bahagiawati Bahagiawati; Reflinur Reflinur; Tri J. Santoso
Jurnal AgroBiogen Vol 11, No 2 (2015): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n2.2015.p65-72

Abstract

In some countries, including Indonesia, labelling of GMO products is mandatory for giving consumers the right to choosebetween GMOs and conventional products. Therefore, development of methodology that can detect a specific geneticallymodified (GM) crops and to verify the absence or presence of GM material in a product including raw materials (e.g. grains)and/or their derivatives is needed. The objectives of this study were to find the most efficient screening methods to detectwhether or not a product is GM material and to develop a specific detection method to identify GM product BT11 and GA21. Inaddition, present study was also aimed to obtain a duplex detection method for both GM products. Two GM-maize, including theBT11 and GA21 lines of maize (Zea mays L.), and one plant, namely NK11 as the nontransgenic control, were used as plantgenetic materials in the event-specific detection of maize. The target gene from each sample was amplified in different reaction(simplex) using both the event specific primer and the endogenous maize reference, Zein, as internal control. Furthermore, induplex PCR, two targets were simultaneously amplified in the same reaction. The results showed that detection method of theGM product obtained from present study enabled us to screen the GM products and specifically the event of BT11 and GA21using simplex and duplex methods. The duplex method is more efficient because it can detect two GM crops in one timecompared to simplex method that only can detect GM crop one by one.

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